SLC6A12 ORF clone pFN21AE6466

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6466

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Clone info.

Species

human

Clone Name

pFN21AE6466 Sequence (with vector) Restriction Map

Product ID

FHC24549

ORF Sequence

DNA sequence (1842 bp)
Protein sequence (614 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6466

Symbol

SLC6A12

Alias : BGT-1, BGT1, GAT2

Description

solute carrier family 6 (neurotransmitter transporter), member 12, transcript variant 1

Original Clone ID

oc96466

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1842 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 614 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8501.1 0 99.8 SLC6A12 CCDS8502.1 2.1e-192 71.5 SLC6A13 CCDS2602.1 1.5e-181 67.8 SLC6A11 CCDS33705.1 8e-164 64.0 SLC6A6 CCDS77704.1 9e-164 64.0 SLC6A6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002983 4 17 PR01198 Sodium:neurotransmitter symporter IPR000175 44 65 PR00176 Sodium:neurotransmitter symporter IPR000175 73 92 PR00176 Sodium:neurotransmitter symporter IPR000175 117 143 PR00176 Sodium:neurotransmitter symporter IPR000175 238 255 PR00176 Sodium:neurotransmitter symporter IPR000175 320 340 PR00176 Sodium:neurotransmitter symporter IPR000175 374 393 PR00176 Sodium:neurotransmitter symporter IPR000175 458 478 PR00176 Sodium:neurotransmitter symporter IPR000175 498 518 PR00176 Sodium:neurotransmitter symporter IPR002983 565 582 PR01198 Sodium:neurotransmitter symporter IPR002983 599 611 PR01198 Sodium:neurotransmitter symporter HMMPfam IPR000175 36 560 PF00209 Sodium:neurotransmitter symporter ProfileScan IPR000175 35 563 PS50267 Sodium:neurotransmitter symporter
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 74 FFIPYFIFFFVCGIPVFFLEVAL 96 PRIMARY 23 2 119 GLASVVIESYLNVYYIIILAWAL 141 PRIMARY 23 3 210 RWELALCLLLAWVICYFCIWKGV 232 PRIMARY 23 4 239 VYFTATFPYLMLVILLIRGVTLP 261 PRIMARY 23 5 287 AGTQIFFSFAICQGCLTALGSY 308 SECONDARY 22 6 320 IALCFLNSATSFVAGFVVFSILG 342 SECONDARY 23 7 387 MLIFLGLDSQFVCVECLVTASID 409 PRIMARY 23 8 424 LILTIAVMCYLIGLFLVTEGGMY 446 PRIMARY 23 9 455 ASSGICLLFLSLFEVVCISWVYG 477 PRIMARY 23 10 495 PLVKISWLFLTPGLCLATFLFSL 517 SECONDARY 23 11 538 IGWFLALSSMVCVPLFVVITLLK 560 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001116319 614 603080 99.8 100.0 NP_001193860 614 603080 99.8 100.0 XP_011519312 614 603080 99.8 100.0 NP_003035 614 603080 99.8 100.0 NP_001116320 614 603080 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6466 Download

>KIEE6466  1842 bp
ATGGACGGGAAGGTGGCAGTGCAAGAGCGTGGGCCTCCTGCGGTCTCCTGGGTCCCCGAG
GAGGGAGAGAAGTTGGACCAGGAAGACGAGGACCAGGTGAAGGATCGGGGCCAATGGACC
AACAAGATGGAGTTTGTGCTGTCAGTGGCCGGGGAGATCATTGGGCTGGGCAATGTCTGG
AGGTTTCCCTATCTCTGCTACAAAAACGGAGGTGGAGCCTTCTTCATCCCCTACTTCATC
TTCTTCTTTGTCTGCGGCATCCCGGTGTTCTTCCTGGAGGTGGCGTTGGGCCAATACACC
AGCCAAGGGAGTGTCACAGCCTGGAGGAAGATCTGCCCCCTCTTCCAGGGCATTGGTCTG
GCATCTGTGGTCATCGAGTCATATTTGAATGTCTACTACATCATCATCCTTGCCTGGGCT
CTCTTCTACCTGTTCAGCTCCTTCACTTCTGAGCTGCCCTGGACGACCTGCAACAACTTT
TGGAACACAGAGCATTGCACGGACTTTCTGAACCACTCAGGAGCCGGCACAGTGACCCCA
TTTGAGAATTTTACCTCACCTGTCATGGAATTCTGGGAGAGACGAGTTCTGGGCATCACC
TCGGGCATCCATGACCTGGGCTCCCTGCGCTGGGAGCTGGCCCTGTGCCTCCTGCTCGCC
TGGGTCATCTGCTATTTCTGCATCTGGAAGGGGGTCAAGTCCACAGGCAAGGTGGTTTAT
TTCACAGCCACGTTTCCGTACCTGATGCTTGTCATTTTGCTGATCAGAGGTGTCACCCTT
CCCGGAGCCTACCAGGGCATCATCTACTACTTGAAGCCAGATTTGTTCCGCCTCAAGGAC
CCTCAGGTGTGGATGGATGCGGGCACCCAGATCTTCTTCTCCTTTGCCATCTGCCAGGGG
TGCCTGACAGCCCTGGGCAGCTACAACAAGTATCACAACAACTGCTACAAGGACTGCATC
GCCCTCTGCTTCCTGAACAGTGCCACCAGCTTTGTGGCTGGGTTTGTTGTCTTCTCCATC
CTGGGCTTCATGTCCCAAGAGCAAGGGGTGCCCATTTCTGAAGTGGCCGAGTCAGGTCCT
GGGCTGGCCTTCATCGCCTTCCCCAAGGCTGTGACTATGATGCCCTTATCCCAGCTGTGG
TCCTGCCTGTTCTTTATCATGCTCATATTCCTAGGGCTGGACAGCCAGTTTGTCTGTGTG
GAGTGCCTGGTGACAGCCTCCATAGACATGTTCCCCAGGCAGCTCCGGAAGAGCGGGCGG
CGCGAGCTCCTCATCCTCACCATCGCCGTCATGTGCTACCTGATAGGGCTTTTCCTGGTC
ACCGAGGGCGGGATGTACATCTTCCAGCTGTTTGACTACTATGCTTCCAGTGGCATATGC
CTGCTGTTCCTGTCATTGTTTGAAGTGGTCTGCATAAGCTGGGTGTATGGGGCGGACCGT
TTCTATGACAACATTGAGGACATGATTGGCTACCGGCCATGGCCCCTGGTGAAGATCTCC
TGGCTCTTCCTGACCCCTGGACTTTGCCTGGCCACTTTCCTCTTCTCCTTGAGCAAGTAC
ACCCCCCTCAAGTACAACAACGTCTATGTGTACCCGCCCTGGGGATACTCCATTGGCTGG
TTCCTGGCTCTGTCCTCCATGGTCTGTGTCCCACTCTTCGTCGTCATCACCCTCCTGAAG
ACTCGGGGTCCTTTCAGGAAGCGTCTGCGTCAGCTCATCACCCCTGACTCCAGTCTGCCA
CAGCCCAAGCAACATCCCTGCTTGGATGGCAGTGCTGGCCGGAACTTTGGGCCCTCCCCA
ACAAGGGAAGGACTGATAGCCGGGGAGAAGGAGACCCATTTG

ORF Protein

Cloned ORF protein sequence for pFN21AE6466 Download

>KIEE6466  614 aa
MDGKVAVQERGPPAVSWVPEEGEKLDQEDEDQVKDRGQWTNKMEFVLSVAGEIIGLGNVW
RFPYLCYKNGGGAFFIPYFIFFFVCGIPVFFLEVALGQYTSQGSVTAWRKICPLFQGIGL
ASVVIESYLNVYYIIILAWALFYLFSSFTSELPWTTCNNFWNTEHCTDFLNHSGAGTVTP
FENFTSPVMEFWERRVLGITSGIHDLGSLRWELALCLLLAWVICYFCIWKGVKSTGKVVY
FTATFPYLMLVILLIRGVTLPGAYQGIIYYLKPDLFRLKDPQVWMDAGTQIFFSFAICQG
CLTALGSYNKYHNNCYKDCIALCFLNSATSFVAGFVVFSILGFMSQEQGVPISEVAESGP
GLAFIAFPKAVTMMPLSQLWSCLFFIMLIFLGLDSQFVCVECLVTASIDMFPRQLRKSGR
RELLILTIAVMCYLIGLFLVTEGGMYIFQLFDYYASSGICLLFLSLFEVVCISWVYGADR
FYDNIEDMIGYRPWPLVKISWLFLTPGLCLATFLFSLSKYTPLKYNNVYVYPPWGYSIGW
FLALSSMVCVPLFVVITLLKTRGPFRKRLRQLITPDSSLPQPKQHPCLDGSAGRNFGPSP
TREGLIAGEKETHL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6466 Download

>pFN21AE6466  6547 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACGGGAAGGTGGCAGTGCAAGAGCGTGGGCC
TCCTGCGGTCTCCTGGGTCCCCGAGGAGGGAGAGAAGTTGGACCAGGAAGACGAGGACCA
GGTGAAGGATCGGGGCCAATGGACCAACAAGATGGAGTTTGTGCTGTCAGTGGCCGGGGA
GATCATTGGGCTGGGCAATGTCTGGAGGTTTCCCTATCTCTGCTACAAAAACGGAGGTGG
AGCCTTCTTCATCCCCTACTTCATCTTCTTCTTTGTCTGCGGCATCCCGGTGTTCTTCCT
GGAGGTGGCGTTGGGCCAATACACCAGCCAAGGGAGTGTCACAGCCTGGAGGAAGATCTG
CCCCCTCTTCCAGGGCATTGGTCTGGCATCTGTGGTCATCGAGTCATATTTGAATGTCTA
CTACATCATCATCCTTGCCTGGGCTCTCTTCTACCTGTTCAGCTCCTTCACTTCTGAGCT
GCCCTGGACGACCTGCAACAACTTTTGGAACACAGAGCATTGCACGGACTTTCTGAACCA
CTCAGGAGCCGGCACAGTGACCCCATTTGAGAATTTTACCTCACCTGTCATGGAATTCTG
GGAGAGACGAGTTCTGGGCATCACCTCGGGCATCCATGACCTGGGCTCCCTGCGCTGGGA
GCTGGCCCTGTGCCTCCTGCTCGCCTGGGTCATCTGCTATTTCTGCATCTGGAAGGGGGT
CAAGTCCACAGGCAAGGTGGTTTATTTCACAGCCACGTTTCCGTACCTGATGCTTGTCAT
TTTGCTGATCAGAGGTGTCACCCTTCCCGGAGCCTACCAGGGCATCATCTACTACTTGAA
GCCAGATTTGTTCCGCCTCAAGGACCCTCAGGTGTGGATGGATGCGGGCACCCAGATCTT
CTTCTCCTTTGCCATCTGCCAGGGGTGCCTGACAGCCCTGGGCAGCTACAACAAGTATCA
CAACAACTGCTACAAGGACTGCATCGCCCTCTGCTTCCTGAACAGTGCCACCAGCTTTGT
GGCTGGGTTTGTTGTCTTCTCCATCCTGGGCTTCATGTCCCAAGAGCAAGGGGTGCCCAT
TTCTGAAGTGGCCGAGTCAGGTCCTGGGCTGGCCTTCATCGCCTTCCCCAAGGCTGTGAC
TATGATGCCCTTATCCCAGCTGTGGTCCTGCCTGTTCTTTATCATGCTCATATTCCTAGG
GCTGGACAGCCAGTTTGTCTGTGTGGAGTGCCTGGTGACAGCCTCCATAGACATGTTCCC
CAGGCAGCTCCGGAAGAGCGGGCGGCGCGAGCTCCTCATCCTCACCATCGCCGTCATGTG
CTACCTGATAGGGCTTTTCCTGGTCACCGAGGGCGGGATGTACATCTTCCAGCTGTTTGA
CTACTATGCTTCCAGTGGCATATGCCTGCTGTTCCTGTCATTGTTTGAAGTGGTCTGCAT
AAGCTGGGTGTATGGGGCGGACCGTTTCTATGACAACATTGAGGACATGATTGGCTACCG
GCCATGGCCCCTGGTGAAGATCTCCTGGCTCTTCCTGACCCCTGGACTTTGCCTGGCCAC
TTTCCTCTTCTCCTTGAGCAAGTACACCCCCCTCAAGTACAACAACGTCTATGTGTACCC
GCCCTGGGGATACTCCATTGGCTGGTTCCTGGCTCTGTCCTCCATGGTCTGTGTCCCACT
CTTCGTCGTCATCACCCTCCTGAAGACTCGGGGTCCTTTCAGGAAGCGTCTGCGTCAGCT
CATCACCCCTGACTCCAGTCTGCCACAGCCCAAGCAACATCCCTGCTTGGATGGCAGTGC
TGGCCGGAACTTTGGGCCCTCCCCAACAAGGGAAGGACTGATAGCCGGGGAGAAGGAGAC
CCATTTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT