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Order Kazusa clone(s) from : |
| Product ID | ORK05925 |
|---|---|
| Accession No | AB014587 |
| Description | mitogen-activated protein kinase kinase kinase kinase 4 |
| Clone name | hk02992 |
| Vector information | |
| cDNA sequence | DNA sequence (4266 bp) Predicted protein sequence (1175 aa) |
| Source | Human adult brain |
| Rouge ID |
mKIAA0687
by Kazusa Mouse cDNA Project
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Length: 4266 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Length: 1175 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| BlastProDom | IPR000719 | 39 | 251 | PD000001 | Protein kinase |
| HMMPfam | IPR000719 | 1 | 252 | PF00069 | Protein kinase |
| IPR001180 | 862 | 1153 | PF00780 | Citron-like | |
| HMMSmart | IPR001245 | 1 | 252 | SM00219 | Tyrosine protein kinase |
| IPR002290 | 1 | 252 | SM00220 | Serine/threonine protein kinase | |
| IPR001180 | 857 | 1155 | SM00036 | Citron-like | |
| ProfileScan | IPR000719 | 1 | 252 | PS50011 | Protein kinase |
| ScanRegExp | IPR008271 | 112 | 124 | PS00108 | Serine/threonine protein kinase |
RT-PCR
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RT-PCR-ELISA
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Experimental conditions| Primer_f | GTAGAAGCAGTGTGATCCAGG |
|---|---|
| Primer_r | CCCAACACATGAGGTCTGCAC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 2
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GTAGAAGCAGTGTGATCCAGG |
| Primer_r | CCCAACACATGAGGTCTGCAC |
| PCR product length | 138 bp |
| PCR conditions | 95 °C 15 sec 66 °C 60 sec 30 cycles |