################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BfrI BlnI BpuAI BsaAI BsaBI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MunI MvnI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 3269 TCCGGA T^CCGGA BbeI 2439 GGCGCC GGCGC^C BsaWI 3269 TCCGGA [AT]^CCGG[AT] BseAI 3269 TCCGGA T^CCGGA BspEI 3269 TCCGGA T^CCGGA BsrFI 1749 GCCGGC [GA]^CCGG[CT] BssSI 1477 CACGAG CACGAG(-5/-1) Cfr10I 1749 GCCGGC [GA]^CCGG[CT] EcoT22I 1522 ATGCAT ATGCA^T FauI 3108 GCGGG CCCGC(4/6) HaeII 2439 GGCGCC [GA]GCGC^[CT] HgaI 1795 GCGTC GACGC(5/10) KasI 2439 GGCGCC G^GCGCC MaeII 2455 ACGT A^CGT MroI 3269 TCCGGA T^CCGGA MslI 953 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1749 GCCGGC GCC^GGC NarI 2439 GGCGCC GG^CGCC NcoI 3251 CCATGG C^CATGG NdeI 3586 CATATG CA^TATG NgoMIV 1749 GCCGGC G^CCGGC NheI 774 GCTAGC G^CTAGC NsiI 1522 ATGCAT ATGCA^T PshAI 809 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 1707 CCTGCAGG CCTGCA^GG ScaI 213 AGTACT AGT^ACT SexAI 1936 ACCAGGT A^CC[AT]GGT SfiI 2751 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2439 GGCGCC GGC^GCC SmaI 115 CCCGGG CCC^GGG Sse8387I 1707 CCTGCAGG CCTGCA^GG XmaCI 115 CCCGGG C^CCGGG XmaI 115 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 374 GTCTAC GT^[AC][GT]AC 2457 GTCTAC AcsI 606 GAATTT [GA]^AATT[CT] 2499 GAATTT AcyI 1794 GGCGTC G[GA]^CG[CT]C 2439 GGCGCC AflIII 235 ACATGT A^C[GA][CT]GT 297 ACATGT AhdI 2085 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2737 GACAGACTGTC ApoI 606 GAATTT [GA]^AATT[CT] 2499 GAATTT AspEI 2085 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2737 GACAGACTGTC BbvCI 1618 CCTCAGC CCTCAGC(-5/-2) 3183 CCTCAGC BglII 2603 AGATCT A^GATCT 3437 AGATCT BsaHI 1794 GGCGTC G[GA]^CG[CT]C 2439 GGCGCC BsaI 1702 GAGACC GGTCTC(1/5) 1076 GGTCTC BspLU11I 235 ACATGT A^CATGT 297 ACATGT BstXI 1791 CCAGGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1907 CCAACACTCTGG CfoI 2440 GCGC GCG^C 3336 GCGC Eam1105I 2085 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2737 GACAGACTGTC EarI 1442 CTCTTC CTCTTC(1/4) 30 GAAGAG HhaI 2440 GCGC GCG^C 3336 GCGC Hin1I 1794 GGCGTC G[GA]^CG[CT]C 2439 GGCGCC HinP1I 2440 GCGC G^CGC 3336 GCGC Ksp632I 1442 CTCTTC CTCTTC(1/4) 30 GAAGAG MseI 2411 TTAA T^TAA 2723 TTAA PpuMI 1004 AGGTCCC [GA]G^G[AT]CC[CT] 3290 AGGACCC SmlI 217 CTCAAG C^T[CT][GA]AG 2599 CTCAAG TaqI 2690 TCGA T^CGA 2862 TCGA TfiI 740 GATTC G^A[AT]TC 2574 GATTC Tru9I 2411 TTAA T^TAA 2723 TTAA TthHB8I 2690 TCGA T^CGA 2862 TCGA XcmI 1302 CCAAGTCCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3013 CCAGGGGGAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CelII Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI Van91I XhoII