MRGPRX4 ORF clone pFN21AE9508

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9508

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Clone info.

Species

human

Clone Name

pFN21AE9508 Sequence (with vector) Restriction Map

Product ID

FHC12617

ORF Sequence

DNA sequence (966 bp)
Protein sequence (322 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9508

Symbol

MRGPRX4

Alias : GPCR, MRGX4, SNSR6

Description

MAS-related GPR, member X4

Original Clone ID

cp99508

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 966 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 322 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7831.1 3.2e-106 99.7 MRGPRX4 CCDS7830.1 1.3e-86 82.8 MRGPRX3 CCDS7846.1 9.4e-82 79.7 MRGPRX1 CCDS7847.1 1.5e-61 62.6 MRGPRX2 CCDS31625.1 1.3e-32 42.2 MRGPRD The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 29 53 PR00237 GPCR IPR000276 61 82 PR00237 GPCR IPR000276 102 124 PR00237 GPCR IPR000276 214 238 PR00237 GPCR IPR000276 254 280 PR00237 GPCR HMMPfam IPR000276 44 272 PF00001 GPCR ProfileScan IPR017452 44 272 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 30 FTVLTCIISLVGLTGNAVVLWLL 52 PRIMARY 23 2 64 YILNLAAADFLFLSFQIIRSPLR 86 SECONDARY 23 3 97 ILVSVMTFPYFTGLSMLSAISTE 119 PRIMARY 23 4 135 PTHLSAVVCVLLWGLSLLFSMLE 157 PRIMARY 23 5 180 PVAWLIFLCVVLCVSSLVLLVRI 202 PRIMARY 23 6 216 VTILLTVLVFLLCGLPFGILGAL 238 PRIMARY 23 7 251 CHVYLVCMSLSSLNSSANPIIYF 273 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_473373 322 607230 99.7 100.0 XP_011518184 322 607229 82.8 99.4 NP_473372 322 607229 82.8 99.4 NP_671732 322 607227 79.7 99.4 NP_001290544 330 607228 62.6 99.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9508 Download

>KIEE9508  966 bp
ATGGATCCAACCGTCCCAGTCTTCGGTACAAAACTGACACCAATCAACGGACGTGAGGAG
ACTCCTTGCTACAATCAGACCCTGAGCTTCACGGTGCTGACGTGCATCATTTCCCTTGTC
GGACTGACAGGAAACGCGGTTGTGCTCTGGCTCCTGGGCTACCGCATGCGCAGGAACGCT
GTCTCCATCTACATCCTCAACCTGGCCGCAGCAGACTTCCTCTTCCTCAGCTTCCAGATT
ATACGTTCGCCATTACGCCTCATCAATATCAGCCATCTCATCCGCAAAATCCTCGTTTCT
GTGATGACCTTTCCCTACTTTACAGGCCTGAGTATGCTGAGCGCCATCAGCACCGAGCGC
TGCCTGTCTGTTCTGTGGCCCATCTGGTACCGCTGCCGCCGCCCCACACACCTGTCAGCG
GTCGTGTGTGTCCTGCTCTGGGGCCTGTCCCTGCTGTTTAGTATGCTGGAGTGGAGGTTC
TGTGACTTCCTGTTTAGTGGTGCTGATTCTAGTTGGTGTGAAACGTCAGATTTCATCCCA
GTCGCGTGGCTGATTTTTTTATGTGTGGTTCTCTGTGTTTCCAGCCTGGTCCTGCTGGTC
AGGATCCTCTGTGGATCCCGGAAGATGCCGCTGACCAGGCTGTACGTGACCATCCTGCTC
ACAGTGCTGGTCTTCCTCCTCTGCGGCCTGCCCTTCGGCATTCTGGGGGCCCTAATTTAC
AGGATGCACCTGAATTTGGAAGTCTTATATTGTCATGTTTATCTGGTTTGCATGTCCCTG
TCCTCTCTAAACAGTAGTGCCAACCCCATCATTTACTTCTTCGTGGGCTCCTTTAGGCAG
CGTCAAAATAGGCAGAACCTGAAGCTGGTTCTCCAGAGGGCTCTGCAGGACAAGCCTGAG
GTGGATAAAGGTGAAGGGCAGCTTCCTGAGGAAAGCCTGGAGCTGTCGGGAAGCAGATTG
GGGCCA

ORF Protein

Cloned ORF protein sequence for pFN21AE9508 Download

>KIEE9508  322 aa
MDPTVPVFGTKLTPINGREETPCYNQTLSFTVLTCIISLVGLTGNAVVLWLLGYRMRRNA
VSIYILNLAAADFLFLSFQIIRSPLRLINISHLIRKILVSVMTFPYFTGLSMLSAISTER
CLSVLWPIWYRCRRPTHLSAVVCVLLWGLSLLFSMLEWRFCDFLFSGADSSWCETSDFIP
VAWLIFLCVVLCVSSLVLLVRILCGSRKMPLTRLYVTILLTVLVFLLCGLPFGILGALIY
RMHLNLEVLYCHVYLVCMSLSSLNSSANPIIYFFVGSFRQRQNRQNLKLVLQRALQDKPE
VDKGEGQLPEESLELSGSRLGP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9508 Download

>pFN21AE9508  5671 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATCCAACCGTCCCAGTCTTCGGTACAAAACT
GACACCAATCAACGGACGTGAGGAGACTCCTTGCTACAATCAGACCCTGAGCTTCACGGT
GCTGACGTGCATCATTTCCCTTGTCGGACTGACAGGAAACGCGGTTGTGCTCTGGCTCCT
GGGCTACCGCATGCGCAGGAACGCTGTCTCCATCTACATCCTCAACCTGGCCGCAGCAGA
CTTCCTCTTCCTCAGCTTCCAGATTATACGTTCGCCATTACGCCTCATCAATATCAGCCA
TCTCATCCGCAAAATCCTCGTTTCTGTGATGACCTTTCCCTACTTTACAGGCCTGAGTAT
GCTGAGCGCCATCAGCACCGAGCGCTGCCTGTCTGTTCTGTGGCCCATCTGGTACCGCTG
CCGCCGCCCCACACACCTGTCAGCGGTCGTGTGTGTCCTGCTCTGGGGCCTGTCCCTGCT
GTTTAGTATGCTGGAGTGGAGGTTCTGTGACTTCCTGTTTAGTGGTGCTGATTCTAGTTG
GTGTGAAACGTCAGATTTCATCCCAGTCGCGTGGCTGATTTTTTTATGTGTGGTTCTCTG
TGTTTCCAGCCTGGTCCTGCTGGTCAGGATCCTCTGTGGATCCCGGAAGATGCCGCTGAC
CAGGCTGTACGTGACCATCCTGCTCACAGTGCTGGTCTTCCTCCTCTGCGGCCTGCCCTT
CGGCATTCTGGGGGCCCTAATTTACAGGATGCACCTGAATTTGGAAGTCTTATATTGTCA
TGTTTATCTGGTTTGCATGTCCCTGTCCTCTCTAAACAGTAGTGCCAACCCCATCATTTA
CTTCTTCGTGGGCTCCTTTAGGCAGCGTCAAAATAGGCAGAACCTGAAGCTGGTTCTCCA
GAGGGCTCTGCAGGACAAGCCTGAGGTGGATAAAGGTGAAGGGCAGCTTCCTGAGGAAAG
CCTGGAGCTGTCGGGAAGCAGATTGGGGCCAGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT