SLC2A4 ORF clone FHC03812

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Halotag ORF Clone FHC03812

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Clone info.

Species

human

Clone Name

pFN21AB0463 Sequence (with vector) Restriction Map

Product ID

FHC03812

Flexi Clone PID

FXC03812

ORF Sequence

DNA sequence (1527 bp)
Protein sequence (509 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527885]

Gene

KIBB0463

Symbol

SLC2A4

Alias : GLUT4

Description

solute carrier family 2 (facilitated glucose transporter), member 4

Original Clone ID

cs01963

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1527 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 509 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11097.1 2.6e-207 100.0 SLC2A4 CCDS477.1 2.2e-132 65.5 SLC2A1 CCDS8586.1 1.2e-115 60.9 SLC2A3 CCDS66301.1 3.8e-112 60.0 SLC2A14 CCDS8585.1 6.1e-112 59.6 SLC2A14 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003663 35 45 PR00171 Sugar/inositol transporter IPR002441 54 62 PR01193 Glucose transporter IPR002441 64 76 PR01193 Glucose transporter IPR003663 139 158 PR00171 Sugar/inositol transporter IPR003663 298 308 PR00171 Sugar/inositol transporter IPR003663 385 406 PR00171 Sugar/inositol transporter IPR003663 408 420 PR00171 Sugar/inositol transporter IPR002441 486 498 PR01193 Glucose transporter IPR002441 499 509 PR01193 Glucose transporter HMMPfam IPR005828 29 483 PF00083 General substrate transporter HMMTigr IPR003663 21 479 TIGR00879 Sugar/inositol transporter ProfileScan IPR020846 27 472 PS50850 Major facilitator superfamily domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 19 RVTGTLVLAVFSAVLGSLQFGYN 41 PRIMARY 23 2 80 LWALSVAIFSVGGMISSFLIGII 102 PRIMARY 23 3 125 LMGLANAAASYEMLILGRFLIGA 147 SECONDARY 23 4 174 TLNQLAIVIGILIAQVLGLESLL 196 PRIMARY 23 5 203 PLLLGLTVLPALLQLVLLPFCPE 225 PRIMARY 23 6 288 LIIAVVLQLSQQLSGINAVFYYS 310 SECONDARY 23 7 325 ATIGAGVVNTVFTLVSVLLVERA 347 PRIMARY 23 8 352 LHLLGLAGMCGCAILMTVALLLL 374 PRIMARY 23 9 378 PAMSYVSIVAIFGFVAFFEIGPG 400 PRIMARY 23 10 419 AMAVAGFSNWTSNFIIGMGFQYV 441 SECONDARY 23 11 447 PYVFLLFAVLLLGFFIFTFLRVP 469 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001033 509 138190 100.0 100.0 NP_006507 492 138140,143090,608885 65.5 96.9 NP_008862 496 138170 60.9 91.6 XP_011518864 497 611039 60.0 91.7 XP_016874336 497 611039 60.0 91.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB0463 Download

>KIBB0463  1527 bp
ATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGATGGGGAACCCCCTCAGCAGCGAGTG
ACTGGGACCCTGGTCCTTGCTGTGTTCTCTGCGGTGCTTGGCTCCCTGCAGTTTGGGTAC
AACATTGGGGTCATCAATGCCCCTCAGAAGGTGATTGAACAGAGCTACAATGAGACGTGG
CTGGGGAGGCAGGGGCCTGAGGGACCCAGCTCCATCCCTCCAGGCACCCTCACCACCCTC
TGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGGCATGATTTCCTCCTTCCTCATTGGT
ATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCATGCTGGTCAACAATGTCCTGGCGGTG
CTGGGGGGCAGCCTCATGGGCCTGGCCAACGCTGCTGCCTCCTATGAAATGCTCATCCTT
GGACGATTCCTCATTGGCGCCTACTCAGGGCTGACATCAGGGCTGGTGCCCATGTACGTG
GGGGAGATTGCTCCCACTCACCTGCGGGGCGCCCTGGGGACGCTCAACCAACTGGCCATT
GTTATCGGCATTCTGATCGCCCAGGTGCTGGGCTTGGAGTCCCTCCTGGGCACTGCCAGC
CTGTGGCCACTGCTCCTGGGCCTCACAGTGCTACCTGCCCTCCTGCAGCTGGTCCTGCTG
CCCTTCTGTCCCGAGAGCCCCCGCTACCTCTACATCATCCAGAATCTCGAGGGGCCTGCC
AGAAAGAGTCTGAAGCGCCTGACAGGCTGGGCCGATGTTTCTGGAGTGCTGGCTGAGCTG
AAGGATGAGAAGCGGAAGCTGGAGCGTGAGCGGCCACTGTCCCTGCTCCAGCTCCTGGGC
AGCCGTACCCACCGGCAGCCCCTGATCATTGCGGTCGTGCTGCAGCTGAGCCAGCAGCTC
TCTGGCATCAATGCTGTTTTCTATTATTCGACCAGCATCTTCGAGACAGCAGGGGTAGGC
CAGCCTGCCTATGCCACCATAGGAGCTGGTGTGGTCAACACAGTCTTCACCTTGGTCTCG
GTGTTGTTGGTGGAGCGGGCGGGGCGCCGGACGCTCCATCTCCTGGGCCTGGCGGGCATG
TGTGGCTGTGCCATCCTGATGACTGTGGCTCTGCTCCTGCTGGAGCGAGTTCCAGCCATG
AGCTACGTCTCCATTGTGGCCATCTTTGGCTTCGTGGCATTTTTTGAGATTGGCCCTGGC
CCCATTCCTTGGTTCATCGTGGCCGAGCTCTTCAGCCAGGGACCCCGCCCGGCAGCCATG
GCTGTGGCTGGTTTCTCCAACTGGACGAGCAACTTCATCATTGGCATGGGTTTCCAGTAT
GTTGCGGAGGCTATGGGGCCCTACGTCTTCCTTCTATTTGCGGTCCTCCTGCTGGGCTTC
TTCATCTTCACCTTCTTAAGAGTACCTGAAACTCGAGGCCGGACGTTTGACCAGATCTCA
GCTGCCTTCCACCGGACACCCTCTCTTTTAGAGCAGGAGGTGAAACCCAGCACAGAACTT
GAGTATTTAGGGCCAGATGAGAACGAC

ORF Protein

Cloned ORF protein sequence for pFN21AB0463 Download

>KIBB0463  509 aa
MPSGFQQIGSEDGEPPQQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNETW
LGRQGPEGPSSIPPGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLVNNVLAV
LGGSLMGLANAAASYEMLILGRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAI
VIGILIAQVLGLESLLGTASLWPLLLGLTVLPALLQLVLLPFCPESPRYLYIIQNLEGPA
RKSLKRLTGWADVSGVLAELKDEKRKLERERPLSLLQLLGSRTHRQPLIIAVVLQLSQQL
SGINAVFYYSTSIFETAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRRTLHLLGLAGM
CGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAM
AVAGFSNWTSNFIIGMGFQYVAEAMGPYVFLLFAVLLLGFFIFTFLRVPETRGRTFDQIS
AAFHRTPSLLEQEVKPSTELEYLGPDEND

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB0463 Download

>pFN21AB0463  6232 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGA
TGGGGAACCCCCTCAGCAGCGAGTGACTGGGACCCTGGTCCTTGCTGTGTTCTCTGCGGT
GCTTGGCTCCCTGCAGTTTGGGTACAACATTGGGGTCATCAATGCCCCTCAGAAGGTGAT
TGAACAGAGCTACAATGAGACGTGGCTGGGGAGGCAGGGGCCTGAGGGACCCAGCTCCAT
CCCTCCAGGCACCCTCACCACCCTCTGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGG
CATGATTTCCTCCTTCCTCATTGGTATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCAT
GCTGGTCAACAATGTCCTGGCGGTGCTGGGGGGCAGCCTCATGGGCCTGGCCAACGCTGC
TGCCTCCTATGAAATGCTCATCCTTGGACGATTCCTCATTGGCGCCTACTCAGGGCTGAC
ATCAGGGCTGGTGCCCATGTACGTGGGGGAGATTGCTCCCACTCACCTGCGGGGCGCCCT
GGGGACGCTCAACCAACTGGCCATTGTTATCGGCATTCTGATCGCCCAGGTGCTGGGCTT
GGAGTCCCTCCTGGGCACTGCCAGCCTGTGGCCACTGCTCCTGGGCCTCACAGTGCTACC
TGCCCTCCTGCAGCTGGTCCTGCTGCCCTTCTGTCCCGAGAGCCCCCGCTACCTCTACAT
CATCCAGAATCTCGAGGGGCCTGCCAGAAAGAGTCTGAAGCGCCTGACAGGCTGGGCCGA
TGTTTCTGGAGTGCTGGCTGAGCTGAAGGATGAGAAGCGGAAGCTGGAGCGTGAGCGGCC
ACTGTCCCTGCTCCAGCTCCTGGGCAGCCGTACCCACCGGCAGCCCCTGATCATTGCGGT
CGTGCTGCAGCTGAGCCAGCAGCTCTCTGGCATCAATGCTGTTTTCTATTATTCGACCAG
CATCTTCGAGACAGCAGGGGTAGGCCAGCCTGCCTATGCCACCATAGGAGCTGGTGTGGT
CAACACAGTCTTCACCTTGGTCTCGGTGTTGTTGGTGGAGCGGGCGGGGCGCCGGACGCT
CCATCTCCTGGGCCTGGCGGGCATGTGTGGCTGTGCCATCCTGATGACTGTGGCTCTGCT
CCTGCTGGAGCGAGTTCCAGCCATGAGCTACGTCTCCATTGTGGCCATCTTTGGCTTCGT
GGCATTTTTTGAGATTGGCCCTGGCCCCATTCCTTGGTTCATCGTGGCCGAGCTCTTCAG
CCAGGGACCCCGCCCGGCAGCCATGGCTGTGGCTGGTTTCTCCAACTGGACGAGCAACTT
CATCATTGGCATGGGTTTCCAGTATGTTGCGGAGGCTATGGGGCCCTACGTCTTCCTTCT
ATTTGCGGTCCTCCTGCTGGGCTTCTTCATCTTCACCTTCTTAAGAGTACCTGAAACTCG
AGGCCGGACGTTTGACCAGATCTCAGCTGCCTTCCACCGGACACCCTCTCTTTTAGAGCA
GGAGGTGAAACCCAGCACAGAACTTGAGTATTTAGGGCCAGATGAGAACGACGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT