PPP1R15A ORF clone FHC06456

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC06456

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Clone info.

Species

human

Clone Name

pFN21AB7064 Sequence (with vector) Restriction Map

Product ID

FHC06456

Flexi Clone PID

FXC06456

ORF Sequence

DNA sequence (2022 bp)
Protein sequence (674 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464097]

Gene

KIBB7064

Symbol

PPP1R15A

Alias : GADD34

Description

protein phosphatase 1, regulatory subunit 15A

Original Clone ID

cp01069

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2022 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 674 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12738.1 2e-160 100.0 PPP1R15A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019523 544 619 PF10488 Protein phosphatase 1
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055145 674 611048 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7064 Download

>KIBB7064  2022 bp
ATGGCCCCAGGCCAAGCACCCCATCAGGCTACCCCGTGGAGGGATGCCCACCCTTTCTTC
CTCCTGTCCCCAGTGATGGGCCTCCTCAGCCGCGCCTGGAGCCGCCTGAGGGGCCTGGGA
CCTCTAGAGCCCTGGCTGGTGGAAGCAGTAAAAGGAGCAGCTCTGGTAGAAGCTGGCCTG
GAGGGAGAAGCTAGGACTCCTCTGGCAATCCCCCATACCCCTTGGGGCAGACGCCCTGAA
GAGGAGGCTGAAGACAGTGGAGGCCCTGGAGAGGACAGAGAAACACTGGGGCTGAAAACC
AGCAGTTCCCTTCCTGAAGCCTGGGGACTTTTGGATGATGATGATGGCATGTATGGTGAG
CGAGAGGCAACCAGTGTCCCTAGAGGGCAGGGAAGTCAATTTGCAGATGGCCAGCGTGCT
CCCCTGTCTCCCAGCCTTCTGATAAGGACACTGCAAGGTTCTGATAAGAACCCAGGGGAG
GAGAAAGCCGAGGAAGAGGGAGTTGCTGAAGAGGAGGGAGTTAACAAGTTCTCTTATCCA
CCATCACACCGGGAGTGTTGTCCAGCCGTGGAGGAGGAGGACGATGAAGAAGCTGTAAAG
AAAGAAGCTCACAGAACCTCTACTTCTGCCTTGTCTCCAGGATCCAAGCCCAGCACTTGG
GTGTCTTGCCCAGGGGAGGAAGAGAATCAAGCCACGGAGGATAAAAGAACAGAAAGAAGT
AAAGGAGCCAGGAAGACCTCCGTGTCCCCCCGATCTTCAGGCTCCGACCCCAGGTCCTGG
GAGTATCGTTCAGGAGAGGCGTCCGAGGAGAAGGAGGAAAAGGCACACAAAGAAACTGGG
AAAGGAGAAGCTGCCCCAGGGCCGCAATCCTCAGCCCCAGCCCAGAGGCCCCAGCTCAAG
TCCTGGTGGTGCCAACCCAGTGATGAAGAGGAGGGTGAGGTCAAGGCTTTGGGGGCAGCT
GAGAAGGATGGAGAAGCTGAGTGTCCTCCCTGCATCCCCCCACCAAGTGCCTTCCTGAAG
GCCTGGGTGTATTGGCCAGGAGAGGACACAGAGGAAGAGGAAGATGAGGAAGAAGATGAG
GACAGTGACTCTGGATCAGATGAGGAAGAGGGAGAAGCTGAGGCTTCCTCTTCCACTCCT
GCTACAGGTGTCTTCTTGAAGTCCTGGGTCTATCAGCCAGGAGAGGACACAGAGGAGGAG
GAAGATGAGGACAGTGATACAGGATCAGCCGAGGATGAAAGAGAAGCTGAGACTTCTGCT
TCCACACCCCCTGCAAGTGCTTTCTTGAAGGCCTGGGTGTATCGGCCAGGAGAGGACACG
GAGGAGGAGGAAGATGAGGATGTGGATAGTGAGGATAAGGAAGATGATTCAGAAGCAGCC
TTGGGAGAAGCTGAGTCAGACCCACATCCCTCCCACCCGGACCAGAGGGCCCACTTCAGG
GGCTGGGGATATCGACCTGGAAAAGAGACAGAGGAAGAGGAAGCTGCTGAGGACTGGGGA
GAAGCTGAGCCCTGCCCCTTCCGAGTGGCCATCTATGTACCTGGAGAGAAGCCACCGCCT
CCCTGGGCTCCTCCTAGGCTGCCCCTCCGACTGCAAAGGCGGCTCAAGCGCCCAGAAACC
CCTACTCATGATCCGGACCCTGAGACTCCCCTAAAGGCCAGAAAGGTGCGCTTCTCCGAG
AAGGTCACTGTCCATTTCCTGGCTGTCTGGGCAGGGCCGGCCCAGGCCGCCCGCCAGGGC
CCCTGGGAGCAGCTTGCTCGGGATCGCAGCCGCTTCGCACGCCGCATCACCCAGGCCCAG
GAGGAGCTGAGCCCCTGCCTCACCCCTGCTGCCCGGGCCAGAGCCTGGGCACGCCTCAGG
AACCCACCTTTAGCCCCCATCCCTGCCCTCACCCAGACCTTGCCTTCCTCCTCTGTCCCT
TCGTCCCCAGTCCAGACCACGCCCTTGAGCCAAGCTGTGGCCACACCTTCCCGCTCGTCT
GCTGCTGCAGCGGCTGCCCTGGACCTCAGTGGGAGGCGTGGC

ORF Protein

Cloned ORF protein sequence for pFN21AB7064 Download

>KIBB7064  674 aa
MAPGQAPHQATPWRDAHPFFLLSPVMGLLSRAWSRLRGLGPLEPWLVEAVKGAALVEAGL
EGEARTPLAIPHTPWGRRPEEEAEDSGGPGEDRETLGLKTSSSLPEAWGLLDDDDGMYGE
REATSVPRGQGSQFADGQRAPLSPSLLIRTLQGSDKNPGEEKAEEEGVAEEEGVNKFSYP
PSHRECCPAVEEEDDEEAVKKEAHRTSTSALSPGSKPSTWVSCPGEEENQATEDKRTERS
KGARKTSVSPRSSGSDPRSWEYRSGEASEEKEEKAHKETGKGEAAPGPQSSAPAQRPQLK
SWWCQPSDEEEGEVKALGAAEKDGEAECPPCIPPPSAFLKAWVYWPGEDTEEEEDEEEDE
DSDSGSDEEEGEAEASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSA
STPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFR
GWGYRPGKETEEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPET
PTHDPDPETPLKARKVRFSEKVTVHFLAVWAGPAQAARQGPWEQLARDRSRFARRITQAQ
EELSPCLTPAARARAWARLRNPPLAPIPALTQTLPSSSVPSSPVQTTPLSQAVATPSRSS
AAAAAALDLSGRRG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7064 Download

>pFN21AB7064  6727 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCCCAGGCCAAGCACCCCATCAGGCTACCCC
GTGGAGGGATGCCCACCCTTTCTTCCTCCTGTCCCCAGTGATGGGCCTCCTCAGCCGCGC
CTGGAGCCGCCTGAGGGGCCTGGGACCTCTAGAGCCCTGGCTGGTGGAAGCAGTAAAAGG
AGCAGCTCTGGTAGAAGCTGGCCTGGAGGGAGAAGCTAGGACTCCTCTGGCAATCCCCCA
TACCCCTTGGGGCAGACGCCCTGAAGAGGAGGCTGAAGACAGTGGAGGCCCTGGAGAGGA
CAGAGAAACACTGGGGCTGAAAACCAGCAGTTCCCTTCCTGAAGCCTGGGGACTTTTGGA
TGATGATGATGGCATGTATGGTGAGCGAGAGGCAACCAGTGTCCCTAGAGGGCAGGGAAG
TCAATTTGCAGATGGCCAGCGTGCTCCCCTGTCTCCCAGCCTTCTGATAAGGACACTGCA
AGGTTCTGATAAGAACCCAGGGGAGGAGAAAGCCGAGGAAGAGGGAGTTGCTGAAGAGGA
GGGAGTTAACAAGTTCTCTTATCCACCATCACACCGGGAGTGTTGTCCAGCCGTGGAGGA
GGAGGACGATGAAGAAGCTGTAAAGAAAGAAGCTCACAGAACCTCTACTTCTGCCTTGTC
TCCAGGATCCAAGCCCAGCACTTGGGTGTCTTGCCCAGGGGAGGAAGAGAATCAAGCCAC
GGAGGATAAAAGAACAGAAAGAAGTAAAGGAGCCAGGAAGACCTCCGTGTCCCCCCGATC
TTCAGGCTCCGACCCCAGGTCCTGGGAGTATCGTTCAGGAGAGGCGTCCGAGGAGAAGGA
GGAAAAGGCACACAAAGAAACTGGGAAAGGAGAAGCTGCCCCAGGGCCGCAATCCTCAGC
CCCAGCCCAGAGGCCCCAGCTCAAGTCCTGGTGGTGCCAACCCAGTGATGAAGAGGAGGG
TGAGGTCAAGGCTTTGGGGGCAGCTGAGAAGGATGGAGAAGCTGAGTGTCCTCCCTGCAT
CCCCCCACCAAGTGCCTTCCTGAAGGCCTGGGTGTATTGGCCAGGAGAGGACACAGAGGA
AGAGGAAGATGAGGAAGAAGATGAGGACAGTGACTCTGGATCAGATGAGGAAGAGGGAGA
AGCTGAGGCTTCCTCTTCCACTCCTGCTACAGGTGTCTTCTTGAAGTCCTGGGTCTATCA
GCCAGGAGAGGACACAGAGGAGGAGGAAGATGAGGACAGTGATACAGGATCAGCCGAGGA
TGAAAGAGAAGCTGAGACTTCTGCTTCCACACCCCCTGCAAGTGCTTTCTTGAAGGCCTG
GGTGTATCGGCCAGGAGAGGACACGGAGGAGGAGGAAGATGAGGATGTGGATAGTGAGGA
TAAGGAAGATGATTCAGAAGCAGCCTTGGGAGAAGCTGAGTCAGACCCACATCCCTCCCA
CCCGGACCAGAGGGCCCACTTCAGGGGCTGGGGATATCGACCTGGAAAAGAGACAGAGGA
AGAGGAAGCTGCTGAGGACTGGGGAGAAGCTGAGCCCTGCCCCTTCCGAGTGGCCATCTA
TGTACCTGGAGAGAAGCCACCGCCTCCCTGGGCTCCTCCTAGGCTGCCCCTCCGACTGCA
AAGGCGGCTCAAGCGCCCAGAAACCCCTACTCATGATCCGGACCCTGAGACTCCCCTAAA
GGCCAGAAAGGTGCGCTTCTCCGAGAAGGTCACTGTCCATTTCCTGGCTGTCTGGGCAGG
GCCGGCCCAGGCCGCCCGCCAGGGCCCCTGGGAGCAGCTTGCTCGGGATCGCAGCCGCTT
CGCACGCCGCATCACCCAGGCCCAGGAGGAGCTGAGCCCCTGCCTCACCCCTGCTGCCCG
GGCCAGAGCCTGGGCACGCCTCAGGAACCCACCTTTAGCCCCCATCCCTGCCCTCACCCA
GACCTTGCCTTCCTCCTCTGTCCCTTCGTCCCCAGTCCAGACCACGCCCTTGAGCCAAGC
TGTGGCCACACCTTCCCGCTCGTCTGCTGCTGCAGCGGCTGCCCTGGACCTCAGTGGGAG
GCGTGGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT