CHD2 ORF clone pFN21AE9638

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9638

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Clone info.

Species

human

Clone Name

pFN21AE9638 Sequence (with vector) Restriction Map

Product ID

FHC31523

ORF Sequence

DNA sequence (5484 bp)
Protein sequence (1828 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9638

Symbol

CHD2

Alias : DEE94, EEOC

Description

chromodomain helicase DNA binding protein 2, transcript variant 1

Original Clone ID

cp99638

Note

We obtained this clone using the E.coli strain DH5, and finally transferred it into JM109.

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 5484 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1828 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10374.2 0 100.0 CHD2 CCDS34204.1 4.8e-158 57.3 CHD1 CCDS45356.1 1e-104 100.0 CHD2 CCDS76018.1 1.2e-42 32.2 SMARCA1 CCDS32554.1 1.8e-42 33.9 CHD3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR023780 263 330 PF00385 Chromo domain IPR023780 378 447 PF00385 Chromo domain IPR000330 487 766 PF00176 SNF2-related IPR001650 826 905 PF00271 Helicase HMMSmart IPR000953 260 346 SM00298 Chromo domain/shadow IPR000953 376 449 SM00298 Chromo domain/shadow IPR014001 480 677 SM00487 DEAD-like helicase IPR001650 821 905 SM00490 Helicase ProfileScan IPR000953 261 353 PS50013 Chromo domain/shadow IPR000953 378 456 PS50013 Chromo domain/shadow IPR014001 496 666 PS51192 DEAD-like helicase IPR001650 795 946 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001262 1828 602119,615369 100.0 100.0 XP_005271923 1798 602118 56.9 99.0 XP_005271924 1710 602118 57.3 99.0 NP_001261 1710 602118 57.3 99.0 XP_011541414 1552 602118 61.9 85.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9638 Download

>KIEE9638  5484 bp
ATGATGAGAAATAAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCG
AGTCACTCAGCCTCTGAAGAAGCTTCGGGTTCAGACTCAGGCAGTCAGTCGGAAAGTGAG
CAGGGAAGTGATCCAGGAAGTGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCT
GAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCA
GAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAGCTGATGTGAAGAAGATGTGG
GAAGAATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACCATCG
CGATTTAATATTAAGGAAGAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGA
GGCCAGAGGCAGCTGAAAAAACAAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAG
GAACAAGGCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCT
GTCCCCAGAAGAACAGTGCCCAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGCGTGGA
AAGAGAAAAAAGCAAGATTCTTCTGATGAGGATGATGATGATGACGAAGCTCCCAAAAGG
CAGACTCGTCGAAGAGCGGCTAAAAACGTTAGTTACAAAGAAGATGATGACTTTGAGACT
GACTCAGATGATCTCATTGAAATGACTGGAGAAGGAGTTGATGAACAGCAAGATAATAGT
GAAACTATTGAAAAGGTCTTAGATTCAAGACTGGGAAAGAAAGGAGCCACTGGAGCATCT
ACTACTGTATATGCGATTGAAGCTAATGGCGACCCTAGTGGTGACTTTGACACTGAAAAG
GATGAAGGTGAAATCCAGTACCTCATCAAGTGGAAGGGTTGGTCTTACATCCACAGCACA
TGGGAGAGTGAAGAATCCTTACAGCAACAGAAAGTGAAGGGCCTAAAAAAACTAGAGAAC
TTCAAGAAAAAAGAGGACGAAATCAAACAATGGTTAGGGAAAGTTTCTCCTGAAGATGTA
GAATATTTCAATTGCCAACAGGAGCTGGCTTCAGAGTTGAATAAACAGTATCAGATAGTA
GAAAGAGTAATAGCTGTGAAGACAAGTAAATCTACATTGGGTCAAACAGATTTTCCAGCT
CATAGTCGGAAGCCGGCACCCTCAAATGAGCCCGAATATCTATGTAAATGGATGGGACTC
CCCTATTCAGAGTGTAGCTGGGAAGATGAAGCCCTCATTGGAAAGAAATTCCAGAATTGC
ATTGACAGCTTCCACAGTAGGAACAACTCAAAAACCATCCCAACAAGAGAATGCAAGGCC
CTGAAGCAGAGACCACGATTTGTAGCTTTAAAGAAACAACCTGCATATTTAGGAGGGGAG
AATCTGGAACTTCGAGATTATCAGCTAGAAGGTCTAAACTGGCTAGCTCATTCCTGGTGC
AAAAATAATAGTGTAATCCTTGCTGATGAAATGGGCCTAGGAAAGACCATCCAGACCATA
TCATTCCTCTCCTACCTGTTCCACCAACACCAGCTGTATGGCCCCTTTCTTATAGTCGTC
CCTTTATCCACCCTCACCTCATGGCAGAGAGAGTTTGAAATCTGGGCACCAGAGATTAAC
GTAGTGGTTTACATAGGTGACCTGATGAGCAGAAATACGATACGGGAATATGAATGGATT
CATTCCCAAACCAAAAGATTGAAGTTCAACGCACTTATAACAACATATGAGATCCTCTTG
AAAGATAAGACTGTGCTGGGCAGTATTAACTGGGCCTTTCTGGGAGTGGATGAAGCCCAT
CGGTTGAAGAATGATGACTCTTTATTGTATAAAACTCTGATTGATTTCAAGTCCAACCAT
AGGCTCCTGATTACGGGGACCCCTCTTCAGAATTCCCTCAAAGAGCTCTGGTCCTTGCTG
CACTTTATTATGCCGGAGAAGTTTGAATTTTGGGAAGATTTTGAAGAAGACCATGGGAAG
GGGAGAGAAAATGGCTACCAGAGTCTTCATAAGGTGCTAGAGCCTTTCCTTCTCCGGAGA
GTCAAAAAAGATGTGGAGAAATCCCTTCCTGCTAAAGTGGAACAGATTCTCAGGGTGGAG
ATGTCAGCCCTTCAGAAACAGTATTACAAGTGGATTCTGACCAGGAATTACAAGGCTCTT
GCCAAAGGAACAAGAGGCAGCACATCTGGTTTTCTTAATATTGTGATGGAACTGAAAAAA
TGTTGCAACCACTGCTATCTGATTAAACCCCCTGAAGAAAATGAAAGGGAAAATGGACAG
GAGATTCTTCTGTCCCTCATAAGGAGCAGTGGGAAGTTGATTTTATTAGACAAACTGTTG
ACAAGACTTCGAGAAAGGGGGAATCGAGTGCTTATCTTCTCTCAGATGGTGAGAATGTTG
GATATCCTGGCTGAATACCTAACTATTAAACACTATCCTTTCCAGCGTCTGGATGGTTCC
ATCAAGGGAGAAATCCGAAAACAGGCACTGGACCACTTCAATGCAGATGGGTCTGAGGAC
TTCTGTTTCCTGCTCTCGACAAGGGCTGGTGGCCTGGGAATCAATTTGGCTTCAGCGGAC
ACAGTCGTCATCTTTGACTCTGACTGGAACCCCCAGAATGACTTGCAGGCACAAGCCCGA
GCGCATAGAATTGGTCAAAAGAAGCAGGTAAATATTTACCGCTTAGTTACAAAGGGGACT
GTGGAGGAGGAGATCATAGAACGGGCCAAAAAGAAGATGGTATTAGATCATCTGGTGATT
CAGCGCATGGACACCACTGGCCGGACGATCCTGGAAAACAACTCAGGAAGGTCCAACTCA
AATCCTTTTAATAAAGAAGAGCTGACAGCTATTTTGAAATTTGGAGCAGAGGATCTCTTC
AAAGAACTGGAAGGGGAGGAATCAGAACCTCAGGAAATGGATATAGATGAAATTTTGCGG
TTGGCTGAAACGAGAGAGAATGAAGTGTCAACAAGTGCAACAGATGAACTTCTATCACAG
TTTAAGGTTGCCAACTTTGCAACAATGGAAGATGAAGAAGAGCTAGAAGAGCGTCCTCAC
AAGGACTGGGATGAGATCATTCCAGAGGAACAAAGGAAAAAAGTAGAGGAGGAAGAGCGG
CAGAAGGAGCTAGAAGAAATTTATATGCTGCCTCGAATTCGGAGTTCCACTAAAAAGGCT
CAGACAAATGACAGTGACTCTGACACTGAGTCTAAGAGGCAGGCCCAGAGATCCTCTGCT
TCTGAGAGTGAAACGGAAGACTCTGATGATGACAAGAAGCCAAAGCGCAGAGGGCGTCCG
AGGAGTGTGCGGAAGGACCTCGTGGAGGGATTTACTGATGCAGAGATCCGAAGGTTCATC
AAGGCTTATAAGAAGTTTGGTCTCCCTCTTGAACGGCTGGAGTGCATAGCACGTGATGCT
GAGCTGGTAGATAAGTCGGTGGCAGATCTGAAGCGCCTGGGTGAACTGATCCACAACAGC
TGTGTGTCAGCAATGCAGGAATACGAAGAGCAGCTGAAAGAAAATGCCAGCGAGGGAAAA
GGACCAGGGAAAAGGAGAGGTCCAACAATCAAGATATCCGGAGTTCAGGTTAATGTGAAA
TCCATTATCCAACATGAAGAGGAGTTTGAGATGCTGCATAAATCTATCCCTGTGGACCCT
GAAGAAAAAAAAAAATACTGCTTAACCTGTCGTGTCAAAGCTGCACATTTTGATGTAGAG
TGGGGGGTGGAAGATGATTCTCGCCTGTTGCTGGGGATTTATGAACATGGCTATGGAAAC
TGGGAGTTAATTAAAACAGACCCAGAGCTTAAATTAACTGACAAAATTCTGCCGGTGGAG
ACAGATAAAAAGCCTCAGGGGAAGCAGCTACAGACCCGAGCGGATTACTTGTTGAAGCTG
CTCAGAAAGGGTCTGGAGAAGAAGGGGGCTGTGACAGGTGGGGAAGAGGCCAAATTAAAG
AAGCGGAAGCCTCGGGTAAAGAAGGAAAACAAAGTGCCCAGGCTGAAAGAGGAGCATGGA
ATTGAGCTTTCATCTCCTAGGCATTCAGATAATCCATCAGAAGAGGGAGAAGTGAAAGAT
GATGGCTTGGAAAAAAGTCCAATGAAAAAAAAACAGAAGAAGAAAGAGAACAAGGAGAAC
AAGGAGAAACAAATGAGTTCTAGGAAAGACAAAGAAGGGGACAAGGAAAGAAAGAAGTCA
AAAGATAAGAAAGAGAAGCCTAAAAGTGGTGATGCCAAATCTTCGAGTAAATCAAAGCGA
TCTCAGGGTCCTGTCCATATTACAGCAGGAAGTGAACCTGTCCCCATTGGAGAGGATGAG
GATGATGATCTGGACCAGGAGACATTCAGCATATGTAAGGAGAGGATGAGGCCCGTGAAA
AAGGCACTGAAACAGCTCGACAAACCTGACAAGGGGCTCAACGTGCAAGAACAGCTGGAA
CACACCCGGAACTGCCTGCTGAAAATCGGAGACCGGATAGCCGAGTGCCTTAAAGCCTAC
TCAGATCAGGAGCACATCAAACTCTGGAGGAGGAACCTATGGATTTTTGTTTCCAAGTTT
ACAGAATTTGATGCTCGAAAACTGCATAAGTTATACAAGATGGCTCATAAGAAAAGGTCT
CAAGAAGAAGAGGAGCAAAAGAAGAAAGACGACGTGACTGGGGGTAAGAAACCATTTCGT
CCAGAGGCCTCAGGCTCCAGCCGGGACTCTCTGATATCTCAGTCCCATACCTCACACAAC
CTTCACCCTCAGAAGCCTCATTTGCCTGCCTCCCATGGCCCACAGATGCATGGACACCCA
AGAGATAACTACAATCACCCCAACAAGAGACACTTCAGTAATGCAGATCGAGGAGACTGG
CAGAGGGAAAGAAAGTTCAACTATGGTGGTGGCAACAACAATCCACCATGGGGAAGCGAC
AGGCACCATCAGTATGAGCAGCACTGGTACAAGGACCACCATTATGGGGATCGGCGACAT
ATGGATGCCCACCGTTCCGGAAGCTATCGACCCAACAACATGTCCAGAAAGAGGCCTTAT
GACCAGTACAGCAGTGACCGAGACCACCGGGGACACAGAGATTATTATGACAGGCACCAT
CATGACTCCAAGCGGAGGAGATCCGATGAATTTAGGCCTCAAAATTACCACCAGCAGGAT
TTCCGACGAATGTCTGATCACCGCCCCGCTATGGGCTACCATGGCCAGGGACCCTCAGAC
CATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCCATTG
CACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCACGATTCCAAG
TCACCCCTGGATCATAGGTCTCCTTTGGAGAGATCACTAGAACAGAAAAACAACCCAGAT
TATAACTGGAATGTTCGGAAAACA

ORF Protein

Cloned ORF protein sequence for pFN21AE9638 Download

>KIEE9638  1828 aa
MMRNKDKSQEEDSSLHSNASSHSASEEASGSDSGSQSESEQGSDPGSGHGSESNSSSESS
ESQSESESESAGSKSQPVLPEAKEKPASKKERIADVKKMWEEYPDVYGVRRSNRSRQEPS
RFNIKEEASSGSESGSPKRRGQRQLKKQEKWKQEPSEDEQEQGTSAESEPEQKKVKARRP
VPRRTVPKPRVKKQPKTQRGKRKKQDSSDEDDDDDEAPKRQTRRRAAKNVSYKEDDDFET
DSDDLIEMTGEGVDEQQDNSETIEKVLDSRLGKKGATGASTTVYAIEANGDPSGDFDTEK
DEGEIQYLIKWKGWSYIHSTWESEESLQQQKVKGLKKLENFKKKEDEIKQWLGKVSPEDV
EYFNCQQELASELNKQYQIVERVIAVKTSKSTLGQTDFPAHSRKPAPSNEPEYLCKWMGL
PYSECSWEDEALIGKKFQNCIDSFHSRNNSKTIPTRECKALKQRPRFVALKKQPAYLGGE
NLELRDYQLEGLNWLAHSWCKNNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFLIVV
PLSTLTSWQREFEIWAPEINVVVYIGDLMSRNTIREYEWIHSQTKRLKFNALITTYEILL
KDKTVLGSINWAFLGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLL
HFIMPEKFEFWEDFEEDHGKGRENGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVE
MSALQKQYYKWILTRNYKALAKGTRGSTSGFLNIVMELKKCCNHCYLIKPPEENERENGQ
EILLSLIRSSGKLILLDKLLTRLRERGNRVLIFSQMVRMLDILAEYLTIKHYPFQRLDGS
IKGEIRKQALDHFNADGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQAR
AHRIGQKKQVNIYRLVTKGTVEEEIIERAKKKMVLDHLVIQRMDTTGRTILENNSGRSNS
NPFNKEELTAILKFGAEDLFKELEGEESEPQEMDIDEILRLAETRENEVSTSATDELLSQ
FKVANFATMEDEEELEERPHKDWDEIIPEEQRKKVEEEERQKELEEIYMLPRIRSSTKKA
QTNDSDSDTESKRQAQRSSASESETEDSDDDKKPKRRGRPRSVRKDLVEGFTDAEIRRFI
KAYKKFGLPLERLECIARDAELVDKSVADLKRLGELIHNSCVSAMQEYEEQLKENASEGK
GPGKRRGPTIKISGVQVNVKSIIQHEEEFEMLHKSIPVDPEEKKKYCLTCRVKAAHFDVE
WGVEDDSRLLLGIYEHGYGNWELIKTDPELKLTDKILPVETDKKPQGKQLQTRADYLLKL
LRKGLEKKGAVTGGEEAKLKKRKPRVKKENKVPRLKEEHGIELSSPRHSDNPSEEGEVKD
DGLEKSPMKKKQKKKENKENKEKQMSSRKDKEGDKERKKSKDKKEKPKSGDAKSSSKSKR
SQGPVHITAGSEPVPIGEDEDDDLDQETFSICKERMRPVKKALKQLDKPDKGLNVQEQLE
HTRNCLLKIGDRIAECLKAYSDQEHIKLWRRNLWIFVSKFTEFDARKLHKLYKMAHKKRS
QEEEEQKKKDDVTGGKKPFRPEASGSSRDSLISQSHTSHNLHPQKPHLPASHGPQMHGHP
RDNYNHPNKRHFSNADRGDWQRERKFNYGGGNNNPPWGSDRHHQYEQHWYKDHHYGDRRH
MDAHRSGSYRPNNMSRKRPYDQYSSDRDHRGHRDYYDRHHHDSKRRRSDEFRPQNYHQQD
FRRMSDHRPAMGYHGQGPSDHYRSFHTDKLGEYKQPLPPLHPAVSDPRSPPSQKSPHDSK
SPLDHRSPLERSLEQKNNPDYNWNVRKT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9638 Download

>pFN21AE9638  10189 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATGAGAAATAAGGACAAAAGCCAAGAGGAGGA
CAGTTCGCTACACAGCAATGCATCGAGTCACTCAGCCTCTGAAGAAGCTTCGGGTTCAGA
CTCAGGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAGTGGACATGGCAGCGA
GTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGG
TTCCAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACG
GATAGCTGATGTGAAGAAGATGTGGGAAGAATATCCTGATGTTTATGGGGTCAGGCGGTC
AAACCGAAGCAGACAAGAACCATCGCGATTTAATATTAAGGAAGAGGCAAGTAGCGGGTC
TGAGAGTGGGAGCCCAAAAAGAAGAGGCCAGAGGCAGCTGAAAAAACAAGAAAAATGGAA
ACAGGAACCCTCAGAAGATGAACAGGAACAAGGCACCAGTGCAGAGAGTGAGCCAGAACA
AAAAAAAGTAAAAGCCAGAAGACCTGTCCCCAGAAGAACAGTGCCCAAACCTCGTGTTAA
AAAGCAGCCGAAGACTCAGCGTGGAAAGAGAAAAAAGCAAGATTCTTCTGATGAGGATGA
TGATGATGACGAAGCTCCCAAAAGGCAGACTCGTCGAAGAGCGGCTAAAAACGTTAGTTA
CAAAGAAGATGATGACTTTGAGACTGACTCAGATGATCTCATTGAAATGACTGGAGAAGG
AGTTGATGAACAGCAAGATAATAGTGAAACTATTGAAAAGGTCTTAGATTCAAGACTGGG
AAAGAAAGGAGCCACTGGAGCATCTACTACTGTATATGCGATTGAAGCTAATGGCGACCC
TAGTGGTGACTTTGACACTGAAAAGGATGAAGGTGAAATCCAGTACCTCATCAAGTGGAA
GGGTTGGTCTTACATCCACAGCACATGGGAGAGTGAAGAATCCTTACAGCAACAGAAAGT
GAAGGGCCTAAAAAAACTAGAGAACTTCAAGAAAAAAGAGGACGAAATCAAACAATGGTT
AGGGAAAGTTTCTCCTGAAGATGTAGAATATTTCAATTGCCAACAGGAGCTGGCTTCAGA
GTTGAATAAACAGTATCAGATAGTAGAAAGAGTAATAGCTGTGAAGACAAGTAAATCTAC
ATTGGGTCAAACAGATTTTCCAGCTCATAGTCGGAAGCCGGCACCCTCAAATGAGCCCGA
ATATCTATGTAAATGGATGGGACTCCCCTATTCAGAGTGTAGCTGGGAAGATGAAGCCCT
CATTGGAAAGAAATTCCAGAATTGCATTGACAGCTTCCACAGTAGGAACAACTCAAAAAC
CATCCCAACAAGAGAATGCAAGGCCCTGAAGCAGAGACCACGATTTGTAGCTTTAAAGAA
ACAACCTGCATATTTAGGAGGGGAGAATCTGGAACTTCGAGATTATCAGCTAGAAGGTCT
AAACTGGCTAGCTCATTCCTGGTGCAAAAATAATAGTGTAATCCTTGCTGATGAAATGGG
CCTAGGAAAGACCATCCAGACCATATCATTCCTCTCCTACCTGTTCCACCAACACCAGCT
GTATGGCCCCTTTCTTATAGTCGTCCCTTTATCCACCCTCACCTCATGGCAGAGAGAGTT
TGAAATCTGGGCACCAGAGATTAACGTAGTGGTTTACATAGGTGACCTGATGAGCAGAAA
TACGATACGGGAATATGAATGGATTCATTCCCAAACCAAAAGATTGAAGTTCAACGCACT
TATAACAACATATGAGATCCTCTTGAAAGATAAGACTGTGCTGGGCAGTATTAACTGGGC
CTTTCTGGGAGTGGATGAAGCCCATCGGTTGAAGAATGATGACTCTTTATTGTATAAAAC
TCTGATTGATTTCAAGTCCAACCATAGGCTCCTGATTACGGGGACCCCTCTTCAGAATTC
CCTCAAAGAGCTCTGGTCCTTGCTGCACTTTATTATGCCGGAGAAGTTTGAATTTTGGGA
AGATTTTGAAGAAGACCATGGGAAGGGGAGAGAAAATGGCTACCAGAGTCTTCATAAGGT
GCTAGAGCCTTTCCTTCTCCGGAGAGTCAAAAAAGATGTGGAGAAATCCCTTCCTGCTAA
AGTGGAACAGATTCTCAGGGTGGAGATGTCAGCCCTTCAGAAACAGTATTACAAGTGGAT
TCTGACCAGGAATTACAAGGCTCTTGCCAAAGGAACAAGAGGCAGCACATCTGGTTTTCT
TAATATTGTGATGGAACTGAAAAAATGTTGCAACCACTGCTATCTGATTAAACCCCCTGA
AGAAAATGAAAGGGAAAATGGACAGGAGATTCTTCTGTCCCTCATAAGGAGCAGTGGGAA
GTTGATTTTATTAGACAAACTGTTGACAAGACTTCGAGAAAGGGGGAATCGAGTGCTTAT
CTTCTCTCAGATGGTGAGAATGTTGGATATCCTGGCTGAATACCTAACTATTAAACACTA
TCCTTTCCAGCGTCTGGATGGTTCCATCAAGGGAGAAATCCGAAAACAGGCACTGGACCA
CTTCAATGCAGATGGGTCTGAGGACTTCTGTTTCCTGCTCTCGACAAGGGCTGGTGGCCT
GGGAATCAATTTGGCTTCAGCGGACACAGTCGTCATCTTTGACTCTGACTGGAACCCCCA
GAATGACTTGCAGGCACAAGCCCGAGCGCATAGAATTGGTCAAAAGAAGCAGGTAAATAT
TTACCGCTTAGTTACAAAGGGGACTGTGGAGGAGGAGATCATAGAACGGGCCAAAAAGAA
GATGGTATTAGATCATCTGGTGATTCAGCGCATGGACACCACTGGCCGGACGATCCTGGA
AAACAACTCAGGAAGGTCCAACTCAAATCCTTTTAATAAAGAAGAGCTGACAGCTATTTT
GAAATTTGGAGCAGAGGATCTCTTCAAAGAACTGGAAGGGGAGGAATCAGAACCTCAGGA
AATGGATATAGATGAAATTTTGCGGTTGGCTGAAACGAGAGAGAATGAAGTGTCAACAAG
TGCAACAGATGAACTTCTATCACAGTTTAAGGTTGCCAACTTTGCAACAATGGAAGATGA
AGAAGAGCTAGAAGAGCGTCCTCACAAGGACTGGGATGAGATCATTCCAGAGGAACAAAG
GAAAAAAGTAGAGGAGGAAGAGCGGCAGAAGGAGCTAGAAGAAATTTATATGCTGCCTCG
AATTCGGAGTTCCACTAAAAAGGCTCAGACAAATGACAGTGACTCTGACACTGAGTCTAA
GAGGCAGGCCCAGAGATCCTCTGCTTCTGAGAGTGAAACGGAAGACTCTGATGATGACAA
GAAGCCAAAGCGCAGAGGGCGTCCGAGGAGTGTGCGGAAGGACCTCGTGGAGGGATTTAC
TGATGCAGAGATCCGAAGGTTCATCAAGGCTTATAAGAAGTTTGGTCTCCCTCTTGAACG
GCTGGAGTGCATAGCACGTGATGCTGAGCTGGTAGATAAGTCGGTGGCAGATCTGAAGCG
CCTGGGTGAACTGATCCACAACAGCTGTGTGTCAGCAATGCAGGAATACGAAGAGCAGCT
GAAAGAAAATGCCAGCGAGGGAAAAGGACCAGGGAAAAGGAGAGGTCCAACAATCAAGAT
ATCCGGAGTTCAGGTTAATGTGAAATCCATTATCCAACATGAAGAGGAGTTTGAGATGCT
GCATAAATCTATCCCTGTGGACCCTGAAGAAAAAAAAAAATACTGCTTAACCTGTCGTGT
CAAAGCTGCACATTTTGATGTAGAGTGGGGGGTGGAAGATGATTCTCGCCTGTTGCTGGG
GATTTATGAACATGGCTATGGAAACTGGGAGTTAATTAAAACAGACCCAGAGCTTAAATT
AACTGACAAAATTCTGCCGGTGGAGACAGATAAAAAGCCTCAGGGGAAGCAGCTACAGAC
CCGAGCGGATTACTTGTTGAAGCTGCTCAGAAAGGGTCTGGAGAAGAAGGGGGCTGTGAC
AGGTGGGGAAGAGGCCAAATTAAAGAAGCGGAAGCCTCGGGTAAAGAAGGAAAACAAAGT
GCCCAGGCTGAAAGAGGAGCATGGAATTGAGCTTTCATCTCCTAGGCATTCAGATAATCC
ATCAGAAGAGGGAGAAGTGAAAGATGATGGCTTGGAAAAAAGTCCAATGAAAAAAAAACA
GAAGAAGAAAGAGAACAAGGAGAACAAGGAGAAACAAATGAGTTCTAGGAAAGACAAAGA
AGGGGACAAGGAAAGAAAGAAGTCAAAAGATAAGAAAGAGAAGCCTAAAAGTGGTGATGC
CAAATCTTCGAGTAAATCAAAGCGATCTCAGGGTCCTGTCCATATTACAGCAGGAAGTGA
ACCTGTCCCCATTGGAGAGGATGAGGATGATGATCTGGACCAGGAGACATTCAGCATATG
TAAGGAGAGGATGAGGCCCGTGAAAAAGGCACTGAAACAGCTCGACAAACCTGACAAGGG
GCTCAACGTGCAAGAACAGCTGGAACACACCCGGAACTGCCTGCTGAAAATCGGAGACCG
GATAGCCGAGTGCCTTAAAGCCTACTCAGATCAGGAGCACATCAAACTCTGGAGGAGGAA
CCTATGGATTTTTGTTTCCAAGTTTACAGAATTTGATGCTCGAAAACTGCATAAGTTATA
CAAGATGGCTCATAAGAAAAGGTCTCAAGAAGAAGAGGAGCAAAAGAAGAAAGACGACGT
GACTGGGGGTAAGAAACCATTTCGTCCAGAGGCCTCAGGCTCCAGCCGGGACTCTCTGAT
ATCTCAGTCCCATACCTCACACAACCTTCACCCTCAGAAGCCTCATTTGCCTGCCTCCCA
TGGCCCACAGATGCATGGACACCCAAGAGATAACTACAATCACCCCAACAAGAGACACTT
CAGTAATGCAGATCGAGGAGACTGGCAGAGGGAAAGAAAGTTCAACTATGGTGGTGGCAA
CAACAATCCACCATGGGGAAGCGACAGGCACCATCAGTATGAGCAGCACTGGTACAAGGA
CCACCATTATGGGGATCGGCGACATATGGATGCCCACCGTTCCGGAAGCTATCGACCCAA
CAACATGTCCAGAAAGAGGCCTTATGACCAGTACAGCAGTGACCGAGACCACCGGGGACA
CAGAGATTATTATGACAGGCACCATCATGACTCCAAGCGGAGGAGATCCGATGAATTTAG
GCCTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCCGCTATGGG
CTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCCACACAGATAAACTGGGGGA
ATATAAACAGCCTCTACCCCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTC
TCAGAAATCTCCTCACGATTCCAAGTCACCCCTGGATCATAGGTCTCCTTTGGAGAGATC
ACTAGAACAGAAAAACAACCCAGATTATAACTGGAATGTTCGGAAAACAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT