################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BbeI BbsI BcgI BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrGI BssHII Bst1107I BstAPI BstZ17I CelII CpoI Eam1105I Eco47III EcoRV FseI FspI HaeII HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 1243 GTCTAC GT^[AC][GT]AC AccII 58 CGCG CG^CG AcyI 52 GGCGTC G[GA]^CG[CT]C AflII 43 CTTAAG C^TTAAG AspI 2874 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2234 CCCGAG C^[CT]CG[GA]G BbvCI 2451 GCTGAGG CCTCAGC(-5/-2) BciVI 310 GTATCC GTATCC(6/5) BfrI 43 CTTAAG C^TTAAG BglII 3374 AGATCT A^GATCT BsaHI 52 GGCGTC G[GA]^CG[CT]C BsaI 952 GAGACC GGTCTC(1/5) BsiEI 946 CGGCCG CG[GA][CT]^CG BsoBI 2234 CCCGAG C^[CT]CG[GA]G BspDI 3115 ATCGAT AT^CGAT BsrBI 943 GAGCGG CCGCTC(-3/-3) BsrFI 49 ACCGGC [GA]^CCGG[CT] BssSI 2092 CTCGTG CACGAG(-5/-1) BstBI 2180 TTCGAA TT^CGAA BstEII 771 GGTTACC G^GT[ACGT]ACC BstPI 771 GGTTACC G^GT[ACGT]ACC BstUI 58 CGCG CG^CG BstXI 403 CCAGCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 1822 GCGC GCG^C Cfr10I 49 ACCGGC [GA]^CCGG[CT] ClaI 3115 ATCGAT AT^CGAT DraI 2532 TTTAAA TTT^AAA DrdI 3463 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 946 CGGCCG C^GGCCG EclXI 946 CGGCCG C^GGCCG Eco52I 946 CGGCCG C^GGCCG EcoO65I 771 GGTTACC G^GT[ACGT]ACC EcoRI 1775 GAATTC G^AATTC HgaI 53 GCGTC GACGC(5/10) HhaI 1822 GCGC GCG^C Hin1I 52 GGCGTC G[GA]^CG[CT]C HinP1I 1822 GCGC G^CGC MvnI 58 CGCG CG^CG NdeI 2063 CATATG CA^TATG NspV 2180 TTCGAA TT^CGAA PflFI 2874 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 2470 CAGCTG CAG^CTG ScaI 1356 AGTACT AGT^ACT SexAI 1463 ACCTGGT A^CC[AT]GGT SfuI 2180 TTCGAA TT^CGAA Tth111I 2874 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1515 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 546 GGTACC G^GTACC 1495 GGTACC AflIII 89 ACGTGT A^C[GA][CT]GT 104 ACACGT Asp718I 546 GGTACC G^GTACC 1495 GGTACC BalI 2096 TGGCCA TGG^CCA 3407 TGGCCA BanII 1101 GAGCCC G[GA]GC[CT]^C 2192 GGGCTC BbrPI 105 CACGTG CAC^GTG 2042 CACGTG BclI 1328 TGATCA T^GATCA 2075 TGATCA BglI 924 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2278 GCCCGTTGGGC BsmBI 1135 GAGACG CGTCTC(1/5) 1666 GAGACG BsrDI 145 CATTGC GCAATG(2/0) 3077 CATTGC DraIII 958 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1462 CACCTGGTG EcoT22I 231 ATGCAT ATGCA^T 329 ATGCAT FauI 1687 CCCGC CCCGC(4/6) 120 GCGGG FbaI 1328 TGATCA T^GATCA 2075 TGATCA HincII 1631 GTCAAC GT[CT]^[GA]AC 2392 GTTGAC HindII 1631 GTCAAC GT[CT]^[GA]AC 2392 GTTGAC HindIII 2536 AAGCTT A^AGCTT 3290 AAGCTT KpnI 546 GGTACC GGTAC^C 1495 GGTACC MluNI 2096 TGGCCA TGG^CCA 3407 TGGCCA MscI 2096 TGGCCA TGG^CCA 3407 TGGCCA MslI 1591 CACGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2250 CATAGCTGTG MspA1I 983 CAGCGG C[AC]G^C[GT]G 2470 CAGCTG NsiI 231 ATGCAT ATGCA^T 329 ATGCAT PflMI 1341 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1500 CCAACCTGTGG PmaCI 105 CACGTG CAC^GTG 2042 CACGTG PmlI 105 CACGTG CAC^GTG 2042 CACGTG PpuMI 834 GGGACCC [GA]G^G[AT]CC[CT] 1700 AGGTCCC PshAI 48 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1312 GACAAGGGTC SapI 1272 GAAGAGC GCTCTTC(1/4) 1303 GCTCTTC SfcI 566 CTGTAG C^T[GA][CT]AG 2085 CTATAG SmlI 43 CTTAAG C^T[CT][GA]AG 1648 CTTGAG SphI 327 GCATGC GCATG^C 2957 GCATGC Van91I 1341 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1500 CCAACCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BamHI BanI BbvI BcnI BfaI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII