GPR4 ORF clone pFN21AB8811

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Halotag ORF Clone pFN21AB8811

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Clone info.

Species

human

Clone Name

pFN21AB8811 Sequence (with vector) Restriction Map

Product ID

FHC03039

Flexi Clone PID

FXC03039

ORF Sequence

DNA sequence (1086 bp)
Protein sequence (362 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464546]

Gene

KIBB8811

Symbol

GPR4

Alias : GPR6C.l

Description

G protein-coupled receptor 4

Original Clone ID

cp00846

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1086 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 362 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12669.1 3.1e-111 100.0 GPR4 CCDS9894.2 5e-47 49.1 GPR68 CCDS9879.1 2.7e-32 41.4 GPR65 CCDS61567.1 4.1e-26 38.8 GPR132 CCDS9997.1 4.1e-26 38.8 GPR132 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002276 1 22 PR01147 GPR4 orphan receptor IPR000276 19 43 PR00237 GPCR IPR000276 52 73 PR00237 GPCR IPR000276 97 119 PR00237 GPCR IPR002276 124 136 PR01147 GPR4 orphan receptor IPR002276 148 166 PR01147 GPR4 orphan receptor IPR002276 174 183 PR01147 GPR4 orphan receptor IPR000276 182 205 PR00237 GPCR IPR002276 208 220 PR01147 GPR4 orphan receptor IPR000276 222 246 PR00237 GPCR IPR002276 246 261 PR01147 GPR4 orphan receptor IPR000276 268 294 PR00237 GPCR IPR002276 300 318 PR01147 GPR4 orphan receptor IPR002276 319 337 PR01147 GPR4 orphan receptor HMMPfam IPR000276 35 286 PF00001 GPCR ProfileScan IPR017452 34 286 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 20 PPSLYIFVIGVGLPTNCLALWAA 42 SECONDARY 23 2 54 VYLMNLSIADLLYICTLPLWVDY 76 SECONDARY 23 3 95 FIFYTNIYISIAFLCCISVDRYL 117 SECONDARY 23 4 183 LYRVFVGFLFPWALMLLSYRGIL 205 PRIMARY 23 5 226 ALSLIAIVLVCFAPYHVLLLSRS 248 PRIMARY 23 6 271 SLAFTSLNCVADPILYCLVNEGA 293 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005273 362 600551 100.0 100.0 XP_016882097 362 600551 100.0 100.0 XP_016882096 362 600551 100.0 100.0 NP_003476 365 601404 49.1 91.7 NP_001171147 365 601404 49.1 91.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8811 Download

>KIBB8811  1086 bp
ATGGGCAACCACACGTGGGAGGGCTGCCACGTGGACTCGCGCGTGGACCACCTCTTTCCG
CCATCCCTCTACATCTTTGTCATCGGCGTGGGGCTGCCCACCAACTGCCTGGCTCTGTGG
GCGGCCTACCGCCAGGTGCAACAGCGCAACGAGCTGGGCGTCTACCTGATGAACCTCAGC
ATCGCCGACCTGCTGTACATCTGCACGCTGCCGCTGTGGGTGGACTACTTCCTGCACCAC
GACAACTGGATCCACGGCCCCGGGTCCTGCAAGCTCTTTGGGTTCATCTTCTACACCAAT
ATCTACATCAGCATCGCCTTCCTGTGCTGCATCTCGGTGGACCGCTACCTGGCTGTGGCC
CACCCACTCCGCTTCGCCCGCCTGCGCCGCGTCAAGACCGCCGTGGCCGTGAGCTCCGTG
GTCTGGGCCACGGAGCTGGGCGCCAACTCGGCGCCCCTGTTCCATGACGAGCTCTTCCGA
GACCGCTACAACCACACCTTCTGCTTTGAGAAGTTCCCCATGGAAGGTTGGGTGGCCTGG
ATGAACCTCTATCGGGTGTTCGTGGGCTTCCTCTTCCCGTGGGCGCTCATGCTGCTGTCG
TACCGGGGCATCCTGCGGGCCGTGCGGGGCAGCGTGTCCACCGAGCGCCAGGAGAAGGCC
AAGATCAAGCGGCTGGCCCTCAGCCTCATCGCCATCGTGCTGGTCTGCTTTGCGCCCTAT
CACGTGCTCTTGCTGTCCCGCAGCGCCATCTACCTGGGCCGCCCCTGGGACTGCGGCTTC
GAGGAGCGCGTCTTTTCTGCATACCACAGCTCACTGGCTTTCACCAGCCTCAACTGTGTG
GCGGACCCCATCCTCTACTGCCTGGTCAACGAGGGCGCCCGCAGCGATGTGGCCAAGGCC
CTGCACAACCTGCTCCGCTTTCTGGCCAGCGACAAGCCCCAGGAGATGGCCAATGCCTCG
CTCACCCTGGAGACCCCACTCACCTCCAAGAGGAACAGCACAGCCAAAGCCATGACTGGC
AGCTGGGCGGCCACTCCGCCCTCCCAGGGGGACCAGGTGCAGCTGAAGATGCTGCCGCCA
GCACAA

ORF Protein

Cloned ORF protein sequence for pFN21AB8811 Download

>KIBB8811  362 aa
MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLS
IADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVA
HPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAW
MNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPY
HVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKA
LHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPP
AQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8811 Download

>pFN21AB8811  5791 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCAACCACACGTGGGAGGGCTGCCACGTGGA
CTCGCGCGTGGACCACCTCTTTCCGCCATCCCTCTACATCTTTGTCATCGGCGTGGGGCT
GCCCACCAACTGCCTGGCTCTGTGGGCGGCCTACCGCCAGGTGCAACAGCGCAACGAGCT
GGGCGTCTACCTGATGAACCTCAGCATCGCCGACCTGCTGTACATCTGCACGCTGCCGCT
GTGGGTGGACTACTTCCTGCACCACGACAACTGGATCCACGGCCCCGGGTCCTGCAAGCT
CTTTGGGTTCATCTTCTACACCAATATCTACATCAGCATCGCCTTCCTGTGCTGCATCTC
GGTGGACCGCTACCTGGCTGTGGCCCACCCACTCCGCTTCGCCCGCCTGCGCCGCGTCAA
GACCGCCGTGGCCGTGAGCTCCGTGGTCTGGGCCACGGAGCTGGGCGCCAACTCGGCGCC
CCTGTTCCATGACGAGCTCTTCCGAGACCGCTACAACCACACCTTCTGCTTTGAGAAGTT
CCCCATGGAAGGTTGGGTGGCCTGGATGAACCTCTATCGGGTGTTCGTGGGCTTCCTCTT
CCCGTGGGCGCTCATGCTGCTGTCGTACCGGGGCATCCTGCGGGCCGTGCGGGGCAGCGT
GTCCACCGAGCGCCAGGAGAAGGCCAAGATCAAGCGGCTGGCCCTCAGCCTCATCGCCAT
CGTGCTGGTCTGCTTTGCGCCCTATCACGTGCTCTTGCTGTCCCGCAGCGCCATCTACCT
GGGCCGCCCCTGGGACTGCGGCTTCGAGGAGCGCGTCTTTTCTGCATACCACAGCTCACT
GGCTTTCACCAGCCTCAACTGTGTGGCGGACCCCATCCTCTACTGCCTGGTCAACGAGGG
CGCCCGCAGCGATGTGGCCAAGGCCCTGCACAACCTGCTCCGCTTTCTGGCCAGCGACAA
GCCCCAGGAGATGGCCAATGCCTCGCTCACCCTGGAGACCCCACTCACCTCCAAGAGGAA
CAGCACAGCCAAAGCCATGACTGGCAGCTGGGCGGCCACTCCGCCCTCCCAGGGGGACCA
GGTGCAGCTGAAGATGCTGCCGCCAGCACAAGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT