KRT32 ORF clone pFN21AE0564

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0564

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Clone info.

Species

human

Clone Name

pFN21AE0564 Sequence (with vector) Restriction Map

Product ID

FHC11164

Flexi Clone PID

FXC11164

ORF Sequence

DNA sequence (1344 bp)
Protein sequence (448 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528846]

Gene

KIEE0564

Symbol

KRT32

Alias : HA2, HKA2, KRTHA2, hHa2

Description

keratin 32, type I

Original Clone ID

oc01264

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1344 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 448 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11393.1 3e-108 100.0 KRT32 CCDS11394.2 1.7e-66 67.0 KRT35 CCDS11395.1 5.7e-66 66.4 KRT36 CCDS11391.1 1.6e-63 69.1 KRT31 CCDS11388.1 4.6e-61 68.1 KRT33A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002957 174 187 PR01248 Keratin IPR002957 195 218 PR01248 Keratin IPR002957 249 269 PR01248 Keratin IPR002957 321 336 PR01248 Keratin IPR002957 347 373 PR01248 Keratin HMMPfam IPR016044 95 406 PF00038 Filament
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002269 448 602760 100.0 100.0 XP_011523096 455 602764 67.0 100.0 NP_002271 455 602764 67.0 100.0 NP_003762 467 604540 66.4 99.3 NP_002268 416 601077 69.1 89.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0564 Download

>KIEE0564  1344 bp
ATGACATCCTCCTGCTGTGTCACCAACAACTTGCAAGCCTCTCTCAAGAGCTGCCCCCGG
CCTGCCTCGGTCTGTTCCAGCGGCGTGAACTGCCGGCCTGAGCTGTGCCTGGGCTATGTC
TGCCAGCCCATGGCATGCCTGCCTTCGGTCTGCCTGCCCACCACCTTCCGGCCAGCCAGC
TGCCTCTCCAAAACCTATCTATCCAGTTCCTGCCAGGCAGCCAGTGGCATCTCCGGCTCC
ATGGGCCCCGGCAGCTGGTACAGCGAAGGGGCCTTCAATGGCAATGAGAAGGAAACCATG
CAGTTCCTTAACGACCGCCTGGCCAGCTACCTGACGAGGGTGCGGCAGCTGGAGCAGGAG
AATGCGGAGCTGGAGAGCAGGATCCAAGAGGCCTCTCACTCCCAGGTGCTCACCATGACT
CCTGACTACCAGTCTCATTTCAGGACCATTGAGGAGCTCCAGCAGAAGATTCTGTGTACC
AAGGCAGAGAATGCCAGGATGGTTGTGAACATTGATAATGCCAAACTGGCTGCCGATGAC
TTCAGGGCCAAGTACGAGGCAGAGCTGGCCATGCGGCAGCTGGTGGAGGCCGACATCAAT
GGCCTGCGCAGGATCCTGGATGATCTCACTCTGTGCAAGGCTGACCTGGAGGCCCAGGTT
GAGTCCCTGAAGGAGGAGCTGATGTGCCTCAAAAAGAACCATGAGGAGGAAGTCGGTTCC
CTTCGATGCCAGCTTGGGGACCGCCTTAACATCGAGGTGGACGCTGCACCCCCGGTGGAC
CTGACCAGGGTGCTGGAGGAGATGCGGTGTCAGTACGAGGCCATGGTGGAGGCCAACCGC
AGGGACGTGGAGGAATGGTTCAATATGCAGATGGAGGAGCTTAACCAACAGGTGGCCACA
AGCTCTGAGCAGCTTCAGAACTACCAGTCAGACATCATTGACCTGAGACGCACGGTCAAC
ACGCTGGAGATCGAGCTGCAGGCCCAGCACAGCCTGAGGGACTCCCTGGAAAACACGCTG
ACGGAGAGTGAGGCCCGCTACAGCTCCCAGCTGGCCCAGATGCAGTGCATGATCACCAAC
GTTGAGGCCCAGCTGGCTGAGATCCGGGCTGACCTGGAGCGGCAGAACCAGGAGTACCAG
GTGCTGCTGGACGTCCGGGCCCGGCTGGAGGGCGAGATCAACACGTACCGGAGCCTGCTG
GAGAACGAGGACTGCAAGCTGCCCTGTAACCCATGCTCCACTCCTTCCTGCACCACCTGT
GTGCCCTCCCCATGCGTGCCCCGCACCGTCTGTGTGCCACGCACTGTTGGCATGCCTTGC
TCACCCTGCCCCCAGGGCCGCTAC

ORF Protein

Cloned ORF protein sequence for pFN21AE0564 Download

>KIEE0564  448 aa
MTSSCCVTNNLQASLKSCPRPASVCSSGVNCRPELCLGYVCQPMACLPSVCLPTTFRPAS
CLSKTYLSSSCQAASGISGSMGPGSWYSEGAFNGNEKETMQFLNDRLASYLTRVRQLEQE
NAELESRIQEASHSQVLTMTPDYQSHFRTIEELQQKILCTKAENARMVVNIDNAKLAADD
FRAKYEAELAMRQLVEADINGLRRILDDLTLCKADLEAQVESLKEELMCLKKNHEEEVGS
LRCQLGDRLNIEVDAAPPVDLTRVLEEMRCQYEAMVEANRRDVEEWFNMQMEELNQQVAT
SSEQLQNYQSDIIDLRRTVNTLEIELQAQHSLRDSLENTLTESEARYSSQLAQMQCMITN
VEAQLAEIRADLERQNQEYQVLLDVRARLEGEINTYRSLLENEDCKLPCNPCSTPSCTTC
VPSPCVPRTVCVPRTVGMPCSPCPQGRY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0564 Download

>pFN21AE0564  6049 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACATCCTCCTGCTGTGTCACCAACAACTTGCA
AGCCTCTCTCAAGAGCTGCCCCCGGCCTGCCTCGGTCTGTTCCAGCGGCGTGAACTGCCG
GCCTGAGCTGTGCCTGGGCTATGTCTGCCAGCCCATGGCATGCCTGCCTTCGGTCTGCCT
GCCCACCACCTTCCGGCCAGCCAGCTGCCTCTCCAAAACCTATCTATCCAGTTCCTGCCA
GGCAGCCAGTGGCATCTCCGGCTCCATGGGCCCCGGCAGCTGGTACAGCGAAGGGGCCTT
CAATGGCAATGAGAAGGAAACCATGCAGTTCCTTAACGACCGCCTGGCCAGCTACCTGAC
GAGGGTGCGGCAGCTGGAGCAGGAGAATGCGGAGCTGGAGAGCAGGATCCAAGAGGCCTC
TCACTCCCAGGTGCTCACCATGACTCCTGACTACCAGTCTCATTTCAGGACCATTGAGGA
GCTCCAGCAGAAGATTCTGTGTACCAAGGCAGAGAATGCCAGGATGGTTGTGAACATTGA
TAATGCCAAACTGGCTGCCGATGACTTCAGGGCCAAGTACGAGGCAGAGCTGGCCATGCG
GCAGCTGGTGGAGGCCGACATCAATGGCCTGCGCAGGATCCTGGATGATCTCACTCTGTG
CAAGGCTGACCTGGAGGCCCAGGTTGAGTCCCTGAAGGAGGAGCTGATGTGCCTCAAAAA
GAACCATGAGGAGGAAGTCGGTTCCCTTCGATGCCAGCTTGGGGACCGCCTTAACATCGA
GGTGGACGCTGCACCCCCGGTGGACCTGACCAGGGTGCTGGAGGAGATGCGGTGTCAGTA
CGAGGCCATGGTGGAGGCCAACCGCAGGGACGTGGAGGAATGGTTCAATATGCAGATGGA
GGAGCTTAACCAACAGGTGGCCACAAGCTCTGAGCAGCTTCAGAACTACCAGTCAGACAT
CATTGACCTGAGACGCACGGTCAACACGCTGGAGATCGAGCTGCAGGCCCAGCACAGCCT
GAGGGACTCCCTGGAAAACACGCTGACGGAGAGTGAGGCCCGCTACAGCTCCCAGCTGGC
CCAGATGCAGTGCATGATCACCAACGTTGAGGCCCAGCTGGCTGAGATCCGGGCTGACCT
GGAGCGGCAGAACCAGGAGTACCAGGTGCTGCTGGACGTCCGGGCCCGGCTGGAGGGCGA
GATCAACACGTACCGGAGCCTGCTGGAGAACGAGGACTGCAAGCTGCCCTGTAACCCATG
CTCCACTCCTTCCTGCACCACCTGTGTGCCCTCCCCATGCGTGCCCCGCACCGTCTGTGT
GCCACGCACTGTTGGCATGCCTTGCTCACCCTGCCCCCAGGGCCGCTACGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT