ARHGAP10 ORF clone pF1KE4224

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Flexi ORF Clone pF1KE4224

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Clone info.

Species

human

Clone Name

pF1KE4224 Sequence (with vector) Restriction Map

Product ID

FXC31709

HaloTag Clone PID

FHC31709

ORF Sequence

DNA sequence (2358 bp)
Protein sequence (786 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4224

Symbol

ARHGAP10

Alias : GRAF2, PS-GAP, PSGAP

Description

Rho GTPase activating protein 10

Original Clone ID

cp94224

Note

We obtained this clone using the E.coli strain JM109 at 25 deg C. This plasmid seems unstable at 37 deg C.

Features of the cloned ORF sequence

Description

Length: 2358 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 786 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34075.1 1.3e-180 100.0 ARHGAP10 CCDS4277.1 6.2e-86 55.4 ARHGAP26 CCDS47297.1 8.2e-86 58.2 ARHGAP26 CCDS14388.1 8.2e-42 44.5 OPHN1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000198 398 548 PF00620 Rho GTPase-activating protein domain IPR001452 734 779 PF00018 Src homology-3 domain HMMSmart IPR001849 266 374 SM00233 Pleckstrin homology domain IPR000198 393 571 SM00324 Rho GTPase-activating protein domain IPR001452 731 786 SM00326 Src homology-3 domain ProfileScan IPR001849 265 372 PS50003 Pleckstrin homology domain IPR000198 389 574 PS50238 Rho GTPase-activating protein domain IPR001452 728 786 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_078881 786 609746 100.0 100.0 XP_005263272 735 609746 93.5 100.0 XP_016864091 694 609746 100.0 83.7 NP_055886 814 605370,607785 55.4 100.0 NP_001129080 759 605370,607785 58.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE4224 Download

>KIEE4224  2358 bp
ATGGGGCTGCAGCCCCTGGAGTTCAGCGACTGCTACCTCGACAGCCCGTGGTTCCGGGAG
AGGATCCGCGCTCACGAAGCGGAACTCGAGAGGACCAACAAGTTCATCAAAGAGCTCATT
AAGGACGGGAAGAACCTCATCGCTGCGACGAAAAGTCTGTCAGTGGCCCAGCGGAAGTTT
GCTCATTCACTCAGAGACTTTAAGTTTGAGTTTATCGGTGATGCTGTGACAGATGATGAA
CGATGCATAGATGCTTCCTTACGTGAATTTTCAAATTTTTTGAAGAATCTGGAGGAACAG
AGAGAAATTATGGCATTAAGTGTAACTGAAACCCTGATTAAACCCTTGGAAAAATTCAGA
AAAGAGCAACTTGGAGCTGTAAAGGAAGAAAAAAAGAAGTTTGACAAAGAGACAGAAAAG
AATTATAGTCTAATTGATAAACATTTGAATTTATCAGCAAAAAAGAAAGACTCACATTTA
CAAGAGGCAGATATCCAAGTAGAGCAGAACCGGCAACACTTCTATGAACTGTCTCTCGAG
TATGTGTGTAAGCTGCAGGAAATCCAAGAAAGAAAGAAGTTTGAGTTTGTGGAACCTATG
CTGTCATTTTTTCAGGGGATGTTTACCTTCTATCATCAGGGCCATGAACTTGCCAAAGAC
TTCAATCACTACAAAATGGAACTACAGATCAACATTCAGAATACACGGAATCGATTTGAA
GGAACAAGGTCAGAAGTGGAAGAGCTCATGAACAAAATCAGACAGAATCCCAAGGACCAC
AAACGAGCAAGTCAGTTTACAGCCGAAGGCTACCTGTATGTCCAGGAAAAAAGGCCTGCT
CCGTTTGGTTCCAGTTGGGTCAAACACTATTGCATGTATCGAAAAGCAGCAAAGAAGTTC
AACATGATCCCATTTGAGCACAGATCTGGAGGGAAACTTGGGGACGGAGAGGTGTTCTTT
TTGAAAGAATGTACCAAGAGGCATACTGACTCCATTGACAGAAGGTTTTGTTTTGACATA
GAAGCTGCTGATCGGCCTGGCGTTTCCTTGACCATGCAGGCATTTTCCGAAGAGGAAAGG
AAGCAGTGGTTGGAAGCTCTGGGTGGAAAGGAAGCTCTGTCCCATAGTTTTAATACAGCC
ATCATCCCAAGACCAGAAGGAAATGCACAGTTGGATAAGATGGGGTTCACAATTATCAGA
AAATGCATCAGTGCCGTTGAAACACGAGGTATAAATGACCAAGGATTGTACAGAGTTGTG
GGGGTGAGTTCAAAGGTCCAGAGACTTCTGAGTATGTTGATGGATGTAAAAACATGCAAT
GAGGTGGACCTGGAGAATTCTGCAGATTGGGAAGTGAAGACAATAACAAGTGCCTTGAAA
CAGTATTTGAGGAGTCTTCCAGAGCCTCTCATGACCTATGAGTTACATGGAGATTTCATT
GTTCCAGCCAAAAGCGGCAGCCCAGAATCTCGTGTTAATGCGATCCATTTCTTGGTACAC
AAACTGCCAGAGAAGAATAAAGAGATGTTGGATATTTTGGTGAAACACTTAACAAATGTT
TCAAATCACTCCAAGCAGAACCTGATGACTGTGGCAAACTTAGGAGTGGTGTTTGGACCA
ACTCTGATGAGGCCACAGGAAGAAACTGTCGCTGCCCTCATGGACTTGAAGTTTCAGAAT
ATTGTTGTGGAAATCTTAATTGAAAACCATGAAAAGATTTTTCGGACGCCGCCCGATACT
ACATTCCCTGAGCCCACCTGCCTGTCAGCATCACCCCCAAATGCGCCACCAAGGCAGTCG
AAGAGACAAGGCCAGAGAACCAAGAGGCCCGTGGCCGTCTACAATCTTTGTCTGGAGCTG
GAAGATGGTGACAATCCTTACCCTTCCAAGGAGGACACCCCTACCAGCAGTCTGGACTCA
CTTTCCTCCCCGTCTCCCGTGACTACAGCTGTCCCTGGGCCTCCTGGACCAGACAAAAAC
CACCTTCTGGCAGATGGAGGGAGCTTTGGAGACTGGGCATCCACTATCCCAGGCCAGACC
CGATCGTCTATGGTCCAGTGGCTTAACCCACAGTCTCCAACCACAACAAGCTCCAACTCA
GCTGTGACACCTCTTTCACCCGGGTCGTCCCCTTTCCCCTTTTCTCCTCCTGCTACTGTA
GCGGACAAGCCACCTGAAAGCATCCGCAGTCGGAAGGCTCGAGCCGTGTATCCGTGTGAA
GCAGAACACAGCTCGGAATTATCTTTTGAAATAGGAGCAATTTTTGAGGATGTACAAACC
TCCAGGGAACCTGGCTGGCTAGAAGGGACTCTGAACGGCAAGAGGGGGCTGATTCCACAG
AACTACGTCAAGCTGCTG

ORF Protein

Cloned ORF protein sequence for pF1KE4224 Download

>KIEE4224  786 aa
MGLQPLEFSDCYLDSPWFRERIRAHEAELERTNKFIKELIKDGKNLIAATKSLSVAQRKF
AHSLRDFKFEFIGDAVTDDERCIDASLREFSNFLKNLEEQREIMALSVTETLIKPLEKFR
KEQLGAVKEEKKKFDKETEKNYSLIDKHLNLSAKKKDSHLQEADIQVEQNRQHFYELSLE
YVCKLQEIQERKKFEFVEPMLSFFQGMFTFYHQGHELAKDFNHYKMELQINIQNTRNRFE
GTRSEVEELMNKIRQNPKDHKRASQFTAEGYLYVQEKRPAPFGSSWVKHYCMYRKAAKKF
NMIPFEHRSGGKLGDGEVFFLKECTKRHTDSIDRRFCFDIEAADRPGVSLTMQAFSEEER
KQWLEALGGKEALSHSFNTAIIPRPEGNAQLDKMGFTIIRKCISAVETRGINDQGLYRVV
GVSSKVQRLLSMLMDVKTCNEVDLENSADWEVKTITSALKQYLRSLPEPLMTYELHGDFI
VPAKSGSPESRVNAIHFLVHKLPEKNKEMLDILVKHLTNVSNHSKQNLMTVANLGVVFGP
TLMRPQEETVAALMDLKFQNIVVEILIENHEKIFRTPPDTTFPEPTCLSASPPNAPPRQS
KRQGQRTKRPVAVYNLCLELEDGDNPYPSKEDTPTSSLDSLSSPSPVTTAVPGPPGPDKN
HLLADGGSFGDWASTIPGQTRSSMVQWLNPQSPTTTSSNSAVTPLSPGSSPFPFSPPATV
ADKPPESIRSRKARAVYPCEAEHSSELSFEIGAIFEDVQTSREPGWLEGTLNGKRGLIPQ
NYVKLL

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE4224 Download

>pF1KE4224  5471 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGGGCTGCAG
CCCCTGGAGTTCAGCGACTGCTACCTCGACAGCCCGTGGTTCCGGGAGAGGATCCGCGCT
CACGAAGCGGAACTCGAGAGGACCAACAAGTTCATCAAAGAGCTCATTAAGGACGGGAAG
AACCTCATCGCTGCGACGAAAAGTCTGTCAGTGGCCCAGCGGAAGTTTGCTCATTCACTC
AGAGACTTTAAGTTTGAGTTTATCGGTGATGCTGTGACAGATGATGAACGATGCATAGAT
GCTTCCTTACGTGAATTTTCAAATTTTTTGAAGAATCTGGAGGAACAGAGAGAAATTATG
GCATTAAGTGTAACTGAAACCCTGATTAAACCCTTGGAAAAATTCAGAAAAGAGCAACTT
GGAGCTGTAAAGGAAGAAAAAAAGAAGTTTGACAAAGAGACAGAAAAGAATTATAGTCTA
ATTGATAAACATTTGAATTTATCAGCAAAAAAGAAAGACTCACATTTACAAGAGGCAGAT
ATCCAAGTAGAGCAGAACCGGCAACACTTCTATGAACTGTCTCTCGAGTATGTGTGTAAG
CTGCAGGAAATCCAAGAAAGAAAGAAGTTTGAGTTTGTGGAACCTATGCTGTCATTTTTT
CAGGGGATGTTTACCTTCTATCATCAGGGCCATGAACTTGCCAAAGACTTCAATCACTAC
AAAATGGAACTACAGATCAACATTCAGAATACACGGAATCGATTTGAAGGAACAAGGTCA
GAAGTGGAAGAGCTCATGAACAAAATCAGACAGAATCCCAAGGACCACAAACGAGCAAGT
CAGTTTACAGCCGAAGGCTACCTGTATGTCCAGGAAAAAAGGCCTGCTCCGTTTGGTTCC
AGTTGGGTCAAACACTATTGCATGTATCGAAAAGCAGCAAAGAAGTTCAACATGATCCCA
TTTGAGCACAGATCTGGAGGGAAACTTGGGGACGGAGAGGTGTTCTTTTTGAAAGAATGT
ACCAAGAGGCATACTGACTCCATTGACAGAAGGTTTTGTTTTGACATAGAAGCTGCTGAT
CGGCCTGGCGTTTCCTTGACCATGCAGGCATTTTCCGAAGAGGAAAGGAAGCAGTGGTTG
GAAGCTCTGGGTGGAAAGGAAGCTCTGTCCCATAGTTTTAATACAGCCATCATCCCAAGA
CCAGAAGGAAATGCACAGTTGGATAAGATGGGGTTCACAATTATCAGAAAATGCATCAGT
GCCGTTGAAACACGAGGTATAAATGACCAAGGATTGTACAGAGTTGTGGGGGTGAGTTCA
AAGGTCCAGAGACTTCTGAGTATGTTGATGGATGTAAAAACATGCAATGAGGTGGACCTG
GAGAATTCTGCAGATTGGGAAGTGAAGACAATAACAAGTGCCTTGAAACAGTATTTGAGG
AGTCTTCCAGAGCCTCTCATGACCTATGAGTTACATGGAGATTTCATTGTTCCAGCCAAA
AGCGGCAGCCCAGAATCTCGTGTTAATGCGATCCATTTCTTGGTACACAAACTGCCAGAG
AAGAATAAAGAGATGTTGGATATTTTGGTGAAACACTTAACAAATGTTTCAAATCACTCC
AAGCAGAACCTGATGACTGTGGCAAACTTAGGAGTGGTGTTTGGACCAACTCTGATGAGG
CCACAGGAAGAAACTGTCGCTGCCCTCATGGACTTGAAGTTTCAGAATATTGTTGTGGAA
ATCTTAATTGAAAACCATGAAAAGATTTTTCGGACGCCGCCCGATACTACATTCCCTGAG
CCCACCTGCCTGTCAGCATCACCCCCAAATGCGCCACCAAGGCAGTCGAAGAGACAAGGC
CAGAGAACCAAGAGGCCCGTGGCCGTCTACAATCTTTGTCTGGAGCTGGAAGATGGTGAC
AATCCTTACCCTTCCAAGGAGGACACCCCTACCAGCAGTCTGGACTCACTTTCCTCCCCG
TCTCCCGTGACTACAGCTGTCCCTGGGCCTCCTGGACCAGACAAAAACCACCTTCTGGCA
GATGGAGGGAGCTTTGGAGACTGGGCATCCACTATCCCAGGCCAGACCCGATCGTCTATG
GTCCAGTGGCTTAACCCACAGTCTCCAACCACAACAAGCTCCAACTCAGCTGTGACACCT
CTTTCACCCGGGTCGTCCCCTTTCCCCTTTTCTCCTCCTGCTACTGTAGCGGACAAGCCA
CCTGAAAGCATCCGCAGTCGGAAGGCTCGAGCCGTGTATCCGTGTGAAGCAGAACACAGC
TCGGAATTATCTTTTGAAATAGGAGCAATTTTTGAGGATGTACAAACCTCCAGGGAACCT
GGCTGGCTAGAAGGGACTCTGAACGGCAAGAGGGGGCTGATTCCACAGAACTACGTCAAG
CTGCTGGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGG
CATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCAC
CGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTT
GCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTA
TCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGT
CCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTT
CTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCT
TCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAAC
TGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAG
ACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCC
GCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGAT
GCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTG
TCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACG
GGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTA
TTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTA
TCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTC
GACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTC
GATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGG
CTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTG
CCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGT
GTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGC
GGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGC
ATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGA
CCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCAC
AGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA
CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA
CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGC
GTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA
CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA
TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCA
GCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGA
CTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGG
TGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG
TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG
CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG
AAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA
CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGAT
CCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAA
ACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGG
TTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTG
GTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGC
GTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAA
ACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGAT
ACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAG
CGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGG
TAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGG
CTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA
GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGC
GGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGG
ATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCAT