ME3 ORF clone pF1KE2477

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Flexi ORF Clone pF1KE2477

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Clone info.

Species

human

Clone Name

pF1KE2477 Sequence (with vector) Restriction Map

Product ID

FXC10155

HaloTag Clone PID

ORF Sequence

DNA sequence (1812 bp)
Protein sequence (604 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2477

Symbol

ME3

Alias : NADP-ME

Description

malic enzyme 3, NADP(+)-dependent, mitochondrial, transcript variant 1

Original Clone ID

oc01534

Features of the cloned ORF sequence

Description

Length: 1812 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 604 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8277.1 0 99.5 ME3 CCDS34492.1 8.7e-174 71.3 ME1 CCDS11948.1 2.2e-133 55.9 ME2 CCDS54187.1 8.5e-113 57.6 ME2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001891 120 144 PR00072 Malic oxidoreductase IPR001891 180 209 PR00072 Malic oxidoreductase IPR001891 216 238 PR00072 Malic oxidoreductase IPR001891 276 294 PR00072 Malic oxidoreductase IPR001891 301 317 PR00072 Malic oxidoreductase IPR001891 332 348 PR00072 Malic oxidoreductase IPR001891 433 449 PR00072 Malic oxidoreductase HMMPfam IPR012301 114 295 PF00390 Malic enzyme IPR012302 305 557 PF03949 Malic enzyme HMMSmart IPR012302 305 557 SM00919 Malic enzyme
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001014811 604 604626 99.5 100.0 NP_006671 604 604626 99.5 100.0 XP_005273774 604 604626 99.5 100.0 NP_001155058 604 604626 99.5 100.0 XP_016872625 724 604626 99.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE2477 Download

>KIEE2477  1812 bp
ATGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGC
GCCCTCCCGCGCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCG
GCGCGCCCTGTGCCCCTGAAGAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAAC
AAGGGGATGGCCTTTACCCTTGAAGAAAGGCTGCAGCTTGGAATCCACGGCCTAATCCCG
CCCTGCTTTCTGGGCCAGGACGTCCAGCTCCTCCGAATCATGGGATATTACGAGCGGCAG
CAGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAACGAGAAGCTC
TTCTACCGAGTGCTGACTTCGGATGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACC
GTGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATC
ACCATTCATGACAAAGGTCATCTTGCAACAATGCTGAATTCTTGGCCAGAAGACAATATT
AAGGCCGTGGTGGTGACTGATGGGGAGCGCATCCTGGGCCTGGGAGACCTGGGCTGCTAC
GGCATGGGCATCCCTGTGGGCAAGCTGGCCCTGTACACGGCATGCGGAGGGGTGAACCCG
CAGCAGTGCCTCCCTGTGCTGCTGGACGTCGGCACCAACAATGAGGAGCTGCTCAGAGAC
CCTCTGTACATCGGCCTGAAACACCAGCGCGTGCACGGGAAGGCATACGATGACTTGCTG
GATGAGTTCATGCAGGCTGTGACAGACAAGTTTGGAATAAATTGCCTCATCCAATTTGAA
GACTTCGCCAATGCCAATGCCTTCCGCCTGCTCAACAAATACCGTAACAAGTACTGCATG
TTCAATGATGACATCCAAGGCACAGCCTCCGTTGCTGTGGCAGGGATCTTGGCTGCTCTG
CGAATCACCAACAACAAGCTTTCCAATCACGTGTTTGTTTTCCAAGGTGCAGGCGAGGCA
GCTATGGGCATTGCCCACCTCCTTGTCATGGCCCTAGAGAAAGAAGGTGTACCGAAGGCA
GAGGCCACAAGAAAGATCTGGATGGTGGACTCTAAAGGGCTCATTGTCAAGGGGAGGAGC
CACCTGAACCATGAAAAGGAGATGTTTGCCCAAGACCATCCTGAAGTCAACTCCCTGGAG
GAGGTGGTGAGGCTGGTGAAGCCCACAGCCATCATAGGTGTTGCTGCCATCGCAGGAGCC
TTCACGGAGCAGATTCTGAGGGACATGGCCTCCTTCCACGAGCGCCCTATCATCTTTGCC
CTGAGCAACCCCACCAGCAAGGCCGAGTGCACGGCTGAGAAGTGCTACCGGGTCACCGAG
GGCCGAGGGATTTTTGCCAGTGGAAGTCCTTTTAAGAGTGTGACTCTGGAAGATGGCAAG
ACCTTCATTCCTGGGCAGGGAAACAATGCTTACGTGTTCCCCGGGGTGGCACTGGGAGTC
ATCGCCGGCGGGATCCGGCACATCCCAGATGAGATCTTCCTCCTGACAGCAGAGCAAATT
GCCCAGGAAGTCTCTGAGCAGCATCTGTCCCAGGGGAGACTCTATCCACCACTCAGCACC
ATCCGAGACGTGTCTTTGAGAATTGCCATCAAAGTTCTCGACTACGCGTACAAACACAAC
CTGGCTTCCTACTACCCAGAGCCTAAGGACAAGGAGGCTTTTGTAAGATCCCTGGTCTAC
ACTCCAGACTATGACTCCTTTACACTGGACAGCTACACTTGGCCCAAGGAAGCCATGAAT
GTTCAGACGGTC

ORF Protein

Cloned ORF protein sequence for pF1KE2477 Download

>KIEE2477  604 aa
MGAALGTGTRLAPWPGRACGALPRWTPTAPAQGCHSKPGPARPVPLKKRGYDVTRNPHLN
KGMAFTLEERLQLGIHGLIPPCFLGQDVQLLRIMGYYERQQSDLDKYIILMTLQDRNEKL
FYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNI
KAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRD
PLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCM
FNDDIQGTASVAVAGILAALRITNNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKA
EATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGA
FTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGK
TFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLST
IRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMN
VQTV

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE2477 Download

>pF1KE2477  4925 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGGTGCCGCG
CTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGCGCCCTCCCGCGC
TGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCGGCGCGCCCTGTG
CCCCTGAAGAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAACAAGGGGATGGCC
TTTACCCTTGAAGAAAGGCTGCAGCTTGGAATCCACGGCCTAATCCCGCCCTGCTTTCTG
GGCCAGGACGTCCAGCTCCTCCGAATCATGGGATATTACGAGCGGCAGCAGAGTGACCTG
GACAAGTACATCATTCTCATGACACTCCAAGACCGGAACGAGAAGCTCTTCTACCGAGTG
CTGACTTCGGATGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACCGTGGGGCTGGCC
TGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATCACCATTCATGAC
AAAGGTCATCTTGCAACAATGCTGAATTCTTGGCCAGAAGACAATATTAAGGCCGTGGTG
GTGACTGATGGGGAGCGCATCCTGGGCCTGGGAGACCTGGGCTGCTACGGCATGGGCATC
CCTGTGGGCAAGCTGGCCCTGTACACGGCATGCGGAGGGGTGAACCCGCAGCAGTGCCTC
CCTGTGCTGCTGGACGTCGGCACCAACAATGAGGAGCTGCTCAGAGACCCTCTGTACATC
GGCCTGAAACACCAGCGCGTGCACGGGAAGGCATACGATGACTTGCTGGATGAGTTCATG
CAGGCTGTGACAGACAAGTTTGGAATAAATTGCCTCATCCAATTTGAAGACTTCGCCAAT
GCCAATGCCTTCCGCCTGCTCAACAAATACCGTAACAAGTACTGCATGTTCAATGATGAC
ATCCAAGGCACAGCCTCCGTTGCTGTGGCAGGGATCTTGGCTGCTCTGCGAATCACCAAC
AACAAGCTTTCCAATCACGTGTTTGTTTTCCAAGGTGCAGGCGAGGCAGCTATGGGCATT
GCCCACCTCCTTGTCATGGCCCTAGAGAAAGAAGGTGTACCGAAGGCAGAGGCCACAAGA
AAGATCTGGATGGTGGACTCTAAAGGGCTCATTGTCAAGGGGAGGAGCCACCTGAACCAT
GAAAAGGAGATGTTTGCCCAAGACCATCCTGAAGTCAACTCCCTGGAGGAGGTGGTGAGG
CTGGTGAAGCCCACAGCCATCATAGGTGTTGCTGCCATCGCAGGAGCCTTCACGGAGCAG
ATTCTGAGGGACATGGCCTCCTTCCACGAGCGCCCTATCATCTTTGCCCTGAGCAACCCC
ACCAGCAAGGCCGAGTGCACGGCTGAGAAGTGCTACCGGGTCACCGAGGGCCGAGGGATT
TTTGCCAGTGGAAGTCCTTTTAAGAGTGTGACTCTGGAAGATGGCAAGACCTTCATTCCT
GGGCAGGGAAACAATGCTTACGTGTTCCCCGGGGTGGCACTGGGAGTCATCGCCGGCGGG
ATCCGGCACATCCCAGATGAGATCTTCCTCCTGACAGCAGAGCAAATTGCCCAGGAAGTC
TCTGAGCAGCATCTGTCCCAGGGGAGACTCTATCCACCACTCAGCACCATCCGAGACGTG
TCTTTGAGAATTGCCATCAAAGTTCTCGACTACGCGTACAAACACAACCTGGCTTCCTAC
TACCCAGAGCCTAAGGACAAGGAGGCTTTTGTAAGATCCCTGGTCTACACTCCAGACTAT
GACTCCTTTACACTGGACAGCTACACTTGGCCCAAGGAAGCCATGAATGTTCAGACGGTC
GTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA
AGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGA
GCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA
AGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCA
TGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGAT
AGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGT
GTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAA
GAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATG
GCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGA
TGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGG
GTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCC
GTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGT
GCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTT
CCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGC
GAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATC
ATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCAC
CAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAG
GATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAG
GCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAAT
ATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCG
GACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAA
TGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCC
TTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACC
AAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC
AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA
AAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAA
TCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCC
CCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTC
CGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAG
TTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGA
CCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATC
GCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTAC
AGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTG
CGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACA
AACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAA
AGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAA
CTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTT
ATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATG
AAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCAT
AGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGT
TGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAA
TTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCA
GTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGAT
TAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGT
GGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT
GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGT
CGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGA
CAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAG
GACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCC
TTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCAT