GPR149 ORF clone pFN21AE9458

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9458

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Clone info.

Species

human

Clone Name

pFN21AE9458 Sequence (with vector) Restriction Map

Product ID

FHC30093

ORF Sequence

DNA sequence (2193 bp)
Protein sequence (731 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9458

Symbol

GPR149

Alias : IEDA, PGR10, R35

Description

G protein-coupled receptor 149

Original Clone ID

cp99458

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2193 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 731 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS43162.1 0 100.0 GPR149 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000276 59 351 PF00001 GPCR ProfileScan IPR017452 50 362 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 35 YLFCLTCLMTFAALVGSIYSLIS 57 PRIMARY 23 2 68 SMLVASWSVDDLMSVLSVTIFMF 90 SECONDARY 23 3 150 VLGVVLTVWAASLLLSALPLCGW 172 PRIMARY 23 4 188 SSYVLFLSIVYALAFGLLVGLSV 210 PRIMARY 23 5 314 ILALTKVVLWLPMMMHMVVQNVV 336 PRIMARY 23 6 351 TLLATTVTPVFVLSKRWTHLPCG 373 SECONDARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9458 Download

>KIEE9458  2193 bp
ATGTCTTTATTTCTCAGTAACTTATCAACAAATGACTCTAGCCTGTGGAAAGAGAATCAT
AATTCTACGGACCTTTTAAATCCGCCAGGAACCCTGAATATCTATCTTTTTTGCTTGACA
TGTCTCATGACTTTTGCAGCCTTGGTGGGCAGCATTTATTCACTAATTTCCCTGCTGAAA
ATGCAGAACAGAACTGTTGTGTCCATGCTTGTGGCTTCCTGGTCTGTGGATGATCTCATG
AGCGTCCTGTCGGTGACCATCTTCATGTTTTTGCAGTGGCCAAACGAGGTCCCCGGTTAC
TTCCAATTTCTGTGCACCACCTCTGCCTTAATGTATTTATGCCAGGGCCTCTCTAGCAAC
TTGAAGGCGACTCTCCTAGTCTCTTACAACTTTTATACGATGCACAGAGGTGTGGGGAGC
CAGACAGCCTCCAGAAGATCGGGCCAGGTGCTCGGCGTGGTGCTGACCGTGTGGGCAGCC
AGTCTGCTGCTCTCGGCGCTCCCGCTGTGCGGCTGGGGCGCCTTCGTGCGCACGCCCTGG
GGCTGCCTGGTGGACTGCTCCAGCTCCTACGTACTATTCCTCTCTATCGTGTACGCTTTG
GCCTTCGGACTCCTCGTGGGCCTCTCAGTCCCACTCACTCACCGATTGCTGTGTTCGGAG
GAGCCGCCGAGACTCCACTCCAACTACCAGGAAATTTCCCGTGGAGCTTCAATTCCTGGG
ACCCCTCCTACTGCGGGGAGAGTGGTTTCCCTGTCCCCAGAGGATGCTCCAGGCCCGAGT
CTGCGGCGCTCTGGGGGATGCTCTCCGAGCTCCGACACCGTGTTCGGACCGGGTGCGCCC
GCTGCCGCTGGGGCTGAAGCCTGCAGGCGTGAGAACCGGGGGACTCTCTATGGCACCAGG
AGCTTCACCGTGAGCGTAGCGCAGAAGCGCTTCGCTTTGATCCTAGCGCTTACAAAAGTC
GTCCTTTGGCTGCCCATGATGATGCACATGGTGGTCCAGAACGTCGTGGGGTTTCAGAGC
CTTCCCTTGGAGACATTCAGCTTTCTACTTACCCTGCTGGCCACCACTGTAACCCCAGTG
TTTGTCTTGTCCAAACGCTGGACCCACTTGCCCTGTGGCTGCATCATCAACTGCAGGCAG
AACGCATATGCAGTGGCGTCCGATGGGAAAAAAATCAAGAGAAAAGGCTTTGAATTCAAT
CTATCATTCCAAAAAAGTTATGGGATTTATAAAATAGCACATGAAGATTACTATGATGAT
GATGAAAATTCCATATTCTATCACAACCTGATGAACTCTGAGTGTGAAACTACAAAAGAC
CCTCAGAGAGACAACCGTAACATCTTCAATGCTATAAAAGTAGAAATCAGCACCACGCCC
TCTCTGGACAGCTCCACACAAAGAGGCATCAACAAATGCACAAATACTGATATTACAGAA
GCTAAACAGGATTCCAACAACAAAAAGGATGCGTTTTCTGACAAAACAGGAGGTGATATT
AACTATGAAGAAACTACCTTTTCTGAAGGGCCAGAAAGAAGACTGTCTCATGAAGAGAGT
CAGAAACCAGATCTTTCAGACTGGGAGTGGTGTAGGAGTAAATCAGAAAGAACCCCTCGT
CAGCGTTCCGGTTATGCCCTTGCCATTCCCTTGTGTGCATTCCAGGGGACTGTGTCTCTC
CATGCACCTACAGGGAAAACCCTATCTCTTTCTACCTATGAGGTAAGCGCAGAAGGGCAA
AAAATAACTCCAGCCTCTAAGAAAATAGAAGTCTATCGATCCAAAAGTGTTGGCCATGAA
CCAAACTCAGAAGATTCTTCATCCACGTTTGTGGACACCAGTGTGAAAATACACTTGGAG
GTTCTTGAAATTTGTGATAATGAAGAGGCCTTGGACACTGTGTCAATCATTAGTAACATC
AGTCAGTCCTCCACACAAGTCAGATCTCCATCCCTACGTTACTCCAGGAAAGAAAACAGA
TTTGTTTCATGTGACCTAGGGGAAACAGCCTCATACTCCCTCTTTTTGCCCACCAGTAAT
CCTGATGGTGATATTAATATCTCCATTCCAGACACAGTAGAAGCACACAGGCAGAACAGT
AAAAGGCAGCATCAAGAGAGGGATGGCTACCAGGAGGAAATCCAGTTGTTAAATAAAGCT
TACAGAAAAAGAGAGGAAGAAAGCAAGGGTAGT

ORF Protein

Cloned ORF protein sequence for pFN21AE9458 Download

>KIEE9458  731 aa
MSLFLSNLSTNDSSLWKENHNSTDLLNPPGTLNIYLFCLTCLMTFAALVGSIYSLISLLK
MQNRTVVSMLVASWSVDDLMSVLSVTIFMFLQWPNEVPGYFQFLCTTSALMYLCQGLSSN
LKATLLVSYNFYTMHRGVGSQTASRRSGQVLGVVLTVWAASLLLSALPLCGWGAFVRTPW
GCLVDCSSSYVLFLSIVYALAFGLLVGLSVPLTHRLLCSEEPPRLHSNYQEISRGASIPG
TPPTAGRVVSLSPEDAPGPSLRRSGGCSPSSDTVFGPGAPAAAGAEACRRENRGTLYGTR
SFTVSVAQKRFALILALTKVVLWLPMMMHMVVQNVVGFQSLPLETFSFLLTLLATTVTPV
FVLSKRWTHLPCGCIINCRQNAYAVASDGKKIKRKGFEFNLSFQKSYGIYKIAHEDYYDD
DENSIFYHNLMNSECETTKDPQRDNRNIFNAIKVEISTTPSLDSSTQRGINKCTNTDITE
AKQDSNNKKDAFSDKTGGDINYEETTFSEGPERRLSHEESQKPDLSDWEWCRSKSERTPR
QRSGYALAIPLCAFQGTVSLHAPTGKTLSLSTYEVSAEGQKITPASKKIEVYRSKSVGHE
PNSEDSSSTFVDTSVKIHLEVLEICDNEEALDTVSIISNISQSSTQVRSPSLRYSRKENR
FVSCDLGETASYSLFLPTSNPDGDINISIPDTVEAHRQNSKRQHQERDGYQEEIQLLNKA
YRKREEESKGS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9458 Download

>pFN21AE9458  6898 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCTTTATTTCTCAGTAACTTATCAACAAATGA
CTCTAGCCTGTGGAAAGAGAATCATAATTCTACGGACCTTTTAAATCCGCCAGGAACCCT
GAATATCTATCTTTTTTGCTTGACATGTCTCATGACTTTTGCAGCCTTGGTGGGCAGCAT
TTATTCACTAATTTCCCTGCTGAAAATGCAGAACAGAACTGTTGTGTCCATGCTTGTGGC
TTCCTGGTCTGTGGATGATCTCATGAGCGTCCTGTCGGTGACCATCTTCATGTTTTTGCA
GTGGCCAAACGAGGTCCCCGGTTACTTCCAATTTCTGTGCACCACCTCTGCCTTAATGTA
TTTATGCCAGGGCCTCTCTAGCAACTTGAAGGCGACTCTCCTAGTCTCTTACAACTTTTA
TACGATGCACAGAGGTGTGGGGAGCCAGACAGCCTCCAGAAGATCGGGCCAGGTGCTCGG
CGTGGTGCTGACCGTGTGGGCAGCCAGTCTGCTGCTCTCGGCGCTCCCGCTGTGCGGCTG
GGGCGCCTTCGTGCGCACGCCCTGGGGCTGCCTGGTGGACTGCTCCAGCTCCTACGTACT
ATTCCTCTCTATCGTGTACGCTTTGGCCTTCGGACTCCTCGTGGGCCTCTCAGTCCCACT
CACTCACCGATTGCTGTGTTCGGAGGAGCCGCCGAGACTCCACTCCAACTACCAGGAAAT
TTCCCGTGGAGCTTCAATTCCTGGGACCCCTCCTACTGCGGGGAGAGTGGTTTCCCTGTC
CCCAGAGGATGCTCCAGGCCCGAGTCTGCGGCGCTCTGGGGGATGCTCTCCGAGCTCCGA
CACCGTGTTCGGACCGGGTGCGCCCGCTGCCGCTGGGGCTGAAGCCTGCAGGCGTGAGAA
CCGGGGGACTCTCTATGGCACCAGGAGCTTCACCGTGAGCGTAGCGCAGAAGCGCTTCGC
TTTGATCCTAGCGCTTACAAAAGTCGTCCTTTGGCTGCCCATGATGATGCACATGGTGGT
CCAGAACGTCGTGGGGTTTCAGAGCCTTCCCTTGGAGACATTCAGCTTTCTACTTACCCT
GCTGGCCACCACTGTAACCCCAGTGTTTGTCTTGTCCAAACGCTGGACCCACTTGCCCTG
TGGCTGCATCATCAACTGCAGGCAGAACGCATATGCAGTGGCGTCCGATGGGAAAAAAAT
CAAGAGAAAAGGCTTTGAATTCAATCTATCATTCCAAAAAAGTTATGGGATTTATAAAAT
AGCACATGAAGATTACTATGATGATGATGAAAATTCCATATTCTATCACAACCTGATGAA
CTCTGAGTGTGAAACTACAAAAGACCCTCAGAGAGACAACCGTAACATCTTCAATGCTAT
AAAAGTAGAAATCAGCACCACGCCCTCTCTGGACAGCTCCACACAAAGAGGCATCAACAA
ATGCACAAATACTGATATTACAGAAGCTAAACAGGATTCCAACAACAAAAAGGATGCGTT
TTCTGACAAAACAGGAGGTGATATTAACTATGAAGAAACTACCTTTTCTGAAGGGCCAGA
AAGAAGACTGTCTCATGAAGAGAGTCAGAAACCAGATCTTTCAGACTGGGAGTGGTGTAG
GAGTAAATCAGAAAGAACCCCTCGTCAGCGTTCCGGTTATGCCCTTGCCATTCCCTTGTG
TGCATTCCAGGGGACTGTGTCTCTCCATGCACCTACAGGGAAAACCCTATCTCTTTCTAC
CTATGAGGTAAGCGCAGAAGGGCAAAAAATAACTCCAGCCTCTAAGAAAATAGAAGTCTA
TCGATCCAAAAGTGTTGGCCATGAACCAAACTCAGAAGATTCTTCATCCACGTTTGTGGA
CACCAGTGTGAAAATACACTTGGAGGTTCTTGAAATTTGTGATAATGAAGAGGCCTTGGA
CACTGTGTCAATCATTAGTAACATCAGTCAGTCCTCCACACAAGTCAGATCTCCATCCCT
ACGTTACTCCAGGAAAGAAAACAGATTTGTTTCATGTGACCTAGGGGAAACAGCCTCATA
CTCCCTCTTTTTGCCCACCAGTAATCCTGATGGTGATATTAATATCTCCATTCCAGACAC
AGTAGAAGCACACAGGCAGAACAGTAAAAGGCAGCATCAAGAGAGGGATGGCTACCAGGA
GGAAATCCAGTTGTTAAATAAAGCTTACAGAAAAAGAGAGGAAGAAAGCAAGGGTAGTGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT