UBA5 ORF clone pFN21AB8686

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Halotag ORF Clone pFN21AB8686

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Clone info.

Species

human

Clone Name

pFN21AB8686 Sequence (with vector) Restriction Map

Product ID

FHC02936

Flexi Clone PID

FXC02936

ORF Sequence

DNA sequence (1212 bp)
Protein sequence (404 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463333]

Gene

KIBB8686

Symbol

UBA5

Alias : DEE44, EIEE44, SCAR24, THIFP1, UBE1DC1

Description

ubiquitin-like modifier activating enzyme 5, transcript variant 1

Original Clone ID

oc00349

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1212 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 404 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3076.1 4.9e-165 100.0 UBA5 CCDS3077.1 4.3e-142 100.0 UBA5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000594 74 210 PF00899 UBA/THIF-type NAD/FAD binding fold
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_079094 404 610552,617132,617133 100.0 100.0 NP_001307139 348 610552,617132,617133 100.0 86.1 NP_938143 348 610552,617132,617133 100.0 86.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8686 Download

>KIBB8686  1212 bp
ATGGCGGAGTCTGTGGAGCGCCTGCAGCAGCGGGTCCAGGAGCTGGAGCGGGAACTTGCC
CAGGAGAGGAGTCTGCAGGTCCCGAGGAGCGGCGACGGAGGGGGCGGCCGGGTCCGCATC
GAGAAGATGAGCTCAGAGGTGGTGGATTCGAATCCCTACAGCCGCTTGATGGCATTGAAA
CGAATGGGAATTGTAAGCGACTATGAGAAAATCCGTACCTTTGCCGTAGCAATAGTAGGT
GTTGGTGGAGTAGGTAGTGTGACTGCTGAAATGCTGACAAGATGTGGCATTGGTAAGTTG
CTACTCTTTGATTATGACAAGGTGGAACTAGCCAATATGAATAGACTTTTCTTCCAACCT
CATCAAGCAGGATTAAGTAAAGTTCAAGCAGCAGAACATACTCTGAGGAACATTAATCCT
GATGTTCTTTTTGAAGTACACAACTATAATATAACCACAGTGGAAAACTTTCAACATTTC
ATGGATAGAATAAGTAATGGTGGGTTAGAAGAAGGAAAACCTGTTGATCTAGTTCTTAGC
TGTGTGGACAATTTTGAAGCTCGAATGACAATAAATACAGCTTGTAATGAACTTGGACAA
ACATGGATGGAATCTGGGGTCAGTGAAAATGCAGTTTCAGGGCATATACAGCTTATAATT
CCTGGAGAATCTGCTTGTTTTGCGTGTGCTCCACCACTTGTAGTTGCTGCAAATATTGAT
GAAAAGACTCTGAAACGAGAAGGTGTTTGTGCAGCCAGTCTTCCTACCACTATGGGTGTG
GTTGCTGGGATCTTAGTACAAAACGTGTTAAAGTTTCTGTTAAATTTTGGTACTGTTAGT
TTTTACCTTGGATACAATGCAATGCAGGATTTTTTTCCTACTATGTCCATGAAGCCAAAT
CCTCAGTGTGATGACAGAAATTGCAGGAAGCAGCAGGAGGAATATAAGAAAAAGGTAGCA
GCACTGCCTAAACAAGAGGTTATACAAGAAGAGGAAGAGATAATCCATGAAGATAATGAA
TGGGGTATTGAGCTGGTATCTGAGGTTTCAGAAGAGGAACTGAAAAATTTTTCAGGTCCA
GTTCCAGACTTACCTGAAGGAATTACAGTGGCATACACAATTCCAAAAAAGCAAGAAGAT
TCTGTCACTGAGTTAACAGTGGAAGATTCTGGTGAAAGCTTGGAAGACCTCATGGCCAAA
ATGAAGAATATG

ORF Protein

Cloned ORF protein sequence for pFN21AB8686 Download

>KIBB8686  404 aa
MAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALK
RMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQP
HQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLS
CVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANID
EKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPN
PQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGP
VPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8686 Download

>pFN21AB8686  5917 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGAGTCTGTGGAGCGCCTGCAGCAGCGGGT
CCAGGAGCTGGAGCGGGAACTTGCCCAGGAGAGGAGTCTGCAGGTCCCGAGGAGCGGCGA
CGGAGGGGGCGGCCGGGTCCGCATCGAGAAGATGAGCTCAGAGGTGGTGGATTCGAATCC
CTACAGCCGCTTGATGGCATTGAAACGAATGGGAATTGTAAGCGACTATGAGAAAATCCG
TACCTTTGCCGTAGCAATAGTAGGTGTTGGTGGAGTAGGTAGTGTGACTGCTGAAATGCT
GACAAGATGTGGCATTGGTAAGTTGCTACTCTTTGATTATGACAAGGTGGAACTAGCCAA
TATGAATAGACTTTTCTTCCAACCTCATCAAGCAGGATTAAGTAAAGTTCAAGCAGCAGA
ACATACTCTGAGGAACATTAATCCTGATGTTCTTTTTGAAGTACACAACTATAATATAAC
CACAGTGGAAAACTTTCAACATTTCATGGATAGAATAAGTAATGGTGGGTTAGAAGAAGG
AAAACCTGTTGATCTAGTTCTTAGCTGTGTGGACAATTTTGAAGCTCGAATGACAATAAA
TACAGCTTGTAATGAACTTGGACAAACATGGATGGAATCTGGGGTCAGTGAAAATGCAGT
TTCAGGGCATATACAGCTTATAATTCCTGGAGAATCTGCTTGTTTTGCGTGTGCTCCACC
ACTTGTAGTTGCTGCAAATATTGATGAAAAGACTCTGAAACGAGAAGGTGTTTGTGCAGC
CAGTCTTCCTACCACTATGGGTGTGGTTGCTGGGATCTTAGTACAAAACGTGTTAAAGTT
TCTGTTAAATTTTGGTACTGTTAGTTTTTACCTTGGATACAATGCAATGCAGGATTTTTT
TCCTACTATGTCCATGAAGCCAAATCCTCAGTGTGATGACAGAAATTGCAGGAAGCAGCA
GGAGGAATATAAGAAAAAGGTAGCAGCACTGCCTAAACAAGAGGTTATACAAGAAGAGGA
AGAGATAATCCATGAAGATAATGAATGGGGTATTGAGCTGGTATCTGAGGTTTCAGAAGA
GGAACTGAAAAATTTTTCAGGTCCAGTTCCAGACTTACCTGAAGGAATTACAGTGGCATA
CACAATTCCAAAAAAGCAAGAAGATTCTGTCACTGAGTTAACAGTGGAAGATTCTGGTGA
AAGCTTGGAAGACCTCATGGCCAAAATGAAGAATATGGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT