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Order Kazusa clone(s) from : |
| Product ID | ORK00059 |
|---|---|
| Accession No | AB002356 |
| Description | MAP-kinase activating death domain, transcript variant 7 |
| Clone name | hh00017 |
| Vector information | |
| cDNA sequence | DNA sequence (5942 bp) Predicted protein sequence (1584 aa) |
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HaloTag ORF Clone |
FHC00059
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| Flexi ORF Clone | FXC00059 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0358
by Kazusa Mouse cDNA Project
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Length: 5942 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 1584 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR005113 | 11 | 101 | PF03456 | uDENN |
| IPR001194 | 175 | 405 | PF02141 | DENN | |
| IPR005112 | 488 | 561 | PF03455 | dDENN | |
| HMMSmart | IPR005113 | 11 | 101 | SM00800 | uDENN |
| IPR001194 | 175 | 405 | SM00799 | DENN | |
| IPR005112 | 488 | 558 | SM00801 | dDENN | |
| ProfileScan | IPR005113 | 11 | 107 | PS50946 | uDENN |
| IPR001194 | 175 | 405 | PS50211 | DENN | |
| IPR005112 | 488 | 561 | PS50947 | dDENN |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 303 | PLELLGVDACLQVLTCILLEHKV | 325 | PRIMARY | 23 | 2 | 336 | SMSVMAFVAMIYPLEYMFPVIPL | 358 | PRIMARY | 23 | 3 | 369 | LLLAPTPYIIGVPASFFLYKLDF | 391 | SECONDARY | 23 |
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RT-PCR
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Experimental conditions| Primer_f | GAATGCTGACTCCTTGCTTGG |
|---|---|
| Primer_r | TCAAGTAACCGAGGACAGAAC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 11
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GAATGCTGACTCCTTGCTTGG |
| Primer_r | TCAAGTAACCGAGGACAGAAC |
| PCR product length | 100 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |