EYA4 ORF clone pFN21AE5720

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5720

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Clone info.

Species

human

Clone Name

pFN21AE5720 Sequence (with vector) Restriction Map

Product ID

FHC22767

ORF Sequence

DNA sequence (1848 bp)
Protein sequence (616 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5720

Symbol

EYA4

Alias : CMD1J, DFNA10

Description

EYA transcriptional coactivator and phosphatase 4, transcript variant 2

Original Clone ID

oc95720

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1848 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 616 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS43506.1 1.9e-159 100.0 EYA4 CCDS5165.1 9.1e-143 96.2 EYA4 CCDS75521.1 7.2e-142 95.3 EYA4 CCDS5166.1 2.8e-140 94.5 EYA4 CCDS75523.1 3.1e-133 93.2 EYA4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR005834 347 591 PF00702 Haloacid dehalogenase-like hydrolase HMMTigr IPR006545 346 615 TIGR01658 EYA
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_742101 616 601316,603550,605362 100.0 100.0 XP_005266910 616 601316,603550,605362 98.2 100.0 XP_016865859 631 601316,603550,605362 99.0 95.8 XP_016865860 626 601316,603550,605362 98.2 95.8 NP_004091 639 601316,603550,605362 96.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5720 Download

>KIEE5720  1848 bp
ATGGAAGACTCCCAGGATTTAAATGAACAATCAGTAAAGAAAACGTGCACAGAATCAGAT
GTTTCACAATCTCAGAATTCCAGGTCTATGGAAATGCAGGACCTAGCAAGTCCTCATACT
CTTGTTGGAGGTGGTGATACTCCAGGTAGCTCCAAACTGGAAAAATCTAATCTCAGCAGC
ACATCAGTTACTACAAATGGGACAGGAGTGTCTCTTCTTGCAGTCAAAACAGAGCCCTTG
AACAGCAGTGAAACCACAGCCACGACTGGAGATGGAGCGCTTGACACTTTTACTGGGTCA
GTAATTACAAGTAGTGGCTACAGCCCCAGATCAGCACATCAGTATTCCCCACAGCTGTAT
CCTTCCAAGCCCTATCCACACATTCTTTCTACACCAGCAGCTCAAACAATGTCTGCCTAT
GCAGGCCAGACTCAGTATTCGGGGATGCAGCAGCCAGCCGTCTACACAGCCTACTCACAG
ACAGGACAGCCCTACAGCTTGCCCACTTACGATTTGGGTGTGATGTTGCCAGCCATCAAG
ACAGAGAGTGGACTTTCCCAAACTCAGTCCCCATTACAGAGTGGCTGCCTCAGTTACAGC
CCAGGGTTCTCTACCCCACAGCCAGGCCAGACACCTTATTCTTACCAAATGCCAGGTTCT
AGTTTTGCACCATCATCTACTATTTATGCAAATAATTCAGTTTCCAATTCAACAAATTTC
AGTGGTTCACAACAGGATTATCCATCCTATACAGCCTTTGGCCAAAACCAGTATGCACAG
TATTATTCAGCATCAACGTATGGAGCGTATATGACATCGAATAACACAGCCGATGGCACA
CCCTCTTCAACCTCTACTTATCAGTTGCAGGAATCTCTCCCAGGACTGACTAACCAACCA
GGAGAGTTCGATACCATGCAGAGTCCCTCCACACCCATCAAAGATCTTGATGAGAGAACC
TGTAGGAGTTCTGGGTCAAAGTCCAGAGGAAGAGGCCGGAAAAATAATCCCTCCCCGCCT
CCTGATAGTGACCTGGAGCGTGTGTTTGTCTGGGATTTGGATGAAACCATCATTGTTTTT
CACTCACTGCTCACCGGGTCTTATGCACAGAAGTATGGCAAGGATCCCCCCATGGCTGTA
ACCCTTGGACTCCGCATGGAAGAAATGATTTTTAATCTTGCTGATACTCATTTGTTTTTT
AATGATTTAGAGGAGTGTGATCAAGTTCATATAGATGATGTTTCCTCTGATGATAATGGG
CAGGACTTAAGTACCTACAGTTTTGCAACTGATGGCTTCCATGCAGCTGCAAGTAGTGCA
AACCTTTGTTTGCCAACAGGTGTAAGAGGAGGGGTTGACTGGATGAGGAAGTTGGCTTTT
CGTTACAGAAGAGTAAAAGAATTATATAACACCTACAAGAACAACGTTGGAGGACTCCTT
GGCCCTGCCAAGAGGGATGCCTGGCTACAGTTAAGGGCAGAGATTGAAGGTCTGACAGAT
TCCTGGCTAACAAATGCACTTAAGTCTTTATCAATTATTAGCACTAGGAGTAACTGCATA
AATGTCTTGGTAACGACAACTCAACTGATCCCAGCACTTGCGAAGGTTCTACTCTATAGT
TTAGGAGGTGCTTTCCCCATTGAGAATATTTACAGTGCAACTAAAATAGGAAAAGAAAGT
TGCTTTGAACGAATAATGCAAAGGTTTGGCAGAAAAGTAGTGTATGTTGTAATTGGGGAT
GGTGTAGAAGAAGAACAGGCAGCAAAAAAGCACAACATGCCCTTCTGGAGGATATCCAGT
CACTCAGACCTCCTGGCTCTCCACCAAGCACTGGAATTAGAGTATTTG

ORF Protein

Cloned ORF protein sequence for pFN21AE5720 Download

>KIEE5720  616 aa
MEDSQDLNEQSVKKTCTESDVSQSQNSRSMEMQDLASPHTLVGGGDTPGSSKLEKSNLSS
TSVTTNGTGVSLLAVKTEPLNSSETTATTGDGALDTFTGSVITSSGYSPRSAHQYSPQLY
PSKPYPHILSTPAAQTMSAYAGQTQYSGMQQPAVYTAYSQTGQPYSLPTYDLGVMLPAIK
TESGLSQTQSPLQSGCLSYSPGFSTPQPGQTPYSYQMPGSSFAPSSTIYANNSVSNSTNF
SGSQQDYPSYTAFGQNQYAQYYSASTYGAYMTSNNTADGTPSSTSTYQLQESLPGLTNQP
GEFDTMQSPSTPIKDLDERTCRSSGSKSRGRGRKNNPSPPPDSDLERVFVWDLDETIIVF
HSLLTGSYAQKYGKDPPMAVTLGLRMEEMIFNLADTHLFFNDLEECDQVHIDDVSSDDNG
QDLSTYSFATDGFHAAASSANLCLPTGVRGGVDWMRKLAFRYRRVKELYNTYKNNVGGLL
GPAKRDAWLQLRAEIEGLTDSWLTNALKSLSIISTRSNCINVLVTTTQLIPALAKVLLYS
LGGAFPIENIYSATKIGKESCFERIMQRFGRKVVYVVIGDGVEEEQAAKKHNMPFWRISS
HSDLLALHQALELEYL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5720 Download

>pFN21AE5720  6553 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAAGACTCCCAGGATTTAAATGAACAATCAGT
AAAGAAAACGTGCACAGAATCAGATGTTTCACAATCTCAGAATTCCAGGTCTATGGAAAT
GCAGGACCTAGCAAGTCCTCATACTCTTGTTGGAGGTGGTGATACTCCAGGTAGCTCCAA
ACTGGAAAAATCTAATCTCAGCAGCACATCAGTTACTACAAATGGGACAGGAGTGTCTCT
TCTTGCAGTCAAAACAGAGCCCTTGAACAGCAGTGAAACCACAGCCACGACTGGAGATGG
AGCGCTTGACACTTTTACTGGGTCAGTAATTACAAGTAGTGGCTACAGCCCCAGATCAGC
ACATCAGTATTCCCCACAGCTGTATCCTTCCAAGCCCTATCCACACATTCTTTCTACACC
AGCAGCTCAAACAATGTCTGCCTATGCAGGCCAGACTCAGTATTCGGGGATGCAGCAGCC
AGCCGTCTACACAGCCTACTCACAGACAGGACAGCCCTACAGCTTGCCCACTTACGATTT
GGGTGTGATGTTGCCAGCCATCAAGACAGAGAGTGGACTTTCCCAAACTCAGTCCCCATT
ACAGAGTGGCTGCCTCAGTTACAGCCCAGGGTTCTCTACCCCACAGCCAGGCCAGACACC
TTATTCTTACCAAATGCCAGGTTCTAGTTTTGCACCATCATCTACTATTTATGCAAATAA
TTCAGTTTCCAATTCAACAAATTTCAGTGGTTCACAACAGGATTATCCATCCTATACAGC
CTTTGGCCAAAACCAGTATGCACAGTATTATTCAGCATCAACGTATGGAGCGTATATGAC
ATCGAATAACACAGCCGATGGCACACCCTCTTCAACCTCTACTTATCAGTTGCAGGAATC
TCTCCCAGGACTGACTAACCAACCAGGAGAGTTCGATACCATGCAGAGTCCCTCCACACC
CATCAAAGATCTTGATGAGAGAACCTGTAGGAGTTCTGGGTCAAAGTCCAGAGGAAGAGG
CCGGAAAAATAATCCCTCCCCGCCTCCTGATAGTGACCTGGAGCGTGTGTTTGTCTGGGA
TTTGGATGAAACCATCATTGTTTTTCACTCACTGCTCACCGGGTCTTATGCACAGAAGTA
TGGCAAGGATCCCCCCATGGCTGTAACCCTTGGACTCCGCATGGAAGAAATGATTTTTAA
TCTTGCTGATACTCATTTGTTTTTTAATGATTTAGAGGAGTGTGATCAAGTTCATATAGA
TGATGTTTCCTCTGATGATAATGGGCAGGACTTAAGTACCTACAGTTTTGCAACTGATGG
CTTCCATGCAGCTGCAAGTAGTGCAAACCTTTGTTTGCCAACAGGTGTAAGAGGAGGGGT
TGACTGGATGAGGAAGTTGGCTTTTCGTTACAGAAGAGTAAAAGAATTATATAACACCTA
CAAGAACAACGTTGGAGGACTCCTTGGCCCTGCCAAGAGGGATGCCTGGCTACAGTTAAG
GGCAGAGATTGAAGGTCTGACAGATTCCTGGCTAACAAATGCACTTAAGTCTTTATCAAT
TATTAGCACTAGGAGTAACTGCATAAATGTCTTGGTAACGACAACTCAACTGATCCCAGC
ACTTGCGAAGGTTCTACTCTATAGTTTAGGAGGTGCTTTCCCCATTGAGAATATTTACAG
TGCAACTAAAATAGGAAAAGAAAGTTGCTTTGAACGAATAATGCAAAGGTTTGGCAGAAA
AGTAGTGTATGTTGTAATTGGGGATGGTGTAGAAGAAGAACAGGCAGCAAAAAAGCACAA
CATGCCCTTCTGGAGGATATCCAGTCACTCAGACCTCCTGGCTCTCCACCAAGCACTGGA
ATTAGAGTATTTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT