|
Order Kazusa clone(s) from : |
| Product ID | ORK00599 |
|---|---|
| Accession No | AB014566 |
| Description | dishevelled associated activator of morphogenesis 1, transcript variant 1 |
| Clone name | hk02075 |
| Vector information | |
| cDNA sequence | DNA sequence (4153 bp) Predicted protein sequence (1085 aa) |
|
HaloTag ORF Clone |
FHC00599
|
| Flexi ORF Clone | FXC00599 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0666
by Kazusa Mouse cDNA Project
|
Length: 4153 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Length: 1085 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR010473 | 52 | 240 | PF06371 | Diaphanous GTPase-binding |
| IPR010472 | 242 | 448 | PF06367 | Diaphanous FH3 | |
| IPR015425 | 608 | 991 | PF02181 | Actin-binding FH2 | |
| HMMSmart | IPR003104 | 607 | 1078 | SM00498 | Actin-binding FH2 and DRF autoregulatory |
| ProfileScan | IPR014768 | 52 | 427 | PS51232 | GTPase-binding/formin homology 3 |
| IPR014767 | 1034 | 1065 | PS51231 | Diaphanous autoregulatory |
RT-PCR
|
|---|
RT-PCR-ELISA
|
Experimental conditions| Primer_f | CACATCAGTAATAGGACCAGC |
|---|---|
| Primer_r | GCACTTGGCAGAGATAACTTG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 14
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CACATCAGTAATAGGACCAGC |
| Primer_r | GCACTTGGCAGAGATAACTTG |
| PCR product length | 184 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |