LPAR3 ORF clone pFN21AE9566

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Halotag ORF Clone pFN21AE9566

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Clone info.

Species

human

Clone Name

pFN21AE9566 Sequence (with vector) Restriction Map

Product ID

FHC20390

ORF Sequence

DNA sequence (1059 bp)
Protein sequence (353 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9566

Symbol

LPAR3

Alias : EDG7, Edg-7, GPCR, HOFNH30, LP-A3, LPA3, RP4-678I3

Description

lysophosphatidic acid receptor 3

Original Clone ID

cp99566

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1059 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 353 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS700.1 9.3e-91 100.0 LPAR3 CCDS6777.1 1.1e-45 51.9 LPAR1 CCDS12407.1 1.1e-40 48.0 LPAR2 CCDS6680.1 1.3e-24 37.1 S1PR3 CCDS777.1 3.4e-24 35.7 S1PR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition BlastProDom NULL 315 353 PD334079 NULL FPrintScan IPR004065 4 13 PR01527 Lysophosphatidic acid receptor IPR004065 17 26 PR01527 Lysophosphatidic acid receptor IPR005385 18 30 PR01560 Lysophosphatidic acid receptor EDG-7 IPR000276 32 56 PR00237 GPCR IPR005385 35 49 PR01560 Lysophosphatidic acid receptor EDG-7 IPR000276 65 86 PR00237 GPCR IPR000276 109 131 PR00237 GPCR IPR004065 132 144 PR01527 Lysophosphatidic acid receptor IPR000276 144 165 PR00237 GPCR IPR000276 187 210 PR00237 GPCR IPR004065 214 226 PR01527 Lysophosphatidic acid receptor IPR005385 218 236 PR01560 Lysophosphatidic acid receptor EDG-7 IPR000276 237 261 PR00237 GPCR IPR004065 264 274 PR01527 Lysophosphatidic acid receptor IPR005385 265 277 PR01560 Lysophosphatidic acid receptor EDG-7 IPR000276 275 301 PR00237 GPCR IPR004065 303 314 PR01527 Lysophosphatidic acid receptor IPR005385 317 329 PR01560 Lysophosphatidic acid receptor EDG-7 IPR005385 329 341 PR01560 Lysophosphatidic acid receptor EDG-7 IPR005385 341 353 PR01560 Lysophosphatidic acid receptor EDG-7 HMMPfam IPR000276 48 293 PF00001 GPCR ProfileScan IPR017452 47 293 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 31 VIVLCVGTFFCLFIFFSNSLVIA 53 PRIMARY 23 2 66 YYLLANLAAADFFAGIAYVFLMF 88 SECONDARY 23 3 150 LLVWAIAIFMGAVPTLGWNCLCN 172 PRIMARY 23 4 190 FWTVSNLMAFLIMVVVYLRIYV 211 PRIMARY 22 5 239 KTVMTVLGAFVVCWTPGLVVLLL 261 PRIMARY 23 6 277 WFLLLALLNSVVNPIIYSY 295 SECONDARY 19
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_036284 353 605106 100.0 100.0 XP_016869893 364 602282 51.9 93.2 XP_016869890 364 602282 51.9 93.2 XP_016869889 364 602282 51.9 93.2 XP_016869883 364 602282 51.9 93.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9566 Download

>KIEE9566  1059 bp
ATGAATGAGTGTCACTATGACAAGCACATGGACTTTTTTTATAATAGGAGCAACACTGAT
ACTGTCGATGACTGGACAGGAACAAAGCTTGTGATTGTTTTGTGTGTTGGGACGTTTTTC
TGCCTGTTTATTTTTTTTTCTAATTCTCTGGTCATCGCGGCAGTGATCAAAAACAGAAAA
TTTCATTTCCCCTTCTACTACCTGTTGGCTAATTTAGCTGCTGCCGATTTCTTCGCTGGA
ATTGCCTATGTATTCCTGATGTTTAACACAGGCCCAGTTTCAAAAACTTTGACTGTCAAC
CGCTGGTTTCTCCGTCAGGGGCTTCTGGACAGTAGCTTGACTGCTTCCCTCACCAACTTG
CTGGTTATCGCCGTGGAGAGGCACATGTCAATCATGAGGATGCGGGTCCATAGCAACCTG
ACCAAAAAGAGGGTGACACTGCTCATTTTGCTTGTCTGGGCCATCGCCATTTTTATGGGG
GCGGTCCCCACACTGGGCTGGAATTGCCTCTGCAACATCTCTGCCTGCTCTTCCCTGGCC
CCCATTTACAGCAGGAGTTACCTTGTTTTCTGGACAGTGTCCAACCTCATGGCCTTCCTC
ATCATGGTTGTGGTGTACCTGCGGATCTACGTGTACGTCAAGAGGAAAACCAACGTCTTG
TCTCCGCATACAAGTGGGTCCATCAGCCGCCGGAGGACACCCATGAAGCTAATGAAGACG
GTGATGACTGTCTTAGGGGCGTTTGTGGTATGCTGGACCCCGGGCCTGGTGGTTCTGCTC
CTCGACGGCCTGAACTGCAGGCAGTGTGGCGTGCAGCATGTGAAAAGGTGGTTCCTGCTG
CTGGCGCTGCTCAACTCCGTCGTGAACCCCATCATCTACTCCTACAAGGACGAGGACATG
TATGGCACCATGAAGAAGATGATCTGCTGCTTCTCTCAGGAGAACCCAGAGAGGCGTCCC
TCTCGCATCCCCTCCACAGTCCTCAGCAGGAGTGACACAGGCAGCCAGTACATAGAGGAT
AGTATTAGCCAAGGTGCAGTCTGCAATAAAAGCACTTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE9566 Download

>KIEE9566  353 aa
MNECHYDKHMDFFYNRSNTDTVDDWTGTKLVIVLCVGTFFCLFIFFSNSLVIAAVIKNRK
FHFPFYYLLANLAAADFFAGIAYVFLMFNTGPVSKTLTVNRWFLRQGLLDSSLTASLTNL
LVIAVERHMSIMRMRVHSNLTKKRVTLLILLVWAIAIFMGAVPTLGWNCLCNISACSSLA
PIYSRSYLVFWTVSNLMAFLIMVVVYLRIYVYVKRKTNVLSPHTSGSISRRRTPMKLMKT
VMTVLGAFVVCWTPGLVVLLLDGLNCRQCGVQHVKRWFLLLALLNSVVNPIIYSYKDEDM
YGTMKKMICCFSQENPERRPSRIPSTVLSRSDTGSQYIEDSISQGAVCNKSTS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9566 Download

>pFN21AE9566  5764 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAATGAGTGTCACTATGACAAGCACATGGACTT
TTTTTATAATAGGAGCAACACTGATACTGTCGATGACTGGACAGGAACAAAGCTTGTGAT
TGTTTTGTGTGTTGGGACGTTTTTCTGCCTGTTTATTTTTTTTTCTAATTCTCTGGTCAT
CGCGGCAGTGATCAAAAACAGAAAATTTCATTTCCCCTTCTACTACCTGTTGGCTAATTT
AGCTGCTGCCGATTTCTTCGCTGGAATTGCCTATGTATTCCTGATGTTTAACACAGGCCC
AGTTTCAAAAACTTTGACTGTCAACCGCTGGTTTCTCCGTCAGGGGCTTCTGGACAGTAG
CTTGACTGCTTCCCTCACCAACTTGCTGGTTATCGCCGTGGAGAGGCACATGTCAATCAT
GAGGATGCGGGTCCATAGCAACCTGACCAAAAAGAGGGTGACACTGCTCATTTTGCTTGT
CTGGGCCATCGCCATTTTTATGGGGGCGGTCCCCACACTGGGCTGGAATTGCCTCTGCAA
CATCTCTGCCTGCTCTTCCCTGGCCCCCATTTACAGCAGGAGTTACCTTGTTTTCTGGAC
AGTGTCCAACCTCATGGCCTTCCTCATCATGGTTGTGGTGTACCTGCGGATCTACGTGTA
CGTCAAGAGGAAAACCAACGTCTTGTCTCCGCATACAAGTGGGTCCATCAGCCGCCGGAG
GACACCCATGAAGCTAATGAAGACGGTGATGACTGTCTTAGGGGCGTTTGTGGTATGCTG
GACCCCGGGCCTGGTGGTTCTGCTCCTCGACGGCCTGAACTGCAGGCAGTGTGGCGTGCA
GCATGTGAAAAGGTGGTTCCTGCTGCTGGCGCTGCTCAACTCCGTCGTGAACCCCATCAT
CTACTCCTACAAGGACGAGGACATGTATGGCACCATGAAGAAGATGATCTGCTGCTTCTC
TCAGGAGAACCCAGAGAGGCGTCCCTCTCGCATCCCCTCCACAGTCCTCAGCAGGAGTGA
CACAGGCAGCCAGTACATAGAGGATAGTATTAGCCAAGGTGCAGTCTGCAATAAAAGCAC
TTCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT