Restriction map for
pF1KE3543
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbrPI BbsI
BcgI BciVI BfrI BlnI BpuAI
BsaAI BsaBI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MunI MvnI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 3269 TCCGGA T^CCGGA
BbeI 2439 GGCGCC GGCGC^C
BsaWI 3269 TCCGGA [AT]^CCGG[AT]
BseAI 3269 TCCGGA T^CCGGA
BspEI 3269 TCCGGA T^CCGGA
BsrFI 1749 GCCGGC [GA]^CCGG[CT]
BssSI 1477 CACGAG CACGAG(-5/-1)
Cfr10I 1749 GCCGGC [GA]^CCGG[CT]
EcoT22I 1522 ATGCAT ATGCA^T
FauI 3108 GCGGG CCCGC(4/6)
HaeII 2439 GGCGCC [GA]GCGC^[CT]
HgaI 1795 GCGTC GACGC(5/10)
KasI 2439 GGCGCC G^GCGCC
MaeII 2455 ACGT A^CGT
MroI 3269 TCCGGA T^CCGGA
MslI 953 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1749 GCCGGC GCC^GGC
NarI 2439 GGCGCC GG^CGCC
NcoI 3251 CCATGG C^CATGG
NdeI 3586 CATATG CA^TATG
NgoMIV 1749 GCCGGC G^CCGGC
NheI 774 GCTAGC G^CTAGC
NsiI 1522 ATGCAT ATGCA^T
PshAI 809 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1707 CCTGCAGG CCTGCA^GG
ScaI 213 AGTACT AGT^ACT
SexAI 1936 ACCAGGT A^CC[AT]GGT
SfiI 2751 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2439 GGCGCC GGC^GCC
SmaI 115 CCCGGG CCC^GGG
Sse8387I 1707 CCTGCAGG CCTGCA^GG
XmaCI 115 CCCGGG C^CCGGG
XmaI 115 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 374 GTCTAC GT^[AC][GT]AC
2457 GTCTAC
AcsI 606 GAATTT [GA]^AATT[CT]
2499 GAATTT
AcyI 1794 GGCGTC G[GA]^CG[CT]C
2439 GGCGCC
AflIII 235 ACATGT A^C[GA][CT]GT
297 ACATGT
AhdI 2085 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2737 GACAGACTGTC
ApoI 606 GAATTT [GA]^AATT[CT]
2499 GAATTT
AspEI 2085 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2737 GACAGACTGTC
BbvCI 1618 CCTCAGC CCTCAGC(-5/-2)
3183 CCTCAGC
BglII 2603 AGATCT A^GATCT
3437 AGATCT
BsaHI 1794 GGCGTC G[GA]^CG[CT]C
2439 GGCGCC
BsaI 1702 GAGACC GGTCTC(1/5)
1076 GGTCTC
BspLU11I 235 ACATGT A^CATGT
297 ACATGT
BstXI 1791 CCAGGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1907 CCAACACTCTGG
CfoI 2440 GCGC GCG^C
3336 GCGC
Eam1105I 2085 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2737 GACAGACTGTC
EarI 1442 CTCTTC CTCTTC(1/4)
30 GAAGAG
HhaI 2440 GCGC GCG^C
3336 GCGC
Hin1I 1794 GGCGTC G[GA]^CG[CT]C
2439 GGCGCC
HinP1I 2440 GCGC G^CGC
3336 GCGC
Ksp632I 1442 CTCTTC CTCTTC(1/4)
30 GAAGAG
MseI 2411 TTAA T^TAA
2723 TTAA
PpuMI 1004 AGGTCCC [GA]G^G[AT]CC[CT]
3290 AGGACCC
SmlI 217 CTCAAG C^T[CT][GA]AG
2599 CTCAAG
TaqI 2690 TCGA T^CGA
2862 TCGA
TfiI 740 GATTC G^A[AT]TC
2574 GATTC
Tru9I 2411 TTAA T^TAA
2723 TTAA
TthHB8I 2690 TCGA T^CGA
2862 TCGA
XcmI 1302 CCAAGTCCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3013 CCAGGGGGAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CelII Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I Tsp509I TspRI Van91I XhoII