FRK ORF clone pFN21AB5919

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Halotag ORF Clone pFN21AB5919

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Clone info.

Species

human

Clone Name

pFN21AB5919 Sequence (with vector) Restriction Map

Product ID

FHC08658

Flexi Clone PID

FXC08658

ORF Sequence

DNA sequence (1515 bp)
Protein sequence (505 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528388]

Gene

KIBB5919

Symbol

FRK

Alias : GTK, PTK5, RAK

Description

fyn-related Src family tyrosine kinase

Original Clone ID

cs00619

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1515 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 505 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5103.1 3.1e-89 100.0 FRK CCDS5094.1 1e-40 54.0 FYN CCDS54456.1 4.6e-39 51.0 HCK CCDS54455.1 4.6e-39 50.9 HCK CCDS54453.1 4.7e-39 51.0 HCK The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001452 45 55 PR00452 Src homology-3 domain IPR001452 59 74 PR00452 Src homology-3 domain IPR001452 76 85 PR00452 Src homology-3 domain IPR001452 96 108 PR00452 Src homology-3 domain IPR000980 116 130 PR00401 SH2 motif IPR000980 137 147 PR00401 SH2 motif IPR000980 149 160 PR00401 SH2 motif IPR000980 161 171 PR00401 SH2 motif IPR000980 182 196 PR00401 SH2 motif IPR001245 306 319 PR00109 Serine-threonine/tyrosine-protein kinase IPR001245 344 362 PR00109 Serine-threonine/tyrosine-protein kinase IPR001245 395 405 PR00109 Serine-threonine/tyrosine-protein kinase IPR001245 414 436 PR00109 Serine-threonine/tyrosine-protein kinase IPR001245 458 480 PR00109 Serine-threonine/tyrosine-protein kinase HMMPfam IPR001452 48 88 PF00018 Src homology-3 domain IPR000980 116 193 PF00017 SH2 motif IPR001245 234 486 PF07714 Serine-threonine/tyrosine-protein kinase HMMSmart IPR001452 45 109 SM00326 Src homology-3 domain IPR000980 114 199 SM00252 SH2 motif IPR002290 234 491 SM00220 Serine/threonine-protein kinase domain IPR020635 234 487 SM00219 Tyrosine-protein kinase ProfileScan IPR001452 42 110 PS50002 Src homology-3 domain IPR000980 116 208 PS50001 SH2 motif IPR000719 234 491 PS50011 Protein kinase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016866134 505 606573 100.0 100.0 XP_011533957 505 606573 100.0 100.0 XP_011533956 505 606573 100.0 100.0 XP_005266937 505 606573 100.0 100.0 XP_005266939 505 606573 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5919 Download

>KIBB5919  1515 bp
ATGAGCAACATCTGTCAGAGGCTCTGGGAGTACCTAGAACCCTATCTCCCCTGTTTGTCC
ACGGAGGCAGACAAGTCAACCGTGATTGAAAATCCAGGGGCCCTTTGCTCTCCCCAGTCA
CAGAGGCATGGCCACTACTTTGTGGCTTTGTTTGATTACCAGGCTCGGACTGCTGAGGAC
TTGAGCTTCCGAGCAGGTGACAAACTTCAAGTTCTGGACACTTTGCATGAGGGCTGGTGG
TTTGCCAGACACTTGGAGAAAAGACGAGATGGCTCCAGTCAGCAACTACAAGGCTATATT
CCTTCTAACTACGTGGCTGAGGACAGAAGCCTACAGGCAGAGCCGTGGTTCTTTGGAGCA
ATCGGAAGATCAGATGCAGAGAAACAACTATTATATTCAGAAAACAAGACCGGTTCCTTT
CTAATCAGAGAAAGTGAAAGCCAAAAAGGAGAATTCTCTCTTTCAGTTTTAGATGGAGCA
GTTGTAAAACACTACAGAATTAAAAGACTGGATGAAGGGGGATTTTTTCTCACGCGAAGA
AGAATCTTTTCAACACTGAACGAATTTGTGAGCCACTACACCAAGACAAGTGACGGCCTG
TGTGTCAAGCTGGGGAAACCATGCTTAAAGATCCAGGTCCCAGCTCCATTTGATTTGTCG
TATAAAACCGTGGACCAATGGGAGATAGACCGCAACTCCATACAGCTTCTGAAGCGATTG
GGATCTGGTCAGTTTGGCGAAGTATGGGAAGGTCTGTGGAACAATACCACTCCAGTAGCA
GTGAAAACATTAAAACCAGGTTCAATGGATCCAAATGACTTCCTGAGGGAGGCACAGATA
ATGAAGAACCTAAGACATCCAAAGCTTATCCAGCTTTATGCTGTTTGCACTTTAGAAGAT
CCAATTTATATTATTACAGAGTTGATGAGACATGGAAGTCTGCAAGAATATCTCCAAAAT
GACACTGGATCAAAAATCCATCTGACTCAACAGGTAGACATGGCGGCACAGGTTGCCTCT
GGAATGGCCTATCTGGAGTCTCGGAACTACATTCACAGAGATCTGGCTGCCAGAAATGTC
CTCGTTGGTGAACATAATATCTACAAAGTAGCAGATTTTGGACTTGCCAGAGTTTTTAAG
GTAGATAATGAAGACATCTATGAATCTAGACACGAAATAAAGCTGCCGGTGAAGTGGACT
GCGCCCGAAGCCATTCGTAGTAATAAATTCAGCATTAAGTCCGATGTATGGTCATTTGGA
ATCCTTCTTTATGAAATCATTACTTATGGCAAAATGCCTTACAGTGGTATGACAGGTGCC
CAGGTAATCCAGATGTTGGCTCAAAACTATAGACTTCCGCAACCATCCAACTGTCCACAG
CAATTTTACAACATCATGTTGGAGTGCTGGAATGCAGAGCCTAAGGAACGACCTACATTT
GAGACACTGCGTTGGAAACTTGAAGACTATTTTGAAACAGACTCTTCATATTCAGATGCA
AATAACTTCATAAGA

ORF Protein

Cloned ORF protein sequence for pFN21AB5919 Download

>KIBB5919  505 aa
MSNICQRLWEYLEPYLPCLSTEADKSTVIENPGALCSPQSQRHGHYFVALFDYQARTAED
LSFRAGDKLQVLDTLHEGWWFARHLEKRRDGSSQQLQGYIPSNYVAEDRSLQAEPWFFGA
IGRSDAEKQLLYSENKTGSFLIRESESQKGEFSLSVLDGAVVKHYRIKRLDEGGFFLTRR
RIFSTLNEFVSHYTKTSDGLCVKLGKPCLKIQVPAPFDLSYKTVDQWEIDRNSIQLLKRL
GSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPNDFLREAQIMKNLRHPKLIQLYAVCTLED
PIYIITELMRHGSLQEYLQNDTGSKIHLTQQVDMAAQVASGMAYLESRNYIHRDLAARNV
LVGEHNIYKVADFGLARVFKVDNEDIYESRHEIKLPVKWTAPEAIRSNKFSIKSDVWSFG
ILLYEIITYGKMPYSGMTGAQVIQMLAQNYRLPQPSNCPQQFYNIMLECWNAEPKERPTF
ETLRWKLEDYFETDSSYSDANNFIR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5919 Download

>pFN21AB5919  6220 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCAACATCTGTCAGAGGCTCTGGGAGTACCT
AGAACCCTATCTCCCCTGTTTGTCCACGGAGGCAGACAAGTCAACCGTGATTGAAAATCC
AGGGGCCCTTTGCTCTCCCCAGTCACAGAGGCATGGCCACTACTTTGTGGCTTTGTTTGA
TTACCAGGCTCGGACTGCTGAGGACTTGAGCTTCCGAGCAGGTGACAAACTTCAAGTTCT
GGACACTTTGCATGAGGGCTGGTGGTTTGCCAGACACTTGGAGAAAAGACGAGATGGCTC
CAGTCAGCAACTACAAGGCTATATTCCTTCTAACTACGTGGCTGAGGACAGAAGCCTACA
GGCAGAGCCGTGGTTCTTTGGAGCAATCGGAAGATCAGATGCAGAGAAACAACTATTATA
TTCAGAAAACAAGACCGGTTCCTTTCTAATCAGAGAAAGTGAAAGCCAAAAAGGAGAATT
CTCTCTTTCAGTTTTAGATGGAGCAGTTGTAAAACACTACAGAATTAAAAGACTGGATGA
AGGGGGATTTTTTCTCACGCGAAGAAGAATCTTTTCAACACTGAACGAATTTGTGAGCCA
CTACACCAAGACAAGTGACGGCCTGTGTGTCAAGCTGGGGAAACCATGCTTAAAGATCCA
GGTCCCAGCTCCATTTGATTTGTCGTATAAAACCGTGGACCAATGGGAGATAGACCGCAA
CTCCATACAGCTTCTGAAGCGATTGGGATCTGGTCAGTTTGGCGAAGTATGGGAAGGTCT
GTGGAACAATACCACTCCAGTAGCAGTGAAAACATTAAAACCAGGTTCAATGGATCCAAA
TGACTTCCTGAGGGAGGCACAGATAATGAAGAACCTAAGACATCCAAAGCTTATCCAGCT
TTATGCTGTTTGCACTTTAGAAGATCCAATTTATATTATTACAGAGTTGATGAGACATGG
AAGTCTGCAAGAATATCTCCAAAATGACACTGGATCAAAAATCCATCTGACTCAACAGGT
AGACATGGCGGCACAGGTTGCCTCTGGAATGGCCTATCTGGAGTCTCGGAACTACATTCA
CAGAGATCTGGCTGCCAGAAATGTCCTCGTTGGTGAACATAATATCTACAAAGTAGCAGA
TTTTGGACTTGCCAGAGTTTTTAAGGTAGATAATGAAGACATCTATGAATCTAGACACGA
AATAAAGCTGCCGGTGAAGTGGACTGCGCCCGAAGCCATTCGTAGTAATAAATTCAGCAT
TAAGTCCGATGTATGGTCATTTGGAATCCTTCTTTATGAAATCATTACTTATGGCAAAAT
GCCTTACAGTGGTATGACAGGTGCCCAGGTAATCCAGATGTTGGCTCAAAACTATAGACT
TCCGCAACCATCCAACTGTCCACAGCAATTTTACAACATCATGTTGGAGTGCTGGAATGC
AGAGCCTAAGGAACGACCTACATTTGAGACACTGCGTTGGAAACTTGAAGACTATTTTGA
AACAGACTCTTCATATTCAGATGCAAATAACTTCATAAGAGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT