TPRA1 ORF clone pFN21AE9464

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9464

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Clone info.

Species

human

Clone Name

pFN21AE9464 Sequence (with vector) Restriction Map

Product ID

FHC21679

ORF Sequence

DNA sequence (1119 bp)
Protein sequence (373 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9464

Symbol

TPRA1

Alias : GPR175, TMEM227, TPRA40

Description

transmembrane protein, adipocyte asscociated 1, transcript variant 1

Original Clone ID

cp99464

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1119 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 373 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3042.1 1.1e-165 100.0 TPRA1 CCDS46899.1 4e-86 99.5 TPRA1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018781 35 312 PF10160 Uncharacterised protein family
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 45 YWDLLLLIPNVLFLIFLLWKLPS 67 PRIMARY 23 2 79 IFITFYILVFVVALVGIARAVVS 101 PRIMARY 23 3 116 ILWEITRFFLLAIELSVIILGLA 138 PRIMARY 23 4 151 VLAITTVLSLAYSVTQGTLEILY 173 SECONDARY 23 5 192 QFWLVSSCFFFLVYSLVVILPKT 214 PRIMARY 23 6 234 ILALLNLLQGLGSVLLCFDIIEG 256 PRIMARY 23 7 266 LYFSFFAPLIYVAFLRGFFGSEP 288 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011510724 373 608336 100.0 100.0 XP_006713559 373 608336 100.0 100.0 XP_006713558 373 608336 100.0 100.0 XP_016861196 373 608336 100.0 100.0 XP_016861197 373 608336 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9464 Download

>KIEE9464  1119 bp
ATGGACACCCTGGAGGAGGTGACTTGGGCCAATGGGAGCACAGCGCTACCCCCACCCCTG
GCACCAAACATCAGTGTGCCTCATCGCTGCCTGCTGCTGCTCTACGAAGACATTGGCACC
TCCAGGGTCCGGTACTGGGACCTCTTGCTGCTCATCCCCAATGTGCTCTTCCTCATCTTC
CTGCTCTGGAAGCTTCCATCTGCTCGGGCGAAGATCCGCATCACCTCCAGCCCCATTTTT
ATCACCTTCTACATCCTGGTGTTTGTGGTGGCGCTGGTGGGCATTGCCCGGGCCGTGGTA
TCCATGACGGTGAGCACCTCGAACGCTGCAACTGTTGCTGATAAGATCCTGTGGGAGATC
ACCCGCTTCTTCCTGCTGGCCATCGAGCTGAGTGTGATCATCCTGGGCCTGGCCTTTGGC
CACCTGGAGAGTAAGTCCAGCATCAAGCGGGTGCTGGCCATCACCACAGTGCTGTCCCTG
GCCTACTCTGTCACCCAGGGGACCCTGGAGATCCTGTACCCTGATGCCCATCTCTCAGCT
GAGGACTTTAATATCTATGGCCATGGGGGCCGCCAGTTCTGGCTGGTCAGCTCCTGCTTC
TTCTTCCTGGTCTACTCTCTGGTGGTCATCCTTCCCAAGACCCCGCTGAAGGAGCGCATC
TCCCTGCCTTCTCGGAGGAGCTTCTACGTGTATGCGGGCATCCTGGCACTGCTCAACCTA
CTGCAGGGGCTGGGGAGTGTGCTGCTGTGCTTCGACATCATCGAGGGGCTCTGCTGTGTA
GATGCCACAACCTTCCTGTACTTCAGCTTCTTCGCTCCGCTCATCTACGTGGCTTTCCTC
CGGGGCTTCTTCGGCTCGGAGCCCAAGATCCTCTTCTCCTACAAATGCCAAGTGGACGAG
ACAGAGGAGCCAGATGTACACCTACCCCAGCCCTACGCTGTGGCCCGGCGGGAGGGCCTG
GAGGCTGCAGGGGCTGCTGGGGCCTCAGCTGCCAGCTACTCGAGCACGCAGTTCGACTCT
GCCGGCGGGGTGGCCTACCTGGATGACATCGCTTCCATGCCCTGCCACACTGGCAGCATC
AACAGCACAGACAGCGAGCGCTGGAAGGCCATCAATGCC

ORF Protein

Cloned ORF protein sequence for pFN21AE9464 Download

>KIEE9464  373 aa
MDTLEEVTWANGSTALPPPLAPNISVPHRCLLLLYEDIGTSRVRYWDLLLLIPNVLFLIF
LLWKLPSARAKIRITSSPIFITFYILVFVVALVGIARAVVSMTVSTSNAATVADKILWEI
TRFFLLAIELSVIILGLAFGHLESKSSIKRVLAITTVLSLAYSVTQGTLEILYPDAHLSA
EDFNIYGHGGRQFWLVSSCFFFLVYSLVVILPKTPLKERISLPSRRSFYVYAGILALLNL
LQGLGSVLLCFDIIEGLCCVDATTFLYFSFFAPLIYVAFLRGFFGSEPKILFSYKCQVDE
TEEPDVHLPQPYAVARREGLEAAGAAGASAASYSSTQFDSAGGVAYLDDIASMPCHTGSI
NSTDSERWKAINA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9464 Download

>pFN21AE9464  5824 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACACCCTGGAGGAGGTGACTTGGGCCAATGG
GAGCACAGCGCTACCCCCACCCCTGGCACCAAACATCAGTGTGCCTCATCGCTGCCTGCT
GCTGCTCTACGAAGACATTGGCACCTCCAGGGTCCGGTACTGGGACCTCTTGCTGCTCAT
CCCCAATGTGCTCTTCCTCATCTTCCTGCTCTGGAAGCTTCCATCTGCTCGGGCGAAGAT
CCGCATCACCTCCAGCCCCATTTTTATCACCTTCTACATCCTGGTGTTTGTGGTGGCGCT
GGTGGGCATTGCCCGGGCCGTGGTATCCATGACGGTGAGCACCTCGAACGCTGCAACTGT
TGCTGATAAGATCCTGTGGGAGATCACCCGCTTCTTCCTGCTGGCCATCGAGCTGAGTGT
GATCATCCTGGGCCTGGCCTTTGGCCACCTGGAGAGTAAGTCCAGCATCAAGCGGGTGCT
GGCCATCACCACAGTGCTGTCCCTGGCCTACTCTGTCACCCAGGGGACCCTGGAGATCCT
GTACCCTGATGCCCATCTCTCAGCTGAGGACTTTAATATCTATGGCCATGGGGGCCGCCA
GTTCTGGCTGGTCAGCTCCTGCTTCTTCTTCCTGGTCTACTCTCTGGTGGTCATCCTTCC
CAAGACCCCGCTGAAGGAGCGCATCTCCCTGCCTTCTCGGAGGAGCTTCTACGTGTATGC
GGGCATCCTGGCACTGCTCAACCTACTGCAGGGGCTGGGGAGTGTGCTGCTGTGCTTCGA
CATCATCGAGGGGCTCTGCTGTGTAGATGCCACAACCTTCCTGTACTTCAGCTTCTTCGC
TCCGCTCATCTACGTGGCTTTCCTCCGGGGCTTCTTCGGCTCGGAGCCCAAGATCCTCTT
CTCCTACAAATGCCAAGTGGACGAGACAGAGGAGCCAGATGTACACCTACCCCAGCCCTA
CGCTGTGGCCCGGCGGGAGGGCCTGGAGGCTGCAGGGGCTGCTGGGGCCTCAGCTGCCAG
CTACTCGAGCACGCAGTTCGACTCTGCCGGCGGGGTGGCCTACCTGGATGACATCGCTTC
CATGCCCTGCCACACTGGCAGCATCAACAGCACAGACAGCGAGCGCTGGAAGGCCATCAA
TGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT