SACM1L ORF clone pFN21ASDA0851

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21ASDA0851

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Clone info.

Species

human

Clone Name

pFN21ASDA0851 Sequence (with vector) Restriction Map

Product ID

FHC00658

Flexi Clone PID

FXC00658

ORF Sequence

DNA sequence (1761 bp)
Protein sequence (587 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384015]

Gene

KIAA0851

Symbol

SACM1L

Alias : SAC1

Description

SAC1 suppressor of actin mutations 1-like (yeast)

Original Clone ID

hk06032

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1761 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 587 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33745.1 0 100.0 SACM1L CCDS82762.1 0 100.0 SACM1L CCDS7616.1 6.3e-57 38.0 INPP5F CCDS54484.1 3e-38 31.3 SYNJ1 CCDS54483.1 3.7e-38 31.2 SYNJ1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002013 57 350 PF02383 Synaptojanin ProfileScan IPR002013 122 451 PS50275 Synaptojanin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 521 FLALPIIMVVAFSMCIICLLMAG 543 PRIMARY 23 2 554 LFWGVASIGTFFIILYNGKDFVD 576 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_054735 587 606569 100.0 100.0 NP_001306002 484 606569 100.0 82.5 NP_001306001 526 606569 100.0 81.3 XP_011531802 526 606569 100.0 81.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0851 Download

>KIAA0851  1761 bp
ATGGCGACGGCGGCCTACGAGCAGCTGAAGCTGCATATCACACCTGAAAAATTTTATGTG
GAAGCTTGTGATGATGGAGCAGATGACGTACTTACCATTGACCGTGTGTCCACAGAGGTT
ACCCTTGCAGTCAAGAAAGATGTTCCTCCTTCAGCTGTCACAAGACCAATATTTGGTATA
CTGGGCACAATCCATCTGGTGGCAGGTAATTATCTTATAGTCATTACCAAAAAGATAAAA
GTAGGTGAATTTTTCAGTCATGTAGTCTGGAAAGCAACAGATTTTGATGTCCTTTCTTAT
AAGAAGACAATGTTGCACTTAACTGATATTCAGTTACAAGATAATAAAACCTTCCTAGCG
ATGCTAAACCATGTCTTGAATGTGGATGGATTTTACTTTTCAACAACATATGATTTGACC
CATACTTTGCAGCGGCTATCCAACACTAGTCCTGAATTCCAAGAAATGAGTCTCTTGGAA
AGGGCAGATCAGCGGTTTGTATGGAATGGTCATCTTCTAAGAGAACTTTCTGCACAGCCA
GAGGTTCATCGGTTTGCCCTTCCAGTGTTACATGGCTTTATTACCATGCATTCATGTTCT
ATTAATGGAAAATACTTTGATTGGATTCTCATCTCGAGGAGGAGCTGTTTCAGAGCTGGT
GTGCGCTATTATGTAAGAGGAATTGATTCGGAAGGCCATGCAGCTAACTTTGTAGAAACA
GAACAAATTGTGCACTACAATGGGAGCAAAGCTTCGTTTGTACAGACTCGAGGATCAATA
CCTGTTTTCTGGTCCCAAAGACCAAACCTCAAGTACAAACCACTGCCACAGATCAGCAAA
GTAGCAAATCACATGGACGGTTTCCAAAGGCATTTTGATTCCCAAGTAATTATTTATGGA
AAACAAGTTATAATCAATCTGATTAACCAGAAGGGCTCGGAGAAGCCACTTGAGCAGACA
TTTGCAACAATGGTGTCTTCCTTGGGAAGTGGAATGATGAGATACATTGCCTTTGACTTC
CATAAGGAATGTAAAAATATGAGATGGGATCGACTAAGTATTTTATTGGATCAGGTAGCA
GAAATGCAAGATGAATTAAGTTATTTTCTAGTGGACTCTGCTGGCCAGGTGGTGGCAAAC
CAGGAAGGCGTGTTCCGAAGCAATTGCATGGATTGTCTAGATAGAACCAATGTGATCCAG
AGTTTGTTAGCTCGTCGTTCACTTCAGGCCCAACTTCAGAGACTAGGAGTTTTGCATGTG
GGACAAAAGCTTGAAGAACAAGATGAATTTGAGAAGATTTACAAAAATGCCTGGGCTGAC
AACGCAAATGCTTGTGCCAAGCAATATGCGGGAACTGGTGCCTTGAAGACTGACTTTACC
AGAACTGGAAAGAGAACTCATTTGGGACTTATAATGGATGGCTGGAACTCAATGATACGA
TATTATAAGAACAACTTTTCCGATGGATTTAGACAAGATTCCATAGACTTATTTCTTGGA
AACTATTCAGTGGATGAATTAGAATCTCATAGTCCTTTAAGTGTTCCAAGGGACTGGAAA
TTCCTGGCTTTGCCTATTATCATGGTTGTTGCCTTTTCAATGTGCATTATCTGTTTGCTT
ATGGCTGGTGACACTTGGACAGAAACACTGGCCTATGTGCTCTTCTGGGGAGTTGCAAGC
ATTGGAACATTTTTTATCATTCTTTACAATGGCAAAGATTTTGTCGATGCTCCCAGACTG
GTCCAGAAAGAAAAGATAGAC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0851 Download

>KIAA0851  587 aa
MATAAYEQLKLHITPEKFYVEACDDGADDVLTIDRVSTEVTLAVKKDVPPSAVTRPIFGI
LGTIHLVAGNYLIVITKKIKVGEFFSHVVWKATDFDVLSYKKTMLHLTDIQLQDNKTFLA
MLNHVLNVDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQP
EVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVET
EQIVHYNGSKASFVQTRGSIPVFWSQRPNLKYKPLPQISKVANHMDGFQRHFDSQVIIYG
KQVIINLINQKGSEKPLEQTFATMVSSLGSGMMRYIAFDFHKECKNMRWDRLSILLDQVA
EMQDELSYFLVDSAGQVVANQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHV
GQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTHLGLIMDGWNSMIR
YYKNNFSDGFRQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLL
MAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0851 Download

>pFN21ASDA0851  6490 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCGACGGCGGCCTA
CGAGCAGCTGAAGCTGCATATCACACCTGAAAAATTTTATGTGGAAGCTTGTGATGATGG
AGCAGATGACGTACTTACCATTGACCGTGTGTCCACAGAGGTTACCCTTGCAGTCAAGAA
AGATGTTCCTCCTTCAGCTGTCACAAGACCAATATTTGGTATACTGGGCACAATCCATCT
GGTGGCAGGTAATTATCTTATAGTCATTACCAAAAAGATAAAAGTAGGTGAATTTTTCAG
TCATGTAGTCTGGAAAGCAACAGATTTTGATGTCCTTTCTTATAAGAAGACAATGTTGCA
CTTAACTGATATTCAGTTACAAGATAATAAAACCTTCCTAGCGATGCTAAACCATGTCTT
GAATGTGGATGGATTTTACTTTTCAACAACATATGATTTGACCCATACTTTGCAGCGGCT
ATCCAACACTAGTCCTGAATTCCAAGAAATGAGTCTCTTGGAAAGGGCAGATCAGCGGTT
TGTATGGAATGGTCATCTTCTAAGAGAACTTTCTGCACAGCCAGAGGTTCATCGGTTTGC
CCTTCCAGTGTTACATGGCTTTATTACCATGCATTCATGTTCTATTAATGGAAAATACTT
TGATTGGATTCTCATCTCGAGGAGGAGCTGTTTCAGAGCTGGTGTGCGCTATTATGTAAG
AGGAATTGATTCGGAAGGCCATGCAGCTAACTTTGTAGAAACAGAACAAATTGTGCACTA
CAATGGGAGCAAAGCTTCGTTTGTACAGACTCGAGGATCAATACCTGTTTTCTGGTCCCA
AAGACCAAACCTCAAGTACAAACCACTGCCACAGATCAGCAAAGTAGCAAATCACATGGA
CGGTTTCCAAAGGCATTTTGATTCCCAAGTAATTATTTATGGAAAACAAGTTATAATCAA
TCTGATTAACCAGAAGGGCTCGGAGAAGCCACTTGAGCAGACATTTGCAACAATGGTGTC
TTCCTTGGGAAGTGGAATGATGAGATACATTGCCTTTGACTTCCATAAGGAATGTAAAAA
TATGAGATGGGATCGACTAAGTATTTTATTGGATCAGGTAGCAGAAATGCAAGATGAATT
AAGTTATTTTCTAGTGGACTCTGCTGGCCAGGTGGTGGCAAACCAGGAAGGCGTGTTCCG
AAGCAATTGCATGGATTGTCTAGATAGAACCAATGTGATCCAGAGTTTGTTAGCTCGTCG
TTCACTTCAGGCCCAACTTCAGAGACTAGGAGTTTTGCATGTGGGACAAAAGCTTGAAGA
ACAAGATGAATTTGAGAAGATTTACAAAAATGCCTGGGCTGACAACGCAAATGCTTGTGC
CAAGCAATATGCGGGAACTGGTGCCTTGAAGACTGACTTTACCAGAACTGGAAAGAGAAC
TCATTTGGGACTTATAATGGATGGCTGGAACTCAATGATACGATATTATAAGAACAACTT
TTCCGATGGATTTAGACAAGATTCCATAGACTTATTTCTTGGAAACTATTCAGTGGATGA
ATTAGAATCTCATAGTCCTTTAAGTGTTCCAAGGGACTGGAAATTCCTGGCTTTGCCTAT
TATCATGGTTGTTGCCTTTTCAATGTGCATTATCTGTTTGCTTATGGCTGGTGACACTTG
GACAGAAACACTGGCCTATGTGCTCTTCTGGGGAGTTGCAAGCATTGGAACATTTTTTAT
CATTCTTTACAATGGCAAAGATTTTGTCGATGCTCCCAGACTGGTCCAGAAAGAAAAGAT
AGACTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT