CHST4 ORF clone pFN21AE1865

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Halotag ORF Clone pFN21AE1865

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Clone info.

Species

human

Clone Name

pFN21AE1865 Sequence (with vector) Restriction Map

Product ID

FHC10867

ORF Sequence

DNA sequence (1158 bp)
Protein sequence (386 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590497]

Gene

KIEE1865

Symbol

CHST4

Alias : GST3, GlcNAc6ST2, HECGLCNAC6ST, LSST

Description

carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4, transcript variant 1

Original Clone ID

cs02765

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1158 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 386 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10902.1 2.9e-171 100.0 CHST4 CCDS10918.1 9.1e-84 56.8 CHST6 CCDS10919.1 1.5e-83 54.1 CHST5 CCDS7913.1 6.5e-44 36.7 CHST1 CCDS7312.1 9.5e-40 33.1 CHST3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000863 44 357 PF00685 Sulfotransferase domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 8 KLLLFLVSQMAILALFFHMYSHN 30 PRIMARY 23 2 45 VLVLSSWRSGSSFVGQLFGQHPD 67 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005256012 395 217800,605294 56.8 94.0 XP_011521387 395 217800,605294 56.8 94.0 NP_067628 395 217800,605294 56.8 94.0 NP_078809 411 604817 54.1 97.4 NP_003645 411 603797 36.7 85.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1865 Download

>KIEE1865  1158 bp
ATGCTACTGCCTAAAAAAATGAAGCTCCTGCTGTTTCTGGTTTCCCAGATGGCCATCTTG
GCTCTATTCTTCCACATGTACAGCCACAACATCAGCTCCCTGTCTATGAAGGCACAGCCC
GAGCGCATGCACGTGCTGGTTCTGTCTTCCTGGCGCTCTGGCTCTTCTTTTGTGGGGCAG
CTTTTTGGGCAGCACCCAGATGTTTTCTACCTGATGGAGCCCGCCTGGCACGTGTGGATG
ACCTTCAAGCAGAGCACCGCCTGGATGCTGCACATGGCTGTGCGGGATCTGATACGGGCC
GTCTTCTTGTGCGACATGAGCGTCTTTGATGCCTACATGGAACCTGGTCCCCGGAGACAG
TCCAGCCTCTTTCAGTGGGAGAACAGCCGGGCCCTGTGTTCTGCACCTGCCTGTGACATC
ATCCCACAAGATGAAATCATCCCCCGGGCTCACTGCAGGCTCCTGTGCAGTCAACAGCCC
TTTGAGGTGGTGGAGAAGGCCTGCCGCTCCTACAGCCACGTGGTGCTCAAGGAGGTGCGC
TTCTTCAACCTGCAGTCCCTCTACCCGCTGCTGAAAGACCCCTCCCTCAACCTGCATATC
GTGCACCTGGTCCGGGACCCCCGGGCCGTGTTCCGTTCCCGAGAACGCACAAAGGGAGAT
CTCATGATTGACAGTCGCATTGTGATGGGGCAGCATGAGCAAAAACTCAAGAAGGAGGAC
CAACCCTACTATGTGATGCAGGTCATCTGCCAAAGCCAGCTGGAGATCTACAAGACCATC
CAGTCCTTGCCCAAGGCCCTGCAGGAACGCTACCTGCTTGTGCGCTATGAGGACCTGGCT
CGAGCCCCTGTGGCCCAGACTTCCCGAATGTATGAATTCGTGGGATTGGAATTCTTGCCC
CATCTTCAGACCTGGGTGCATAACATCACCCGAGGCAAGGGCATGGGTGACCACGCTTTC
CACACAAATGCCAGGGATGCCCTTAATGTCTCCCAGGCTTGGCGCTGGTCTTTGCCCTAT
GAAAAGGTTTCTCGACTTCAGAAAGCCTGTGGCGATGCCATGAATTTGCTGGGCTACCGC
CACGTCAGATCTGAACAAGAACAGAGAAACCTGTTGCTGGATCTTCTGTCTACCTGGACT
GTCCCTGAGCAAATCCAC

ORF Protein

Cloned ORF protein sequence for pFN21AE1865 Download

>KIEE1865  386 aa
MLLPKKMKLLLFLVSQMAILALFFHMYSHNISSLSMKAQPERMHVLVLSSWRSGSSFVGQ
LFGQHPDVFYLMEPAWHVWMTFKQSTAWMLHMAVRDLIRAVFLCDMSVFDAYMEPGPRRQ
SSLFQWENSRALCSAPACDIIPQDEIIPRAHCRLLCSQQPFEVVEKACRSYSHVVLKEVR
FFNLQSLYPLLKDPSLNLHIVHLVRDPRAVFRSRERTKGDLMIDSRIVMGQHEQKLKKED
QPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLP
HLQTWVHNITRGKGMGDHAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYR
HVRSEQEQRNLLLDLLSTWTVPEQIH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1865 Download

>pFN21AE1865  5863 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTACTGCCTAAAAAAATGAAGCTCCTGCTGTT
TCTGGTTTCCCAGATGGCCATCTTGGCTCTATTCTTCCACATGTACAGCCACAACATCAG
CTCCCTGTCTATGAAGGCACAGCCCGAGCGCATGCACGTGCTGGTTCTGTCTTCCTGGCG
CTCTGGCTCTTCTTTTGTGGGGCAGCTTTTTGGGCAGCACCCAGATGTTTTCTACCTGAT
GGAGCCCGCCTGGCACGTGTGGATGACCTTCAAGCAGAGCACCGCCTGGATGCTGCACAT
GGCTGTGCGGGATCTGATACGGGCCGTCTTCTTGTGCGACATGAGCGTCTTTGATGCCTA
CATGGAACCTGGTCCCCGGAGACAGTCCAGCCTCTTTCAGTGGGAGAACAGCCGGGCCCT
GTGTTCTGCACCTGCCTGTGACATCATCCCACAAGATGAAATCATCCCCCGGGCTCACTG
CAGGCTCCTGTGCAGTCAACAGCCCTTTGAGGTGGTGGAGAAGGCCTGCCGCTCCTACAG
CCACGTGGTGCTCAAGGAGGTGCGCTTCTTCAACCTGCAGTCCCTCTACCCGCTGCTGAA
AGACCCCTCCCTCAACCTGCATATCGTGCACCTGGTCCGGGACCCCCGGGCCGTGTTCCG
TTCCCGAGAACGCACAAAGGGAGATCTCATGATTGACAGTCGCATTGTGATGGGGCAGCA
TGAGCAAAAACTCAAGAAGGAGGACCAACCCTACTATGTGATGCAGGTCATCTGCCAAAG
CCAGCTGGAGATCTACAAGACCATCCAGTCCTTGCCCAAGGCCCTGCAGGAACGCTACCT
GCTTGTGCGCTATGAGGACCTGGCTCGAGCCCCTGTGGCCCAGACTTCCCGAATGTATGA
ATTCGTGGGATTGGAATTCTTGCCCCATCTTCAGACCTGGGTGCATAACATCACCCGAGG
CAAGGGCATGGGTGACCACGCTTTCCACACAAATGCCAGGGATGCCCTTAATGTCTCCCA
GGCTTGGCGCTGGTCTTTGCCCTATGAAAAGGTTTCTCGACTTCAGAAAGCCTGTGGCGA
TGCCATGAATTTGCTGGGCTACCGCCACGTCAGATCTGAACAAGAACAGAGAAACCTGTT
GCTGGATCTTCTGTCTACCTGGACTGTCCCTGAGCAAATCCACGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT