PRKG1 ORF clone pFN21AB6261

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB6261

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Clone info.

Species

human

Clone Name

pFN21AB6261 Sequence (with vector) Restriction Map

Product ID

FHC23849

ORF Sequence

DNA sequence (2058 bp)
Protein sequence (686 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590766]

Gene

KIBB6261

Symbol

PRKG1

Alias : AAT8, PKG, PKG1, PRKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha

Description

protein kinase, cGMP-dependent, type I, transcript variant 2

Original Clone ID

cs00861

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2058 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 686 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7244.1 4.6e-156 100.0 PRKG1 CCDS44399.1 2.5e-135 91.8 PRKG1 CCDS64005.1 2.2e-72 49.2 PRKG2 CCDS75150.1 6.2e-48 64.7 PRKG2 CCDS3589.1 1.1e-47 49.6 PRKG2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002374 242 256 PR00104 cGMP-dependent kinase IPR002374 277 286 PR00104 cGMP-dependent kinase IPR002374 295 302 PR00104 cGMP-dependent kinase IPR002374 313 322 PR00104 cGMP-dependent kinase IPR002374 326 335 PR00104 cGMP-dependent kinase IPR002374 336 350 PR00104 cGMP-dependent kinase HMMPfam IPR000595 137 218 PF00027 Cyclic nucleotide-binding domain IPR000595 256 341 PF00027 Cyclic nucleotide-binding domain IPR017442 376 634 PF00069 Serine/threonine-protein kinase-like domain HMMSmart IPR000595 118 231 SM00100 Cyclic nucleotide-binding domain IPR000595 236 358 SM00100 Cyclic nucleotide-binding domain IPR002290 375 634 SM00220 Serine/threonine-protein kinase domain IPR020635 375 628 SM00219 Tyrosine-protein kinase IPR000961 635 686 SM00133 AGC-kinase ProfileScan IPR000595 118 233 PS50042 Cyclic nucleotide-binding domain IPR000595 236 357 PS50042 Cyclic nucleotide-binding domain IPR000719 375 634 PS50011 Protein kinase IPR000961 635 686 PS51285 AGC-kinase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006249 686 176894,615436 100.0 100.0 NP_001091982 671 176894,615436 91.8 97.5 XP_016871901 591 176894,615436 99.8 85.1 XP_016871902 585 176894,615436 99.8 85.0 NP_001269414 733 601591 49.2 99.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6261 Download

>KIBB6261  2058 bp
ATGGGCACCTTGCGGGATTTACAGTACGCGCTCCAGGAGAAGATCGAGGAGCTGAGGCAG
CGGGATGCTCTCATCGACGAGCTGGAGCTGGAGTTGGATCAGAAGGACGAACTGATCCAG
AAGCTGCAGAACGAGCTGGACAAGTACCGCTCGGTGATCCGACCAGCCACCCAGCAGGCG
CAGAAGCAGAGCGCGAGCACCTTGCAGGGCGAGCCGCGCACCAAGCGGCAGGCGATCTCC
GCCGAGCCCACCGCCTTCGACATCCAGGATCTCAGCCATGTGACCCTGCCCTTCTACCCC
AAGAGCCCACAGTCCAAGGATCTTATAAAGGAAGCTATCCTTGACAATGACTTTATGAAG
AACTTGGAGCTGTCGCAGATCCAGGAGATTGTGGATTGTATGTACCCGGTGGAGTATGGC
AAGGACAGTTGCATCATCAAAGAAGGAGACGTGGGGTCACTGGTGTATGTCATGGAAGAT
GGTAAGGTTGAAGTTACAAAAGAAGGTGTGAAGTTGTGTACCATGGGTCCAGGAAAAGTG
TTTGGGGAATTGGCTATTCTTTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTA
AATGTAAAACTCTGGGCCATTGATCGACAATGTTTTCAAACAATAATGATGAGGACAGGA
CTCATCAAGCATACCGAGTATATGGAATTTTTAAAAAGCGTTCCAACATTCCAGAGCCTT
CCTGAAGAGATCCTCAGCAAGCTTGCTGATGTCCTTGAAGAGACCCACTATGAAAATGGA
GAATATATTATCAGGCAAGGTGCAAGAGGGGACACCTTCTTTATCATCAGCAAAGGAACG
GTAAATGTCACTCGTGAAGACTCACCGAGTGAAGACCCAGTCTTTCTTAGAACTTTAGGA
AAAGGAGACTGGTTTGGAGAGAAAGCCTTGCAGGGGGAAGATGTGAGAACAGCAAACGTA
ATTGCTGCAGAAGCTGTAACCTGCCTTGTGATTGACAGAGACTCTTTTAAACATTTGATT
GGAGGGCTGGATGATGTTTCTAATAAAGCATATGAAGATGCAGAAGCTAAAGCAAAATAT
GAAGCTGAAGCGGCTTTCTTCGCCAACCTGAAGCTGTCTGATTTCAACATCATTGATACC
CTTGGAGTTGGAGGTTTCGGACGAGTAGAACTGGTCCAGTTGAAAAGTGAAGAATCCAAA
ACGTTTGCAATGAAGATTCTCAAGAAACGTCACATTGTGGACACAAGACAGCAGGAGCAC
ATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCCGATTTCATAGTGAGACTGTAC
AGAACATTTAAGGACAGCAAATATTTGTATATGTTGATGGAAGCTTGTCTAGGTGGAGAG
CTCTGGACCATTCTCAGGGATAGAGGTTCGTTTGAAGATTCTACAACCAGATTTTACACA
GCATGTGTGGTAGAAGCTTTTGCCTATCTGCATTCCAAAGGAATCATTTACAGGGACCTC
AAGCCAGAAAATCTCATCCTAGATCACCGAGGTTATGCCAAACTGGTTGATTTTGGCTTT
GCAAAGAAAATAGGATTTGGAAAGAAAACATGGACTTTTTGTGGGACTCCAGAGTATGTA
GCCCCAGAGATCATCCTGAACAAAGGCCATGACATTTCAGCCGACTACTGGTCACTGGGA
ATCCTAATGTATGAACTCCTGACTGGCAGCCCACCTTTCTCAGGCCCAGATCCTATGAAA
ACCTATAACATCATATTGAGGGGGATTGACATGATAGAATTTCCAAAGAAGATTGCCAAA
AATGCTGCTAATTTAATTAAAAAACTATGCAGGGACAATCCATCAGAAAGATTAGGGAAT
TTGAAAAATGGAGTAAAAGACATTCAAAAGCACAAATGGTTTGAGGGCTTTAACTGGGAA
GGCTTAAGAAAAGGTACCTTGACACCTCCTATAATACCAAGTGTTGCATCACCCACAGAC
ACAAGTAATTTTGACAGTTTCCCTGAGGACAACGATGAACCACCACCTGATGACAACTCA
GGATGGGATATAGACTTC

ORF Protein

Cloned ORF protein sequence for pFN21AB6261 Download

>KIBB6261  686 aa
MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQA
QKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMK
NLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKV
FGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSL
PEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLG
KGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKY
EAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEH
IRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYT
ACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYV
APEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAK
NAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTD
TSNFDSFPEDNDEPPPDDNSGWDIDF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6261 Download

>pFN21AB6261  6763 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCACCTTGCGGGATTTACAGTACGCGCTCCA
GGAGAAGATCGAGGAGCTGAGGCAGCGGGATGCTCTCATCGACGAGCTGGAGCTGGAGTT
GGATCAGAAGGACGAACTGATCCAGAAGCTGCAGAACGAGCTGGACAAGTACCGCTCGGT
GATCCGACCAGCCACCCAGCAGGCGCAGAAGCAGAGCGCGAGCACCTTGCAGGGCGAGCC
GCGCACCAAGCGGCAGGCGATCTCCGCCGAGCCCACCGCCTTCGACATCCAGGATCTCAG
CCATGTGACCCTGCCCTTCTACCCCAAGAGCCCACAGTCCAAGGATCTTATAAAGGAAGC
TATCCTTGACAATGACTTTATGAAGAACTTGGAGCTGTCGCAGATCCAGGAGATTGTGGA
TTGTATGTACCCGGTGGAGTATGGCAAGGACAGTTGCATCATCAAAGAAGGAGACGTGGG
GTCACTGGTGTATGTCATGGAAGATGGTAAGGTTGAAGTTACAAAAGAAGGTGTGAAGTT
GTGTACCATGGGTCCAGGAAAAGTGTTTGGGGAATTGGCTATTCTTTACAACTGTACCCG
GACAGCGACCGTCAAGACTCTTGTAAATGTAAAACTCTGGGCCATTGATCGACAATGTTT
TCAAACAATAATGATGAGGACAGGACTCATCAAGCATACCGAGTATATGGAATTTTTAAA
AAGCGTTCCAACATTCCAGAGCCTTCCTGAAGAGATCCTCAGCAAGCTTGCTGATGTCCT
TGAAGAGACCCACTATGAAAATGGAGAATATATTATCAGGCAAGGTGCAAGAGGGGACAC
CTTCTTTATCATCAGCAAAGGAACGGTAAATGTCACTCGTGAAGACTCACCGAGTGAAGA
CCCAGTCTTTCTTAGAACTTTAGGAAAAGGAGACTGGTTTGGAGAGAAAGCCTTGCAGGG
GGAAGATGTGAGAACAGCAAACGTAATTGCTGCAGAAGCTGTAACCTGCCTTGTGATTGA
CAGAGACTCTTTTAAACATTTGATTGGAGGGCTGGATGATGTTTCTAATAAAGCATATGA
AGATGCAGAAGCTAAAGCAAAATATGAAGCTGAAGCGGCTTTCTTCGCCAACCTGAAGCT
GTCTGATTTCAACATCATTGATACCCTTGGAGTTGGAGGTTTCGGACGAGTAGAACTGGT
CCAGTTGAAAAGTGAAGAATCCAAAACGTTTGCAATGAAGATTCTCAAGAAACGTCACAT
TGTGGACACAAGACAGCAGGAGCACATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCA
TTCCGATTTCATAGTGAGACTGTACAGAACATTTAAGGACAGCAAATATTTGTATATGTT
GATGGAAGCTTGTCTAGGTGGAGAGCTCTGGACCATTCTCAGGGATAGAGGTTCGTTTGA
AGATTCTACAACCAGATTTTACACAGCATGTGTGGTAGAAGCTTTTGCCTATCTGCATTC
CAAAGGAATCATTTACAGGGACCTCAAGCCAGAAAATCTCATCCTAGATCACCGAGGTTA
TGCCAAACTGGTTGATTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGAAAACATGGAC
TTTTTGTGGGACTCCAGAGTATGTAGCCCCAGAGATCATCCTGAACAAAGGCCATGACAT
TTCAGCCGACTACTGGTCACTGGGAATCCTAATGTATGAACTCCTGACTGGCAGCCCACC
TTTCTCAGGCCCAGATCCTATGAAAACCTATAACATCATATTGAGGGGGATTGACATGAT
AGAATTTCCAAAGAAGATTGCCAAAAATGCTGCTAATTTAATTAAAAAACTATGCAGGGA
CAATCCATCAGAAAGATTAGGGAATTTGAAAAATGGAGTAAAAGACATTCAAAAGCACAA
ATGGTTTGAGGGCTTTAACTGGGAAGGCTTAAGAAAAGGTACCTTGACACCTCCTATAAT
ACCAAGTGTTGCATCACCCACAGACACAAGTAATTTTGACAGTTTCCCTGAGGACAACGA
TGAACCACCACCTGATGACAACTCAGGATGGGATATAGACTTCGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT