CYP8B1 ORF clone pFN21AE5692

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5692

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Clone info.

Species

human

Clone Name

pFN21AE5692 Sequence (with vector) Restriction Map

Product ID

FHC16546

ORF Sequence

DNA sequence (1503 bp)
Protein sequence (501 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5692

Symbol

CYP8B1

Alias : CP8B, CYP12

Description

cytochrome P450, family 8, subfamily B, polypeptide 1

Original Clone ID

cp95692

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1503 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 501 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2707.1 0 99.8 CYP8B1 CCDS13419.1 5.1e-83 40.9 PTGIS CCDS6171.1 2.7e-68 35.5 CYP7A1 CCDS6180.1 1e-62 36.1 CYP7B1 CCDS4916.1 6.1e-17 26.5 CYP39A1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002403 33 50 PR00465 Cytochrome P450 IPR002403 55 78 PR00465 Cytochrome P450 IPR002403 273 299 PR00465 Cytochrome P450 IPR002403 337 353 PR00465 Cytochrome P450 IPR002403 374 388 PR00465 Cytochrome P450 IPR002403 391 409 PR00465 Cytochrome P450 IPR002403 424 440 PR00465 Cytochrome P450 IPR002403 440 458 PR00465 Cytochrome P450 HMMPfam IPR001128 34 490 PF00067 Cytochrome P450
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 3 LWGPVLGALLVVIAGYLCLPGML 25 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004382 501 602172 99.8 100.0 NP_000952 500 145500,601699 40.9 99.0 NP_000771 504 118455 35.5 99.0 NP_004811 506 270800,603711,613812 36.1 98.8 XP_016869491 528 270800,603711,613812 35.9 95.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5692 Download

>KIEE5692  1503 bp
ATGGTTCTCTGGGGTCCAGTGCTGGGAGCTCTGCTGGTGGTCATTGCTGGATACCTGTGC
CTGCCAGGGATGCTCCGACAACGCAGGCCATGGGAGCCCCCTCTGGACAAGGGTACCGTG
CCCTGGCTTGGCCATGCCATGGCTTTCCGGAAGAATATGTTTGAATTTCTGAAGCGCATG
AGGACCAAGCATGGGGATGTGTTCACAGTGCAGCTAGGGGGCCAGTACTTCACCTTCGTC
ATGGACCCCCTCTCCTTTGGCCCCATCCTCAAGGACACACAGAGAAAACTAGACTTTGGG
CAATATGCAAAAAAACTGGTGCTGAAGGTATTTGGATACCGTTCAGTGCAAGGGGACCAT
GAGATGATACACTCAGCCAGCACCAAGCATCTGAGGGGGGATGGCTTGAAGGATCTTAAT
GAGACCATGCTGGACAGCCTGTCCTTTGTAATGCTGACGTCCAAAGGCTGGAGTCTGGAT
GCCAGTTGCTGGCATGAGGACAGCCTCTTTCGCTTCTGCTATTACATCTTGTTCACAGCT
GGCTACCTGAGCTTGTTCGGCTACACGAAGGACAAGGAGCAGGACCTGCTACAGGCAGGA
GAGTTATTCATGGAGTTCCGCAAGTTTGACCTTCTTTTCCCAAGGTTTGTCTACTCCCTG
CTGTGGCCCCGGGAGTGGCTAGAAGTGGGCCGACTCCAGCGTCTCTTTCACAAGATGCTC
TCCGTGAGCCACAGCCAGGAGAAGGAGGGCATCAGCAACTGGCTGGGCAACATGCTTCAG
TTTCTGAGGGAGCAGGGGGTACCCTCAGCTATGCAGGACAAGTTCAACTTCATGATGCTC
TGGGCCTCCCAGGGGAACACGGGGCCTACCTCTTTCTGGGCCCTCTTGTACCTCCTGAAG
CACCCAGAAGCTATTCGGGCTGTGAGGGAGGAAGCTACCCAGGTCCTGGGTGAGGCCAGG
CTGGAGACCAAGCAGTCCTTTGCCTTCAAACTCGGTGCCCTGCAACACACCCCAGTTCTA
GACAGCGTGGTGGAGGAGACGCTGCGGCTGAGGGCTGCACCCACCCTCCTCAGGTTGGTT
CATGAAGACTATACCCTGAAGATGTCCAGTGGGCAGGAGTATCTGTTCCGCCATGGAGAC
ATCCTGGCCCTCTTTCCCTACCTCTCAGTGCACATGGACCCTGACATCCACCCTGAGCCC
ACCGTCTTCAAGTACGATCGCTTCCTCAACCCTAATGGCAGCCGGAAAGTGGACTTCTTC
AAGACAGGCAAGAAGATCCACCACTACACCATGCCCTGGGGTTCGGGCGTTTCCATCTGC
CCTGGGAGGTTCTTTGCACTCAGTGAGGTGAAGCTCTTTATCCTGCTTATGGTCACACAC
TTTGACTTAGAGTTGGTGGACCCTGACACACCACTACCCCATGTTGACCCGCAGCGCTGG
GGTTTTGGCACCATGCAGCCCAGCCACGATGTGCGCTTCCGCTACCGCCTGCATCCTACA
GAG

ORF Protein

Cloned ORF protein sequence for pFN21AE5692 Download

>KIEE5692  501 aa
MVLWGPVLGALLVVIAGYLCLPGMLRQRRPWEPPLDKGTVPWLGHAMAFRKNMFEFLKRM
RTKHGDVFTVQLGGQYFTFVMDPLSFGPILKDTQRKLDFGQYAKKLVLKVFGYRSVQGDH
EMIHSASTKHLRGDGLKDLNETMLDSLSFVMLTSKGWSLDASCWHEDSLFRFCYYILFTA
GYLSLFGYTKDKEQDLLQAGELFMEFRKFDLLFPRFVYSLLWPREWLEVGRLQRLFHKML
SVSHSQEKEGISNWLGNMLQFLREQGVPSAMQDKFNFMMLWASQGNTGPTSFWALLYLLK
HPEAIRAVREEATQVLGEARLETKQSFAFKLGALQHTPVLDSVVEETLRLRAAPTLLRLV
HEDYTLKMSSGQEYLFRHGDILALFPYLSVHMDPDIHPEPTVFKYDRFLNPNGSRKVDFF
KTGKKIHHYTMPWGSGVSICPGRFFALSEVKLFILLMVTHFDLELVDPDTPLPHVDPQRW
GFGTMQPSHDVRFRYRLHPTE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5692 Download

>pFN21AE5692  6208 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTTCTCTGGGGTCCAGTGCTGGGAGCTCTGCT
GGTGGTCATTGCTGGATACCTGTGCCTGCCAGGGATGCTCCGACAACGCAGGCCATGGGA
GCCCCCTCTGGACAAGGGTACCGTGCCCTGGCTTGGCCATGCCATGGCTTTCCGGAAGAA
TATGTTTGAATTTCTGAAGCGCATGAGGACCAAGCATGGGGATGTGTTCACAGTGCAGCT
AGGGGGCCAGTACTTCACCTTCGTCATGGACCCCCTCTCCTTTGGCCCCATCCTCAAGGA
CACACAGAGAAAACTAGACTTTGGGCAATATGCAAAAAAACTGGTGCTGAAGGTATTTGG
ATACCGTTCAGTGCAAGGGGACCATGAGATGATACACTCAGCCAGCACCAAGCATCTGAG
GGGGGATGGCTTGAAGGATCTTAATGAGACCATGCTGGACAGCCTGTCCTTTGTAATGCT
GACGTCCAAAGGCTGGAGTCTGGATGCCAGTTGCTGGCATGAGGACAGCCTCTTTCGCTT
CTGCTATTACATCTTGTTCACAGCTGGCTACCTGAGCTTGTTCGGCTACACGAAGGACAA
GGAGCAGGACCTGCTACAGGCAGGAGAGTTATTCATGGAGTTCCGCAAGTTTGACCTTCT
TTTCCCAAGGTTTGTCTACTCCCTGCTGTGGCCCCGGGAGTGGCTAGAAGTGGGCCGACT
CCAGCGTCTCTTTCACAAGATGCTCTCCGTGAGCCACAGCCAGGAGAAGGAGGGCATCAG
CAACTGGCTGGGCAACATGCTTCAGTTTCTGAGGGAGCAGGGGGTACCCTCAGCTATGCA
GGACAAGTTCAACTTCATGATGCTCTGGGCCTCCCAGGGGAACACGGGGCCTACCTCTTT
CTGGGCCCTCTTGTACCTCCTGAAGCACCCAGAAGCTATTCGGGCTGTGAGGGAGGAAGC
TACCCAGGTCCTGGGTGAGGCCAGGCTGGAGACCAAGCAGTCCTTTGCCTTCAAACTCGG
TGCCCTGCAACACACCCCAGTTCTAGACAGCGTGGTGGAGGAGACGCTGCGGCTGAGGGC
TGCACCCACCCTCCTCAGGTTGGTTCATGAAGACTATACCCTGAAGATGTCCAGTGGGCA
GGAGTATCTGTTCCGCCATGGAGACATCCTGGCCCTCTTTCCCTACCTCTCAGTGCACAT
GGACCCTGACATCCACCCTGAGCCCACCGTCTTCAAGTACGATCGCTTCCTCAACCCTAA
TGGCAGCCGGAAAGTGGACTTCTTCAAGACAGGCAAGAAGATCCACCACTACACCATGCC
CTGGGGTTCGGGCGTTTCCATCTGCCCTGGGAGGTTCTTTGCACTCAGTGAGGTGAAGCT
CTTTATCCTGCTTATGGTCACACACTTTGACTTAGAGTTGGTGGACCCTGACACACCACT
ACCCCATGTTGACCCGCAGCGCTGGGGTTTTGGCACCATGCAGCCCAGCCACGATGTGCG
CTTCCGCTACCGCCTGCATCCTACAGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT