Restriction map for
pFN21AE2057
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglI BlpI Bpu1102I BsaAI
BsaI BsmBI BsmI BspLU11I BssHII
Bst1107I BstAPI BstBI BstZ17I CelII
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SfcI SfiI
SfoI SfuI SmlI SnaBI SpeI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 1709 TCCGGA T^CCGGA
AgeI 724 ACCGGT A^CCGGT
AlwNI 558 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1633 CCTGAGG CC^T[ACGT]AGG
Aor51HI 846 AGCGCT AGC^GCT
AspHI 85 GAGCTC G[AT]GC[AT]^C
AvrII 1944 CCTAGG C^CTAGG
BanI 1999 GGCACC G^G[CT][GA]CC
BciVI 1530 GGATAC GTATCC(6/5)
BlnI 1944 CCTAGG C^CTAGG
BsaBI 1707 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1709 TCCGGA T^CCGGA
BseRI 369 CTCCTC GAGGAG(10/8)
BsiEI 1185 CGACCG CG[GA][CT]^CG
BsiHKAI 85 GAGCTC G[AT]GC[AT]^C
BsmAI 172 GTCTC GTCTC(1/5)
Bsp1407I 1590 TGTACA T^GTACA
BspDI 2065 ATCGAT AT^CGAT
BspEI 1709 TCCGGA T^CCGGA
BsrGI 1590 TGTACA T^GTACA
BssSI 1814 CTCGTG CACGAG(-5/-1)
Bsu36I 1633 CCTGAGG CC^T[ACGT]AGG
ClaI 2065 ATCGAT AT^CGAT
DsaI 944 CCATGG C^C[GA][CT]GG
Eco47III 846 AGCGCT AGC^GCT
Eco81I 1633 CCTGAGG CC^T[ACGT]AGG
EcoNI 957 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1233 GAATTC G^AATTC
EcoRV 1978 GATATC GAT^ATC
HaeII 846 AGCGCT [GA]GCGC^[CT]
HincII 1495 GTCAAC GT[CT]^[GA]AC
HindII 1495 GTCAAC GT[CT]^[GA]AC
MamI 1707 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1709 TCCGGA T^CCGGA
NaeI 478 GCCGGC GCC^GGC
NcoI 944 CCATGG C^CATGG
NgoMIV 478 GCCGGC G^CCGGC
PinAI 724 ACCGGT A^CCGGT
PvuII 1141 CAGCTG CAG^CTG
SacI 85 GAGCTC GAGCT^C
SapI 1845 GCTCTTC GCTCTTC(1/4)
SexAI 1499 ACCTGGT A^CC[AT]GGT
SgrAI 723 CACCGGTG C[GA]^CCGG[CT]G
SphI 1472 GCATGC GCATG^C
SspBI 1590 TGTACA T^GTACA
TfiI 864 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1303 GACGTC GACGT^C
1404 GACGTC
AccII 1170 CGCG CG^CG
1401 CGCG
AcyI 1303 GACGTC G[GA]^CG[CT]C
1404 GACGTC
ApaI 1029 GGGCCC GGGCC^C
1948 GGGCCC
Asp700I 36 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
547 GAAGACATTC
AvaI 872 CCCGGG C^[CT]CG[GA]G
1964 CCCGGG
BalI 620 TGGCCA TGG^CCA
810 TGGCCA
BfaI 10 CTAG C^TAG
1945 CTAG
BglII 347 AGATCT A^GATCT
1661 AGATCT
BsaHI 1303 GACGTC G[GA]^CG[CT]C
1404 GACGTC
BsaWI 724 ACCGGT [AT]^CCGG[AT]
1709 TCCGGA
BsgI 1138 GTGCAG GTGCAG(16/14)
1837 GTGCAG
BsiWI 1037 CGTACG C^GTACG
1667 CGTACG
BsoBI 872 CCCGGG C^[CT]CG[GA]G
1964 CCCGGG
BspHI 128 TCATGA T^CATGA
900 TCATGA
BspMI 1050 GCAGGT ACCTGC(4/8)
1259 GCAGGT
BsrBI 711 GAGCGG CCGCTC(-3/-3)
1759 GAGCGG
BstEII 439 GGTAACC G^GT[ACGT]ACC
1918 GGTAACC
BstPI 439 GGTAACC G^GT[ACGT]ACC
1918 GGTAACC
BstUI 1170 CGCG CG^CG
1401 CGCG
EcoO65I 439 GGTAACC G^GT[ACGT]ACC
1918 GGTAACC
HgaI 604 GACGC GACGC(5/10)
428 GCGTC
Hin1I 1303 GACGTC G[GA]^CG[CT]C
1404 GACGTC
MaeI 10 CTAG C^TAG
1945 CTAG
MluNI 620 TGGCCA TGG^CCA
810 TGGCCA
MscI 620 TGGCCA TGG^CCA
810 TGGCCA
MslI 486 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1716 CACAGCGGTG
MspA1I 1141 CAGCTG C[AC]G^C[GT]G
1718 CAGCGG
MvnI 1170 CGCG CG^CG
1401 CGCG
NspI 1154 ACATGC [GA]CATG^[CT]
1472 GCATGC
RcaI 128 TCATGA T^CATGA
900 TCATGA
SmaI 872 CCCGGG CCC^GGG
1964 CCCGGG
StuI 38 AGGCCT AGG^CCT
138 AGGCCT
XcmI 1616 CCACAATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1784 CCACCTTGACCCTGG
XmaCI 872 CCCGGG C^CCGGG
1964 CCCGGG
XmaI 872 CCCGGG C^CCGGG
1964 CCCGGG
XmnI 36 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
547 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbsI BbvI
BclI BcnI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII