NUB1 ORF clone pFN21AB6106

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB6106

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Clone info.

Species

human

Clone Name

pFN21AB6106 Sequence (with vector) Restriction Map

Product ID

FHC12647

ORF Sequence

DNA sequence (1803 bp)
Protein sequence (601 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB6106

Symbol

NUB1

Alias : BS4, NUB1L, NYREN18

Description

negative regulator of ubiquitin-like proteins 1

Original Clone ID

cs00706

Note

We obtained this clone using the E.coli strain JM109.

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1803 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 601 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS47751.2 1.6e-201 100.0 NUB1 CCDS59089.1 6e-151 97.7 NUB1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000449 375 410 PF00627 Ubiquitin-associated/translation elongation factor EF1B IPR000449 477 512 PF00627 Ubiquitin-associated/translation elongation factor EF1B HMMSmart IPR015940 376 413 SM00165 Ubiquitin-associated/translation elongation factor EF1B IPR015940 477 514 SM00165 Ubiquitin-associated/translation elongation factor EF1B ProfileScan IPR015940 374 414 PS50030 Ubiquitin-associated/translation elongation factor EF1B IPR015940 475 515 PS50030 Ubiquitin-associated/translation elongation factor EF1B
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016867795 601 607981 100.0 100.0 NP_057202 625 607981 100.0 100.0 XP_016867794 624 607981 99.8 100.0 XP_005250068 615 607981 97.7 100.0 NP_001230280 639 607981 97.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6106 Download

>KIBB6106  1803 bp
ATGGCACAAAAGAAATATCTTCAAGCAAAATTGACCCAGTTTTTAAGGGAAGACAGGATT
CAACTTTGGAAACCTCCATATACAGATGAAAATAAAAAAGTTGGTTTGGCATTAAAGGAC
CTTGCTAAGCAGTACTCTGACAGACTAGAATGCTGTGAAAATGAAGTAGAAAAGGTAATA
GAAGAAATACGTTGCAAGGCAATTGAGCGTGGAACAGGAAATGACAATTATAGAACAACG
GGAATTGCTACAATCGAGGTGTTTTTACCACCAAGACTAAAAAAAGATAGGAAAAACTTG
TTGGAGACCCGATTGCACATCACTGGCAGAGAACTGAGGTCCAAAATAGCTGAAACCTTT
GGACTTCAAGAAAATTATATCAAAATTGTCATAAATAAGAAGCAACTACAACTAGGGAAA
ACCCTTGAAGAACAAGGCGTGGCTCACAATGTGAAAGCGATGGTGCTTGAACTAAAACAA
TCTGAAGAGGACGCGAGGAAAAACTTCCAGTTAGAGGAAGAGGAGCAAAATGAGGCCAAA
CTCAAAGAAAAACAAATTCAGAGGACCAAGAGAGGACTAGAAATACTGGCAAAGAGAGCA
GCAGAGACAGTGGTGGATCCAGAAATGACACCGTACTTAGACATAGCTAACCAGACAGGC
AGATCAATCAGAATTCCCCCATCAGAAAGAAAAGCCCTTATGTTAGCTATGGGATATCAT
GAGAAGGGCAGAGCTTTCCTGAAAAGAAAAGAATATGGAATAGCCTTGCCATGTCTGTTG
GACGCTGACAAATATTTCTGTGAGTGTTGCAGAGAGCTGCTGGACACAGTGGATAACTAC
GCCGTCCTCCAGCTGGATATAGTGTGGTGTTACTTCCGCCTGGAACAGCTGGAATGCCTT
GATGATGCAGAAAAAAAATTAAACTTGGCCCAGAAATGCTTTAAAAATTGTTACGGAGAA
AATCATCAGAGACTGGTCCACATAAAAGGAAATTGTGGGAAAGAGAAGGTACTGTTTCTA
AGACTCTACTTACTTCAAGGGATCCGAAACTATCACAGTGGAAATGATGTAGAGGCTTAT
GAGTATCTTAACAAGGCACGTCAGCTCTTTAAAGAGCTATATATTGATCCATCAAAAGTG
GACAATTTGTTGCAGTTGGGGTTTACTGCCCAGGAAGCCCGGCTTGGCCTGAGGGCGTGT
GATGGGAACGTGGATCATGCGGCCACTCATATTACCAACCGCAGAGAGGAACTGGCCCAA
ATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCGCCTCGAGAACATCAGGTTTCTGAAA
GGGATGGGCTACTCCACGCACGCGGCCCAGCAGATTCTGCTCAGCAATCCTCAGATGTGG
TGGTTAAATGATTCCAATCCTGAAACCGACAACCGTCAAGAAAGTCCTTCCCAGGAAAAC
ATTGACCGATTGGTGTACATGGGTTTTGATGCACTCGTGGCCGAAGCTGCGCTGAGAGTG
TTCAGAGGCAACGTCCAGCTGGCCGCCCAGACCCTTGCTCACAACGGAGGAAGCCTGCCT
CCCGAGCTGCCGCTGTCGCCAGAAGACTCTTTGTCCCCGCCAGCCACGTCCCCTTCTGAC
TCCGCAGGAACCTCTAGTGCCTCAACAGACGAAGACATGGAGACAGAGGCCGTCAATGAG
ATACTGGAAGACATTCCAGAGCATGAGGAAGACTATCTTGACTCAACTCTGGAAGATGAA
GAAATTATTATTGCAGAGTACCTATCCTATGTAGAAAATAGGAAGTCAGCAACAAAGAAA
AAC

ORF Protein

Cloned ORF protein sequence for pFN21AB6106 Download

>KIBB6106  601 aa
MAQKKYLQAKLTQFLREDRIQLWKPPYTDENKKVGLALKDLAKQYSDRLECCENEVEKVI
EEIRCKAIERGTGNDNYRTTGIATIEVFLPPRLKKDRKNLLETRLHITGRELRSKIAETF
GLQENYIKIVINKKQLQLGKTLEEQGVAHNVKAMVLELKQSEEDARKNFQLEEEEQNEAK
LKEKQIQRTKRGLEILAKRAAETVVDPEMTPYLDIANQTGRSIRIPPSERKALMLAMGYH
EKGRAFLKRKEYGIALPCLLDADKYFCECCRELLDTVDNYAVLQLDIVWCYFRLEQLECL
DDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQGIRNYHSGNDVEAY
EYLNKARQLFKELYIDPSKVDNLLQLGFTAQEARLGLRACDGNVDHAATHITNRREELAQ
IRKEEKEKKRRRLENIRFLKGMGYSTHAAQQILLSNPQMWWLNDSNPETDNRQESPSQEN
IDRLVYMGFDALVAEAALRVFRGNVQLAAQTLAHNGGSLPPELPLSPEDSLSPPATSPSD
SAGTSSASTDEDMETEAVNEILEDIPEHEEDYLDSTLEDEEIIIAEYLSYVENRKSATKK
N

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6106 Download

>pFN21AB6106  6508 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCACAAAAGAAATATCTTCAAGCAAAATTGAC
CCAGTTTTTAAGGGAAGACAGGATTCAACTTTGGAAACCTCCATATACAGATGAAAATAA
AAAAGTTGGTTTGGCATTAAAGGACCTTGCTAAGCAGTACTCTGACAGACTAGAATGCTG
TGAAAATGAAGTAGAAAAGGTAATAGAAGAAATACGTTGCAAGGCAATTGAGCGTGGAAC
AGGAAATGACAATTATAGAACAACGGGAATTGCTACAATCGAGGTGTTTTTACCACCAAG
ACTAAAAAAAGATAGGAAAAACTTGTTGGAGACCCGATTGCACATCACTGGCAGAGAACT
GAGGTCCAAAATAGCTGAAACCTTTGGACTTCAAGAAAATTATATCAAAATTGTCATAAA
TAAGAAGCAACTACAACTAGGGAAAACCCTTGAAGAACAAGGCGTGGCTCACAATGTGAA
AGCGATGGTGCTTGAACTAAAACAATCTGAAGAGGACGCGAGGAAAAACTTCCAGTTAGA
GGAAGAGGAGCAAAATGAGGCCAAACTCAAAGAAAAACAAATTCAGAGGACCAAGAGAGG
ACTAGAAATACTGGCAAAGAGAGCAGCAGAGACAGTGGTGGATCCAGAAATGACACCGTA
CTTAGACATAGCTAACCAGACAGGCAGATCAATCAGAATTCCCCCATCAGAAAGAAAAGC
CCTTATGTTAGCTATGGGATATCATGAGAAGGGCAGAGCTTTCCTGAAAAGAAAAGAATA
TGGAATAGCCTTGCCATGTCTGTTGGACGCTGACAAATATTTCTGTGAGTGTTGCAGAGA
GCTGCTGGACACAGTGGATAACTACGCCGTCCTCCAGCTGGATATAGTGTGGTGTTACTT
CCGCCTGGAACAGCTGGAATGCCTTGATGATGCAGAAAAAAAATTAAACTTGGCCCAGAA
ATGCTTTAAAAATTGTTACGGAGAAAATCATCAGAGACTGGTCCACATAAAAGGAAATTG
TGGGAAAGAGAAGGTACTGTTTCTAAGACTCTACTTACTTCAAGGGATCCGAAACTATCA
CAGTGGAAATGATGTAGAGGCTTATGAGTATCTTAACAAGGCACGTCAGCTCTTTAAAGA
GCTATATATTGATCCATCAAAAGTGGACAATTTGTTGCAGTTGGGGTTTACTGCCCAGGA
AGCCCGGCTTGGCCTGAGGGCGTGTGATGGGAACGTGGATCATGCGGCCACTCATATTAC
CAACCGCAGAGAGGAACTGGCCCAAATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCG
CCTCGAGAACATCAGGTTTCTGAAAGGGATGGGCTACTCCACGCACGCGGCCCAGCAGAT
TCTGCTCAGCAATCCTCAGATGTGGTGGTTAAATGATTCCAATCCTGAAACCGACAACCG
TCAAGAAAGTCCTTCCCAGGAAAACATTGACCGATTGGTGTACATGGGTTTTGATGCACT
CGTGGCCGAAGCTGCGCTGAGAGTGTTCAGAGGCAACGTCCAGCTGGCCGCCCAGACCCT
TGCTCACAACGGAGGAAGCCTGCCTCCCGAGCTGCCGCTGTCGCCAGAAGACTCTTTGTC
CCCGCCAGCCACGTCCCCTTCTGACTCCGCAGGAACCTCTAGTGCCTCAACAGACGAAGA
CATGGAGACAGAGGCCGTCAATGAGATACTGGAAGACATTCCAGAGCATGAGGAAGACTA
TCTTGACTCAACTCTGGAAGATGAAGAAATTATTATTGCAGAGTACCTATCCTATGTAGA
AAATAGGAAGTCAGCAACAAAGAAAAACGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT