GSPT2 ORF clone pFN21AE6784

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6784

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Clone info.

Species

human

Clone Name

pFN21AE6784 Sequence (with vector) Restriction Map

Product ID

FHC27388

ORF Sequence

DNA sequence (1884 bp)
Protein sequence (628 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6784

Symbol

GSPT2

Alias : ERF3B, GST2

Description

G1 to S phase transition 2

Original Clone ID

oc96784

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1884 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 628 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14336.1 1e-208 99.5 GSPT2 CCDS45412.1 6.4e-182 88.0 GSPT1 CCDS45413.1 6e-168 87.9 GSPT1 CCDS45414.1 1.6e-155 93.4 GSPT1 CCDS47479.1 1.5e-54 37.9 HBS1L The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000795 205 218 PR00315 Protein synthesis factor IPR000795 264 272 PR00315 Protein synthesis factor IPR000795 284 294 PR00315 Protein synthesis factor IPR000795 300 311 PR00315 Protein synthesis factor IPR000795 344 353 PR00315 Protein synthesis factor HMMPfam IPR009818 48 64 PF07145 Ataxin-2 IPR000795 201 400 PF00009 Protein synthesis factor IPR004161 443 510 PF03144 Translation elongation factor EFTu/EF1A IPR004160 520 623 PF03143 Translation elongation factor EFTu/EF1A
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060564 628 300418 99.5 100.0 NP_002085 637 139259 88.2 99.7 NP_001123478 636 139259 88.0 99.7 NP_001138630 642 612450 37.9 83.0 NP_006611 684 612450 37.9 83.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6784 Download

>KIEE6784  1884 bp
ATGGATTCGGGTAGCAGCAGCAGCGACTCGGCGCCCGATTGCTGGGACCAGGTGGACATG
GAATCCACGGGGTCGGCCCCGAGCGGGGATGGAGTCTCCTCTGCGGTGGCCGAGGCCCAG
CGCGAGCCCCTCAGCTCGGCTTTCAGCCGTAAGCTCAACGTCAACGCCAAGCCCTTCGTG
CCTAACGTACACGCCGCGGAGTTCGTGCCGTCCTTCCTGCGGGGCCCGACTCAGCCGCCC
ACCCTCCCGGCCGGCTCCGGCAGCAACGATGAAACCTGCACCGGCGCGGGATACCCTCAA
GGTAAAAGGATGGGACGGGGGGCACCTGTGGAACCTTCCCGAGAGGAACCGTTAGTGTCG
CTTGAAGGTTCCAATTCAGCCGTTACCATGGAACTTTCAGAACCTGTTGTAGAAAATGGA
GAGGTGGAAATGGCCCTAGAAGAATCATGGGAGCACAGTAAAGAAGTAAGTGAAGCCGAG
CCTGGGGGTGGTTCCTCGGGAGATTCAGGGCCCCCAGAAGAAAGTGGCCAGGAAATGATG
GAGGAAAAAGAGGAAATAAGAAAATCCAAATCTGTGATCGTACCCTCAGGTGCACCTAAG
AAAGAACACGTAAATGTAGTATTCATTGGCCATGTAGACGCTGGCAAGTCAACCATCGGA
GGACAGATAATGTTTTTGACTGGAATGGTTGACAAAAGAACACTGGAGAAATATGAAAGA
GAAGCTAAGGAAAAAAACAGAGAAACCTGGTATTTGTCCTGGGCCTTAGATACAAATCAG
GAGGAACGAGACAAGGGTAAAACAGTCGAAGTGGGTCGTGCCTATTTTGAAACAGAAAGG
AAACATTTCACAATTTTAGATGCCCCTGGCCACAAGAGTTTTGTCCCAAATATGATTGGT
GGTGCTTCTCAAGCTGATTTGGCTGTGCTGGTCATCTCTGCCAGGAAAGGAGAGTTTGAA
ACTGGATTTGAAAAAGGTGGACAGACAAGAGAACATGCGATGTTGGCAAAAACGGCAGGG
GTAAAACATTTAATAGTGCTTATTAATAAGATGGATGATCACACAGTAAATTGGAGCATC
GAGAGATATGAAGAATGTAAAGAAAAACTGGTGCCCTTTTTGAAAAAAGTAGGCTTCAGT
CCAAAAAAGGACATTCACTTTATGCCCTGCTCAGGACTGACCGGAGCAAATATTAAAGAG
CAGTCAGATTTCTGCCCTTGGTACACTGGATTACCATTTATTCCGTATTTGGATAACTTG
CCAAACTTCAACAGATCAATTGATGGACCAATAAGACTGCCAATTGTGGATAAGTACAAA
GATATGGGCACTGTGGTCCTGGGAAAGCTGGAATCCGGGTCCATTTTTAAAGGCCAGCAG
CTCGTGATGATGCCAAACAAGCACAATGTAGAAGTTCTTGGAATACTTTCTGATGATACT
GAAACTGATTTTGTAGCCCCAGGTGAAAACCTCAAAATCAGACTGAAGGGAATTGAAGAA
GAAGAGATTCTTCCAGGATTCATACTTTGTGATCCTAGTAACCTCTGCCATTCTGGACGC
ACGTTTGATGTTCAGATAGTGATTATTGAGCACAAATCCATCATCTGCCCAGGTTATAAT
GCGGTGCTGCACATTCATACTTGTATTGAGGAAGTTGAGATAACAGCGTTAATCTCCTTG
GTAGACAAAAAATCAGGAGAAAAAAGTAAGACACGACCCCGCTTCGTGAAACAAGATCAA
GTATGCATTGCTCGTTTAAGGACAGCAGGAACCATCTGCCTCGAGACGTTCAAAGATTTT
CCTCAGATGGGTCGTTTTACTTTAAGAGATGAGGGTAAGACCATTGCAATTGGAAAAGTT
CTGAAATTGGTCCAAGAGAAGGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE6784 Download

>KIEE6784  628 aa
MDSGSSSSDSAPDCWDQVDMESTGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFV
PNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVS
LEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMM
EEKEEIRKSKSVIVPSGAPKKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYER
EAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIG
GASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDHTVNWSI
ERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDNL
PNFNRSIDGPIRLPIVDKYKDMGTVVLGKLESGSIFKGQQLVMMPNKHNVEVLGILSDDT
ETDFVAPGENLKIRLKGIEEEEILPGFILCDPSNLCHSGRTFDVQIVIIEHKSIICPGYN
AVLHIHTCIEEVEITALISLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDF
PQMGRFTLRDEGKTIAIGKVLKLVQEKD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6784 Download

>pFN21AE6784  6589 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATTCGGGTAGCAGCAGCAGCGACTCGGCGCC
CGATTGCTGGGACCAGGTGGACATGGAATCCACGGGGTCGGCCCCGAGCGGGGATGGAGT
CTCCTCTGCGGTGGCCGAGGCCCAGCGCGAGCCCCTCAGCTCGGCTTTCAGCCGTAAGCT
CAACGTCAACGCCAAGCCCTTCGTGCCTAACGTACACGCCGCGGAGTTCGTGCCGTCCTT
CCTGCGGGGCCCGACTCAGCCGCCCACCCTCCCGGCCGGCTCCGGCAGCAACGATGAAAC
CTGCACCGGCGCGGGATACCCTCAAGGTAAAAGGATGGGACGGGGGGCACCTGTGGAACC
TTCCCGAGAGGAACCGTTAGTGTCGCTTGAAGGTTCCAATTCAGCCGTTACCATGGAACT
TTCAGAACCTGTTGTAGAAAATGGAGAGGTGGAAATGGCCCTAGAAGAATCATGGGAGCA
CAGTAAAGAAGTAAGTGAAGCCGAGCCTGGGGGTGGTTCCTCGGGAGATTCAGGGCCCCC
AGAAGAAAGTGGCCAGGAAATGATGGAGGAAAAAGAGGAAATAAGAAAATCCAAATCTGT
GATCGTACCCTCAGGTGCACCTAAGAAAGAACACGTAAATGTAGTATTCATTGGCCATGT
AGACGCTGGCAAGTCAACCATCGGAGGACAGATAATGTTTTTGACTGGAATGGTTGACAA
AAGAACACTGGAGAAATATGAAAGAGAAGCTAAGGAAAAAAACAGAGAAACCTGGTATTT
GTCCTGGGCCTTAGATACAAATCAGGAGGAACGAGACAAGGGTAAAACAGTCGAAGTGGG
TCGTGCCTATTTTGAAACAGAAAGGAAACATTTCACAATTTTAGATGCCCCTGGCCACAA
GAGTTTTGTCCCAAATATGATTGGTGGTGCTTCTCAAGCTGATTTGGCTGTGCTGGTCAT
CTCTGCCAGGAAAGGAGAGTTTGAAACTGGATTTGAAAAAGGTGGACAGACAAGAGAACA
TGCGATGTTGGCAAAAACGGCAGGGGTAAAACATTTAATAGTGCTTATTAATAAGATGGA
TGATCACACAGTAAATTGGAGCATCGAGAGATATGAAGAATGTAAAGAAAAACTGGTGCC
CTTTTTGAAAAAAGTAGGCTTCAGTCCAAAAAAGGACATTCACTTTATGCCCTGCTCAGG
ACTGACCGGAGCAAATATTAAAGAGCAGTCAGATTTCTGCCCTTGGTACACTGGATTACC
ATTTATTCCGTATTTGGATAACTTGCCAAACTTCAACAGATCAATTGATGGACCAATAAG
ACTGCCAATTGTGGATAAGTACAAAGATATGGGCACTGTGGTCCTGGGAAAGCTGGAATC
CGGGTCCATTTTTAAAGGCCAGCAGCTCGTGATGATGCCAAACAAGCACAATGTAGAAGT
TCTTGGAATACTTTCTGATGATACTGAAACTGATTTTGTAGCCCCAGGTGAAAACCTCAA
AATCAGACTGAAGGGAATTGAAGAAGAAGAGATTCTTCCAGGATTCATACTTTGTGATCC
TAGTAACCTCTGCCATTCTGGACGCACGTTTGATGTTCAGATAGTGATTATTGAGCACAA
ATCCATCATCTGCCCAGGTTATAATGCGGTGCTGCACATTCATACTTGTATTGAGGAAGT
TGAGATAACAGCGTTAATCTCCTTGGTAGACAAAAAATCAGGAGAAAAAAGTAAGACACG
ACCCCGCTTCGTGAAACAAGATCAAGTATGCATTGCTCGTTTAAGGACAGCAGGAACCAT
CTGCCTCGAGACGTTCAAAGATTTTCCTCAGATGGGTCGTTTTACTTTAAGAGATGAGGG
TAAGACCATTGCAATTGGAAAAGTTCTGAAATTGGTCCAAGAGAAGGACGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT