TPSG1 ORF clone pFN21AE6356

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6356

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Clone info.

Species

human

Clone Name

pFN21AE6356 Sequence (with vector) Restriction Map

Product ID

FHC25537

ORF Sequence

DNA sequence (963 bp)
Protein sequence (321 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6356

Symbol

TPSG1

Alias : PRSS31, TMT, trpA

Description

tryptase gamma 1

Original Clone ID

oc96356

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 963 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 321 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10430.1 4.3e-134 99.7 TPSG1 CCDS10431.1 3.2e-44 46.9 TPSAB1 CCDS10481.1 9.1e-42 42.6 PRSS22 CCDS58185.1 8e-38 42.4 TMPRSS13 CCDS55788.1 4.8e-37 43.1 TMPRSS13 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001314 64 79 PR00722 Peptidase S1A IPR001314 121 135 PR00722 Peptidase S1A IPR001314 215 227 PR00722 Peptidase S1A HMMPfam IPR001254 38 265 PF00089 Peptidase S1/S6 HMMSmart IPR001254 37 265 SM00020 Peptidase S1/S6 ProfileScan IPR001254 38 270 PS50240 Peptidase S1/S6
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MALGACGLLLLLAVPGVSLRTLQ 23 PRIMARY 23 2 284 LLAGFFLPGLFLLLVSCVLLAKC 306 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_036599 321 609341 100.0 100.0 XP_011520748 346 609341 94.3 99.1 XP_011520749 346 609341 94.3 99.1 NP_077078 275 191081 47.3 81.3 NP_003285 275 191080 46.9 81.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6356 Download

>KIEE6356  963 bp
ATGGCCCTTGGGGCCTGTGGCCTCCTGCTGCTCCTGGCTGTGCCCGGTGTGTCCCTCAGG
ACTTTGCAGCCAGGGTGTGGCCGGCCGCAGGTTTCGGATGCAGGCGGCCGGATCGTGGGG
GGTCACGCTGCCCCGGCCGGCGCATGGCCATGGCAGGCCAGCCTCCGCCTGCGGAGGGTG
CACGTGTGCGGCGGGTCACTGCTCAGCCCCCAGTGGGTGCTCACAGCTGCCCACTGCTTC
TCCGGGTCCCTGAACTCATCCGACTACCAGGTGCACCTGGGGGAACTGGAGATCACTCTG
TCTCCCCACTTCTCCACCGTGAGGCAGATCATCCTGCACTCCAGCCCCTCAGGACAGCCG
GGGACCAGCGGGGACATCGCCCTGGTGGAGCTCAGTGTCCCCGTGACCCTCTCCAGCCGG
ATCCTGCCCGTCTGCCTCCCGGAGGCCTCAGATGACTTCTGCCCTGGGATCCGGTGCTGG
GTGACCGGCTGGGGCTATACGCGGGAGGGAGAGCCTCTGCCACCCCCGTACAGCCTGCGG
GAGGTGAAAGTCTCCGTGGTGGACACAGAGACCTGCCGCCGGGACTATCCCGGCCCCGGG
GGCAGCATCCTTCAGCCCGACATGCTGTGTGCCCGGGGCCCCGGGGATGCCTGCCAGGAC
GACTCCGGGGGGCCTCTGGTCTGCCAGGTGAACGGTGCCTGGGTGCAGGCTGGCATTGTG
AGCTGGGGTGAGGGCTGCGGCCGCCCCAACAGGCCGGGAGTCTACACTCGTGTCCCTGCC
TACGTGAACTGGATCCGCCGCCACATCACAGCATCAGGGGGCTCAGAGTCTGGGTACCCC
AGGCTCCCCCTCCTGGCTGGCTTCTTCCTCCCCGGCCTCTTCCTTCTGCTAGTCTCCTGT
GTCCTGCTGGCCAAGTGCCTGCTGCACCCATCTGCGGATGGTACTCCCTTCCCCGCCCCT
GAC

ORF Protein

Cloned ORF protein sequence for pFN21AE6356 Download

>KIEE6356  321 aa
MALGACGLLLLLAVPGVSLRTLQPGCGRPQVSDAGGRIVGGHAAPAGAWPWQASLRLRRV
HVCGGSLLSPQWVLTAAHCFSGSLNSSDYQVHLGELEITLSPHFSTVRQIILHSSPSGQP
GTSGDIALVELSVPVTLSSRILPVCLPEASDDFCPGIRCWVTGWGYTREGEPLPPPYSLR
EVKVSVVDTETCRRDYPGPGGSILQPDMLCARGPGDACQDDSGGPLVCQVNGAWVQAGIV
SWGEGCGRPNRPGVYTRVPAYVNWIRRHITASGGSESGYPRLPLLAGFFLPGLFLLLVSC
VLLAKCLLHPSADGTPFPAPD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6356 Download

>pFN21AE6356  5668 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCCTTGGGGCCTGTGGCCTCCTGCTGCTCCT
GGCTGTGCCCGGTGTGTCCCTCAGGACTTTGCAGCCAGGGTGTGGCCGGCCGCAGGTTTC
GGATGCAGGCGGCCGGATCGTGGGGGGTCACGCTGCCCCGGCCGGCGCATGGCCATGGCA
GGCCAGCCTCCGCCTGCGGAGGGTGCACGTGTGCGGCGGGTCACTGCTCAGCCCCCAGTG
GGTGCTCACAGCTGCCCACTGCTTCTCCGGGTCCCTGAACTCATCCGACTACCAGGTGCA
CCTGGGGGAACTGGAGATCACTCTGTCTCCCCACTTCTCCACCGTGAGGCAGATCATCCT
GCACTCCAGCCCCTCAGGACAGCCGGGGACCAGCGGGGACATCGCCCTGGTGGAGCTCAG
TGTCCCCGTGACCCTCTCCAGCCGGATCCTGCCCGTCTGCCTCCCGGAGGCCTCAGATGA
CTTCTGCCCTGGGATCCGGTGCTGGGTGACCGGCTGGGGCTATACGCGGGAGGGAGAGCC
TCTGCCACCCCCGTACAGCCTGCGGGAGGTGAAAGTCTCCGTGGTGGACACAGAGACCTG
CCGCCGGGACTATCCCGGCCCCGGGGGCAGCATCCTTCAGCCCGACATGCTGTGTGCCCG
GGGCCCCGGGGATGCCTGCCAGGACGACTCCGGGGGGCCTCTGGTCTGCCAGGTGAACGG
TGCCTGGGTGCAGGCTGGCATTGTGAGCTGGGGTGAGGGCTGCGGCCGCCCCAACAGGCC
GGGAGTCTACACTCGTGTCCCTGCCTACGTGAACTGGATCCGCCGCCACATCACAGCATC
AGGGGGCTCAGAGTCTGGGTACCCCAGGCTCCCCCTCCTGGCTGGCTTCTTCCTCCCCGG
CCTCTTCCTTCTGCTAGTCTCCTGTGTCCTGCTGGCCAAGTGCCTGCTGCACCCATCTGC
GGATGGTACTCCCTTCCCCGCCCCTGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT