KHSRP ORF clone pFN21AE2201

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2201

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Clone info.

Species

human

Clone Name

pFN21AE2201 Sequence (with vector) Restriction Map

Product ID

FHC30784

ORF Sequence

DNA sequence (2133 bp)
Protein sequence (711 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2201

Symbol

KHSRP

Alias : FBP2, FUBP2, KSRP, p75

Description

KH-type splicing regulatory protein

Original Clone ID

cs03301

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2133 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 711 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45936.1 5e-113 100.0 KHSRP CCDS683.1 2.1e-48 63.3 FUBP1 CCDS43893.1 1.6e-31 49.1 FUBP3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018111 146 208 PF00013 K Homology IPR018111 236 299 PF00013 K Homology IPR018111 326 385 PF00013 K Homology IPR018111 427 491 PF00013 K Homology IPR015096 611 632 PF09005 Domain of unknown function DUF1897 IPR015096 666 692 PF09005 Domain of unknown function DUF1897 HMMSmart IPR004087 143 213 SM00322 K Homology IPR004087 232 304 SM00322 K Homology IPR004087 321 391 SM00322 K Homology IPR004087 423 496 SM00322 K Homology ProfileScan IPR004088 144 208 PS50084 K Homology IPR004088 233 299 PS50084 K Homology IPR004088 322 386 PS50084 K Homology IPR004088 424 491 PS50084 K Homology
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003676 711 603445 100.0 100.0 XP_005259725 747 603445 100.0 99.9 XP_011526697 703 603445 100.0 92.8 XP_016882897 678 603445 96.2 92.8 XP_016882898 642 603445 96.2 93.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2201 Download

>KIEE2201  2133 bp
ATGTCGGACTACAGCACGGGAGGACCCCCGCCCGGGCCGCCGCCGCCCGCCGGCGGGGGC
GGGGGAGCCGGAGGCGCCGGGGGAGGCCCTCCGCCGGGCCCGCCAGGCGCGGGGGACCGG
GGCGGCGGCGGTCCCGGCGGCGGCGGCCCGGGCGGGGGGTCGGCCGGGGGCCCCTCTCAG
CCACCCGGCGGAGGCGGCCCGGGAATCCGCAAGGACGCTTTCGCCGACGCCGTGCAGCGG
GCCCGCCAGATTGCAGCCAAAATTGGAGGCGATGCTGCCACGACAGTGAATAACAGCACT
CCTGATTTTGGTTTTGGGGGCCAAAAGAGACAGTTGGAAGATGGAGATCAACCGGAGAGC
AAGAAGCTGGCTTCCCAGGGAGACTCAATCAGTTCTCAACTTGGACCCATCCATCCTCCC
CCAAGGACTTCAATGACAGAAGAGTACAGGGTCCCAGACGGCATGGTGGGCCTGATCATT
GGCAGAGGAGGTGAACAAATTAACAAAATCCAACAGGATTCAGGCTGCAAAGTACAGATT
TCTCCAGACAGCGGTGGCCTACCCGAGCGCAGTGTGTCCTTGACAGGAGCCCCAGAATCT
GTCCAGAAAGCCAAGATGATGCTGGATGACATTGTGTCTCGGGGTCGTGGGGGCCCCCCA
GGACAGTTCCACGACAACGCCAACGGGGGCCAGAACGGCACCGTGCAGGAGATCATGATC
CCCGCGGGCAAGGCCGGCCTGGTCATTGGCAAGGGCGGGGAGACCATTAAGCAGCTGCAG
GAACGCGCTGGAGTGAAGATGATCTTAATTCAGGACGGATCTCAGAATACGAATGTGGAC
AAACCTCTCCGCATCATTGGGGATCCTTACAAAGTGCAGCAAGCCTGTGAGATGGTGATG
GACATCCTCCGGGAACGTGACCAAGGCGGCTTTGGGGACCGGAATGAGTACGGATCTCGG
ATTGGCGGAGGCATCGATGTGCCAGTGCCCAGGCATTCTGTTGGCGTGGTCATTGGCCGG
AGTGGAGAGATGATCAAGAAGATCCAGAATGATGCTGGCGTGCGGATACAGTTCAAGCAA
GATGACGGGACAGGGCCCGAGAAGATTGCTCATATAATGGGGCCCCCAGACAGGTGCGAG
CACGCAGCCCGGATCATCAACGACCTCCTCCAGAGCCTCAGGAGTGGTCCCCCAGGTCCT
CCAGGGGGTCCAGGCATGCCCCCGGGGGGCCGAGGCCGAGGAAGAGGCCAAGGCAATTGG
GGTCCCCCTGGCGGGGAGATGACCTTCTCCATCCCCACTCACAAGTGTGGGCTGGTCATC
GGCCGAGGTGGCGAGAATGTGAAAGCCATAAACCAGCAGACGGGAGCCTTCGTAGAGATC
TCCCGGCAGCTGCCACCCAACGGGGACCCCAACTTCAAGTTGTTCATCATCCGGGGTTCA
CCCCAGCAGATTGACCACGCCAAGCAGCTTATCGAGGAAAAGATCGAGGGTCCTCTCTGC
CCAGTTGGACCAGGCCCAGGTGGCCCAGGCCCTGCTGGCCCAATGGGGCCCTTCAATCCT
GGGCCCTTCAACCAGGGGCCACCCGGGGCTCCCCCACATGCCGGGGGGCCCCCTCCTCAC
CAGTACCCACCCCAGGGCTGGGGCAATACCTACCCCCAGTGGCAGCCGCCTGCTCCTCAT
GACCCAAGCAAAGCAGCTGCAGCGGCCGCGGACCCCAACGCCGCGTGGGCCGCCTACTAC
TCACACTACTACCAGCAGCCCCCGGGCCCCGTCCCCGGCCCCGCACCGGCCCCTGCGGCC
CCACCGGCTCAGGGTGAGCCCCCTCAGCCCCCACCCACCGGCCAGTCGGACTACACTAAG
GCCTGGGAAGAGTATTACAAAAAGATCGGCCAGCAGCCCCAGCAGCCCGGAGCGCCCCCA
CAGCAGGACTACACGAAGGCTTGGGAGGAGTACTACAAGAAGCAAGCGCAAGTGGCCACC
GGAGGGGGTCCAGGAGCTCCCCCAGGCTCCCAGCCAGACTACAGTGCCGCCTGGGCGGAA
TATTACAGACAGCAGGCCGCTTACTACGGACAGACCCCAGGTCCTGGCGGCCCCCAGCCG
CCGCCCACGCAGCAGGGACAGCAGCAGGCTCAA

ORF Protein

Cloned ORF protein sequence for pFN21AE2201 Download

>KIEE2201  711 aa
MSDYSTGGPPPGPPPPAGGGGGAGGAGGGPPPGPPGAGDRGGGGPGGGGPGGGSAGGPSQ
PPGGGGPGIRKDAFADAVQRARQIAAKIGGDAATTVNNSTPDFGFGGQKRQLEDGDQPES
KKLASQGDSISSQLGPIHPPPRTSMTEEYRVPDGMVGLIIGRGGEQINKIQQDSGCKVQI
SPDSGGLPERSVSLTGAPESVQKAKMMLDDIVSRGRGGPPGQFHDNANGGQNGTVQEIMI
PAGKAGLVIGKGGETIKQLQERAGVKMILIQDGSQNTNVDKPLRIIGDPYKVQQACEMVM
DILRERDQGGFGDRNEYGSRIGGGIDVPVPRHSVGVVIGRSGEMIKKIQNDAGVRIQFKQ
DDGTGPEKIAHIMGPPDRCEHAARIINDLLQSLRSGPPGPPGGPGMPPGGRGRGRGQGNW
GPPGGEMTFSIPTHKCGLVIGRGGENVKAINQQTGAFVEISRQLPPNGDPNFKLFIIRGS
PQQIDHAKQLIEEKIEGPLCPVGPGPGGPGPAGPMGPFNPGPFNQGPPGAPPHAGGPPPH
QYPPQGWGNTYPQWQPPAPHDPSKAAAAAADPNAAWAAYYSHYYQQPPGPVPGPAPAPAA
PPAQGEPPQPPPTGQSDYTKAWEEYYKKIGQQPQQPGAPPQQDYTKAWEEYYKKQAQVAT
GGGPGAPPGSQPDYSAAWAEYYRQQAAYYGQTPGPGGPQPPPTQQGQQQAQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2201 Download

>pFN21AE2201  6838 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGGACTACAGCACGGGAGGACCCCCGCCCGG
GCCGCCGCCGCCCGCCGGCGGGGGCGGGGGAGCCGGAGGCGCCGGGGGAGGCCCTCCGCC
GGGCCCGCCAGGCGCGGGGGACCGGGGCGGCGGCGGTCCCGGCGGCGGCGGCCCGGGCGG
GGGGTCGGCCGGGGGCCCCTCTCAGCCACCCGGCGGAGGCGGCCCGGGAATCCGCAAGGA
CGCTTTCGCCGACGCCGTGCAGCGGGCCCGCCAGATTGCAGCCAAAATTGGAGGCGATGC
TGCCACGACAGTGAATAACAGCACTCCTGATTTTGGTTTTGGGGGCCAAAAGAGACAGTT
GGAAGATGGAGATCAACCGGAGAGCAAGAAGCTGGCTTCCCAGGGAGACTCAATCAGTTC
TCAACTTGGACCCATCCATCCTCCCCCAAGGACTTCAATGACAGAAGAGTACAGGGTCCC
AGACGGCATGGTGGGCCTGATCATTGGCAGAGGAGGTGAACAAATTAACAAAATCCAACA
GGATTCAGGCTGCAAAGTACAGATTTCTCCAGACAGCGGTGGCCTACCCGAGCGCAGTGT
GTCCTTGACAGGAGCCCCAGAATCTGTCCAGAAAGCCAAGATGATGCTGGATGACATTGT
GTCTCGGGGTCGTGGGGGCCCCCCAGGACAGTTCCACGACAACGCCAACGGGGGCCAGAA
CGGCACCGTGCAGGAGATCATGATCCCCGCGGGCAAGGCCGGCCTGGTCATTGGCAAGGG
CGGGGAGACCATTAAGCAGCTGCAGGAACGCGCTGGAGTGAAGATGATCTTAATTCAGGA
CGGATCTCAGAATACGAATGTGGACAAACCTCTCCGCATCATTGGGGATCCTTACAAAGT
GCAGCAAGCCTGTGAGATGGTGATGGACATCCTCCGGGAACGTGACCAAGGCGGCTTTGG
GGACCGGAATGAGTACGGATCTCGGATTGGCGGAGGCATCGATGTGCCAGTGCCCAGGCA
TTCTGTTGGCGTGGTCATTGGCCGGAGTGGAGAGATGATCAAGAAGATCCAGAATGATGC
TGGCGTGCGGATACAGTTCAAGCAAGATGACGGGACAGGGCCCGAGAAGATTGCTCATAT
AATGGGGCCCCCAGACAGGTGCGAGCACGCAGCCCGGATCATCAACGACCTCCTCCAGAG
CCTCAGGAGTGGTCCCCCAGGTCCTCCAGGGGGTCCAGGCATGCCCCCGGGGGGCCGAGG
CCGAGGAAGAGGCCAAGGCAATTGGGGTCCCCCTGGCGGGGAGATGACCTTCTCCATCCC
CACTCACAAGTGTGGGCTGGTCATCGGCCGAGGTGGCGAGAATGTGAAAGCCATAAACCA
GCAGACGGGAGCCTTCGTAGAGATCTCCCGGCAGCTGCCACCCAACGGGGACCCCAACTT
CAAGTTGTTCATCATCCGGGGTTCACCCCAGCAGATTGACCACGCCAAGCAGCTTATCGA
GGAAAAGATCGAGGGTCCTCTCTGCCCAGTTGGACCAGGCCCAGGTGGCCCAGGCCCTGC
TGGCCCAATGGGGCCCTTCAATCCTGGGCCCTTCAACCAGGGGCCACCCGGGGCTCCCCC
ACATGCCGGGGGGCCCCCTCCTCACCAGTACCCACCCCAGGGCTGGGGCAATACCTACCC
CCAGTGGCAGCCGCCTGCTCCTCATGACCCAAGCAAAGCAGCTGCAGCGGCCGCGGACCC
CAACGCCGCGTGGGCCGCCTACTACTCACACTACTACCAGCAGCCCCCGGGCCCCGTCCC
CGGCCCCGCACCGGCCCCTGCGGCCCCACCGGCTCAGGGTGAGCCCCCTCAGCCCCCACC
CACCGGCCAGTCGGACTACACTAAGGCCTGGGAAGAGTATTACAAAAAGATCGGCCAGCA
GCCCCAGCAGCCCGGAGCGCCCCCACAGCAGGACTACACGAAGGCTTGGGAGGAGTACTA
CAAGAAGCAAGCGCAAGTGGCCACCGGAGGGGGTCCAGGAGCTCCCCCAGGCTCCCAGCC
AGACTACAGTGCCGCCTGGGCGGAATATTACAGACAGCAGGCCGCTTACTACGGACAGAC
CCCAGGTCCTGGCGGCCCCCAGCCGCCGCCCACGCAGCAGGGACAGCAGCAGGCTCAAGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT