RASGEF1C ORF clone pFN21AE4092

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4092

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Clone info.

Species

human

Clone Name

pFN21AE4092 Sequence (with vector) Restriction Map

Product ID

FHC22410

ORF Sequence

DNA sequence (1398 bp)
Protein sequence (466 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4092

Symbol

RASGEF1C

Description

RasGEF domain family, member 1C

Original Clone ID

cp94092

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1398 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 466 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4452.1 5.1e-190 100.0 RASGEF1C CCDS75152.1 2.3e-125 68.0 RASGEF1B CCDS34022.1 1.3e-124 67.9 RASGEF1B CCDS75151.1 9.1e-106 62.3 RASGEF1B CCDS7202.2 2.2e-97 53.9 RASGEF1A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000651 40 129 PF00618 Ras-like guanine nucleotide exchange factor IPR001895 200 395 PF00617 Guanine-nucleotide dissociation stimulator CDC25 HMMSmart IPR001895 196 447 SM00147 Guanine-nucleotide dissociation stimulator CDC25 ProfileScan IPR000651 34 164 PS50212 Ras-like guanine nucleotide exchange factor IPR001895 200 446 PS50009 Guanine-nucleotide dissociation stimulator CDC25
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001287664 472 614532 68.0 99.8 XP_016863302 473 614532 67.9 99.8 NP_689758 473 614532 67.9 99.8 NP_001287665 431 614532 62.3 99.8 XP_016863301 432 614532 62.2 99.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4092 Download

>KIEE4092  1398 bp
ATGCCACAGACGCTGAGTGCCTCCGACATGGTCACCCCAGGCAGCCTCAGCCCACCCCCC
ACCGAGCCCACAGATGGCGAACAGGCTGGGCAGCCCCTCCTGGATGGAGCGCCATCCTCA
GCCTCCCTGGAAACACTGATCCAGCACCTGGTGCCCACAGCCGACTACTACCCCGAGAAA
GCCTACATCTTCACCTTCCTGCTGAGCTCTCGCCTCTTCATCGAGCCCCGGGAGCTCCTG
GCCCGGGTCTGCCACCTGTGCATCGAGCAGCAGCAGCTGGACAAGCCGGTGCTGGACAAG
GCCCGGGTCCGGAAGTTCGGCCCCAAACTGCTGCAGCTGTTGGCCGAGTGGACCGAGACC
TTCCCAAGGGACTTCCAGGAGGAGTCGACTATCGGGCACCTTAAGGACGTCGTGGGCCGC
ATCGCCCCCTGTGACGAGGCATACCGGAAGAGGATGCATCAGCTCCTACAGGCTCTGCAC
CAGAAGCTGGCGGCTCTGCGCCAGGGGCCAGAAGGTCTGGTGGGTGCCGACAAGCCCATC
TCCTACAGGACCAAGCCACCAGCCTCCATCCACAGGGAGCTCCTTGGTGTCTGCAGCGAC
CCCTACACACTGGCCCAGCAGCTGACCCACGTGGAACTGGAGCGGCTGCGGCACATCGGG
CCTGAGGAGTTTGTCCAGGCCTTTGTGAACAAGGACCCTCTGGCCAGCACAAAGCCCTGC
TTCAGTGACAAGACCAGCAACCTGGAGGCTTATGTGAAATGGTTCAACAGGCTGTGCTAC
CTGGTGGCAACTGAGATCTGCATGCCAGCCAAGAAGAAGCAGAGGGCCCAGGTGATTGAG
TTCTTCATCGACGTGGCCCGCGAGTGCTTCAACATCGGCAACTTCAACTCCCTCATGGCC
ATCATCTCCGGCATGAACATGAGCCCTGTCTCCAGGCTGAAGAAGACCTGGGCCAAAGTG
AGGACGGCCAAGTTTTTCATCCTCGAGCACCAGATGGACCCAACGGGGAATTTCTGCAAC
TACAGGACAGCCCTGCGCGGGGCGGCCCACCGCTCCCTGACGGCCCACAGCAGCCGAGAG
AAGATTGTCATTCCTTTCTTCAGCCTGCTCATCAAAGACATCTACTTCCTGAATGAGGGC
TGCGCCAACCGCCTTCCCAATGGACACGTCAACTTTGAGAAATTCCTGGAGCTGGCCAAG
CAGGTGGGGGAGTTCATCACCTGGAAACAAGTGGAGTGTCCCTTCGAGCAAGACGCCAGC
ATCACCCACTACCTGTACACCGCCCCCATCTTCAGTGAGGATGGTCTTTATTTGGCTTCT
TATGAAAGTGAGAGCCCAGAGAACCAAACAGAAAAAGAAAGATGGAAAGCTCTAAGATCT
TCTATTTTGGGGAAGACA

ORF Protein

Cloned ORF protein sequence for pFN21AE4092 Download

>KIEE4092  466 aa
MPQTLSASDMVTPGSLSPPPTEPTDGEQAGQPLLDGAPSSASLETLIQHLVPTADYYPEK
AYIFTFLLSSRLFIEPRELLARVCHLCIEQQQLDKPVLDKARVRKFGPKLLQLLAEWTET
FPRDFQEESTIGHLKDVVGRIAPCDEAYRKRMHQLLQALHQKLAALRQGPEGLVGADKPI
SYRTKPPASIHRELLGVCSDPYTLAQQLTHVELERLRHIGPEEFVQAFVNKDPLASTKPC
FSDKTSNLEAYVKWFNRLCYLVATEICMPAKKKQRAQVIEFFIDVARECFNIGNFNSLMA
IISGMNMSPVSRLKKTWAKVRTAKFFILEHQMDPTGNFCNYRTALRGAAHRSLTAHSSRE
KIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFLELAKQVGEFITWKQVECPFEQDAS
ITHYLYTAPIFSEDGLYLASYESESPENQTEKERWKALRSSILGKT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4092 Download

>pFN21AE4092  6103 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCACAGACGCTGAGTGCCTCCGACATGGTCAC
CCCAGGCAGCCTCAGCCCACCCCCCACCGAGCCCACAGATGGCGAACAGGCTGGGCAGCC
CCTCCTGGATGGAGCGCCATCCTCAGCCTCCCTGGAAACACTGATCCAGCACCTGGTGCC
CACAGCCGACTACTACCCCGAGAAAGCCTACATCTTCACCTTCCTGCTGAGCTCTCGCCT
CTTCATCGAGCCCCGGGAGCTCCTGGCCCGGGTCTGCCACCTGTGCATCGAGCAGCAGCA
GCTGGACAAGCCGGTGCTGGACAAGGCCCGGGTCCGGAAGTTCGGCCCCAAACTGCTGCA
GCTGTTGGCCGAGTGGACCGAGACCTTCCCAAGGGACTTCCAGGAGGAGTCGACTATCGG
GCACCTTAAGGACGTCGTGGGCCGCATCGCCCCCTGTGACGAGGCATACCGGAAGAGGAT
GCATCAGCTCCTACAGGCTCTGCACCAGAAGCTGGCGGCTCTGCGCCAGGGGCCAGAAGG
TCTGGTGGGTGCCGACAAGCCCATCTCCTACAGGACCAAGCCACCAGCCTCCATCCACAG
GGAGCTCCTTGGTGTCTGCAGCGACCCCTACACACTGGCCCAGCAGCTGACCCACGTGGA
ACTGGAGCGGCTGCGGCACATCGGGCCTGAGGAGTTTGTCCAGGCCTTTGTGAACAAGGA
CCCTCTGGCCAGCACAAAGCCCTGCTTCAGTGACAAGACCAGCAACCTGGAGGCTTATGT
GAAATGGTTCAACAGGCTGTGCTACCTGGTGGCAACTGAGATCTGCATGCCAGCCAAGAA
GAAGCAGAGGGCCCAGGTGATTGAGTTCTTCATCGACGTGGCCCGCGAGTGCTTCAACAT
CGGCAACTTCAACTCCCTCATGGCCATCATCTCCGGCATGAACATGAGCCCTGTCTCCAG
GCTGAAGAAGACCTGGGCCAAAGTGAGGACGGCCAAGTTTTTCATCCTCGAGCACCAGAT
GGACCCAACGGGGAATTTCTGCAACTACAGGACAGCCCTGCGCGGGGCGGCCCACCGCTC
CCTGACGGCCCACAGCAGCCGAGAGAAGATTGTCATTCCTTTCTTCAGCCTGCTCATCAA
AGACATCTACTTCCTGAATGAGGGCTGCGCCAACCGCCTTCCCAATGGACACGTCAACTT
TGAGAAATTCCTGGAGCTGGCCAAGCAGGTGGGGGAGTTCATCACCTGGAAACAAGTGGA
GTGTCCCTTCGAGCAAGACGCCAGCATCACCCACTACCTGTACACCGCCCCCATCTTCAG
TGAGGATGGTCTTTATTTGGCTTCTTATGAAAGTGAGAGCCCAGAGAACCAAACAGAAAA
AGAAAGATGGAAAGCTCTAAGATCTTCTATTTTGGGGAAGACAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT