NSUN5 ORF clone pFN21AE1443

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1443

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Clone info.

Species

human

Clone Name

pFN21AE1443 Sequence (with vector) Restriction Map

Product ID

FHC08363

ORF Sequence

DNA sequence (1398 bp)
Protein sequence (466 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590182]

Gene

KIEE1443

Symbol

NSUN5

Alias : NOL1, NOL1R, NSUN5A, WBSCR20, WBSCR20A, p120, p120(NOL1)

Description

NOP2/Sun domain family, member 5, transcript variant 1

Original Clone ID

oc01443

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1398 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 466 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5546.1 2.2e-181 100.0 NSUN5 CCDS55119.1 2.2e-168 95.4 NSUN5 CCDS5547.1 6.8e-166 100.0 NSUN5 CCDS55118.1 2.1e-125 91.1 NSUN5 CCDS44811.1 2.2e-16 32.2 NOP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR023267 202 216 PR02008 RNA (C5-cytosine) methyltransferase IPR023267 231 241 PR02008 RNA (C5-cytosine) methyltransferase IPR023267 300 312 PR02008 RNA (C5-cytosine) methyltransferase IPR023267 351 367 PR02008 RNA (C5-cytosine) methyltransferase IPR023267 407 424 PR02008 RNA (C5-cytosine) methyltransferase HMMPfam IPR001678 191 421 PF01189 Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_683759 466 615732 100.0 100.0 NP_001161819 470 615732 95.4 97.9 NP_060514 429 615732 100.0 91.8 NP_001161820 391 615732 91.1 91.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1443 Download

>KIEE1443  1398 bp
ATGGGGCTGTATGCTGCAGCTGCAGGCGTGTTGGCCGGCGTGGAGAGCCGCCAGGGCTCT
ATCAAGGGGTTGGTGTACTCCAGCAACTTCCAGAACGTGAAGCAGCTGTACGCGCTGGTG
TGCGAAACGCAGCGCTACTCCGCCGTGCTGGATGCTGTGATCGCCAGCGCCGGCCTCCTC
CGTGCGGAGAAGAAGCTGCGGCCGCACCTGGCCAAGGTGCTAGTGTATGAGTTGTTGTTG
GGAAAGGGCTTTCGAGGGGGTGGGGGCCGATGGAAGGCTCTGTTGGGCCGGCACCAGGCG
AGGCTCAAGGCTGAGTTGGCTCGGCTCAAGGTTCATCGGGGTGTGAGCCGGAATGAGGAC
CTGTTGGAAGTGGGATCCAGGCCTGGTCCAGCCTCCCAGCTGCCTCGATTTGTGCGTGTG
AACACTCTCAAGACCTGCTCCGATGATGTAGTTGATTATTTCAAGAGACAAGGTTTCTCC
TATCAGGGTCGGGCTTCCAGCCTCGATGACTTACGAGCCCTCAAGGGGAAGCATTTTCTC
CTGGACCCCTTGATGCCGGAGCTGCTGGTGTTTCCCGCCCAGACAGATCTGCATGAACAC
CCACTGTACCGGGCCGGACACCTCATTCTGCAGGACAGGGCCAGCTGTCTCCCAGCCATG
CTGCTGGACCCCCCGCCAGGCTCCCATGTCATCGATGCCTGTGCCGCCCCAGGCAATAAG
ACCAGTCACTTGGCTGCTCTTCTGAAGAACCAAGGGAAGATCTTTGCCTTTGACCTGGAT
GCCAAGCGGCTGGCATCCATGGCCACGCTGCTGGCCCGGGCTGGCGTCTCTTGCTGTGAA
CTGGCTGAGGAGGACTTCCTGGCGGTCTCCCCCTCGGATCCACGCTACCATGAGGTCCAC
TACATCCTGCTGGATCCTTCCTGCAGTGGCTCGGGTATGCCGAGCAGACAGCTGGAGGAG
CCCGGGGCAGGCACACCTAGCCCGGTGCGTCTGCATGCCCTGGCAGGGTTCCAGCAGCGA
GCCCTGTGCCACGCACTCACTTTCCCTTCCCTGCAGCGGCTCGTCTACTCCACGTGCTCC
CTCTGCCAGGAGGAGAATGAAGACGTGGTGCGAGATGCGCTGCAGCAGAACCCGGGCGCC
TTCAGGCTAGCTCCCGCCCTGCCTGCCTGGCCCCACCGAGGCCTGAGCACGTTCCCGGGT
GCCGAGCACTGCCTCCGGGCCTCCCCTGAGACCACACTCAGCAGTGGCTTCTTCGTTGCT
GTAATTGAACGGGTCGAGGTGCCAAGCTCAGCCTCACAGGCCAAAGCATCAGCACCAGAA
CGCACACCCAGCCCAGCCCCAAAGAGAAAGAAGAGACAGCAAAGAGCCGCAGCCGGTGCT
TGCACACCGCCTTGCACA

ORF Protein

Cloned ORF protein sequence for pFN21AE1443 Download

>KIEE1443  466 aa
MGLYAAAAGVLAGVESRQGSIKGLVYSSNFQNVKQLYALVCETQRYSAVLDAVIASAGLL
RAEKKLRPHLAKVLVYELLLGKGFRGGGGRWKALLGRHQARLKAELARLKVHRGVSRNED
LLEVGSRPGPASQLPRFVRVNTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFL
LDPLMPELLVFPAQTDLHEHPLYRAGHLILQDRASCLPAMLLDPPPGSHVIDACAAPGNK
TSHLAALLKNQGKIFAFDLDAKRLASMATLLARAGVSCCELAEEDFLAVSPSDPRYHEVH
YILLDPSCSGSGMPSRQLEEPGAGTPSPVRLHALAGFQQRALCHALTFPSLQRLVYSTCS
LCQEENEDVVRDALQQNPGAFRLAPALPAWPHRGLSTFPGAEHCLRASPETTLSSGFFVA
VIERVEVPSSASQAKASAPERTPSPAPKRKKRQQRAAAGACTPPCT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1443 Download

>pFN21AE1443  6103 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGCTGTATGCTGCAGCTGCAGGCGTGTTGGC
CGGCGTGGAGAGCCGCCAGGGCTCTATCAAGGGGTTGGTGTACTCCAGCAACTTCCAGAA
CGTGAAGCAGCTGTACGCGCTGGTGTGCGAAACGCAGCGCTACTCCGCCGTGCTGGATGC
TGTGATCGCCAGCGCCGGCCTCCTCCGTGCGGAGAAGAAGCTGCGGCCGCACCTGGCCAA
GGTGCTAGTGTATGAGTTGTTGTTGGGAAAGGGCTTTCGAGGGGGTGGGGGCCGATGGAA
GGCTCTGTTGGGCCGGCACCAGGCGAGGCTCAAGGCTGAGTTGGCTCGGCTCAAGGTTCA
TCGGGGTGTGAGCCGGAATGAGGACCTGTTGGAAGTGGGATCCAGGCCTGGTCCAGCCTC
CCAGCTGCCTCGATTTGTGCGTGTGAACACTCTCAAGACCTGCTCCGATGATGTAGTTGA
TTATTTCAAGAGACAAGGTTTCTCCTATCAGGGTCGGGCTTCCAGCCTCGATGACTTACG
AGCCCTCAAGGGGAAGCATTTTCTCCTGGACCCCTTGATGCCGGAGCTGCTGGTGTTTCC
CGCCCAGACAGATCTGCATGAACACCCACTGTACCGGGCCGGACACCTCATTCTGCAGGA
CAGGGCCAGCTGTCTCCCAGCCATGCTGCTGGACCCCCCGCCAGGCTCCCATGTCATCGA
TGCCTGTGCCGCCCCAGGCAATAAGACCAGTCACTTGGCTGCTCTTCTGAAGAACCAAGG
GAAGATCTTTGCCTTTGACCTGGATGCCAAGCGGCTGGCATCCATGGCCACGCTGCTGGC
CCGGGCTGGCGTCTCTTGCTGTGAACTGGCTGAGGAGGACTTCCTGGCGGTCTCCCCCTC
GGATCCACGCTACCATGAGGTCCACTACATCCTGCTGGATCCTTCCTGCAGTGGCTCGGG
TATGCCGAGCAGACAGCTGGAGGAGCCCGGGGCAGGCACACCTAGCCCGGTGCGTCTGCA
TGCCCTGGCAGGGTTCCAGCAGCGAGCCCTGTGCCACGCACTCACTTTCCCTTCCCTGCA
GCGGCTCGTCTACTCCACGTGCTCCCTCTGCCAGGAGGAGAATGAAGACGTGGTGCGAGA
TGCGCTGCAGCAGAACCCGGGCGCCTTCAGGCTAGCTCCCGCCCTGCCTGCCTGGCCCCA
CCGAGGCCTGAGCACGTTCCCGGGTGCCGAGCACTGCCTCCGGGCCTCCCCTGAGACCAC
ACTCAGCAGTGGCTTCTTCGTTGCTGTAATTGAACGGGTCGAGGTGCCAAGCTCAGCCTC
ACAGGCCAAAGCATCAGCACCAGAACGCACACCCAGCCCAGCCCCAAAGAGAAAGAAGAG
ACAGCAAAGAGCCGCAGCCGGTGCTTGCACACCGCCTTGCACAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT