SYT10 ORF clone pFN21AE0999

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0999

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Clone info.

Species

human

Clone Name

pFN21AE0999 Sequence (with vector) Restriction Map

Product ID

FHC12555

ORF Sequence

DNA sequence (1569 bp)
Protein sequence (523 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE0999

Symbol

SYT10

Description

synaptotagmin X

Original Clone ID

cp00703

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1569 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 523 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8732.1 2.3e-203 100.0 SYT10 CCDS871.1 3.2e-114 70.5 SYT6 CCDS7778.1 5.2e-103 59.2 SYT9 CCDS12798.1 3.4e-71 47.6 SYT3 CCDS76582.1 7.5e-51 49.3 SYT1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001565 235 250 PR00399 Synaptotagmin IPR001565 250 263 PR00399 Synaptotagmin IPR020477 263 275 PR00360 C2 region IPR020477 290 303 PR00360 C2 region IPR001565 305 320 PR00399 Synaptotagmin IPR020477 314 322 PR00360 C2 region IPR001565 325 335 PR00399 Synaptotagmin HMMPfam IPR000008 248 333 PF00168 C2 calcium-dependent membrane targeting IPR000008 381 467 PF00168 C2 calcium-dependent membrane targeting HMMSmart IPR000008 247 350 SM00239 C2 calcium-dependent membrane targeting IPR000008 379 493 SM00239 C2 calcium-dependent membrane targeting ProfileScan IPR018029 248 334 PS50004 C2 membrane targeting protein IPR018029 377 468 PS50004 C2 membrane targeting protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 54 VSLLAVVVSFCGLALLVVSLFVF 76 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016855860 443 607718 69.8 84.1 XP_011518203 564 613528 57.9 96.9 XP_011518202 567 613528 57.9 96.9 XP_011518206 492 613528 59.0 94.5 NP_783860 491 613528 59.2 93.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0999 Download

>KIEE0999  1569 bp
ATGAGTTTCCACAAGGAGGACGGAGTGAACAGTCTGTGCCAGAAGGCTCTGCACATCGTC
ACCGAGCTGTGCTTCGCCGGCCAGGTGGAGTGGGAGAAGTGCTCGGGCATCTTCCCTCGG
GACAGGGGCAGCCAGGGCGGAAGCAGCACAGATATTTCAGTCAGCCTGTTAGCTGTCGTT
GTCAGCTTTTGTGGACTGGCCTTGTTGGTTGTCTCACTTTTTGTCTTCTGGAAGCTGTGT
TGGCCATGCTGGAAAAGCAAACCTGTGACTTCCAACATCACTACGCTTCCACAGAGCATT
TCAAGTGCTCCTACTGAAGTTTTTGAGACTGAAGAGAAAAAAGAAATTAAGGAAAATGAA
AAGCCAGCCGTAAAAGCTATTGAGCCTGCAATAAAAATCAGCCACACTTCCCCTGACATC
CCAGCAGAAGTCCAAACTGCTTTAAAAGAACATTTAATTAAACATGCACGTGTGCAAAGA
CAAATTACTGAGCCTACGTCATCAACCCGCCACAGTTCCTTCCGAAGACACCTGCCGAGG
CAAATGCAGGTTTCCAGTGTTGATTTTAGCATGGGCACAGAACCTGTTTTACAACGAGGA
GAAACAACAACCAGCATTGGGAGGATAAAGCCAGAACTCTACAAACAGAAATCAGTTGAC
TCTGAGGGCAACCAAAACGAAGATGTCAAAATCTGTGGGAAACTTAACTTTACCCTCCAG
TATGATTATGAAAATGAACTTCTAGTTGTTAAAATTATCAAAGCTTTAGATCTCCCTGCT
AAAGACTTCACAGGAACTTCTGACCCTTATGTGAAGATGTATCTTCTTCCAGATAGGAAA
AAGAAATTTCAGACCCGCGTGCACAGAAAGACTTTAAATCCTCTATTTGATGAAACTTTT
CAATTTCCTGTAGCATATGATCAACTAAGCAACCGAAAACTACATTTCAGTGTGTATGAT
TTTGACAGATTTTCTAGACATGACATGATTGGGGAAGTGATTCTTGATAATTTGTTTGAA
GTCTCTGATCTCTCCAGGGAAGCCACAGTATGGAAAGATATTCACTGTGCTACCACAGAA
AGTATAGACCTGGGTGAAATCATGTTTTCCCTTTGTTACCTACCGACGGCTGGGCGTATG
ACATTGACAGTCATTAAGTGCAGAAATCTGAAGGCGATGGATATTACTGGCTCATCAGAT
CCTTATGTCAAAGTGTCCCTGATGTGTGAAGGTCGAAGATTAAAAAAGAGGAAAACAACT
ACAAAGAAAAACACTCTAAACCCTGTGTACAATGAGGCCATTATTTTTGACATCCCTCCA
GAGAACGTGGACCAGGTCAGCCTCTCCATTGCGGTCATGGATTACGATAGGGTAGGACAC
AATGAGGTCATAGGAGTGTGCAGAACAGGACTGGATGCTGAGGGTCTTGGGCGAGACCAC
TGGAATGAAATGCTGGCCTATCATCGAAAACCAATAACGCACTGGCACCCATTGCTGGAG
TTACCTGGCCGGGCGACCAGTTTTGATAGTCAAGGATCCTGCCCTTCTCCTAAACCACCT
TCCACACCA

ORF Protein

Cloned ORF protein sequence for pFN21AE0999 Download

>KIEE0999  523 aa
MSFHKEDGVNSLCQKALHIVTELCFAGQVEWEKCSGIFPRDRGSQGGSSTDISVSLLAVV
VSFCGLALLVVSLFVFWKLCWPCWKSKPVTSNITTLPQSISSAPTEVFETEEKKEIKENE
KPAVKAIEPAIKISHTSPDIPAEVQTALKEHLIKHARVQRQITEPTSSTRHSSFRRHLPR
QMQVSSVDFSMGTEPVLQRGETTTSIGRIKPELYKQKSVDSEGNQNEDVKICGKLNFTLQ
YDYENELLVVKIIKALDLPAKDFTGTSDPYVKMYLLPDRKKKFQTRVHRKTLNPLFDETF
QFPVAYDQLSNRKLHFSVYDFDRFSRHDMIGEVILDNLFEVSDLSREATVWKDIHCATTE
SIDLGEIMFSLCYLPTAGRMTLTVIKCRNLKAMDITGSSDPYVKVSLMCEGRRLKKRKTT
TKKNTLNPVYNEAIIFDIPPENVDQVSLSIAVMDYDRVGHNEVIGVCRTGLDAEGLGRDH
WNEMLAYHRKPITHWHPLLELPGRATSFDSQGSCPSPKPPSTP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0999 Download

>pFN21AE0999  6274 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTTTCCACAAGGAGGACGGAGTGAACAGTCT
GTGCCAGAAGGCTCTGCACATCGTCACCGAGCTGTGCTTCGCCGGCCAGGTGGAGTGGGA
GAAGTGCTCGGGCATCTTCCCTCGGGACAGGGGCAGCCAGGGCGGAAGCAGCACAGATAT
TTCAGTCAGCCTGTTAGCTGTCGTTGTCAGCTTTTGTGGACTGGCCTTGTTGGTTGTCTC
ACTTTTTGTCTTCTGGAAGCTGTGTTGGCCATGCTGGAAAAGCAAACCTGTGACTTCCAA
CATCACTACGCTTCCACAGAGCATTTCAAGTGCTCCTACTGAAGTTTTTGAGACTGAAGA
GAAAAAAGAAATTAAGGAAAATGAAAAGCCAGCCGTAAAAGCTATTGAGCCTGCAATAAA
AATCAGCCACACTTCCCCTGACATCCCAGCAGAAGTCCAAACTGCTTTAAAAGAACATTT
AATTAAACATGCACGTGTGCAAAGACAAATTACTGAGCCTACGTCATCAACCCGCCACAG
TTCCTTCCGAAGACACCTGCCGAGGCAAATGCAGGTTTCCAGTGTTGATTTTAGCATGGG
CACAGAACCTGTTTTACAACGAGGAGAAACAACAACCAGCATTGGGAGGATAAAGCCAGA
ACTCTACAAACAGAAATCAGTTGACTCTGAGGGCAACCAAAACGAAGATGTCAAAATCTG
TGGGAAACTTAACTTTACCCTCCAGTATGATTATGAAAATGAACTTCTAGTTGTTAAAAT
TATCAAAGCTTTAGATCTCCCTGCTAAAGACTTCACAGGAACTTCTGACCCTTATGTGAA
GATGTATCTTCTTCCAGATAGGAAAAAGAAATTTCAGACCCGCGTGCACAGAAAGACTTT
AAATCCTCTATTTGATGAAACTTTTCAATTTCCTGTAGCATATGATCAACTAAGCAACCG
AAAACTACATTTCAGTGTGTATGATTTTGACAGATTTTCTAGACATGACATGATTGGGGA
AGTGATTCTTGATAATTTGTTTGAAGTCTCTGATCTCTCCAGGGAAGCCACAGTATGGAA
AGATATTCACTGTGCTACCACAGAAAGTATAGACCTGGGTGAAATCATGTTTTCCCTTTG
TTACCTACCGACGGCTGGGCGTATGACATTGACAGTCATTAAGTGCAGAAATCTGAAGGC
GATGGATATTACTGGCTCATCAGATCCTTATGTCAAAGTGTCCCTGATGTGTGAAGGTCG
AAGATTAAAAAAGAGGAAAACAACTACAAAGAAAAACACTCTAAACCCTGTGTACAATGA
GGCCATTATTTTTGACATCCCTCCAGAGAACGTGGACCAGGTCAGCCTCTCCATTGCGGT
CATGGATTACGATAGGGTAGGACACAATGAGGTCATAGGAGTGTGCAGAACAGGACTGGA
TGCTGAGGGTCTTGGGCGAGACCACTGGAATGAAATGCTGGCCTATCATCGAAAACCAAT
AACGCACTGGCACCCATTGCTGGAGTTACCTGGCCGGGCGACCAGTTTTGATAGTCAAGG
ATCCTGCCCTTCTCCTAAACCACCTTCCACACCAGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT