HECTD2 ORF clone pFN21AE4223

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4223

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Clone info.

Species

human

Clone Name

pFN21AE4223 Sequence (with vector) Restriction Map

Product ID

FHC23938

ORF Sequence

DNA sequence (2328 bp)
Protein sequence (776 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4223

Symbol

HECTD2

Description

HECT domain containing E3 ubiquitin protein ligase 2, transcript variant 1

Original Clone ID

cp94223

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2328 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 776 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7414.1 0 99.9 HECTD2 CCDS60591.1 0 99.4 HECTD2 CCDS7415.1 1.8e-74 99.5 HECTD2 CCDS58769.1 1.9e-27 36.5 ITCH CCDS13234.1 2.2e-27 36.5 ITCH The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000569 467 775 PF00632 HECT HMMSmart IPR000569 435 776 SM00119 HECT ProfileScan IPR000569 437 776 PS50237 HECT Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4223 Download

>KIEE4223  2328 bp
ATGAGTGAGGCGGTTCGGGTACCCTCGCCCGCCACTCCGCTGGTGGTGGCGGCGGCCGCG
CCTGAGGAGAGGAAAGGGAAGGAGTCAGAGCGCGAGAAGCTGCCGCCCATCGTATCGGCG
GGCGCCGGCGCGACCGCGGGTTTGGACAGAGGAGCCAAAGGCCAAATTTCCACTTTCAGC
AGTTTTATTTCAGCTGTTAGCCCGAAGAAAGAAGCTGCTGAAAACAGAAGTTCACCTGCA
CATCTTGTTTTCCCTAACATCAAGAATGTGAGAGAACCACCACCAATTTGCCTTGATGTT
AGACAAAAACAGCGTACATCTATGGATGCATCATCATCCGAAATGAAGGCCCCAGTCCTT
CCAGAACCTATTCTTCCTATCCAGCCCAAAACTGTGAAAGACTTTCAGGAAGATGTAGAA
AAAGTTAAGTCATCAGGAGATTGGAAAGCAGTACATGATTTTTATCTAACAACGTTTGAT
TCTTTCCCAGAATTAAATGCTGCATTTAAGAAAGATGCCACTGCCTCATTTAACACCATT
GAAGACTCTGGGATTAATGCTAAATTTGTGAATGCTGTGTATGATACCTTACTTAATACT
CCTCAAGACGTTCAGAAGACAGTATTAAAGGGAATCATTAACAGCCTGTTACGAGAATGG
AAAGGTCCACGAACAAAAGATGATCTTAGAGCATATTTTATACTTTTACAGAATCCTCAG
TTTAATAACACATCTACGTATGTCATCTATGCTCACTTGCTACGACAGATAGCTACCTTA
GTGGAAGCTGACCATCATTTCCTAGTTCACTGGTTTAAAAAATTGTCCCAGAAGAGGTTC
AAACAATTGGTAGAGAGATTGCTGCAATTTATTTCTTTACGCCTGTTTCCTGCAAAGCCT
GAAGAATTTCCACCTATAACAAAGTGTTCCTGGTGGATTCCATCAGCCGCTAAAGTGTTG
GCTTTACTTAATACTGCAAACAATTTAGTTCACCCTCCCCTTATTCCTTATACTGATTTC
TATAATTCTACATTGGATCACATTGATCTCATGGAAGAATATCATACTTGGCAAAACTTT
GGAAACTCTCACAGGTTTTCCTTCTGTCAGTACCCATTCGTTATTTCTGTAGCTGCAAAA
AAAATCATTATTCAGAGAGACTCAGAGCAACAGATGATAAACATCGCAAGGCAAAGTCTG
GTGGATAAAGTATCTCGAAGACAGAGACCTGATATGAATATATTATTTCTAAATATGAAA
GTAAGACGGACACATCTGGTTAGCGATTCACTTGATGAGCTAACCCGGAAAAGAGCCGAT
TTGAAAAAGAAGTTAAAAGTTACATTTGTAGGGGAAGCTGGTTTGGATATGGGTGGTTTG
ACTAAAGAATGGTTCCTTCTTCTAATTCGCCAAATTTTTCATCCAGATTATGGCATGTTT
ACATATCACAAGGATTCACACTGCCATTGGTTTAGCAGCTTTAAATGTGATAACTATTCT
GAATTCCGATTGGTTGGAATTCTTATGGGACTAGCTGTTTATAACAGCATCACCTTGGAT
ATTCGTTTCCCTCCCTGCTGTTACAAGAAATTATTGAGCCCTCCCATCATTCCTAGTGAT
CAAAATATACCAGTAGGCATCTGCAATGTTACCGTGGACGACTTATGTCAAATTATGCCT
GAGTTGGCCCATGGATTAAGTGAACTCTTATCACATGAAGGCAATGTTGAAGAAGATTTC
TATTCAACATTTCAGGTTTTTCAAGAAGAATTTGGAATAATCAAGTCCTATAATTTAAAG
CCCGGTGGTGATAAAATTTCAGTTACCAATCAAAATAGAAAAGAATATGTACAGCTTTAT
ACTGACTTCCTTCTGAACAAATCCATCTATAAGCAGTTTGCTGCATTTTATTATGGATTT
CATAGTGTGTGTGCTTCAAATGCCCTAATGCTGCTTCGTCCAGAAGAGGTTGAAATTTTG
GTCTGTGGAAGTCCTGACCTGGATATGCATGCTCTGCAGAGAAGTACTCAGTATGATGGC
TATGCAAAAACGGACTTAACTATAAAATACTTTTGGGATGTTGTGCTTGGATTTCCTCTT
GATCTTCAAAAGAAGTTGCTACATTTTACTACAGGGAGTGACAGAGTACCTGTAGGAGGG
ATGGCTGATTTGAACTTTAAAATCTCAAAGAATGAAACTTCTACTAACTGCTTACCTGTG
GCCCATACCTGCTTCAATCAACTTTGCCTTCCCCCCTACAAGAGCAAAAAGGATCTGAAA
CAGAAATTGATCATTGGAATTTCAAATTCAGAAGGTTTTGGACTTGAG

ORF Protein

Cloned ORF protein sequence for pFN21AE4223 Download

>KIEE4223  776 aa
MSEAVRVPSPATPLVVAAAAPEERKGKESEREKLPPIVSAGAGATAGLDRGAKGQISTFS
SFISAVSPKKEAAENRSSPAHLVFPNIKNVREPPPICLDVRQKQRTSMDASSSEMKAPVL
PEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTI
EDSGINAKFVNAVYDTLLNTPQDVQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQ
FNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKLSQKRFKQLVERLLQFISLRLFPAKP
EEFPPITKCSWWIPSAAKVLALLNTANNLVHPPLIPYTDFYNSTLDHIDLMEEYHTWQNF
GNSHRFSFCQYPFVISVAAKKIIIQRDSEQQMINIARQSLVDKVSRRQRPDMNILFLNMK
VRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMF
TYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSD
QNIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLK
PGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEIL
VCGSPDLDMHALQRSTQYDGYAKTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGG
MADLNFKISKNETSTNCLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4223 Download

>pFN21AE4223  7033 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTGAGGCGGTTCGGGTACCCTCGCCCGCCAC
TCCGCTGGTGGTGGCGGCGGCCGCGCCTGAGGAGAGGAAAGGGAAGGAGTCAGAGCGCGA
GAAGCTGCCGCCCATCGTATCGGCGGGCGCCGGCGCGACCGCGGGTTTGGACAGAGGAGC
CAAAGGCCAAATTTCCACTTTCAGCAGTTTTATTTCAGCTGTTAGCCCGAAGAAAGAAGC
TGCTGAAAACAGAAGTTCACCTGCACATCTTGTTTTCCCTAACATCAAGAATGTGAGAGA
ACCACCACCAATTTGCCTTGATGTTAGACAAAAACAGCGTACATCTATGGATGCATCATC
ATCCGAAATGAAGGCCCCAGTCCTTCCAGAACCTATTCTTCCTATCCAGCCCAAAACTGT
GAAAGACTTTCAGGAAGATGTAGAAAAAGTTAAGTCATCAGGAGATTGGAAAGCAGTACA
TGATTTTTATCTAACAACGTTTGATTCTTTCCCAGAATTAAATGCTGCATTTAAGAAAGA
TGCCACTGCCTCATTTAACACCATTGAAGACTCTGGGATTAATGCTAAATTTGTGAATGC
TGTGTATGATACCTTACTTAATACTCCTCAAGACGTTCAGAAGACAGTATTAAAGGGAAT
CATTAACAGCCTGTTACGAGAATGGAAAGGTCCACGAACAAAAGATGATCTTAGAGCATA
TTTTATACTTTTACAGAATCCTCAGTTTAATAACACATCTACGTATGTCATCTATGCTCA
CTTGCTACGACAGATAGCTACCTTAGTGGAAGCTGACCATCATTTCCTAGTTCACTGGTT
TAAAAAATTGTCCCAGAAGAGGTTCAAACAATTGGTAGAGAGATTGCTGCAATTTATTTC
TTTACGCCTGTTTCCTGCAAAGCCTGAAGAATTTCCACCTATAACAAAGTGTTCCTGGTG
GATTCCATCAGCCGCTAAAGTGTTGGCTTTACTTAATACTGCAAACAATTTAGTTCACCC
TCCCCTTATTCCTTATACTGATTTCTATAATTCTACATTGGATCACATTGATCTCATGGA
AGAATATCATACTTGGCAAAACTTTGGAAACTCTCACAGGTTTTCCTTCTGTCAGTACCC
ATTCGTTATTTCTGTAGCTGCAAAAAAAATCATTATTCAGAGAGACTCAGAGCAACAGAT
GATAAACATCGCAAGGCAAAGTCTGGTGGATAAAGTATCTCGAAGACAGAGACCTGATAT
GAATATATTATTTCTAAATATGAAAGTAAGACGGACACATCTGGTTAGCGATTCACTTGA
TGAGCTAACCCGGAAAAGAGCCGATTTGAAAAAGAAGTTAAAAGTTACATTTGTAGGGGA
AGCTGGTTTGGATATGGGTGGTTTGACTAAAGAATGGTTCCTTCTTCTAATTCGCCAAAT
TTTTCATCCAGATTATGGCATGTTTACATATCACAAGGATTCACACTGCCATTGGTTTAG
CAGCTTTAAATGTGATAACTATTCTGAATTCCGATTGGTTGGAATTCTTATGGGACTAGC
TGTTTATAACAGCATCACCTTGGATATTCGTTTCCCTCCCTGCTGTTACAAGAAATTATT
GAGCCCTCCCATCATTCCTAGTGATCAAAATATACCAGTAGGCATCTGCAATGTTACCGT
GGACGACTTATGTCAAATTATGCCTGAGTTGGCCCATGGATTAAGTGAACTCTTATCACA
TGAAGGCAATGTTGAAGAAGATTTCTATTCAACATTTCAGGTTTTTCAAGAAGAATTTGG
AATAATCAAGTCCTATAATTTAAAGCCCGGTGGTGATAAAATTTCAGTTACCAATCAAAA
TAGAAAAGAATATGTACAGCTTTATACTGACTTCCTTCTGAACAAATCCATCTATAAGCA
GTTTGCTGCATTTTATTATGGATTTCATAGTGTGTGTGCTTCAAATGCCCTAATGCTGCT
TCGTCCAGAAGAGGTTGAAATTTTGGTCTGTGGAAGTCCTGACCTGGATATGCATGCTCT
GCAGAGAAGTACTCAGTATGATGGCTATGCAAAAACGGACTTAACTATAAAATACTTTTG
GGATGTTGTGCTTGGATTTCCTCTTGATCTTCAAAAGAAGTTGCTACATTTTACTACAGG
GAGTGACAGAGTACCTGTAGGAGGGATGGCTGATTTGAACTTTAAAATCTCAAAGAATGA
AACTTCTACTAACTGCTTACCTGTGGCCCATACCTGCTTCAATCAACTTTGCCTTCCCCC
CTACAAGAGCAAAAAGGATCTGAAACAGAAATTGATCATTGGAATTTCAAATTCAGAAGG
TTTTGGACTTGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT