EFHC1 ORF clone pFN21AE4546

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4546

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Clone info.

Species

human

Clone Name

pFN21AE4546 Sequence (with vector) Restriction Map

Product ID

FHC09003

ORF Sequence

DNA sequence (1920 bp)
Protein sequence (640 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4546

Symbol

EFHC1

Alias : EJM1, POC9, RIB72, dJ304B14.2

Description

EF-hand domain (C-terminal) containing 1, transcript variant A

Original Clone ID

oc94546

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1920 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 640 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4942.1 0 99.7 EFHC1 CCDS55021.1 0 99.7 EFHC1 CCDS55405.1 2.2e-38 36.6 EFHC2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR010554 122 153 PF06565 Protein of unknown function DUF1126 IPR010554 263 296 PF06565 Protein of unknown function DUF1126 IPR010554 439 471 PF06565 Protein of unknown function DUF1126 HMMSmart IPR006602 93 198 SM00676 Uncharacterised protein domain DM10 IPR006602 239 359 SM00676 Uncharacterised protein domain DM10 IPR006602 416 520 SM00676 Uncharacterised protein domain DM10 ProfileScan IPR006602 93 198 PS51336 Uncharacterised protein domain DM10 IPR006602 239 359 PS51336 Uncharacterised protein domain DM10 IPR006602 416 520 PS51336 Uncharacterised protein domain DM10 IPR018249 574 609 PS50222 EF-HAND 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060570 640 254770,607631,608815 99.7 100.0 NP_001165891 621 254770,607631,608815 99.7 96.7 NP_079460 749 300817 36.6 83.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4546 Download

>KIEE4546  1920 bp
ATGGTGTCCAATCCCGTGCATGGCTTGCCCTTTCTTCCGGGCACGTCCTTTAAGGACTCT
ACGAAAACAGCCTTCCACAGAAGTCAGACGCTGAGCTACAGGAACGGCTATGCAATTGTT
CGACGTCCAACAGTTGGGATAGGCGGAGACCGGCTCCAGTTCAACCAGCTGTCCCAGGCT
GAGCTGGATGAGTTGGCCAGTAAGGCACCAGTCTTAACTTATGGCCAACCTAAACAAGCC
CCACCTGCGGATTTTATTCCTGCGCATGTGGCCTTTGACAAAAAGGTACTGAAATTTGAT
GCCTATTTCCAAGAAGATGTTCCTATGTCAACTGAGGAACAGTATAGGATCCGTCAGGTG
AACATTTACTATTATCTAGAAGATGACAGCATGTCTGTCATAGAGCCTGTTGTAGAAAAT
TCTGGAATCCTTCAAGGCAAGTTAATAAAACGCCAGCGGCTAGCCAAGAATGACCGGGGT
GACCATTACCATTGGAAAGACCTAAATCGAGGAATAAACATCACAATTTATGGCAAAACT
TTCCGCGTTGTTGACTGTGACCAATTCACACAGGTATTTTTAGAAAGCCAAGGAATTGAG
TTAAATCCACCAGAGAAGATGGCTCTTGATCCTTACACTGAACTCCGAAAACAGCCTCTT
CGTAAGTATGTCACCCCATCAGACTTTGATCAACTCAAGCAATTTCTCACCTTTGACAAA
CAGGTCCTTCGATTCTATGCAATCTGGGATGATACAGACAGCATGTATGGTGAATGTCGG
ACCTACATCATTCATTACTATCTTATGGATGATACGGTGGAAATTCGAGAGGTCCACGAA
CGGAATGATGGGATAGATCCTTTCCCACTCCTAATGAACCGCCAGCGTGTGCCCAAAGTT
TTGGTGGAAAATGCAAAGAACTTCCCTCAGTGTGTGCTAGAAATCTCTGACCAAGAAGTG
TTGGAATGGTATACTGCTAAAGACTTCATTGTTGGGAAGTCACTCACTATCCTTGGGAGA
ACTTTCTTCATTTATGATTGTGATCCATTTACTCGACGGTATTACAAAGAGAAGTTTGGA
ATCACTGATTTACCACGTATTGATGTGAGCAAGCGGGAACCACCTCCAGTAAAACAGGAG
TTGCCTCCTTATAACGGTTTTGGACTAGTGGAAGATTCTGCTCAGAATTGTTTTGCTCTC
ATTCCAAAAGCTCCAAAAAAAGACGTTATTAAAATGCTGGTGAATGATAACAAGGTGCTT
CGTTATTTGGCTGTACTGGAATCCCCCATCCCAGAAGACAAAGACCGCAGATTTGTCTTC
TCTTACTTTCTAGCTACCGACATGATCAGTATCTTTGAGCCTCCTGTTCGCAATTCTGGT
ATCATTGGGGGCAAGTACCTTGGCAGGACTAAAGTTGTTAAACCATACTCTACAGTGGAC
AACCCTGTCTACTATGGCCCCAGTGACTTCTTCATTGGTGCTGTGATTGAAGTGTTTGGT
CACCGGTTCATCATCCTTGATACAGACGAGTATGTTTTGAAATACATGGAGAGCAACGCT
GCCCAGTATTCACCAGAAGCACTCGCGTCAATTCAGAACCATGTCCGAAAGCGAGAAGCG
CCTGCTCCAGAAGCAGAAAGCAAGCAAACTGAAAAGGATCCAGGCGTGCAGGAATTGGAA
GCATTAATAGACACAATTCAGAAGCAACTGAAAGATCACTCATGCAAAGACAACATTCGT
GAGGCATTTCAAATTTATGACAAGGAAGCTTCAGGATATGTGGACAGAGACATGTTCTTT
AAAATCTGTGAATCGCTTAACGTCCCAGTGGATGACTCCTTGGTTAAGGAGTTACTCAGG
ATGTGCTCTCATGGAGAAGGCAAAATTAACTACTATAACTTTGTTCGTGCTTTCTCAAAC

ORF Protein

Cloned ORF protein sequence for pFN21AE4546 Download

>KIEE4546  640 aa
MVSNPVHGLPFLPGTSFKDSTKTAFHRSQTLSYRNGYAIVRRPTVGIGGDRLQFNQLSQA
ELDELASKAPVLTYGQPKQAPPADFIPAHVAFDKKVLKFDAYFQEDVPMSTEEQYRIRQV
NIYYYLEDDSMSVIEPVVENSGILQGKLIKRQRLAKNDRGDHYHWKDLNRGINITIYGKT
FRVVDCDQFTQVFLESQGIELNPPEKMALDPYTELRKQPLRKYVTPSDFDQLKQFLTFDK
QVLRFYAIWDDTDSMYGECRTYIIHYYLMDDTVEIREVHERNDGIDPFPLLMNRQRVPKV
LVENAKNFPQCVLEISDQEVLEWYTAKDFIVGKSLTILGRTFFIYDCDPFTRRYYKEKFG
ITDLPRIDVSKREPPPVKQELPPYNGFGLVEDSAQNCFALIPKAPKKDVIKMLVNDNKVL
RYLAVLESPIPEDKDRRFVFSYFLATDMISIFEPPVRNSGIIGGKYLGRTKVVKPYSTVD
NPVYYGPSDFFIGAVIEVFGHRFIILDTDEYVLKYMESNAAQYSPEALASIQNHVRKREA
PAPEAESKQTEKDPGVQELEALIDTIQKQLKDHSCKDNIREAFQIYDKEASGYVDRDMFF
KICESLNVPVDDSLVKELLRMCSHGEGKINYYNFVRAFSN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4546 Download

>pFN21AE4546  6625 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTGTCCAATCCCGTGCATGGCTTGCCCTTTCT
TCCGGGCACGTCCTTTAAGGACTCTACGAAAACAGCCTTCCACAGAAGTCAGACGCTGAG
CTACAGGAACGGCTATGCAATTGTTCGACGTCCAACAGTTGGGATAGGCGGAGACCGGCT
CCAGTTCAACCAGCTGTCCCAGGCTGAGCTGGATGAGTTGGCCAGTAAGGCACCAGTCTT
AACTTATGGCCAACCTAAACAAGCCCCACCTGCGGATTTTATTCCTGCGCATGTGGCCTT
TGACAAAAAGGTACTGAAATTTGATGCCTATTTCCAAGAAGATGTTCCTATGTCAACTGA
GGAACAGTATAGGATCCGTCAGGTGAACATTTACTATTATCTAGAAGATGACAGCATGTC
TGTCATAGAGCCTGTTGTAGAAAATTCTGGAATCCTTCAAGGCAAGTTAATAAAACGCCA
GCGGCTAGCCAAGAATGACCGGGGTGACCATTACCATTGGAAAGACCTAAATCGAGGAAT
AAACATCACAATTTATGGCAAAACTTTCCGCGTTGTTGACTGTGACCAATTCACACAGGT
ATTTTTAGAAAGCCAAGGAATTGAGTTAAATCCACCAGAGAAGATGGCTCTTGATCCTTA
CACTGAACTCCGAAAACAGCCTCTTCGTAAGTATGTCACCCCATCAGACTTTGATCAACT
CAAGCAATTTCTCACCTTTGACAAACAGGTCCTTCGATTCTATGCAATCTGGGATGATAC
AGACAGCATGTATGGTGAATGTCGGACCTACATCATTCATTACTATCTTATGGATGATAC
GGTGGAAATTCGAGAGGTCCACGAACGGAATGATGGGATAGATCCTTTCCCACTCCTAAT
GAACCGCCAGCGTGTGCCCAAAGTTTTGGTGGAAAATGCAAAGAACTTCCCTCAGTGTGT
GCTAGAAATCTCTGACCAAGAAGTGTTGGAATGGTATACTGCTAAAGACTTCATTGTTGG
GAAGTCACTCACTATCCTTGGGAGAACTTTCTTCATTTATGATTGTGATCCATTTACTCG
ACGGTATTACAAAGAGAAGTTTGGAATCACTGATTTACCACGTATTGATGTGAGCAAGCG
GGAACCACCTCCAGTAAAACAGGAGTTGCCTCCTTATAACGGTTTTGGACTAGTGGAAGA
TTCTGCTCAGAATTGTTTTGCTCTCATTCCAAAAGCTCCAAAAAAAGACGTTATTAAAAT
GCTGGTGAATGATAACAAGGTGCTTCGTTATTTGGCTGTACTGGAATCCCCCATCCCAGA
AGACAAAGACCGCAGATTTGTCTTCTCTTACTTTCTAGCTACCGACATGATCAGTATCTT
TGAGCCTCCTGTTCGCAATTCTGGTATCATTGGGGGCAAGTACCTTGGCAGGACTAAAGT
TGTTAAACCATACTCTACAGTGGACAACCCTGTCTACTATGGCCCCAGTGACTTCTTCAT
TGGTGCTGTGATTGAAGTGTTTGGTCACCGGTTCATCATCCTTGATACAGACGAGTATGT
TTTGAAATACATGGAGAGCAACGCTGCCCAGTATTCACCAGAAGCACTCGCGTCAATTCA
GAACCATGTCCGAAAGCGAGAAGCGCCTGCTCCAGAAGCAGAAAGCAAGCAAACTGAAAA
GGATCCAGGCGTGCAGGAATTGGAAGCATTAATAGACACAATTCAGAAGCAACTGAAAGA
TCACTCATGCAAAGACAACATTCGTGAGGCATTTCAAATTTATGACAAGGAAGCTTCAGG
ATATGTGGACAGAGACATGTTCTTTAAAATCTGTGAATCGCTTAACGTCCCAGTGGATGA
CTCCTTGGTTAAGGAGTTACTCAGGATGTGCTCTCATGGAGAAGGCAAAATTAACTACTA
TAACTTTGTTCGTGCTTTCTCAAACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT