RACGAP1 ORF clone pFN21AA1478

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Halotag ORF Clone pFN21AA1478

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Clone info.

Species

human

Clone Name

pFN21AA1478 Sequence (with vector) Restriction Map

Product ID

FHC00237

Flexi Clone PID

FXC00237

Original Clone PID

ORK00237

ORF Sequence

DNA sequence (1896 bp)
Protein sequence (632 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384608]

Gene

KIAA1478

Symbol

RACGAP1

Alias : CYK4, HsCYK-4, ID-GAP, MgcRacGAP

Description

Rac GTPase activating protein 1, transcript variant 1

Original Clone ID

ef04341

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1896 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 632 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8795.1 4.9e-204 100.0 RACGAP1 CCDS56147.1 8.6e-17 33.6 CHN1 CCDS46455.1 1.1e-16 33.6 CHN1 CCDS46454.1 2.4e-16 33.2 CHN1 CCDS47566.1 1.2e-15 31.2 CHN2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002219 287 336 PF00130 Protein kinase C-like IPR000198 363 507 PF00620 Rho GTPase-activating protein domain HMMSmart IPR002219 287 335 SM00109 Protein kinase C-like IPR000198 360 536 SM00324 Rho GTPase-activating protein domain ProfileScan IPR002219 286 335 PS50081 Protein kinase C-like IPR000198 349 539 PS50238 Rho GTPase-activating protein domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001119576 632 604980 100.0 100.0 NP_001306932 632 604980 100.0 100.0 XP_011536540 632 604980 100.0 100.0 XP_006719422 632 604980 100.0 100.0 NP_001306930 632 604980 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1478 Download

>KIAA1478  1896 bp
ATGGATACTATGATGCTGAATGTGCGGAATCTGTTTGAGCAGCTTGTGCGCCGGGTGGAG
ATTCTCAGTGAAGGAAATGAAGTCCAATTTATCCAGTTGGCGAAGGACTTTGAGGATTTC
CGTAAAAAGTGGCAGAGGACTGACCATGAGCTGGGGAAATACAAGGATCTTTTGATGAAA
GCAGAGACTGAGCGAAGTGCTCTGGATGTTAAGCTGAAGCATGCACGTAATCAGGTGGAT
GTAGAGATCAAACGGAGACAGAGAGCTGAGGCTGACTGCGAAAAGCTGGAACGACAGATT
CAGCTGATTCGAGAGATGCTCATGTGTGACACATCTGGCAGCATTCAACTAAGCGAGGAG
CAAAAATCAGCTCTGGCTTTTCTCAACAGAGGCCAACCATCCAGCAGCAATGCTGGGAAC
AAAAGACTATCAACCATTGATGAATCTGGTTCCATTTTATCAGATATCAGCTTTGACAAG
ACTGATGAATCACTGGATTGGGACTCTTCTTTGGTGAAGACTTTCAAACTGAAGAAGAGA
GAAAAGAGGCGCTCTACTAGCCGACAGTTTGTTGATGGTCCCCCTGGACCTGTAAAGAAA
ACTCGTTCCATTGGCTCTGCAGTAGACCAGGGGAATGAATCCATAGTTGCAAAAACTACA
GTGACTGTTCCCAATGATGGCGGGCCCATCGAAGCTGTGTCCACTATTGAGACTGTGCCA
TATTGGACCAGGAGCCGAAGGAAAACAGGTACTTTACAACCTTGGAACAGTGACTCCACC
CTGAACAGCAGGCAGCTGGAGCCAAGAACTGAGACAGACAGTGTGGGCACGCCACAGAGT
AATGGAGGGATGCGCCTGCATGACTTTGTTTCTAAGACGGTTATTAAACCTGAATCCTGT
GTTCCATGTGGAAAGCGGATAAAATTTGGCAAATTATCTCTGAAGTGTCGAGACTGTCGT
GTGGTCTCTCATCCAGAATGTCGGGACCGCTGTCCCCTTCCCTGCATTCCTACCCTGATA
GGAACACCTGTCAAGATTGGAGAGGGAATGCTGGCAGACTTTGTGTCCCAGACTTCTCCA
ATGATCCCCTCCATTGTTGTGCATTGTGTAAATGAGATTGAGCAAAGAGGTCTGACTGAG
ACAGGCCTGTATAGGATCTCTGGCTGTGACCGCACAGTAAAAGAGCTGAAAGAGAAATTC
CTCAGAGTGAAAACTGTACCCCTCCTCAGCAAAGTGGATGATATCCATGCTATCTGTAGC
CTTCTAAAAGACTTTCTTCGAAACCTCAAAGAACCTCTTCTGACCTTTCGCCTTAACAGA
GCCTTTATGGAAGCAGCAGAAATCACAGATGAAGACAACAGCATAGCTGCCATGTACCAA
GCTGTTGGTGAACTGCCCCAGGCCAACAGGGACACATTAGCTTTCCTCATGATTCACTTG
CAGAGAGTGGCTCAGAGTCCACATACTAAAATGGATGTTGCCAATCTGGCTAAAGTCTTT
GGCCCTACAATAGTGGCCCATGCTGTGCCCAATCCAGACCCAGTGACAATGTTACAGGAC
ATCAAGCGTCAACCCAAGGTGGTTGAGCGCCTGCTTTCCTTGCCTCTGGAGTATTGGAGT
CAGTTCATGATGGTGGAGCAAGAGAACATTGACCCCCTACATGTCATTGAAAACTCAAAT
GCCTTTTCAACACCACAGACACCAGATATTAAAGTGAGTTTACTGGGACCTGTGACCACT
CCTGAACATCAGCTTCTCAAGACTCCTTCATCTAGTTCCCTGTCACAGAGAGTCCGTTCC
ACCCTCACCAAGAACACTCCTAGATTTGGGAGCAAAAGCAAGTCTGCCACTAACCTAGGA
CGACAAGGCAACTTTTTTGCTTCTCCAATGCTCAAG

ORF Protein

Cloned ORF protein sequence for pFN21AA1478 Download

>KIAA1478  632 aa
MDTMMLNVRNLFEQLVRRVEILSEGNEVQFIQLAKDFEDFRKKWQRTDHELGKYKDLLMK
AETERSALDVKLKHARNQVDVEIKRRQRAEADCEKLERQIQLIREMLMCDTSGSIQLSEE
QKSALAFLNRGQPSSSNAGNKRLSTIDESGSILSDISFDKTDESLDWDSSLVKTFKLKKR
EKRRSTSRQFVDGPPGPVKKTRSIGSAVDQGNESIVAKTTVTVPNDGGPIEAVSTIETVP
YWTRSRRKTGTLQPWNSDSTLNSRQLEPRTETDSVGTPQSNGGMRLHDFVSKTVIKPESC
VPCGKRIKFGKLSLKCRDCRVVSHPECRDRCPLPCIPTLIGTPVKIGEGMLADFVSQTSP
MIPSIVVHCVNEIEQRGLTETGLYRISGCDRTVKELKEKFLRVKTVPLLSKVDDIHAICS
LLKDFLRNLKEPLLTFRLNRAFMEAAEITDEDNSIAAMYQAVGELPQANRDTLAFLMIHL
QRVAQSPHTKMDVANLAKVFGPTIVAHAVPNPDPVTMLQDIKRQPKVVERLLSLPLEYWS
QFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRS
TLTKNTPRFGSKSKSATNLGRQGNFFASPMLK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1478 Download

>pFN21AA1478  6601 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATACTATGATGCTGAATGTGCGGAATCTGTT
TGAGCAGCTTGTGCGCCGGGTGGAGATTCTCAGTGAAGGAAATGAAGTCCAATTTATCCA
GTTGGCGAAGGACTTTGAGGATTTCCGTAAAAAGTGGCAGAGGACTGACCATGAGCTGGG
GAAATACAAGGATCTTTTGATGAAAGCAGAGACTGAGCGAAGTGCTCTGGATGTTAAGCT
GAAGCATGCACGTAATCAGGTGGATGTAGAGATCAAACGGAGACAGAGAGCTGAGGCTGA
CTGCGAAAAGCTGGAACGACAGATTCAGCTGATTCGAGAGATGCTCATGTGTGACACATC
TGGCAGCATTCAACTAAGCGAGGAGCAAAAATCAGCTCTGGCTTTTCTCAACAGAGGCCA
ACCATCCAGCAGCAATGCTGGGAACAAAAGACTATCAACCATTGATGAATCTGGTTCCAT
TTTATCAGATATCAGCTTTGACAAGACTGATGAATCACTGGATTGGGACTCTTCTTTGGT
GAAGACTTTCAAACTGAAGAAGAGAGAAAAGAGGCGCTCTACTAGCCGACAGTTTGTTGA
TGGTCCCCCTGGACCTGTAAAGAAAACTCGTTCCATTGGCTCTGCAGTAGACCAGGGGAA
TGAATCCATAGTTGCAAAAACTACAGTGACTGTTCCCAATGATGGCGGGCCCATCGAAGC
TGTGTCCACTATTGAGACTGTGCCATATTGGACCAGGAGCCGAAGGAAAACAGGTACTTT
ACAACCTTGGAACAGTGACTCCACCCTGAACAGCAGGCAGCTGGAGCCAAGAACTGAGAC
AGACAGTGTGGGCACGCCACAGAGTAATGGAGGGATGCGCCTGCATGACTTTGTTTCTAA
GACGGTTATTAAACCTGAATCCTGTGTTCCATGTGGAAAGCGGATAAAATTTGGCAAATT
ATCTCTGAAGTGTCGAGACTGTCGTGTGGTCTCTCATCCAGAATGTCGGGACCGCTGTCC
CCTTCCCTGCATTCCTACCCTGATAGGAACACCTGTCAAGATTGGAGAGGGAATGCTGGC
AGACTTTGTGTCCCAGACTTCTCCAATGATCCCCTCCATTGTTGTGCATTGTGTAAATGA
GATTGAGCAAAGAGGTCTGACTGAGACAGGCCTGTATAGGATCTCTGGCTGTGACCGCAC
AGTAAAAGAGCTGAAAGAGAAATTCCTCAGAGTGAAAACTGTACCCCTCCTCAGCAAAGT
GGATGATATCCATGCTATCTGTAGCCTTCTAAAAGACTTTCTTCGAAACCTCAAAGAACC
TCTTCTGACCTTTCGCCTTAACAGAGCCTTTATGGAAGCAGCAGAAATCACAGATGAAGA
CAACAGCATAGCTGCCATGTACCAAGCTGTTGGTGAACTGCCCCAGGCCAACAGGGACAC
ATTAGCTTTCCTCATGATTCACTTGCAGAGAGTGGCTCAGAGTCCACATACTAAAATGGA
TGTTGCCAATCTGGCTAAAGTCTTTGGCCCTACAATAGTGGCCCATGCTGTGCCCAATCC
AGACCCAGTGACAATGTTACAGGACATCAAGCGTCAACCCAAGGTGGTTGAGCGCCTGCT
TTCCTTGCCTCTGGAGTATTGGAGTCAGTTCATGATGGTGGAGCAAGAGAACATTGACCC
CCTACATGTCATTGAAAACTCAAATGCCTTTTCAACACCACAGACACCAGATATTAAAGT
GAGTTTACTGGGACCTGTGACCACTCCTGAACATCAGCTTCTCAAGACTCCTTCATCTAG
TTCCCTGTCACAGAGAGTCCGTTCCACCCTCACCAAGAACACTCCTAGATTTGGGAGCAA
AAGCAAGTCTGCCACTAACCTAGGACGACAAGGCAACTTTTTTGCTTCTCCAATGCTCAA
GGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
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