SH2D3C ORF clone pFN21AE3508

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3508

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Clone info.

Species

human

Clone Name

pFN21AE3508 Sequence (with vector) Restriction Map

Product ID

FHC11653

ORF Sequence

DNA sequence (2109 bp)
Protein sequence (703 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3508

Symbol

SH2D3C

Alias : CHAT, NSP3, PRO34088, SHEP1

Description

SH2 domain containing 3C, transcript variant 2

Original Clone ID

cp93508

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2109 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 703 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6878.1 1.7e-149 100.0 SH2D3C CCDS59145.1 4.6e-147 99.4 SH2D3C CCDS6877.1 5.2e-146 99.7 SH2D3C CCDS48027.1 8e-144 97.6 SH2D3C CCDS48026.1 2e-143 99.9 SH2D3C The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000980 63 77 PR00401 SH2 motif IPR000980 80 90 PR00401 SH2 motif IPR000980 104 114 PR00401 SH2 motif IPR000980 132 146 PR00401 SH2 motif HMMPfam IPR000980 63 143 PF00017 SH2 motif IPR001895 429 556 PF00617 Guanine-nucleotide dissociation stimulator CDC25 HMMSmart IPR000980 61 149 SM00252 SH2 motif IPR001895 425 698 SM00147 Guanine-nucleotide dissociation stimulator CDC25 ProfileScan IPR000980 63 162 PS50001 SH2 motif IPR001895 429 697 PS50009 Guanine-nucleotide dissociation stimulator CDC25
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005480 703 604722 100.0 100.0 NP_001239263 792 604722 99.4 99.1 XP_011516417 804 604722 99.7 98.3 XP_011516416 804 604722 99.7 98.3 NP_733745 860 604722 99.7 98.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3508 Download

>KIEE3508  2109 bp
ATGACTGCTGTGGGCCGAAGGTGCCCCGCGCTGGGGTCCCGAGGGGCTGCTGGAGAGCCA
GAGGCTGGCAGCGACTATGTGAAGTTCTCCAAGGAGAAGTACATCCTGGACTCATCGCCA
GAGAAACTCCACAAGGAATTGGAGGAGGAGCTCAAACTCAGCAGCACGGATCTCCGCAGC
CATGCCTGGTACCATGGCCGCATCCCCCGAGAGGTCTCGGAGACCTTGGTACAACGCAAC
GGCGACTTCCTCATCCGGGACTCACTCACCAGCCTGGGCGACTATGTGCTCACGTGCCGC
TGGCGCAACCAGGCCTTGCACTTCAAGATCAACAAGGTGGTGGTGAAGGCAGGCGAGAGC
TACACACACATCCAGTACCTGTTTGAGCAGGAGAGCTTTGACCACGTGCCCGCCCTCGTG
CGCTATCATGTGGGCAGCCGCAAGGCTGTGTCAGAGCAGAGTGGTGCCATCATCTACTGC
CCGGTGAACCGCACCTTCCCACTGCGCTACCTCGAGGCCAGCTATGGCCTGGGACAGGGG
AGTAGCAAGCCTGCTAGCCCCGTCAGCCCCTCAGGCCCCAAGGGCAGCCACATGAAGCGG
CGCAGCGTCACCATGACCGATGGGCTCACTGCTGACAAGGTCACCCGCAGCGATGGCTGC
CCCACCAGTACGTCGCTGCCCCGCCCTCGGGACTCCATCCGCAGCTGTGCCCTCAGCATG
GACCAGATCCCAGACCTGCACTCACCCATGTCGCCCATCTCCGAGAGCCCTAGCTCCCCT
GCCTACAGCACTGTAACCCGTGTCCATGCCGCCCCTGCAGCCCCTTCTGCCACAGCATTG
CCTGCCTCCCCTGTCGCCCGCCGTTCCAGTGAGCCCCAGCTGTGTCCCGGAAGTGCCCCA
AAGACCCATGGGGAGTCAGACAAGGGCCCCCACACCAGCCCCTCCCACACCCTTGGCAAG
GCCTCCCCGTCACCATCACTCAGCAGCTACAGTGACCCGGACTCTGGCCACTACTGCCAG
CTCCAGCCTCCCGTGCGTGGCAGCCGAGAGTGGGCAGCGACTGAGACCTCCAGCCAGCAG
GCCAGGAGCTATGGGGAGAGGCTAAAGGAACTGTCAGAAAATGGGGCCCCTGAAGGGGAC
TGGGGCAAGACCTTCACAGTCCCCATCGTGGAAGTCACTTCTTCCTTCAACCCGGCCACC
TTCCAGTCACTACTGATCCCCAGGGATAACCGGCCACTGGAGGTGGGCCTTCTGCGCAAG
GTCAAGGAGCTGCTGGCAGAAGTGGATGCCCGGACGCTGGCCCGGCATGTCACCAAGGTG
GACTGCCTGGTTGCTAGGATACTGGGCGTTACCAAGGAGATGCAGACCCTAATGGGAGTC
CGCTGGGGCATGGAACTGCTCACCCTCCCCCATGGCCGGCAGCTACGCCTAGACCTGCTG
GAAAGGTTCCACACCATGTCCATCATGCTGGCCGTGGACATCCTGGGCTGCACCGGCTCT
GCGGAGGAGCGGGCAGCGCTGCTGCACAAGACCATTCAGCTGGCGGCCGAGCTGCGGGGG
ACTATGGGCAACATGTTCAGCTTCGCGGCGGTCATGGGTGCCCTGGACATGGCTCAGATT
TCTCGGCTGGAGCAGACATGGGTGACCCTGCGGCAGCGACACACAGAGGGTGCCATCCTG
TACGAGAAGAAGCTCAAGCCTTTTCTCAAGAGCCTCAACGAGGGCAAAGAAGGCCCGCCG
CTGAGCAACACCACGTTTCCTCATGTGCTGCCCCTCATCACCCTGCTGGAGTGTGACTCG
GCCCCACCAGAGGGCCCTGAGCCCTGGGGCAGCACGGAGCACGGCGTGGAGGTGGTGCTG
GCTCACCTGGAGGCCGCCCGCACAGTGGCACACCACGGAGGCCTGTACCACACCAATGCT
GAAGTCAAGCTGCAGGGGTTCCAGGCCCGGCCGGAGCTCCTGGAGGTGTTCAGCACGGAG
TTCCAGATGCGCCTTCTCTGGGGCAGTCAGGGTGCCAGCAGCAGCCAGGCCCGGCGCTAT
GAGAAGTTCGACAAGGTCCTCACTGCCCTGTCCCACAAGCTGGAACCTGCTGTCCGCTCC
AGCGAGCTG

ORF Protein

Cloned ORF protein sequence for pFN21AE3508 Download

>KIEE3508  703 aa
MTAVGRRCPALGSRGAAGEPEAGSDYVKFSKEKYILDSSPEKLHKELEEELKLSSTDLRS
HAWYHGRIPREVSETLVQRNGDFLIRDSLTSLGDYVLTCRWRNQALHFKINKVVVKAGES
YTHIQYLFEQESFDHVPALVRYHVGSRKAVSEQSGAIIYCPVNRTFPLRYLEASYGLGQG
SSKPASPVSPSGPKGSHMKRRSVTMTDGLTADKVTRSDGCPTSTSLPRPRDSIRSCALSM
DQIPDLHSPMSPISESPSSPAYSTVTRVHAAPAAPSATALPASPVARRSSEPQLCPGSAP
KTHGESDKGPHTSPSHTLGKASPSPSLSSYSDPDSGHYCQLQPPVRGSREWAATETSSQQ
ARSYGERLKELSENGAPEGDWGKTFTVPIVEVTSSFNPATFQSLLIPRDNRPLEVGLLRK
VKELLAEVDARTLARHVTKVDCLVARILGVTKEMQTLMGVRWGMELLTLPHGRQLRLDLL
ERFHTMSIMLAVDILGCTGSAEERAALLHKTIQLAAELRGTMGNMFSFAAVMGALDMAQI
SRLEQTWVTLRQRHTEGAILYEKKLKPFLKSLNEGKEGPPLSNTTFPHVLPLITLLECDS
APPEGPEPWGSTEHGVEVVLAHLEAARTVAHHGGLYHTNAEVKLQGFQARPELLEVFSTE
FQMRLLWGSQGASSSQARRYEKFDKVLTALSHKLEPAVRSSEL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3508 Download

>pFN21AE3508  6814 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACTGCTGTGGGCCGAAGGTGCCCCGCGCTGGG
GTCCCGAGGGGCTGCTGGAGAGCCAGAGGCTGGCAGCGACTATGTGAAGTTCTCCAAGGA
GAAGTACATCCTGGACTCATCGCCAGAGAAACTCCACAAGGAATTGGAGGAGGAGCTCAA
ACTCAGCAGCACGGATCTCCGCAGCCATGCCTGGTACCATGGCCGCATCCCCCGAGAGGT
CTCGGAGACCTTGGTACAACGCAACGGCGACTTCCTCATCCGGGACTCACTCACCAGCCT
GGGCGACTATGTGCTCACGTGCCGCTGGCGCAACCAGGCCTTGCACTTCAAGATCAACAA
GGTGGTGGTGAAGGCAGGCGAGAGCTACACACACATCCAGTACCTGTTTGAGCAGGAGAG
CTTTGACCACGTGCCCGCCCTCGTGCGCTATCATGTGGGCAGCCGCAAGGCTGTGTCAGA
GCAGAGTGGTGCCATCATCTACTGCCCGGTGAACCGCACCTTCCCACTGCGCTACCTCGA
GGCCAGCTATGGCCTGGGACAGGGGAGTAGCAAGCCTGCTAGCCCCGTCAGCCCCTCAGG
CCCCAAGGGCAGCCACATGAAGCGGCGCAGCGTCACCATGACCGATGGGCTCACTGCTGA
CAAGGTCACCCGCAGCGATGGCTGCCCCACCAGTACGTCGCTGCCCCGCCCTCGGGACTC
CATCCGCAGCTGTGCCCTCAGCATGGACCAGATCCCAGACCTGCACTCACCCATGTCGCC
CATCTCCGAGAGCCCTAGCTCCCCTGCCTACAGCACTGTAACCCGTGTCCATGCCGCCCC
TGCAGCCCCTTCTGCCACAGCATTGCCTGCCTCCCCTGTCGCCCGCCGTTCCAGTGAGCC
CCAGCTGTGTCCCGGAAGTGCCCCAAAGACCCATGGGGAGTCAGACAAGGGCCCCCACAC
CAGCCCCTCCCACACCCTTGGCAAGGCCTCCCCGTCACCATCACTCAGCAGCTACAGTGA
CCCGGACTCTGGCCACTACTGCCAGCTCCAGCCTCCCGTGCGTGGCAGCCGAGAGTGGGC
AGCGACTGAGACCTCCAGCCAGCAGGCCAGGAGCTATGGGGAGAGGCTAAAGGAACTGTC
AGAAAATGGGGCCCCTGAAGGGGACTGGGGCAAGACCTTCACAGTCCCCATCGTGGAAGT
CACTTCTTCCTTCAACCCGGCCACCTTCCAGTCACTACTGATCCCCAGGGATAACCGGCC
ACTGGAGGTGGGCCTTCTGCGCAAGGTCAAGGAGCTGCTGGCAGAAGTGGATGCCCGGAC
GCTGGCCCGGCATGTCACCAAGGTGGACTGCCTGGTTGCTAGGATACTGGGCGTTACCAA
GGAGATGCAGACCCTAATGGGAGTCCGCTGGGGCATGGAACTGCTCACCCTCCCCCATGG
CCGGCAGCTACGCCTAGACCTGCTGGAAAGGTTCCACACCATGTCCATCATGCTGGCCGT
GGACATCCTGGGCTGCACCGGCTCTGCGGAGGAGCGGGCAGCGCTGCTGCACAAGACCAT
TCAGCTGGCGGCCGAGCTGCGGGGGACTATGGGCAACATGTTCAGCTTCGCGGCGGTCAT
GGGTGCCCTGGACATGGCTCAGATTTCTCGGCTGGAGCAGACATGGGTGACCCTGCGGCA
GCGACACACAGAGGGTGCCATCCTGTACGAGAAGAAGCTCAAGCCTTTTCTCAAGAGCCT
CAACGAGGGCAAAGAAGGCCCGCCGCTGAGCAACACCACGTTTCCTCATGTGCTGCCCCT
CATCACCCTGCTGGAGTGTGACTCGGCCCCACCAGAGGGCCCTGAGCCCTGGGGCAGCAC
GGAGCACGGCGTGGAGGTGGTGCTGGCTCACCTGGAGGCCGCCCGCACAGTGGCACACCA
CGGAGGCCTGTACCACACCAATGCTGAAGTCAAGCTGCAGGGGTTCCAGGCCCGGCCGGA
GCTCCTGGAGGTGTTCAGCACGGAGTTCCAGATGCGCCTTCTCTGGGGCAGTCAGGGTGC
CAGCAGCAGCCAGGCCCGGCGCTATGAGAAGTTCGACAAGGTCCTCACTGCCCTGTCCCA
CAAGCTGGAACCTGCTGTCCGCTCCAGCGAGCTGGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT