MAS1L ORF clone pFN21AE9418

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9418

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Clone info.

Species

human

Clone Name

pFN21AE9418 Sequence (with vector) Restriction Map

Product ID

FHC22531

ORF Sequence

DNA sequence (1134 bp)
Protein sequence (378 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9418

Symbol

MAS1L

Alias : MAS-L, MRG, dJ994E9.2

Description

MAS1 proto-oncogene like, G protein-coupled receptor

Original Clone ID

cp99418

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1134 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 378 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4661.1 1.8e-149 100.0 MAS1L CCDS5272.1 2.3e-28 32.5 MAS1 CCDS41603.2 6.6e-23 31.7 MRGPRE CCDS8188.1 9.4e-23 30.4 MRGPRF CCDS7846.1 2.1e-22 39.2 MRGPRX1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 77 101 PR00237 GPCR IPR000276 108 129 PR00237 GPCR IPR000276 153 175 PR00237 GPCR IPR000276 296 322 PR00237 GPCR HMMPfam IPR000276 93 314 PF00001 GPCR ProfileScan IPR017452 82 314 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MVWGKICWFSQRAGWTVFAESQI 23 SECONDARY 23 2 77 IIAPKAVLVSLCGVLLNGTVFWL 99 PRIMARY 23 3 108 YMVYILHLVAADVIYLCCSAVGF 130 PRIMARY 23 4 135 LLTYHGVVFFIPDFLAILSPFSF 157 PRIMARY 23 5 161 LCLLVAISTERCVCVLFPIWYRC 183 PRIMARY 23 6 191 NVVCTLIWGLPFCINIVKSLFLT 213 PRIMARY 23 7 225 LKLSGLFHAILSLVMCVSSLTLL 247 PRIMARY 23 8 260 TRVYAVVQISAPMFLLWALPLSV 282 PRIMARY 23 9 287 TDFKMFVTTSYLISLFLIINSSA 309 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_443199 378 607235 100.0 100.0 NP_002368 325 165180 32.5 80.2 NP_659452 343 607233 30.4 87.0 XP_016872659 343 607233 30.4 87.0 NP_001091985 343 607233 30.4 87.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9418 Download

>KIEE9418  1134 bp
ATGGTCTGGGGGAAAATTTGCTGGTTCAGCCAGAGGGCTGGATGGACAGTGTTTGCTGAG
TCACAGATATCTCTCTCATGTAGCCTTTGTCTCCACAGTGGTGACCAGGAGGCACAGAAC
CCAAACCTGGTATCTCAGCTCTGTGGCGTCTTTCTTCAAAATGAGACGAATGAAACCATA
CATATGCAGATGAGCATGGCAGTGGGACAGCAGGCCCTGCCCTTGAATATCATTGCCCCC
AAGGCTGTGCTGGTCTCCCTCTGTGGGGTCTTATTGAATGGCACTGTCTTCTGGCTGCTT
TGCTGTGGGGCCACGAATCCCTACATGGTATACATCCTCCACCTGGTCGCTGCTGACGTG
ATCTATCTTTGCTGCTCGGCAGTGGGGTTCTTACAGGTGACTCTGCTAACTTATCATGGA
GTCGTGTTTTTTATCCCTGATTTCCTGGCCATATTGTCTCCCTTCTCCTTTGAGGTGTGT
CTCTGTCTCCTGGTGGCCATCAGCACAGAGCGGTGTGTGTGTGTCCTCTTCCCCATCTGG
TACAGATGCCACCGCCCAAAATACACATCTAATGTTGTCTGCACCCTCATCTGGGGCCTG
CCTTTTTGCATCAACATAGTAAAATCACTTTTCCTAACTTACTGGAAACATGTAAAGGCA
TGTGTCATATTTCTAAAGCTTTCTGGGCTCTTCCATGCTATCCTTTCACTTGTGATGTGT
GTGTCGAGTCTGACTCTACTCATTAGATTCCTGTGCTGCTCCCAGCAGCAAAAGGCCACC
AGGGTCTATGCGGTGGTGCAGATCTCGGCCCCCATGTTCCTACTCTGGGCCCTACCCCTG
AGCGTGGCACCCCTCATAACAGATTTCAAAATGTTTGTCACCACCTCCTATTTAATTTCC
TTGTTCCTCATTATAAACAGCAGCGCCAACCCTATCATTTATTTCTTTGTGGGGAGCCTC
AGAAAGAAAAGGCTGAAGGAATCTCTCAGAGTGATTCTCCAACGGGCGTTAGCAGATAAG
CCAGAGGTGGGGAGGAACAAAAAGGCAGCTGGCATCGACCCAATGGAGCAACCACACTCT
ACTCAGCATGTGGAGAACCTTCTTCCCAGGGAGCACAGGGTCGATGTGGAAACA

ORF Protein

Cloned ORF protein sequence for pFN21AE9418 Download

>KIEE9418  378 aa
MVWGKICWFSQRAGWTVFAESQISLSCSLCLHSGDQEAQNPNLVSQLCGVFLQNETNETI
HMQMSMAVGQQALPLNIIAPKAVLVSLCGVLLNGTVFWLLCCGATNPYMVYILHLVAADV
IYLCCSAVGFLQVTLLTYHGVVFFIPDFLAILSPFSFEVCLCLLVAISTERCVCVLFPIW
YRCHRPKYTSNVVCTLIWGLPFCINIVKSLFLTYWKHVKACVIFLKLSGLFHAILSLVMC
VSSLTLLIRFLCCSQQQKATRVYAVVQISAPMFLLWALPLSVAPLITDFKMFVTTSYLIS
LFLIINSSANPIIYFFVGSLRKKRLKESLRVILQRALADKPEVGRNKKAAGIDPMEQPHS
TQHVENLLPREHRVDVET

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9418 Download

>pFN21AE9418  5839 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTCTGGGGGAAAATTTGCTGGTTCAGCCAGAG
GGCTGGATGGACAGTGTTTGCTGAGTCACAGATATCTCTCTCATGTAGCCTTTGTCTCCA
CAGTGGTGACCAGGAGGCACAGAACCCAAACCTGGTATCTCAGCTCTGTGGCGTCTTTCT
TCAAAATGAGACGAATGAAACCATACATATGCAGATGAGCATGGCAGTGGGACAGCAGGC
CCTGCCCTTGAATATCATTGCCCCCAAGGCTGTGCTGGTCTCCCTCTGTGGGGTCTTATT
GAATGGCACTGTCTTCTGGCTGCTTTGCTGTGGGGCCACGAATCCCTACATGGTATACAT
CCTCCACCTGGTCGCTGCTGACGTGATCTATCTTTGCTGCTCGGCAGTGGGGTTCTTACA
GGTGACTCTGCTAACTTATCATGGAGTCGTGTTTTTTATCCCTGATTTCCTGGCCATATT
GTCTCCCTTCTCCTTTGAGGTGTGTCTCTGTCTCCTGGTGGCCATCAGCACAGAGCGGTG
TGTGTGTGTCCTCTTCCCCATCTGGTACAGATGCCACCGCCCAAAATACACATCTAATGT
TGTCTGCACCCTCATCTGGGGCCTGCCTTTTTGCATCAACATAGTAAAATCACTTTTCCT
AACTTACTGGAAACATGTAAAGGCATGTGTCATATTTCTAAAGCTTTCTGGGCTCTTCCA
TGCTATCCTTTCACTTGTGATGTGTGTGTCGAGTCTGACTCTACTCATTAGATTCCTGTG
CTGCTCCCAGCAGCAAAAGGCCACCAGGGTCTATGCGGTGGTGCAGATCTCGGCCCCCAT
GTTCCTACTCTGGGCCCTACCCCTGAGCGTGGCACCCCTCATAACAGATTTCAAAATGTT
TGTCACCACCTCCTATTTAATTTCCTTGTTCCTCATTATAAACAGCAGCGCCAACCCTAT
CATTTATTTCTTTGTGGGGAGCCTCAGAAAGAAAAGGCTGAAGGAATCTCTCAGAGTGAT
TCTCCAACGGGCGTTAGCAGATAAGCCAGAGGTGGGGAGGAACAAAAAGGCAGCTGGCAT
CGACCCAATGGAGCAACCACACTCTACTCAGCATGTGGAGAACCTTCTTCCCAGGGAGCA
CAGGGTCGATGTGGAAACAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT