GTF2F1 ORF clone pFN21AB9482

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB9482

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Clone info.

Species

human

Clone Name

pFN21AB9482 Sequence (with vector) Restriction Map

Product ID

FHC01901

Flexi Clone PID

FXC01901

Original Clone PID

ORK01901

ORF Sequence

DNA sequence (1551 bp)
Protein sequence (517 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385238]

Gene

KIBB9482

Symbol

GTF2F1

Alias : BTF4, RAP74, TF2F1, TFIIF

Description

general transcription factor IIF, polypeptide 1, 74kDa

Original Clone ID

ef02191

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1551 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 517 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12165.1 1.1e-91 100.0 GTF2F1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR008851 2 517 PF05793 Transcription initiation factor IIF
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002087 517 189968 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9482 Download

>KIBB9482  1551 bp
ATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTCACTGAATACGTCGTTCGAGTTCCTAAG
AATACAACCAAAAAATATAACATCATGGCTTTTAATGCAGCCGACAAAGTCAACTTTGCT
ACGTGGAATCAGGCTCGGCTGGAGCGGGACTTGAGCAACAAGAAAATCTACCAAGAGGAG
GAGATGCCCGAATCGGGCGCGGGCAGTGAGTTCAACCGCAAGCTTCGGGAGGAGGCTCGG
AGGAAGAAGTACGGCATCGTCCTCAAGGAGTTCCGGCCCGAGGACCAGCCCTGGCTGCTC
CGGGTCAACGGCAAATCAGGCAGGAAGTTCAAGGGCATCAAGAAGGGAGGCGTAACAGAG
AACACGTCCTACTACATCTTCACCCAGTGCCCCGACGGGGCCTTCGAGGCCTTCCCCGTG
CACAACTGGTACAATTTCACACCGCTGGCCCGGCATCGCACGCTCACTGCCGAGGAGGCC
GAGGAGGAGTGGGAGAGGAGGAACAAGGTGCTGAACCACTTCAGCATCATGCAGCAGCGG
CGGCTCAAGGATCAGGACCAGGACGAGGATGAGGAGGAGAAGGAGAAACGTGGCCGCAGG
AAGGCGAGCGAGCTGCGCATCCACGACCTGGAGGACGACCTGGAGATGTCGTCCGATGCC
AGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTCCCCAAGGCCAAGAAGAAGGCGCCGCTG
GCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAGGGTTCAGACGACGAGGCCTTCGAGGAC
AGCGATGATGGGGACTTCGAGGGCCAAGAGGTGGACTACATGTCAGACGGCTCCAGTAGC
TCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCGCCGCAGCAGGAGGAGGGGCCCAAGGGT
GTCGATGAGCAGAGCGACAGTAGTGAGGAGAGTGAGGAGGAGAAGCCGCCTGAGGAGGAC
AAGGAGGAGGAGGAGGAGAAGAAGGCACCCACCCCGCAGGAGAAGAAGCGCAGGAAAGAC
AGCAGCGAGGAGTCGGACAGCTCAGAGGAGAGCGACATTGACAGCGAGGCCTCCTCAGCC
CTCTTCATGGCGAAGAAGAAGACGCCACCCAAGAGAGAGCGGAAGCCGTCGGGAGGGAGC
TCAAGGGGCAACAGCCGCCCAGGCACGCCCAGCGCAGAGGGTGGCAGCACCTCCTCCACC
CTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGGAAGCGGGTGAGCGAGATGCCTGCAGCC
AAGCGGTTGCGGCTGGACACGGGACCCCAGAGCCTGTCTGGGAAGTCGACACCCCAGCCA
CCATCAGGCAAGACAACACCCAACAGCGGCGACGTGCAGGTGACTGAGGATGCCGTGCGC
CGCTACCTGACACGGAAGCCCATGACCACTAAGGACCTGCTGAAAAAGTTCCAGACCAAG
AAGACAGGGCTGAGCAGCGAGCAGACAGTGAACGTGTTGGCCCAGATCCTCAAGCGACTC
AACCCCGAGCGCAAGATGATCAACGACAAAATGCACTTCTCCCTCAAGGAG

ORF Protein

Cloned ORF protein sequence for pFN21AB9482 Download

>KIBB9482  517 aa
MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEE
EMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTE
NTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQR
RLKDQDQDEDEEEKEKRGRRKASELRIHDLEDDLEMSSDASDASGEEGGRVPKAKKKAPL
AKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSSSQEEPESKAKAPQQEEGPKG
VDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEESDIDSEASSA
LFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAA
KRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTK
KTGLSSEQTVNVLAQILKRLNPERKMINDKMHFSLKE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9482 Download

>pFN21AB9482  6256 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTCAC
TGAATACGTCGTTCGAGTTCCTAAGAATACAACCAAAAAATATAACATCATGGCTTTTAA
TGCAGCCGACAAAGTCAACTTTGCTACGTGGAATCAGGCTCGGCTGGAGCGGGACTTGAG
CAACAAGAAAATCTACCAAGAGGAGGAGATGCCCGAATCGGGCGCGGGCAGTGAGTTCAA
CCGCAAGCTTCGGGAGGAGGCTCGGAGGAAGAAGTACGGCATCGTCCTCAAGGAGTTCCG
GCCCGAGGACCAGCCCTGGCTGCTCCGGGTCAACGGCAAATCAGGCAGGAAGTTCAAGGG
CATCAAGAAGGGAGGCGTAACAGAGAACACGTCCTACTACATCTTCACCCAGTGCCCCGA
CGGGGCCTTCGAGGCCTTCCCCGTGCACAACTGGTACAATTTCACACCGCTGGCCCGGCA
TCGCACGCTCACTGCCGAGGAGGCCGAGGAGGAGTGGGAGAGGAGGAACAAGGTGCTGAA
CCACTTCAGCATCATGCAGCAGCGGCGGCTCAAGGATCAGGACCAGGACGAGGATGAGGA
GGAGAAGGAGAAACGTGGCCGCAGGAAGGCGAGCGAGCTGCGCATCCACGACCTGGAGGA
CGACCTGGAGATGTCGTCCGATGCCAGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTCCC
CAAGGCCAAGAAGAAGGCGCCGCTGGCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAGGG
TTCAGACGACGAGGCCTTCGAGGACAGCGATGATGGGGACTTCGAGGGCCAAGAGGTGGA
CTACATGTCAGACGGCTCCAGTAGCTCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCGCC
GCAGCAGGAGGAGGGGCCCAAGGGTGTCGATGAGCAGAGCGACAGTAGTGAGGAGAGTGA
GGAGGAGAAGCCGCCTGAGGAGGACAAGGAGGAGGAGGAGGAGAAGAAGGCACCCACCCC
GCAGGAGAAGAAGCGCAGGAAAGACAGCAGCGAGGAGTCGGACAGCTCAGAGGAGAGCGA
CATTGACAGCGAGGCCTCCTCAGCCCTCTTCATGGCGAAGAAGAAGACGCCACCCAAGAG
AGAGCGGAAGCCGTCGGGAGGGAGCTCAAGGGGCAACAGCCGCCCAGGCACGCCCAGCGC
AGAGGGTGGCAGCACCTCCTCCACCCTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGGAA
GCGGGTGAGCGAGATGCCTGCAGCCAAGCGGTTGCGGCTGGACACGGGACCCCAGAGCCT
GTCTGGGAAGTCGACACCCCAGCCACCATCAGGCAAGACAACACCCAACAGCGGCGACGT
GCAGGTGACTGAGGATGCCGTGCGCCGCTACCTGACACGGAAGCCCATGACCACTAAGGA
CCTGCTGAAAAAGTTCCAGACCAAGAAGACAGGGCTGAGCAGCGAGCAGACAGTGAACGT
GTTGGCCCAGATCCTCAAGCGACTCAACCCCGAGCGCAAGATGATCAACGACAAAATGCA
CTTCTCCCTCAAGGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT