MAPK15 ORF clone pF1KE0975

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Flexi ORF Clone pF1KE0975

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Clone info.

Species

human

Clone Name

pF1KE0975 Sequence (with vector) Restriction Map

Product ID

FXC10717

HaloTag Clone PID

FHC10717

ORF Sequence

DNA sequence (1632 bp)
Protein sequence (544 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI
Because the PmeI site of this clone is affected by EcoKI methylase, we strongly recommend use of EcoKI methylase-free E.coli strain for preparation of this clone.

GenBank Accession

ORF sequence is derived from [AB587543]

Gene

KIEE0975

Symbol

MAPK15

Alias : ERK7, ERK8

Description

mitogen-activated protein kinase 15

Original Clone ID

cp01475

Features of the cloned ORF sequence

Description

Length: 1632 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 544 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6409.2 1.2e-104 100.0 MAPK15 CCDS42149.1 1.6e-20 45.2 MAPK3 CCDS10672.1 1.7e-20 45.2 MAPK3 CCDS11206.1 2.2e-20 37.3 MAPK7 CCDS13795.1 5.9e-20 44.7 MAPK1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017442 15 304 PF00069 Serine/threonine-protein kinase-like domain HMMSmart IPR002290 13 304 SM00220 Serine/threonine-protein kinase domain IPR020635 13 307 SM00219 Tyrosine-protein kinase ProfileScan IPR000719 13 304 PS50011 Protein kinase Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE0975 Download

>KIEE0975  1632 bp
ATGTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAGCTCGGG
CAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAGGTCGTGGCC
ATCAAGAAAATCTTTGATGCTTTTAGGGATAAGACAGATGCCCAGAGAACATTCCGGGAA
ATCACGCTCCTCCAGGAGTTTGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATC
CGGGCAGAGAACGACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTGAAC
GCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTCCATCTTCTACCAG
CTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACGTTGTGCACCGGGACCAGAAGCCG
TCCAATGTGCTCCTGGATGCCAACTGCACAGTGAAGCTGTGTGACTTTGGCCTGGCCCGC
TCCCTGGGCGACCTCCCTGAGGGGCCTGAGGACCAGGCCGTGACAGAGTACGTGGCCACA
CGCTGGTACCGAGCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTGGAC
ATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACCCCTGTTCCCCGGC
ACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGACCATCCCACCGCCATCTGAGGAG
GACCTCCTGGCTCTCGGCTCAGGCTGCCGTGCCTCTGTGCTGCACCAGCTGGGGTCCCGG
CCACGACAGACGCTGGATGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTC
CTTAGGCGACTCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTGCAG
CACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACGAGAGGCAGATGTG
CGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGCCTGAGTACCGCAGCCGCGTCTAT
CAGATGATCCTGGAGTGTGGAGGCAGCAGCGGCACCTCGAGAGAGAAGGGCCCGGAGGGT
GTCTCCCCAAGCCAGGCACACCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGG
ACACCTGTGCAGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCCGAG
CACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGCTCCCCTGCTCCAA
ACTGCTCTCCTAGGGAATGGGGAAAGGCCCCCTGGGGCGAAGGAAGCGCCCCCCTTGACA
CTCTCGCTGGTGAAGCCAAGCGGGAGGGGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCG
GCTCAGGTGGCCAACCAGGCCCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGG
GTGGCCAGCGTACAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGGAGG
ATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGCTTTGCTTGGAGGC
TACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCACTGGGCCACCTGCCCCTGCTGGAG
GGGCACCATGTG

ORF Protein

Cloned ORF protein sequence for pF1KE0975 Download

>KIEE0975  544 aa
MCTVVDPRIVRRYLLRRQLGQGAYGIVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFRE
ITLLQEFGDHPNIISLLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQ
LLRATRFLHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVTEYVAT
RWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLHQLELILETIPPPSEE
DLLALGSGCRASVLHQLGSRPRQTLDALLPPDTSPEALDLLRRLLVFAPDKRLSATQALQ
HPYVQRFHCPSDEWAREADVRPRAHEGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEG
VSPSQAHLHKPRADPQLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQ
TALLGNGERPPGAKEAPPLTLSLVKPSGRGAAPSLTSQAAAQVANQALIRGDWNRGGGVR
VASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGGYSQAYGTVCHSALGHLPLLE
GHHV

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE0975 Download

>pF1KE0975  4745 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGTGCACCGTA
GTGGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAGCTCGGGCAGGGGGCCTAT
GGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATC
TTTGATGCTTTTAGGGATAAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTC
CAGGAGTTTGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAGAGAAC
GACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTGAACGCAGTCATCCGG
AAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTCCATCTTCTACCAGCTCCTGCGGGCC
ACCCGGTTCCTCCACTCGGGGCACGTTGTGCACCGGGACCAGAAGCCGTCCAATGTGCTC
CTGGATGCCAACTGCACAGTGAAGCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCGAC
CTCCCTGAGGGGCCTGAGGACCAGGCCGTGACAGAGTACGTGGCCACACGCTGGTACCGA
GCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTGGACATGTGGAGTCTG
GGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACCCCTGTTCCCCGGCACGTCCACCCTC
CACCAGCTGGAGCTGATCCTGGAGACCATCCCACCGCCATCTGAGGAGGACCTCCTGGCT
CTCGGCTCAGGCTGCCGTGCCTCTGTGCTGCACCAGCTGGGGTCCCGGCCACGACAGACG
CTGGATGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTCCTTAGGCGACTC
CTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTGCAGCACCCCTACGTG
CAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACGAGAGGCAGATGTGCGGCCCCGGGCA
CACGAAGGGGTCCAGCTCTCTGTGCCTGAGTACCGCAGCCGCGTCTATCAGATGATCCTG
GAGTGTGGAGGCAGCAGCGGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGC
CAGGCACACCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGGACACCTGTGCAG
GGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCCGAGCACGAGTCCCCC
CGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGCTCCCCTGCTCCAAACTGCTCTCCTA
GGGAATGGGGAAAGGCCCCCTGGGGCGAAGGAAGCGCCCCCCTTGACACTCTCGCTGGTG
AAGCCAAGCGGGAGGGGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCC
AACCAGGCCCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCAGCGTA
CAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGGAGGATGTTCAGCACC
TCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGCTTTGCTTGGAGGCTACTCCCAAGCC
TACGGGACTGTCTGCCACTCGGCACTGGGCCACCTGCCCCTGCTGGAGGGGCACCATGTG
GTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA
AGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGA
GCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA
AGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCA
TGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGAT
AGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGT
GTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAA
GAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATG
GCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGA
TGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGG
GTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCC
GTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGT
GCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTT
CCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGC
GAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATC
ATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCAC
CAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAG
GATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAG
GCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAAT
ATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCG
GACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAA
TGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCC
TTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACC
AAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC
AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA
AAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAA
TCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCC
CCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTC
CGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAG
TTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGA
CCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATC
GCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTAC
AGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTG
CGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACA
AACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAA
AGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAA
CTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTT
ATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATG
AAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCAT
AGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGT
TGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAA
TTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCA
GTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGAT
TAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGT
GGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT
GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGT
CGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGA
CAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAG
GACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCC
TTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCAT