ZNF433 ORF clone pFN21AB7876

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Halotag ORF Clone pFN21AB7876

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Clone info.

Species

human

Clone Name

pFN21AB7876 Sequence (with vector) Restriction Map

Product ID

FHC07472

Flexi Clone PID

FXC07472

ORF Sequence

DNA sequence (1914 bp)
Protein sequence (638 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464397]

Gene

KIBB7876

Symbol

ZNF433

Description

zinc finger protein 433

Original Clone ID

cs00376

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1914 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 638 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS77240.1 4.3e-149 100.0 ZNF433 CCDS45983.1 4.4e-149 100.0 ZNF433 CCDS32915.1 1.1e-89 61.9 ZNF700 CCDS74289.1 1.1e-89 61.9 ZNF700 CCDS42504.1 6.1e-87 62.3 ZNF709 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007087 135 157 PF00096 Zinc finger IPR007087 191 213 PF00096 Zinc finger IPR007087 331 353 PF00096 Zinc finger IPR007087 387 409 PF00096 Zinc finger IPR007087 499 521 PF00096 Zinc finger HMMSmart IPR015880 107 129 SM00355 Zinc finger IPR015880 135 157 SM00355 Zinc finger IPR015880 163 185 SM00355 Zinc finger IPR015880 191 213 SM00355 Zinc finger IPR015880 219 241 SM00355 Zinc finger IPR015880 247 269 SM00355 Zinc finger IPR015880 275 297 SM00355 Zinc finger IPR015880 303 325 SM00355 Zinc finger IPR015880 331 353 SM00355 Zinc finger IPR015880 359 381 SM00355 Zinc finger IPR015880 387 409 SM00355 Zinc finger IPR015880 415 437 SM00355 Zinc finger IPR015880 443 465 SM00355 Zinc finger IPR015880 471 493 SM00355 Zinc finger IPR015880 499 521 SM00355 Zinc finger IPR015880 527 549 SM00355 Zinc finger IPR015880 555 577 SM00355 Zinc finger IPR015880 583 605 SM00355 Zinc finger IPR015880 611 633 SM00355 Zinc finger ProfileScan IPR007087 107 134 PS50157 Zinc finger IPR007087 135 162 PS50157 Zinc finger IPR007087 163 190 PS50157 Zinc finger IPR007087 191 218 PS50157 Zinc finger IPR007087 219 246 PS50157 Zinc finger IPR007087 247 274 PS50157 Zinc finger IPR007087 275 302 PS50157 Zinc finger IPR007087 303 330 PS50157 Zinc finger IPR007087 331 358 PS50157 Zinc finger IPR007087 359 386 PS50157 Zinc finger IPR007087 387 414 PS50157 Zinc finger IPR007087 415 442 PS50157 Zinc finger IPR007087 443 470 PS50157 Zinc finger IPR007087 471 498 PS50157 Zinc finger IPR007087 499 526 PS50157 Zinc finger IPR007087 527 554 PS50157 Zinc finger IPR007087 555 582 PS50157 Zinc finger IPR007087 583 610 PS50157 Zinc finger IPR007087 611 638 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005806 671 606697 57.2 99.2 XP_011525924 639 606697 57.1 99.2 XP_016881629 673 606697 57.1 99.2 XP_011525925 718 606697 57.1 99.2 XP_016881628 685 606697 57.9 94.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7876 Download

>KIBB7876  1914 bp
ATGCAAGAAACCTTCAGGAACCTGGCCTCTATAGGGAAAAAATGGAAACCCCAGAACATA
TATGTAGAGTACGAAAATCTAAGGAGAAACCTAAGAATTGTGGGAGAGAGACTCTTTGAA
AGTAAAGAAGGTCATCAGCATGGAGAAATTTTGACCCAGGTTCCAGATGACATGCTGAAG
AAAACAACTACTGGAGTAAAATCATGCGAAAGCAGTGTGTATGGAGAAGTAGGCAGTGCT
CATTCATCTCTTAATAGGCACATCAGAGATGACACTGGACACAAGGCATATGAGTATCAA
GAATATGGACAGAAACCATATAAATGTAAATACTGTAAAAAACCTTTCAACTGTCTCTCC
TCTGTTCAGACACATGAAAGGGCTCATAGTGGAAGGAAACTCTATGTTTGTGAGGAATGC
GGAAAAACATTTATTTCCCATTCAAACCTTCAAAGACACAGGATAATGCACCGTGGAGAT
GGACCTTATAAGTGTAAATTTTGTGGGAAAGCCTTGATGTTTCTCAGTTTGTATCTTATC
CACAAACGAACTCACACTGGAGAGAAACCATATCAATGTAAACAGTGTGGTAAAGCCTTT
AGTCATTCTAGTAGCCTTCGAATACATGAAAGAACTCACACTGGGGAGAAGCCTTATAAA
TGTAATGAATGTGGGAAAGCATTCCATAGTTCCACATGCCTTCATGCTCATAAAAGAACT
CACACTGGGGAGAAGCCATATGAATGTAAACAGTGTGGGAAAGCCTTCAGCTCTTCCCAT
TCCTTTCAAATACATGAAAGAACTCACACGGGGGAGAAGCCATATGAATGTAAGGAATGT
GGAAAAGCATTCAAGTGTCCCAGTTCTGTTCGCAGACATGAAAGAACCCACTCTAGGAAA
AAACCCTATGAATGTAAACATTGTGGGAAAGTATTATCTTATCTTACCAGCTTTCAAAAC
CACTTGGGAATGCACACTGGAGAGATATCTCATAAATGTAAGATATGTGGGAAAGCCTTT
TATTCTCCCAGTTCACTTCAAACACATGAAAAAACTCACACTGGAGAGAAACCCTATAAA
TGCAACCAATGTGGTAAAGCCTTTAATTCTTCCAGTTCCTTCCGATATCATGAAAGAACT
CACACTGGAGAGAAACCTTACGAGTGTAAGCAATGTGGGAAAGCCTTCAGATCTGCCTCA
CTCCTTCAAACACATGGTAGGACTCACACGGGAGAGAAACCCTATGCATGTAAGGAATGT
GGAAAACCATTTAGTAATTTCTCTTTCTTTCAAATACATGAAAGGATGCACAGAGAAGAG
AAGCCGTATGAATGTAAGGGTTATGGGAAAACATTCAGTTTGCCCAGTTTATTTCATAGA
CATGAAAGGACTCACACTGGAGGAAAAACCTATGAATGCAAGCAGTGTGGCAGATCCTTC
AACTGTTCGAGCTCCTTTCGATATCATGGAAGGACTCACACTGGAGAGAAACCCTATGAA
TGCAAGCAATGTGGAAAAGCCTTCAGATCTGCCTCACAGCTTCAAATTCATGGAAGGACT
CACACTGGAGAGAAACCTTATGAATGTAAGCAGTGTGGGAAAGCCTTTGGATCTGCCTCA
CACCTTCAAATGCATGGAAGGACTCACACTGGAGAGAAACCCTATGAATGTAAGCAGTGT
GGGAAGTCTTTTGGATGTGCCTCGCGACTTCAAATGCATGGAAGGACTCACACTGGAGAG
AAACCGTATAAATGTAAGCAATGTGGGAAAGCTTTTGGATGTCCCTCAAACCTTCGAAGG
CATGGAAGGACTCACACTGGAGAGAAACCCTATAAATGTAACCAATGTGGTAAAGTCTTT
AGATGTTCTTCACAACTTCAAGTGCATGGAAGGGCTCACTGCATAGACACCCCA

ORF Protein

Cloned ORF protein sequence for pFN21AB7876 Download

>KIBB7876  638 aa
MQETFRNLASIGKKWKPQNIYVEYENLRRNLRIVGERLFESKEGHQHGEILTQVPDDMLK
KTTTGVKSCESSVYGEVGSAHSSLNRHIRDDTGHKAYEYQEYGQKPYKCKYCKKPFNCLS
SVQTHERAHSGRKLYVCEECGKTFISHSNLQRHRIMHRGDGPYKCKFCGKALMFLSLYLI
HKRTHTGEKPYQCKQCGKAFSHSSSLRIHERTHTGEKPYKCNECGKAFHSSTCLHAHKRT
HTGEKPYECKQCGKAFSSSHSFQIHERTHTGEKPYECKECGKAFKCPSSVRRHERTHSRK
KPYECKHCGKVLSYLTSFQNHLGMHTGEISHKCKICGKAFYSPSSLQTHEKTHTGEKPYK
CNQCGKAFNSSSSFRYHERTHTGEKPYECKQCGKAFRSASLLQTHGRTHTGEKPYACKEC
GKPFSNFSFFQIHERMHREEKPYECKGYGKTFSLPSLFHRHERTHTGGKTYECKQCGRSF
NCSSSFRYHGRTHTGEKPYECKQCGKAFRSASQLQIHGRTHTGEKPYECKQCGKAFGSAS
HLQMHGRTHTGEKPYECKQCGKSFGCASRLQMHGRTHTGEKPYKCKQCGKAFGCPSNLRR
HGRTHTGEKPYKCNQCGKVFRCSSQLQVHGRAHCIDTP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7876 Download

>pFN21AB7876  6619 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCAAGAAACCTTCAGGAACCTGGCCTCTATAGG
GAAAAAATGGAAACCCCAGAACATATATGTAGAGTACGAAAATCTAAGGAGAAACCTAAG
AATTGTGGGAGAGAGACTCTTTGAAAGTAAAGAAGGTCATCAGCATGGAGAAATTTTGAC
CCAGGTTCCAGATGACATGCTGAAGAAAACAACTACTGGAGTAAAATCATGCGAAAGCAG
TGTGTATGGAGAAGTAGGCAGTGCTCATTCATCTCTTAATAGGCACATCAGAGATGACAC
TGGACACAAGGCATATGAGTATCAAGAATATGGACAGAAACCATATAAATGTAAATACTG
TAAAAAACCTTTCAACTGTCTCTCCTCTGTTCAGACACATGAAAGGGCTCATAGTGGAAG
GAAACTCTATGTTTGTGAGGAATGCGGAAAAACATTTATTTCCCATTCAAACCTTCAAAG
ACACAGGATAATGCACCGTGGAGATGGACCTTATAAGTGTAAATTTTGTGGGAAAGCCTT
GATGTTTCTCAGTTTGTATCTTATCCACAAACGAACTCACACTGGAGAGAAACCATATCA
ATGTAAACAGTGTGGTAAAGCCTTTAGTCATTCTAGTAGCCTTCGAATACATGAAAGAAC
TCACACTGGGGAGAAGCCTTATAAATGTAATGAATGTGGGAAAGCATTCCATAGTTCCAC
ATGCCTTCATGCTCATAAAAGAACTCACACTGGGGAGAAGCCATATGAATGTAAACAGTG
TGGGAAAGCCTTCAGCTCTTCCCATTCCTTTCAAATACATGAAAGAACTCACACGGGGGA
GAAGCCATATGAATGTAAGGAATGTGGAAAAGCATTCAAGTGTCCCAGTTCTGTTCGCAG
ACATGAAAGAACCCACTCTAGGAAAAAACCCTATGAATGTAAACATTGTGGGAAAGTATT
ATCTTATCTTACCAGCTTTCAAAACCACTTGGGAATGCACACTGGAGAGATATCTCATAA
ATGTAAGATATGTGGGAAAGCCTTTTATTCTCCCAGTTCACTTCAAACACATGAAAAAAC
TCACACTGGAGAGAAACCCTATAAATGCAACCAATGTGGTAAAGCCTTTAATTCTTCCAG
TTCCTTCCGATATCATGAAAGAACTCACACTGGAGAGAAACCTTACGAGTGTAAGCAATG
TGGGAAAGCCTTCAGATCTGCCTCACTCCTTCAAACACATGGTAGGACTCACACGGGAGA
GAAACCCTATGCATGTAAGGAATGTGGAAAACCATTTAGTAATTTCTCTTTCTTTCAAAT
ACATGAAAGGATGCACAGAGAAGAGAAGCCGTATGAATGTAAGGGTTATGGGAAAACATT
CAGTTTGCCCAGTTTATTTCATAGACATGAAAGGACTCACACTGGAGGAAAAACCTATGA
ATGCAAGCAGTGTGGCAGATCCTTCAACTGTTCGAGCTCCTTTCGATATCATGGAAGGAC
TCACACTGGAGAGAAACCCTATGAATGCAAGCAATGTGGAAAAGCCTTCAGATCTGCCTC
ACAGCTTCAAATTCATGGAAGGACTCACACTGGAGAGAAACCTTATGAATGTAAGCAGTG
TGGGAAAGCCTTTGGATCTGCCTCACACCTTCAAATGCATGGAAGGACTCACACTGGAGA
GAAACCCTATGAATGTAAGCAGTGTGGGAAGTCTTTTGGATGTGCCTCGCGACTTCAAAT
GCATGGAAGGACTCACACTGGAGAGAAACCGTATAAATGTAAGCAATGTGGGAAAGCTTT
TGGATGTCCCTCAAACCTTCGAAGGCATGGAAGGACTCACACTGGAGAGAAACCCTATAA
ATGTAACCAATGTGGTAAAGTCTTTAGATGTTCTTCACAACTTCAAGTGCATGGAAGGGC
TCACTGCATAGACACCCCAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT