HCRTR2 ORF clone FHC02305

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02305

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Clone info.

Species

human

Clone Name

pFN21AB7185 Sequence (with vector) Restriction Map

Product ID

FHC02305

Flexi Clone PID

FXC02305

ORF Sequence

DNA sequence (1332 bp)
Protein sequence (444 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463763]

Gene

KIBB7185

Symbol

HCRTR2

Alias : ORXR2, OX2R, OXR2

Description

hypocretin (orexin) receptor 2

Original Clone ID

oc00489

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1332 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 444 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4956.1 2.3e-143 100.0 HCRTR2 CCDS344.1 3.2e-88 69.2 HCRTR1 CCDS3552.1 8.7e-21 32.1 NPFFR2 CCDS47072.1 8.8e-21 32.1 NPFFR2 CCDS3551.1 1e-20 32.1 NPFFR2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000204 26 37 PR01064 Orexin receptor IPR000204 39 55 PR01064 Orexin receptor IPR000276 56 80 PR00237 GPCR IPR000276 89 110 PR00237 GPCR IPR000204 107 123 PR01064 Orexin receptor IPR000276 134 156 PR00237 GPCR IPR000204 159 170 PR01064 Orexin receptor IPR000276 168 189 PR00237 GPCR IPR000204 198 214 PR01064 Orexin receptor IPR000204 214 232 PR01064 Orexin receptor IPR000276 224 247 PR00237 GPCR IPR000204 247 267 PR01064 Orexin receptor IPR000204 288 304 PR01064 Orexin receptor IPR000276 302 326 PR00237 GPCR IPR000204 327 351 PR01064 Orexin receptor IPR000276 346 372 PR00237 GPCR IPR004060 384 398 PR01522 Orexin receptor IPR004060 399 410 PR01522 Orexin receptor IPR004060 411 424 PR01522 Orexin receptor IPR000204 418 431 PR01064 Orexin receptor IPR004060 429 444 PR01522 Orexin receptor HMMPfam IPR000276 71 364 PF00001 GPCR IPR004060 384 444 PF03827 Orexin receptor ProfileScan IPR017452 71 364 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 56 VLIAGYIIVFVVALIGNVLVCVA 78 PRIMARY 23 2 92 FIVNLSLADVLVTITCLPATLVV 114 SECONDARY 23 3 127 CKVIPYLQTVSVSVSVLTLSCIA 149 PRIMARY 23 4 174 VIIWIVSCIIMIPQAIVMECSTV 196 PRIMARY 23 5 222 MYHICFFLVTYMAPLCLMVLAYL 244 PRIMARY 23 6 305 MLMIVLLVFAICYLPISILNVL 326 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001517 444 602393 100.0 100.0 XP_016866287 461 602393 100.0 100.0 XP_016856595 425 602392 69.2 92.3 XP_016856594 425 602392 69.2 92.3 NP_001516 425 602392 69.2 92.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7185 Download

>KIBB7185  1332 bp
ATGTCCGGCACCAAATTGGAGGACTCCCCCCCTTGTCGCAACTGGTCATCTGCTTCGGAG
CTGAATGAAACTCAAGAGCCCTTTTTAAACCCCACCGACTATGACGACGAGGAATTCCTG
CGGTACCTGTGGAGGGAATACCTGCACCCGAAAGAATATGAGTGGGTCCTGATCGCCGGG
TACATCATCGTGTTCGTCGTGGCTCTCATTGGGAACGTCCTGGTTTGTGTGGCAGTGTGG
AAGAACCACCACATGAGGACGGTAACCAACTACTTCATAGTCAATCTTTCTCTGGCTGAT
GTGCTCGTGACCATCACCTGCCTTCCAGCCACACTGGTCGTGGATATCACTGAGACCTGG
TTTTTTGGACAGTCCCTTTGCAAAGTGATTCCTTATCTACAGACCGTGTCGGTGTCTGTG
TCTGTCCTCACACTGAGCTGTATCGCCTTGGATCGGTGGTATGCAATCTGTCACCCTTTG
ATGTTTAAGAGCACAGCAAAGCGGGCCCGTAACAGCATTGTCATCATCTGGATTGTCTCC
TGCATTATAATGATTCCTCAGGCCATCGTCATGGAGTGCAGCACCGTGTTCCCAGGCTTA
GCCAATAAAACCACCCTCTTTACGGTGTGTGATGAGCGCTGGGGTGGTGAAATTTATCCC
AAGATGTACCACATCTGTTTCTTTCTGGTGACATACATGGCACCACTGTGTCTCATGGTG
TTGGCTTATCTGCAAATATTTCGCAAACTCTGGTGTCGACAGATCCCTGGAACATCATCT
GTAGTTCAGAGAAAATGGAAGCCCCTGCAGCCTGTTTCACAGCCTCGAGGGCCAGGACAG
CCAACGAAGTCCCGGATGAGCGCTGTGGCGGCTGAAATAAAGCAGATCCGAGCCAGAAGG
AAAACAGCCCGGATGTTGATGATTGTGCTTTTGGTATTTGCAATTTGCTATCTACCAATT
AGCATCCTCAATGTGCTAAAGAGAGTATTTGGGATGTTTGCCCATACTGAAGACAGAGAG
ACTGTGTATGCCTGGTTTACCTTTTCACACTGGCTTGTATATGCCAATAGTGCTGCGAAT
CCAATTATTTATAATTTTCTCAGTGGAAAATTTCGAGAGGAATTTAAAGCTGCGTTTTCT
TGCTGTTGCCTTGGAGTTCACCATCGCCAGGAGGATCGGCTCACCAGGGGACGAACTAGC
ACAGAGAGCCGGAAGTCCTTGACCACTCAAATCAGCAACTTTGATAACATATCAAAACTT
TCTGAGCAAGTTGTGCTCACTAGCATAAGCACACTCCCAGCAGCCAATGGAGCAGGACCA
CTTCAAAACTGG

ORF Protein

Cloned ORF protein sequence for pFN21AB7185 Download

>KIBB7185  444 aa
MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYEWVLIAG
YIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETW
FFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNSIVIIWIVS
CIIMIPQAIVMECSTVFPGLANKTTLFTVCDERWGGEIYPKMYHICFFLVTYMAPLCLMV
LAYLQIFRKLWCRQIPGTSSVVQRKWKPLQPVSQPRGPGQPTKSRMSAVAAEIKQIRARR
KTARMLMIVLLVFAICYLPISILNVLKRVFGMFAHTEDRETVYAWFTFSHWLVYANSAAN
PIIYNFLSGKFREEFKAAFSCCCLGVHHRQEDRLTRGRTSTESRKSLTTQISNFDNISKL
SEQVVLTSISTLPAANGAGPLQNW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7185 Download

>pFN21AB7185  6037 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCGGCACCAAATTGGAGGACTCCCCCCCTTG
TCGCAACTGGTCATCTGCTTCGGAGCTGAATGAAACTCAAGAGCCCTTTTTAAACCCCAC
CGACTATGACGACGAGGAATTCCTGCGGTACCTGTGGAGGGAATACCTGCACCCGAAAGA
ATATGAGTGGGTCCTGATCGCCGGGTACATCATCGTGTTCGTCGTGGCTCTCATTGGGAA
CGTCCTGGTTTGTGTGGCAGTGTGGAAGAACCACCACATGAGGACGGTAACCAACTACTT
CATAGTCAATCTTTCTCTGGCTGATGTGCTCGTGACCATCACCTGCCTTCCAGCCACACT
GGTCGTGGATATCACTGAGACCTGGTTTTTTGGACAGTCCCTTTGCAAAGTGATTCCTTA
TCTACAGACCGTGTCGGTGTCTGTGTCTGTCCTCACACTGAGCTGTATCGCCTTGGATCG
GTGGTATGCAATCTGTCACCCTTTGATGTTTAAGAGCACAGCAAAGCGGGCCCGTAACAG
CATTGTCATCATCTGGATTGTCTCCTGCATTATAATGATTCCTCAGGCCATCGTCATGGA
GTGCAGCACCGTGTTCCCAGGCTTAGCCAATAAAACCACCCTCTTTACGGTGTGTGATGA
GCGCTGGGGTGGTGAAATTTATCCCAAGATGTACCACATCTGTTTCTTTCTGGTGACATA
CATGGCACCACTGTGTCTCATGGTGTTGGCTTATCTGCAAATATTTCGCAAACTCTGGTG
TCGACAGATCCCTGGAACATCATCTGTAGTTCAGAGAAAATGGAAGCCCCTGCAGCCTGT
TTCACAGCCTCGAGGGCCAGGACAGCCAACGAAGTCCCGGATGAGCGCTGTGGCGGCTGA
AATAAAGCAGATCCGAGCCAGAAGGAAAACAGCCCGGATGTTGATGATTGTGCTTTTGGT
ATTTGCAATTTGCTATCTACCAATTAGCATCCTCAATGTGCTAAAGAGAGTATTTGGGAT
GTTTGCCCATACTGAAGACAGAGAGACTGTGTATGCCTGGTTTACCTTTTCACACTGGCT
TGTATATGCCAATAGTGCTGCGAATCCAATTATTTATAATTTTCTCAGTGGAAAATTTCG
AGAGGAATTTAAAGCTGCGTTTTCTTGCTGTTGCCTTGGAGTTCACCATCGCCAGGAGGA
TCGGCTCACCAGGGGACGAACTAGCACAGAGAGCCGGAAGTCCTTGACCACTCAAATCAG
CAACTTTGATAACATATCAAAACTTTCTGAGCAAGTTGTGCTCACTAGCATAAGCACACT
CCCAGCAGCCAATGGAGCAGGACCACTTCAAAACTGGGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT