CHRNG ORF clone pF1KE2392

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Flexi ORF Clone pF1KE2392

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Clone info.

Species

human

Clone Name

pF1KE2392 Sequence (with vector) Restriction Map

Product ID

FXC28786

HaloTag Clone PID

ORF Sequence

DNA sequence (1551 bp)
Protein sequence (517 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2392

Symbol

CHRNG

Alias : ACHRG

Description

cholinergic receptor, nicotinic, gamma (muscle)

Original Clone ID

cp01554

Features of the cloned ORF sequence

Description

Length: 1551 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 517 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33400.1 0 100.0 CHRNG CCDS2494.1 1.9e-99 48.0 CHRND CCDS11058.1 7.1e-90 50.7 CHRNE CCDS58754.1 8.4e-85 45.9 CHRND CCDS11106.1 7.4e-82 42.5 CHRNB1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002394 60 76 PR00254 Nicotinic acetylcholine receptor IPR006201 73 89 PR00252 Neurotransmitter-gated ion-channel IPR002394 94 108 PR00254 Nicotinic acetylcholine receptor IPR006201 106 117 PR00252 Neurotransmitter-gated ion-channel IPR002394 112 124 PR00254 Nicotinic acetylcholine receptor IPR002394 130 148 PR00254 Nicotinic acetylcholine receptor IPR006201 150 164 PR00252 Neurotransmitter-gated ion-channel IPR006201 235 247 PR00252 Neurotransmitter-gated ion-channel HMMPfam IPR006202 26 241 PF02931 Neurotransmitter-gated ion-channel ligand-binding IPR006029 248 492 PF02932 Neurotransmitter-gated ion-channel transmembrane domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 5 QGPLLLLLLLAVCLGA 20 PRIMARY 16 2 245 VINIIAPCVLISSVAILIHFLPA 267 PRIMARY 23 3 273 KCTVAINVLLAQTVFLFLVA 292 PRIMARY 20 4 305 ISKYLTFLLVVTILIVVNAVVVL 327 PRIMARY 23 5 470 RVLDRVCFLAMLSLFICGTAGIF 492 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005190 517 100730,253290,265000 100.0 100.0 NP_000742 517 100720,253290,616321,616322,616323 48.0 98.3 NP_000071 493 100725,605809,608931,616324 50.7 98.1 XP_016879604 481 100725,605809,608931,616324 51.2 94.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE2392 Download

>KIEE2392  1551 bp
ATGCATGGGGGCCAGGGGCCGCTGCTCCTCCTGCTGCTGCTGGCTGTCTGCCTGGGGGCC
CAGGGCCGGAACCAGGAGGAGCGCCTGCTCGCAGACCTGATGCAAAACTACGACCCCAAC
CTGCGGCCCGCGGAACGAGACTCGGATGTGGTCAATGTCAGCCTGAAGCTAACCCTCACC
AACCTCATCTCCCTGAACGAGCGAGAGGAAGCCCTCACCACCAATGTCTGGATAGAGATG
CAGTGGTGCGACTATCGCCTGCGCTGGGATCCGCGAGACTACGAAGGCCTGTGGGTGCTG
AGGGTGCCGTCCACCATGGTGTGGCGGCCGGATATCGTGCTGGAGAACAACGTGGACGGT
GTCTTCGAGGTGGCCCTCTACTGCAATGTGCTCGTGTCCCCTGACGGCTGTATCTACTGG
CTGCCGCCTGCCATCTTCCGTTCCGCCTGCTCTATCTCAGTCACCTACTTCCCCTTCGAC
TGGCAGAACTGCTCCCTTATCTTCCAGTCCCAGACTTACAGCACCAATGAGATTGATCTG
CAGCTGAGTCAGGAAGATGGCCAGACCATCGAGTGGATTTTCATTGACCCTGAGGCCTTC
ACAGAGAATGGGGAGTGGGCCATCCAGCACCGACCAGCCAAGATGCTCCTGGACCCAGCG
GCGCCAGCCCAGGAAGCAGGCCACCAGAAGGTGGTGTTCTACCTGCTCATCCAGCGCAAG
CCCCTCTTCTACGTCATCAACATCATCGCCCCCTGTGTGCTCATCTCCTCTGTCGCCATC
CTCATCCACTTCCTTCCTGCCAAGGCTGGGGGCCAGAAGTGTACCGTCGCCATCAACGTG
CTCCTGGCCCAGACTGTCTTCCTCTTCCTTGTGGCCAAGAAGGTGCCTGAAACCTCCCAG
GCGGTGCCACTCATCAGCAAGTACCTGACCTTCCTCCTGGTGGTGACCATCCTCATTGTC
GTGAATGCTGTGGTTGTGCTCAATGTCTCCTTGCGGTCTCCACACACACACTCCATGGCC
CGAGGGGTCCGCAAGGTGTTCCTGAGGCTCTTGCCCCAGCTGCTGAGGATGCACGTTCGC
CCGCTGGCCCCGGCAGCTGTGCAGGACACCCAGTCCCGGCTACAGAATGGCTCCTCGGGA
TGGTCGATCACAACTGGGGAGGAGGTGGCCCTCTGCCTGCCTCGCAGTGAACTCCTCTTC
CAGCAGTGGCAGCGGCAAGGGCTGGTGGCGGCAGCGCTGGAGAAGCTAGAGAAAGGCCCG
GAGTTAGGGCTGAGCCAGTTCTGTGGCAGCCTGAAGCAGGCTGCCCCAGCCATCCAGGCC
TGTGTGGAAGCCTGCAACCTCATTGCCTGTGCCCGGCACCAGCAGAGTCACTTTGACAAT
GGGAATGAGGAGTGGTTCCTGGTGGGCCGAGTGCTGGACCGTGTCTGCTTCCTGGCCATG
CTCTCGCTCTTCATCTGTGGCACAGCTGGCATCTTCCTCATGGCCCACTACAACCGGGTG
CCGGCCCTGCCATTCCCTGGAGATCCACGCCCCTACCTGCCCTCACCAGAC

ORF Protein

Cloned ORF protein sequence for pF1KE2392 Download

>KIEE2392  517 aa
MHGGQGPLLLLLLLAVCLGAQGRNQEERLLADLMQNYDPNLRPAERDSDVVNVSLKLTLT
NLISLNEREEALTTNVWIEMQWCDYRLRWDPRDYEGLWVLRVPSTMVWRPDIVLENNVDG
VFEVALYCNVLVSPDGCIYWLPPAIFRSACSISVTYFPFDWQNCSLIFQSQTYSTNEIDL
QLSQEDGQTIEWIFIDPEAFTENGEWAIQHRPAKMLLDPAAPAQEAGHQKVVFYLLIQRK
PLFYVINIIAPCVLISSVAILIHFLPAKAGGQKCTVAINVLLAQTVFLFLVAKKVPETSQ
AVPLISKYLTFLLVVTILIVVNAVVVLNVSLRSPHTHSMARGVRKVFLRLLPQLLRMHVR
PLAPAAVQDTQSRLQNGSSGWSITTGEEVALCLPRSELLFQQWQRQGLVAAALEKLEKGP
ELGLSQFCGSLKQAAPAIQACVEACNLIACARHQQSHFDNGNEEWFLVGRVLDRVCFLAM
LSLFICGTAGIFLMAHYNRVPALPFPGDPRPYLPSPD

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE2392 Download

>pF1KE2392  4664 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGCATGGGGGC
CAGGGGCCGCTGCTCCTCCTGCTGCTGCTGGCTGTCTGCCTGGGGGCCCAGGGCCGGAAC
CAGGAGGAGCGCCTGCTCGCAGACCTGATGCAAAACTACGACCCCAACCTGCGGCCCGCG
GAACGAGACTCGGATGTGGTCAATGTCAGCCTGAAGCTAACCCTCACCAACCTCATCTCC
CTGAACGAGCGAGAGGAAGCCCTCACCACCAATGTCTGGATAGAGATGCAGTGGTGCGAC
TATCGCCTGCGCTGGGATCCGCGAGACTACGAAGGCCTGTGGGTGCTGAGGGTGCCGTCC
ACCATGGTGTGGCGGCCGGATATCGTGCTGGAGAACAACGTGGACGGTGTCTTCGAGGTG
GCCCTCTACTGCAATGTGCTCGTGTCCCCTGACGGCTGTATCTACTGGCTGCCGCCTGCC
ATCTTCCGTTCCGCCTGCTCTATCTCAGTCACCTACTTCCCCTTCGACTGGCAGAACTGC
TCCCTTATCTTCCAGTCCCAGACTTACAGCACCAATGAGATTGATCTGCAGCTGAGTCAG
GAAGATGGCCAGACCATCGAGTGGATTTTCATTGACCCTGAGGCCTTCACAGAGAATGGG
GAGTGGGCCATCCAGCACCGACCAGCCAAGATGCTCCTGGACCCAGCGGCGCCAGCCCAG
GAAGCAGGCCACCAGAAGGTGGTGTTCTACCTGCTCATCCAGCGCAAGCCCCTCTTCTAC
GTCATCAACATCATCGCCCCCTGTGTGCTCATCTCCTCTGTCGCCATCCTCATCCACTTC
CTTCCTGCCAAGGCTGGGGGCCAGAAGTGTACCGTCGCCATCAACGTGCTCCTGGCCCAG
ACTGTCTTCCTCTTCCTTGTGGCCAAGAAGGTGCCTGAAACCTCCCAGGCGGTGCCACTC
ATCAGCAAGTACCTGACCTTCCTCCTGGTGGTGACCATCCTCATTGTCGTGAATGCTGTG
GTTGTGCTCAATGTCTCCTTGCGGTCTCCACACACACACTCCATGGCCCGAGGGGTCCGC
AAGGTGTTCCTGAGGCTCTTGCCCCAGCTGCTGAGGATGCACGTTCGCCCGCTGGCCCCG
GCAGCTGTGCAGGACACCCAGTCCCGGCTACAGAATGGCTCCTCGGGATGGTCGATCACA
ACTGGGGAGGAGGTGGCCCTCTGCCTGCCTCGCAGTGAACTCCTCTTCCAGCAGTGGCAG
CGGCAAGGGCTGGTGGCGGCAGCGCTGGAGAAGCTAGAGAAAGGCCCGGAGTTAGGGCTG
AGCCAGTTCTGTGGCAGCCTGAAGCAGGCTGCCCCAGCCATCCAGGCCTGTGTGGAAGCC
TGCAACCTCATTGCCTGTGCCCGGCACCAGCAGAGTCACTTTGACAATGGGAATGAGGAG
TGGTTCCTGGTGGGCCGAGTGCTGGACCGTGTCTGCTTCCTGGCCATGCTCTCGCTCTTC
ATCTGTGGCACAGCTGGCATCTTCCTCATGGCCCACTACAACCGGGTGCCGGCCCTGCCA
TTCCCTGGAGATCCACGCCCCTACCTGCCCTCACCAGACGTTTAAACGAATTCGAGCTCG
GTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAA
AGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCT
TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTC
GCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTAC
CTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCAT
CCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCC
CTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCC
TCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATC
TGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTT
GAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTAT
GACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAG
GGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGAC
GAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGAC
GTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTC
CTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGG
CTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAG
CGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCAT
CAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAG
GATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGC
TTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCG
TTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTG
CTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAG
TTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATC
AGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAA
CATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTT
TTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTG
GCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCG
CTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAG
CGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTC
CAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAA
CTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG
TAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCC
TAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTAC
CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGG
TTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTT
GATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGT
CATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAAC
GCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGA
TTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAAT
TTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCT
GCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCC
ACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTC
CGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGG
TCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCC
GAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGT
AGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTT
TTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATT
TGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCA
GGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTC
TTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT