NGLY1 ORF clone pFN21AE5707

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5707

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Clone info.

Species

human

Clone Name

pFN21AE5707 Sequence (with vector) Restriction Map

Product ID

FHC16547

ORF Sequence

DNA sequence (1962 bp)
Protein sequence (654 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5707

Symbol

NGLY1

Alias : CDDG, CDG1V, PNG-1, PNG1, PNGase

Description

N-glycanase 1, transcript variant 1

Original Clone ID

oc95707

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1962 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 654 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33719.1 0 100.0 NGLY1 CCDS46777.1 0 99.8 NGLY1 CCDS46779.1 2.7e-210 99.8 NGLY1 CCDS46778.1 4.4e-127 93.7 NGLY1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018997 24 103 PF09409 PUB domain IPR002931 269 354 PF01841 Transglutaminase-like IPR006588 497 556 PF04721 Peptide N glycanase HMMSmart IPR006567 30 91 SM00580 PUG domain IPR002931 301 356 SM00460 Transglutaminase-like IPR006588 487 579 SM00613 Peptide N glycanase ProfileScan IPR006588 454 654 PS51398 Peptide N glycanase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060767 654 610661,615273 100.0 100.0 NP_001138766 612 610661,615273 99.8 93.6 XP_011532246 577 610661,615273 100.0 88.2 NP_001138767 558 610661,615273 99.8 82.3 XP_016862328 591 610661,615273 96.3 86.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5707 Download

>KIEE5707  1962 bp
ATGGCGGCGGCCGCATTGGGCAGCTCCTCAGGCTCGGCGTCCCCGGCCGTGGCTGAGCTC
TGCCAGAACACCCCGGAGACCTTTTTGGAGGCCTCCAAGCTGCTGCTCACCTATGCTGAC
AACATCCTCAGAAACCCTAATGATGAAAAATATAGATCCATCCGGATTGGAAACACAGCC
TTTTCTACTAGACTCTTGCCTGTCAGAGGAGCTGTTGAATGTTTATTTGAAATGGGCTTT
GAAGAGGGAGAAACACATCTCATCTTTCCTAAAAAAGCTTCAGTGGAGCAGCTGCAAAAA
ATTCGTGACCTGATTGCCATAGAGAGAAGTAGCAGACTGGATGGCTCAAATAAGAGCCAC
AAAGTAAAGTCATCTCAGCAACCTGCAGCCAGTACCCAGCTTCCTACAACACCATCTTCA
AATCCCAGTGGGTTAAACCAGCACACAAGGAACCGTCAAGGGCAGTCATCAGATCCACCA
TCTGCTTCAACGGTTGCTGCTGACTCAGCCATTCTAGAAGTTCTTCAGTCCAACATTCAG
CATGTGCTGGTCTATGAAAATCCTGCTCTTCAGGAGAAAGCGTTGGCTTGTATTCCGGTC
CAAGAACTAAAAAGGAAATCACAAGAAAAGTTATCGAGAGCTAGAAAATTGGATAAAGGT
ATCAATATAAGTGATGAGGATTTTCTTTTGCTGGAGCTTTTGCACTGGTTTAAGGAAGAA
TTTTTTCACTGGGTGAATAACGTTTTGTGCAGCAAATGTGGTGGACAGACTAGGTCTAGA
GATAGATCATTACTGCCCAGTGATGATGAGCTGAAGTGGGGTGCAAAGGAAGTGGAAGAT
CATTACTGTGATGCCTGCCAGTTCAGCAATCGATTCCCAAGATATAATAACCCTGAGAAA
CTTTTGGAAACAAGATGTGGACGGTGTGGCGAGTGGGCCAATTGTTTTACACTGTGCTGC
CGAGCTGTAGGGTTTGAAGCTCGCTATGTTTGGGATTACACAGACCATGTCTGGACAGAA
GTCTATTCTCCTTCTCAGCAGCGGTGGCTGCACTGTGATGCATGTGAAGATGTCTGTGAC
AAGCCACTCCTTTATGAAATAGGATGGGGCAAGAAGCTTTCCTATGTCATAGCATTTTCA
AAAGATGAGGTAGTTGATGTCACTTGGCGATATTCCTGCAAACATGAAGAGGTGATTGCC
AGAAGAACTAAGGTTAAAGAAGCATTACTTCGAGACACTATTAATGGGCTTAATAAGCAG
AGGCAACTGTTTTTGTCAGAAAACAGAAGGAAAGAACTTCTCCAGAGGATAATTGTGGAG
CTTGTTGAATTTATATCTCCCAAAACCCCTAAACCTGGAGAACTTGGGGGAAGAATATCT
GGGTCAGTGGCTTGGAGAGTAGCCCGAGGTGAAATGGGTCTACAGAGAAAAGAAACCTTG
TTTATTCCCTGTGAAAATGAGAAGATTTCTAAACAGCTCCACCTTTGTTACAATATTGTG
AAAGATCGTTATGTTCGAGTTTCAAATAACAATCAAACCATTTCTGGATGGGAGAATGGC
GTGTGGAAAATGGAATCTATATTCAGAAAAGTTGAAACAGACTGGCACATGGTATATTTG
GCCCGAAAGGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAGTGTGGGTCAGTT
GGCCTAAAAGTAGATAGCATTTCTATTAGAACAAGTAGTCAAACTTTTCAGACTGGAACA
GTAGAATGGAAATTGCGATCTGATACAGCACAAGTAGAACTGACAGGCGATAACAGTCTT
CACTCCTATGCTGATTTTTCTGGTGCCACTGAAGTTATTTTGGAAGCAGAATTAAGCAGA
GGAGATGGTGATGTCGCTTGGCAACACACCCAGCTGTTTAGACAAAGCTTAAATGACCAT
GAAGAAAATTGTTTGGAGATAATTATAAAATTCAGTGACCTT

ORF Protein

Cloned ORF protein sequence for pFN21AE5707 Download

>KIEE5707  654 aa
MAAAALGSSSGSASPAVAELCQNTPETFLEASKLLLTYADNILRNPNDEKYRSIRIGNTA
FSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSNKSH
KVKSSQQPAASTQLPTTPSSNPSGLNQHTRNRQGQSSDPPSASTVAADSAILEVLQSNIQ
HVLVYENPALQEKALACIPVQELKRKSQEKLSRARKLDKGINISDEDFLLLELLHWFKEE
FFHWVNNVLCSKCGGQTRSRDRSLLPSDDELKWGAKEVEDHYCDACQFSNRFPRYNNPEK
LLETRCGRCGEWANCFTLCCRAVGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCD
KPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYSCKHEEVIARRTKVKEALLRDTINGLNKQ
RQLFLSENRRKELLQRIIVELVEFISPKTPKPGELGGRISGSVAWRVARGEMGLQRKETL
FIPCENEKISKQLHLCYNIVKDRYVRVSNNNQTISGWENGVWKMESIFRKVETDWHMVYL
ARKEGSSFAYISWKFECGSVGLKVDSISIRTSSQTFQTGTVEWKLRSDTAQVELTGDNSL
HSYADFSGATEVILEAELSRGDGDVAWQHTQLFRQSLNDHEENCLEIIIKFSDL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5707 Download

>pFN21AE5707  6667 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCGGCCGCATTGGGCAGCTCCTCAGGCTC
GGCGTCCCCGGCCGTGGCTGAGCTCTGCCAGAACACCCCGGAGACCTTTTTGGAGGCCTC
CAAGCTGCTGCTCACCTATGCTGACAACATCCTCAGAAACCCTAATGATGAAAAATATAG
ATCCATCCGGATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGAGGAGCTGT
TGAATGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACATCTCATCTTTCCTAAAAA
AGCTTCAGTGGAGCAGCTGCAAAAAATTCGTGACCTGATTGCCATAGAGAGAAGTAGCAG
ACTGGATGGCTCAAATAAGAGCCACAAAGTAAAGTCATCTCAGCAACCTGCAGCCAGTAC
CCAGCTTCCTACAACACCATCTTCAAATCCCAGTGGGTTAAACCAGCACACAAGGAACCG
TCAAGGGCAGTCATCAGATCCACCATCTGCTTCAACGGTTGCTGCTGACTCAGCCATTCT
AGAAGTTCTTCAGTCCAACATTCAGCATGTGCTGGTCTATGAAAATCCTGCTCTTCAGGA
GAAAGCGTTGGCTTGTATTCCGGTCCAAGAACTAAAAAGGAAATCACAAGAAAAGTTATC
GAGAGCTAGAAAATTGGATAAAGGTATCAATATAAGTGATGAGGATTTTCTTTTGCTGGA
GCTTTTGCACTGGTTTAAGGAAGAATTTTTTCACTGGGTGAATAACGTTTTGTGCAGCAA
ATGTGGTGGACAGACTAGGTCTAGAGATAGATCATTACTGCCCAGTGATGATGAGCTGAA
GTGGGGTGCAAAGGAAGTGGAAGATCATTACTGTGATGCCTGCCAGTTCAGCAATCGATT
CCCAAGATATAATAACCCTGAGAAACTTTTGGAAACAAGATGTGGACGGTGTGGCGAGTG
GGCCAATTGTTTTACACTGTGCTGCCGAGCTGTAGGGTTTGAAGCTCGCTATGTTTGGGA
TTACACAGACCATGTCTGGACAGAAGTCTATTCTCCTTCTCAGCAGCGGTGGCTGCACTG
TGATGCATGTGAAGATGTCTGTGACAAGCCACTCCTTTATGAAATAGGATGGGGCAAGAA
GCTTTCCTATGTCATAGCATTTTCAAAAGATGAGGTAGTTGATGTCACTTGGCGATATTC
CTGCAAACATGAAGAGGTGATTGCCAGAAGAACTAAGGTTAAAGAAGCATTACTTCGAGA
CACTATTAATGGGCTTAATAAGCAGAGGCAACTGTTTTTGTCAGAAAACAGAAGGAAAGA
ACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAATTTATATCTCCCAAAACCCCTAAACC
TGGAGAACTTGGGGGAAGAATATCTGGGTCAGTGGCTTGGAGAGTAGCCCGAGGTGAAAT
GGGTCTACAGAGAAAAGAAACCTTGTTTATTCCCTGTGAAAATGAGAAGATTTCTAAACA
GCTCCACCTTTGTTACAATATTGTGAAAGATCGTTATGTTCGAGTTTCAAATAACAATCA
AACCATTTCTGGATGGGAGAATGGCGTGTGGAAAATGGAATCTATATTCAGAAAAGTTGA
AACAGACTGGCACATGGTATATTTGGCCCGAAAGGAAGGATCATCTTTTGCTTATATTTC
CTGGAAGTTTGAGTGTGGGTCAGTTGGCCTAAAAGTAGATAGCATTTCTATTAGAACAAG
TAGTCAAACTTTTCAGACTGGAACAGTAGAATGGAAATTGCGATCTGATACAGCACAAGT
AGAACTGACAGGCGATAACAGTCTTCACTCCTATGCTGATTTTTCTGGTGCCACTGAAGT
TATTTTGGAAGCAGAATTAAGCAGAGGAGATGGTGATGTCGCTTGGCAACACACCCAGCT
GTTTAGACAAAGCTTAAATGACCATGAAGAAAATTGTTTGGAGATAATTATAAAATTCAG
TGACCTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT