Restriction map for
pFN21AA1373
(Circular) (Six-base) MAPSORT of: pFN21AA1373.seq Check: 7148 from: 1 to: 6088
pFN21AA1373 6088 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2354 3154 4151 5535 5803 1632
Size: 722 800 997 1384 268 1917
Fragments arranged by size:
1917 1384 997 800 722 268
AfeI AGC'GCT
Cuts at: 2632 2632
Size: 6088
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2306 2536 3831 5403 2306
Size: 230 1295 1572 2991
Fragments arranged by size:
2991 1572 1295 230
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 2026 2212 2243 2442 2782 2837 2918 3266
Size: 533 186 31 199 340 55 81 348
Cuts at: 3266 3397 3623 3775 5884 1493
Size: 131 226 152 2109 1697
Fragments arranged by size:
2109 1697 533 348 340 226 199 186
152 131 81 55 31
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2574 3413 1870
Size: 73 631 839 4545
Fragments arranged by size:
4545 839 631 73
BamHI G'GATC_C
Cuts at: 3039 3418 3785 3039
Size: 379 367 5342
Fragments arranged by size:
5342 379 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3409 4864 618
Size: 325 217 627 1622 1455 1842
Fragments arranged by size:
1842 1622 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3246 3312 3407 1391
Size: 1855 66 95 4072
Fragments arranged by size:
4072 1855 95 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2142 928
Size: 971 243 4874
Fragments arranged by size:
4874 971 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2824 6083 2824
Size: 3259 2829
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 2101 4149 5305 1790
Size: 311 2048 1156 2573
Fragments arranged by size:
2573 2048 1156 311
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3819 4777 442
Size: 416 53 83 399 24 2402 958 1753
Fragments arranged by size:
2402 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2724 1056
Size: 1668 4420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2187 4748 1239
Size: 948 2561 2579
Fragments arranged by size:
2579 2561 948
Bpu10I CC'TnA_GC
Cuts at: 2075 2102 2075
Size: 27 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2540 3215 4213 5078 5376 2540
Size: 675 998 865 298 3252
Fragments arranged by size:
3252 998 865 675 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2189 3895 4277 4912
Size: 53 83 186 936 656 1706 382 635
Cuts at: 4912 275
Size: 1451
Fragments arranged by size:
1706 1451 936 656 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3006 3036 1178
Size: 30 165 30 1603 30 4230
Fragments arranged by size:
4230 1603 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2721 3014 1186
Size: 1535 293 4260
Fragments arranged by size:
4260 1535 293
BseYI C'CCAG_C
Cuts at: 5291 5787 5291
Size: 496 5592
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2319 1163
Size: 573 583 4932
Fragments arranged by size:
4932 583 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4149 4234 5305 4149
Size: 85 1071 4932
Fragments arranged by size:
4932 1071 85
BsmI GAATG_Cn'
Cuts at: 2345 3599 3692 2345
Size: 1254 93 4741
Fragments arranged by size:
4741 1254 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 3246 3312 3407 4149 4234 5305
Size: 399 311 1145 66 95 742 85 1071
Cuts at: 5305 1391
Size: 2174
Fragments arranged by size:
2174 1145 1071 742 399 311 95 85
66
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 844
Size: 2599 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2300 3126 4583 4757 65
Size: 1183 1052 826 1457 174 1396
Fragments arranged by size:
1457 1396 1183 1052 826 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4732 4919 1700
Size: 110 2922 187 2869
Fragments arranged by size:
2922 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 2544 4892 2544
Size: 2348 3740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2824 3039 3354 3418 3785 4170 4187 5639
Size: 852 215 315 64 367 385 17 1452
Cuts at: 5639 5725 5737 6083 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 852 385 367 346 315 215
86 64 17 12
Bsu36I CC'TnA_GG
Cuts at: 2602 2602
Size: 6088
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4597
Fragments arranged by size:
4597 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2818 487
Size: 683 56 1592 3757
Fragments arranged by size:
3757 1592 683 56
BtsI GCAGTG_nn'
Cuts at: 3291 3604 4398 4418 3291
Size: 313 794 20 4961
Fragments arranged by size:
4961 794 313 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 2382 3392 2382
Size: 1010 5078
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053
Size: 54 1425 50 275 18 1705 889 1629
Cuts at: 6053 8
Size: 43
Fragments arranged by size:
1705 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2079 4018 1353
Size: 726 1939 3423
Fragments arranged by size:
3423 1939 726
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2354 3154 4151 4748 5535 5803
Size: 393 555 167 800 997 597 787 268
Cuts at: 5803 1239
Size: 1524
Fragments arranged by size:
1524 997 800 787 597 555 393 268
167
EcoO109I rG'GnC_Cy
Cuts at: 2745 2745
Size: 6088
EcoRI G'AATT_C
Cuts at: 2026 3397 3775 2026
Size: 1371 378 4339
Fragments arranged by size:
4339 1371 378
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 2634 5235 1678
Size: 956 2601 2531
Fragments arranged by size:
2601 2531 956
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3330 3362 4729 4761 4803
Size: 32 135 32 1758 32 1367 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2626 1758 1367 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2108 2267 748
Size: 1360 159 4569
Fragments arranged by size:
4569 1360 159
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 4046 4405 4564 518
Size: 648 898 1982 359 159 2042
Fragments arranged by size:
2042 1982 898 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2759 3504 4181 5329 5574 1606
Size: 1153 745 677 1148 245 2120
Fragments arranged by size:
2120 1153 1148 745 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4597
Fragments arranged by size:
4597 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 2720 1052
Size: 1668 4420
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 3193 3446 4991 1132
Size: 97 1964 253 1545 2229
Fragments arranged by size:
2229 1964 1545 253 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6088
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 2238 2249 3658 2238
Size: 11 1409 4668
Fragments arranged by size:
4668 1409 11
PstI C_TGCA'G
Cuts at: 830 3440 830
Size: 2610 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 2759 2759
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SapI GCTCTTCn'nnn_
Cuts at: 2079 2079
Size: 6088
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 3436 4571 5252 5443 826
Size: 219 2391 1135 681 191 1471
Fragments arranged by size:
2391 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 3194 4192 5093 5355 820
Size: 197 926 612 639 998 901 262 1553
Fragments arranged by size:
1553 998 926 901 639 612 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3193 3446 1132
Size: 2061 253 3774
Fragments arranged by size:
3774 2061 253
SspI AAT'ATT
Cuts at: 5 52 2411 4012 5
Size: 47 2359 1601 2081
Fragments arranged by size:
2359 2081 1601 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2004 2351 3237 3527 513
Size: 552 939 347 886 290 3074
Fragments arranged by size:
3074 939 886 552 347 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4115 4132 4285 4470 4915 325
Size: 1255 2535 17 153 185 445 1498
Fragments arranged by size:
2535 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6048 6080 6048
Size: 32 6056
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 2268 4217 2268
Size: 1949 4139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI