Restriction map for
pF1KE2752
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbeI BbsI BcgI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrGI BssHII
Bst1107I BstAPI BstZ17I CelII CpoI
Eam1105I Eco47III EcoRV FseI FspI
HaeII HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1243 GTCTAC GT^[AC][GT]AC
AccII 58 CGCG CG^CG
AcyI 52 GGCGTC G[GA]^CG[CT]C
AflII 43 CTTAAG C^TTAAG
AspI 2874 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2234 CCCGAG C^[CT]CG[GA]G
BbvCI 2451 GCTGAGG CCTCAGC(-5/-2)
BciVI 310 GTATCC GTATCC(6/5)
BfrI 43 CTTAAG C^TTAAG
BglII 3374 AGATCT A^GATCT
BsaHI 52 GGCGTC G[GA]^CG[CT]C
BsaI 952 GAGACC GGTCTC(1/5)
BsiEI 946 CGGCCG CG[GA][CT]^CG
BsoBI 2234 CCCGAG C^[CT]CG[GA]G
BspDI 3115 ATCGAT AT^CGAT
BsrBI 943 GAGCGG CCGCTC(-3/-3)
BsrFI 49 ACCGGC [GA]^CCGG[CT]
BssSI 2092 CTCGTG CACGAG(-5/-1)
BstBI 2180 TTCGAA TT^CGAA
BstEII 771 GGTTACC G^GT[ACGT]ACC
BstPI 771 GGTTACC G^GT[ACGT]ACC
BstUI 58 CGCG CG^CG
BstXI 403 CCAGCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1822 GCGC GCG^C
Cfr10I 49 ACCGGC [GA]^CCGG[CT]
ClaI 3115 ATCGAT AT^CGAT
DraI 2532 TTTAAA TTT^AAA
DrdI 3463 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 946 CGGCCG C^GGCCG
EclXI 946 CGGCCG C^GGCCG
Eco52I 946 CGGCCG C^GGCCG
EcoO65I 771 GGTTACC G^GT[ACGT]ACC
EcoRI 1775 GAATTC G^AATTC
HgaI 53 GCGTC GACGC(5/10)
HhaI 1822 GCGC GCG^C
Hin1I 52 GGCGTC G[GA]^CG[CT]C
HinP1I 1822 GCGC G^CGC
MvnI 58 CGCG CG^CG
NdeI 2063 CATATG CA^TATG
NspV 2180 TTCGAA TT^CGAA
PflFI 2874 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 2470 CAGCTG CAG^CTG
ScaI 1356 AGTACT AGT^ACT
SexAI 1463 ACCTGGT A^CC[AT]GGT
SfuI 2180 TTCGAA TT^CGAA
Tth111I 2874 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1515 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 546 GGTACC G^GTACC
1495 GGTACC
AflIII 89 ACGTGT A^C[GA][CT]GT
104 ACACGT
Asp718I 546 GGTACC G^GTACC
1495 GGTACC
BalI 2096 TGGCCA TGG^CCA
3407 TGGCCA
BanII 1101 GAGCCC G[GA]GC[CT]^C
2192 GGGCTC
BbrPI 105 CACGTG CAC^GTG
2042 CACGTG
BclI 1328 TGATCA T^GATCA
2075 TGATCA
BglI 924 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2278 GCCCGTTGGGC
BsmBI 1135 GAGACG CGTCTC(1/5)
1666 GAGACG
BsrDI 145 CATTGC GCAATG(2/0)
3077 CATTGC
DraIII 958 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1462 CACCTGGTG
EcoT22I 231 ATGCAT ATGCA^T
329 ATGCAT
FauI 1687 CCCGC CCCGC(4/6)
120 GCGGG
FbaI 1328 TGATCA T^GATCA
2075 TGATCA
HincII 1631 GTCAAC GT[CT]^[GA]AC
2392 GTTGAC
HindII 1631 GTCAAC GT[CT]^[GA]AC
2392 GTTGAC
HindIII 2536 AAGCTT A^AGCTT
3290 AAGCTT
KpnI 546 GGTACC GGTAC^C
1495 GGTACC
MluNI 2096 TGGCCA TGG^CCA
3407 TGGCCA
MscI 2096 TGGCCA TGG^CCA
3407 TGGCCA
MslI 1591 CACGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2250 CATAGCTGTG
MspA1I 983 CAGCGG C[AC]G^C[GT]G
2470 CAGCTG
NsiI 231 ATGCAT ATGCA^T
329 ATGCAT
PflMI 1341 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1500 CCAACCTGTGG
PmaCI 105 CACGTG CAC^GTG
2042 CACGTG
PmlI 105 CACGTG CAC^GTG
2042 CACGTG
PpuMI 834 GGGACCC [GA]G^G[AT]CC[CT]
1700 AGGTCCC
PshAI 48 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1312 GACAAGGGTC
SapI 1272 GAAGAGC GCTCTTC(1/4)
1303 GCTCTTC
SfcI 566 CTGTAG C^T[GA][CT]AG
2085 CTATAG
SmlI 43 CTTAAG C^T[CT][GA]AG
1648 CTTGAG
SphI 327 GCATGC GCATG^C
2957 GCATGC
Van91I 1341 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1500 CCAACCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BamHI BanI BbvI BcnI BfaI
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII