PRPF4 ORF clone pFN21AB5305

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5305

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Clone info.

Species

human

Clone Name

pFN21AB5305 Sequence (with vector) Restriction Map

Product ID

FHC10807

ORF Sequence

DNA sequence (1563 bp)
Protein sequence (521 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590453]

Gene

KIBB5305

Symbol

PRPF4

Alias : HPRP4, HPRP4P, PRP4, Prp4p, RP70, SNRNP60

Description

pre-mRNA processing factor 4, transcript variant 2

Original Clone ID

bm03275

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1563 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 521 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS59142.1 1e-156 100.0 PRPF4 CCDS6791.1 3.5e-156 99.8 PRPF4 CCDS2470.1 2e-18 30.3 DAW1 CCDS6981.1 6.9e-15 26.1 WDR5 CCDS3012.1 3e-14 27.1 WDR5B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR020472 295 309 PR00320 G-protein beta WD-40 repeat IPR020472 421 435 PR00320 G-protein beta WD-40 repeat IPR020472 464 478 PR00320 G-protein beta WD-40 repeat HMMPfam IPR014906 107 136 PF08799 Pre-mRNA processing factor 4 (PRP4)-like IPR019781 228 258 PF00400 WD40 repeat IPR019781 263 308 PF00400 WD40 repeat IPR019781 313 350 PF00400 WD40 repeat IPR019781 361 392 PF00400 WD40 repeat IPR019781 396 434 PF00400 WD40 repeat IPR019781 439 476 PF00400 WD40 repeat IPR019781 481 518 PF00400 WD40 repeat HMMSmart IPR003648 102 154 SM00500 Splicing factor motif IPR001680 219 258 SM00320 WD40 repeat IPR001680 261 308 SM00320 WD40 repeat IPR001680 311 350 SM00320 WD40 repeat IPR001680 353 392 SM00320 WD40 repeat IPR001680 395 434 SM00320 WD40 repeat IPR001680 437 477 SM00320 WD40 repeat IPR001680 480 519 SM00320 WD40 repeat ProfileScan IPR017986 226 521 PS50294 WD40-repeat-containing domain IPR019782 268 317 PS50082 WD40 repeat 2 IPR019782 318 359 PS50082 WD40 repeat 2 IPR019782 360 401 PS50082 WD40 repeat 2 IPR019782 402 443 PS50082 WD40 repeat 2 IPR019782 444 476 PS50082 WD40 repeat 2 IPR019782 487 521 PS50082 WD40 repeat 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001231855 521 607795,615922 100.0 100.0 NP_004688 522 607795,615922 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5305 Download

>KIBB5305  1563 bp
ATGGCTTCCTCGCGAGCCTCTTCCACGGCAACCAAAACTAAAGCACCCGACGACTTAGTT
GCTCCGGTCGTGAAGAAACCACACATCTATTATGGAAGTTTGGAAGAGAAGGAGAGGGAG
CGTCTGGCCAAAGGAGAGTCTGGGATTTTGGGGAAAGACGGACTTAAAGCAGGGATCGAA
GCTGGAAATATTAATATAACCTCTGGAGAAGTGTTTGAAATTGAAGAGCATATCAGCGAG
CGACAGGCAGAAGTATTGGCTGAGTTTGAGAGAAGGAAGCGAGCCCGGCAGATCAATGTT
TCCACAGATGACTCAGAGGTCAAAGCTTGCCTTAGAGCCTTGGGGGAACCCATCACACTT
TTTGGAGAGGGTCCTGCTGAAAGAAGAGAAAGGTTAAGAAATATCCTCTCAGTTGTCGGT
ACTGATGCCTTGAAAAAGACCAAAAAGGATGATGAGAAGTCTAAAAAGTCCAAAGAAGAG
TATCAGCAAACCTGGTATCATGAAGGACCAAATAGCTTGAAGGTGGCAAGACTATGGATT
GCTAATTATTCGTTGCCCAGGGCAATGAAACGCTTGGAAGAGGCCCGACTCCATAAGGAG
ATTCCTGAGACAACAAGGACCTCCCAGATGCAAGAGCTGCACAAGTCTCTCCGGTCTTTG
AATAATTTTTGCAGTCAGATTGGGGATGATCGGCCTATCTCCTACTGTCACTTTAGTCCC
AATTCCAAGATGCTGGCCACAGCTTGTTGGAGTGGGCTTTGCAAGCTCTGGTCTGTTCCT
GATTGCAACCTCCTTCACACTCTTCGAGGGCATAACACAAATGTAGGAGCAATTGTATTC
CATCCCAAATCCACTGTCTCCTTGGACCCAAAAGATGTCAACCTGGCCTCTTGTGCGGCT
GATGGCTCTGTGAAGCTTTGGAGTCTCGACAGTGATGAACCAGTGGCAGATATTGAAGGC
CATACAGTGCGTGTGGCGCGGGTAATGTGGCATCCTTCAGGACGTTTCCTGGGCACCACC
TGCTATGACCGTTCATGGCGCTTATGGGATTTGGAGGCTCAAGAGGAGATCCTGCATCAG
GAAGGCCATAGCATGGGTGTGTATGACATTGCCTTCCATCAAGATGGCTCTTTGGCTGGC
ACTGGGGGACTGGATGCATTTGGTCGAGTTTGGGACCTACGCACAGGACGTTGTATCATG
TTCTTAGAAGGCCACCTGAAAGAAATCTATGGAATAAATTTCTCCCCCAATGGCTATCAC
ATTGCAACCGGCAGTGGTGACAACACCTGCAAAGTGTGGGACCTCCGACAGCGGCGTTGC
GTCTACACCATCCCTGCTCATCAGAACTTAGTGACTGGTGTCAAGTTTGAGCCTATCCAT
GGGAACTTCTTGCTTACTGGTGCCTATGATAACACAGCCAAGATCTGGACGCACCCAGGC
TGGTCCCCGCTGAAGACTCTGGCTGGCCACGAAGGCAAAGTGATGGGCCTAGATATTTCT
TCCGATGGGCAGCTCATAGCCACTTGCTCATATGACAGGACCTTCAAGCTGTGGATGGCT
GAA

ORF Protein

Cloned ORF protein sequence for pFN21AB5305 Download

>KIBB5305  521 aa
MASSRASSTATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKDGLKAGIE
AGNINITSGEVFEIEEHISERQAEVLAEFERRKRARQINVSTDDSEVKACLRALGEPITL
FGEGPAERRERLRNILSVVGTDALKKTKKDDEKSKKSKEEYQQTWYHEGPNSLKVARLWI
ANYSLPRAMKRLEEARLHKEIPETTRTSQMQELHKSLRSLNNFCSQIGDDRPISYCHFSP
NSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAA
DGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWDLEAQEEILHQ
EGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYH
IATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPG
WSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5305 Download

>pFN21AB5305  6268 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTTCCTCGCGAGCCTCTTCCACGGCAACCAA
AACTAAAGCACCCGACGACTTAGTTGCTCCGGTCGTGAAGAAACCACACATCTATTATGG
AAGTTTGGAAGAGAAGGAGAGGGAGCGTCTGGCCAAAGGAGAGTCTGGGATTTTGGGGAA
AGACGGACTTAAAGCAGGGATCGAAGCTGGAAATATTAATATAACCTCTGGAGAAGTGTT
TGAAATTGAAGAGCATATCAGCGAGCGACAGGCAGAAGTATTGGCTGAGTTTGAGAGAAG
GAAGCGAGCCCGGCAGATCAATGTTTCCACAGATGACTCAGAGGTCAAAGCTTGCCTTAG
AGCCTTGGGGGAACCCATCACACTTTTTGGAGAGGGTCCTGCTGAAAGAAGAGAAAGGTT
AAGAAATATCCTCTCAGTTGTCGGTACTGATGCCTTGAAAAAGACCAAAAAGGATGATGA
GAAGTCTAAAAAGTCCAAAGAAGAGTATCAGCAAACCTGGTATCATGAAGGACCAAATAG
CTTGAAGGTGGCAAGACTATGGATTGCTAATTATTCGTTGCCCAGGGCAATGAAACGCTT
GGAAGAGGCCCGACTCCATAAGGAGATTCCTGAGACAACAAGGACCTCCCAGATGCAAGA
GCTGCACAAGTCTCTCCGGTCTTTGAATAATTTTTGCAGTCAGATTGGGGATGATCGGCC
TATCTCCTACTGTCACTTTAGTCCCAATTCCAAGATGCTGGCCACAGCTTGTTGGAGTGG
GCTTTGCAAGCTCTGGTCTGTTCCTGATTGCAACCTCCTTCACACTCTTCGAGGGCATAA
CACAAATGTAGGAGCAATTGTATTCCATCCCAAATCCACTGTCTCCTTGGACCCAAAAGA
TGTCAACCTGGCCTCTTGTGCGGCTGATGGCTCTGTGAAGCTTTGGAGTCTCGACAGTGA
TGAACCAGTGGCAGATATTGAAGGCCATACAGTGCGTGTGGCGCGGGTAATGTGGCATCC
TTCAGGACGTTTCCTGGGCACCACCTGCTATGACCGTTCATGGCGCTTATGGGATTTGGA
GGCTCAAGAGGAGATCCTGCATCAGGAAGGCCATAGCATGGGTGTGTATGACATTGCCTT
CCATCAAGATGGCTCTTTGGCTGGCACTGGGGGACTGGATGCATTTGGTCGAGTTTGGGA
CCTACGCACAGGACGTTGTATCATGTTCTTAGAAGGCCACCTGAAAGAAATCTATGGAAT
AAATTTCTCCCCCAATGGCTATCACATTGCAACCGGCAGTGGTGACAACACCTGCAAAGT
GTGGGACCTCCGACAGCGGCGTTGCGTCTACACCATCCCTGCTCATCAGAACTTAGTGAC
TGGTGTCAAGTTTGAGCCTATCCATGGGAACTTCTTGCTTACTGGTGCCTATGATAACAC
AGCCAAGATCTGGACGCACCCAGGCTGGTCCCCGCTGAAGACTCTGGCTGGCCACGAAGG
CAAAGTGATGGGCCTAGATATTTCTTCCGATGGGCAGCTCATAGCCACTTGCTCATATGA
CAGGACCTTCAAGCTGTGGATGGCTGAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT