NGEF ORF clone pFN21AE3911

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Halotag ORF Clone pFN21AE3911

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Clone info.

Species

human

Clone Name

pFN21AE3911 Sequence (with vector) Restriction Map

Product ID

FHC09378

Flexi Clone PID

FXC09378

ORF Sequence

DNA sequence (2130 bp)
Protein sequence (710 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3911

Symbol

NGEF

Alias : ARHGEF27, EPHEXIN

Description

neuronal guanine nucleotide exchange factor, transcript variant 1

Original Clone ID

cp93911

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2130 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 710 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2500.1 4.8e-183 99.9 NGEF CCDS46544.1 1.3e-148 98.5 NGEF CCDS170.1 3.6e-43 35.3 ARHGEF19 CCDS34771.1 3.6e-43 38.9 ARHGEF5 CCDS46.2 7e-38 32.2 ARHGEF16 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000219 278 456 PF00621 Dbl homology (DH) domain IPR001452 621 665 PF00018 Src homology-3 domain HMMSmart IPR000219 277 456 SM00325 Dbl homology (DH) domain IPR001849 490 603 SM00233 Pleckstrin homology domain IPR001452 615 672 SM00326 Src homology-3 domain ProfileScan IPR000219 273 457 PS50010 Dbl homology (DH) domain IPR001849 489 601 PS50003 Pleckstrin homology domain IPR001452 612 673 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011509225 710 605991 99.9 100.0 NP_062824 710 605991 99.9 100.0 NP_001107562 618 605991 98.5 83.7 NP_694945 802 612496 35.3 92.4 XP_011539008 802 612496 35.3 92.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3911 Download

>KIEE3911  2130 bp
ATGGAGACCAGGGAATCTGAAGATTTGGAAAAGACCCGGAGGAAATCAGCAAGTGATCAA
TGGAACACTGATAATGAACCAGCCAAGGTGAAACCTGAGTTACTCCCAGAAAAAGAGGAG
ACTTCTCAAGCTGACCAGGATATCCAAGACAAAGAGCCTCATTGCCACATCCCAATTAAG
AGAAATTCCATCTTCAATCGCTCCATAAGACGCAAAAGCAAAGCCAAGGCCAGAGACAAC
CCCGAACGGAACGCCAGCTGCCTGGCAGATTCACAGGACAATGGAAAATCTGTAAATGAG
CCCCTGACCTTGAATATCCCCTGGAGCAGAACGCCTCCTTGCAGAACAGCAATGCAGACA
GACCCAGGAGCCCAGGAAATGAGTGAGTCGTCCTCCACCCCGGGAAATGGGGCCACGCCC
GAGGAGTGGCCGGCCCTGGCCGACAGCCCCACCACGCTCACCGAGGCCCTGCGGATGATC
CACCCCATTCCCGCCGACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAG
GAATACCGAGATAAATCGACTCTCCAAGAAATCGAAACCAGGAGGCAACAGGATGCAGAA
ATAGAAGACAATACCAATGGGTCCCCGGCCAGTGAGGACACCCCGGAGGAGGAAGAAGAA
GAGGAGGAGGAGGAGGAGCCGGCCAGCCCACCAGAGAGGAAGACTCTGCCCCAGATCTGC
CTGCTCAGTAACCCCCACTCAAGGTTCAACCTCTGGCAGGATCTTCCCGAGATCCGGAGC
AGCGGGGTGCTTGAGATCCTACAGCCTGAGGAGATTAAGCTGCAGGAGGCCATGTTCGAG
CTGGTCACTTCCGAGGCGTCCTACTACAAGAGTCTGAACCTGCTCGTGTCCCACTTCATG
GAGAACGAGCGGATAAGGAAGATCCTGCACCCGTCCGAGGCGCACATCCTCTTCTCCAAC
GTCCTGGACGTGCTGGCTGTCAGTGAGCGGTTCCTCCTGGAGCTGGAGCACCGGATGGAG
GAGAACATCGTCATCTCTGACGTGTGTGACATCGTGTACCGTTATGCGGCTGACCACTTC
TCTGTCTACATCACCTACGTCAGCAATCAGACCTACCAGGAGCGGACCTATAAGCAGCTG
CTCCAGGAGAAGGCAGCTTTCCGGGAGCTGATCGCGCAGCTAGAGCTCGACCCCAAGTGC
AGGGGGCTGCCCTTCTCCTCCTTCCTCATCCTGCCTTTCCAGAGGATCACACGCCTCAAG
CTGTTGGTCCAGAACATCCTGAAGAGGGTAGAAGAGAGGTCTGAGCGGGAGTGCACTGCT
TTGGATGCTCACAAGGAGCTGGAAATGGTGGTGAAGGCATGCAACGAGGGCGTCAGGAAA
ATGAGCCGCACGGAACAGATGATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCG
GTGCCCATCATCTCCCACTCCCGCTGGCTGCTGAAGCAGGGTGAGCTGCAGCAGATGTCA
GGCCCCAAGACCTCCCGGACCCTGAGGACCAAGAAGCTCTTCCACGAAATTTACCTCTTC
CTGTTCAACGACCTGCTGGTGATCTGCCGGCAGATTCCAGGAGACAAGTACCAGGTATTT
GACTCAGCTCCGCGGGGACTGCTGCGTGTGGAGGAGCTGGAGGACCAGGGCCAGACGCTG
GCCAACGTGTTCATCCTGCGGCTGCTGGAGAACGCAGATGACCGGGAGGCCACCTACATG
CTAAAGGCGTCCTCTCAGAGTGAGATGAAGCGTTGGATGACCTCACTGGCCCCCAACAGG
AGGACCAAGTTTGTTTCGTTCACATCCCGGCTGCTGGACTGCCCCCAGGTCCAGTGCGTG
CACCCATACGTGGCTCAGCAGCCAGACGAGCTGACGCTGGAGCTCGCCGACATCCTCAAC
ATCCTGGACAAGACTGACGACGGGTGGATCTTTGGCGAGCGTCTGCACGACCAGGAGAGA
GGCTGGTTCCCCAGCTCCATGACTGAGGAGATCTTGAATCCCAAGATCCGGTCCCAGAAC
CTCAAGGAATGTTTCCGTGTCCACAAGATGGATGACCCTCAGCGCAGCCAGAACAAGGAC
CGCAGGAAGCTGGGCAGCCGGAATCGGCAA

ORF Protein

Cloned ORF protein sequence for pFN21AE3911 Download

>KIEE3911  710 aa
METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIK
RNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRTPPCRTAMQT
DPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQ
EYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQIC
LLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFM
ENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHF
SVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLK
LLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKS
VPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVF
DSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNR
RTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQER
GWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3911 Download

>pFN21AE3911  6835 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGACCAGGGAATCTGAAGATTTGGAAAAGAC
CCGGAGGAAATCAGCAAGTGATCAATGGAACACTGATAATGAACCAGCCAAGGTGAAACC
TGAGTTACTCCCAGAAAAAGAGGAGACTTCTCAAGCTGACCAGGATATCCAAGACAAAGA
GCCTCATTGCCACATCCCAATTAAGAGAAATTCCATCTTCAATCGCTCCATAAGACGCAA
AAGCAAAGCCAAGGCCAGAGACAACCCCGAACGGAACGCCAGCTGCCTGGCAGATTCACA
GGACAATGGAAAATCTGTAAATGAGCCCCTGACCTTGAATATCCCCTGGAGCAGAACGCC
TCCTTGCAGAACAGCAATGCAGACAGACCCAGGAGCCCAGGAAATGAGTGAGTCGTCCTC
CACCCCGGGAAATGGGGCCACGCCCGAGGAGTGGCCGGCCCTGGCCGACAGCCCCACCAC
GCTCACCGAGGCCCTGCGGATGATCCACCCCATTCCCGCCGACTCCTGGAGAAACCTCAT
TGAACAAATAGGGCTCCTGTATCAGGAATACCGAGATAAATCGACTCTCCAAGAAATCGA
AACCAGGAGGCAACAGGATGCAGAAATAGAAGACAATACCAATGGGTCCCCGGCCAGTGA
GGACACCCCGGAGGAGGAAGAAGAAGAGGAGGAGGAGGAGGAGCCGGCCAGCCCACCAGA
GAGGAAGACTCTGCCCCAGATCTGCCTGCTCAGTAACCCCCACTCAAGGTTCAACCTCTG
GCAGGATCTTCCCGAGATCCGGAGCAGCGGGGTGCTTGAGATCCTACAGCCTGAGGAGAT
TAAGCTGCAGGAGGCCATGTTCGAGCTGGTCACTTCCGAGGCGTCCTACTACAAGAGTCT
GAACCTGCTCGTGTCCCACTTCATGGAGAACGAGCGGATAAGGAAGATCCTGCACCCGTC
CGAGGCGCACATCCTCTTCTCCAACGTCCTGGACGTGCTGGCTGTCAGTGAGCGGTTCCT
CCTGGAGCTGGAGCACCGGATGGAGGAGAACATCGTCATCTCTGACGTGTGTGACATCGT
GTACCGTTATGCGGCTGACCACTTCTCTGTCTACATCACCTACGTCAGCAATCAGACCTA
CCAGGAGCGGACCTATAAGCAGCTGCTCCAGGAGAAGGCAGCTTTCCGGGAGCTGATCGC
GCAGCTAGAGCTCGACCCCAAGTGCAGGGGGCTGCCCTTCTCCTCCTTCCTCATCCTGCC
TTTCCAGAGGATCACACGCCTCAAGCTGTTGGTCCAGAACATCCTGAAGAGGGTAGAAGA
GAGGTCTGAGCGGGAGTGCACTGCTTTGGATGCTCACAAGGAGCTGGAAATGGTGGTGAA
GGCATGCAACGAGGGCGTCAGGAAAATGAGCCGCACGGAACAGATGATCAGCATTCAGAA
GAAGATGGAGTTCAAGATCAAGTCGGTGCCCATCATCTCCCACTCCCGCTGGCTGCTGAA
GCAGGGTGAGCTGCAGCAGATGTCAGGCCCCAAGACCTCCCGGACCCTGAGGACCAAGAA
GCTCTTCCACGAAATTTACCTCTTCCTGTTCAACGACCTGCTGGTGATCTGCCGGCAGAT
TCCAGGAGACAAGTACCAGGTATTTGACTCAGCTCCGCGGGGACTGCTGCGTGTGGAGGA
GCTGGAGGACCAGGGCCAGACGCTGGCCAACGTGTTCATCCTGCGGCTGCTGGAGAACGC
AGATGACCGGGAGGCCACCTACATGCTAAAGGCGTCCTCTCAGAGTGAGATGAAGCGTTG
GATGACCTCACTGGCCCCCAACAGGAGGACCAAGTTTGTTTCGTTCACATCCCGGCTGCT
GGACTGCCCCCAGGTCCAGTGCGTGCACCCATACGTGGCTCAGCAGCCAGACGAGCTGAC
GCTGGAGCTCGCCGACATCCTCAACATCCTGGACAAGACTGACGACGGGTGGATCTTTGG
CGAGCGTCTGCACGACCAGGAGAGAGGCTGGTTCCCCAGCTCCATGACTGAGGAGATCTT
GAATCCCAAGATCCGGTCCCAGAACCTCAAGGAATGTTTCCGTGTCCACAAGATGGATGA
CCCTCAGCGCAGCCAGAACAAGGACCGCAGGAAGCTGGGCAGCCGGAATCGGCAAGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT