KIAA0822

Product ID	ORK04008
Accession No	AB020629
Description	ATP-binding cassette, sub-family A (ABC1), member 8, transcript variant 2
Clone name	hh02681
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5677 bp) Predicted protein sequence (1591 aa)
HaloTag ORF Clone	FHC04008
Flexi ORF Clone	FXC04008
Source	Human adult brain
Rouge ID	mKIAA0822 by Kazusa Mouse cDNA Project

Length: 5677 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	793 bp
Genome contig ID	gi51511734r_64275028
PolyA signal sequence (AATAAA,-25)	+--------+--------+----*----+---- GAAAATGTGAAATAAATCTCATATATATAGTTTTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAATACGTTTTCTATTTCATGTGTCTTTTTCTTAACTCGGTGGCTTCCTG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	17	r	64375028	64463087	38	99.8	Perfect prediction

Length: 1591 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
O94911	100.0	ATP-binding cas...	Homo sapiens
XP_001082492	96.7	similar to ATP-...	Macaca mulatta
AAI30281	97.5	ABCA8 protein [...	Homo sapiens
EAW89076	57.5	hCG2039659, iso...	Homo sapiens
CAM13339	70.5	ATP-binding cas...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB067475	1.6e-87	42.1	KIAA1888
AB028985	4.5e-13	26.1	KIAA1062

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	604	629	PD000006	ABC transporter related
BlastProDom	IPR003439	1387	1430	PD000006	ABC transporter related
HMMPfam	IPR003439	519	661	PF00005	ABC transporter related
HMMPfam	IPR003439	1286	1464	PF00005	ABC transporter related
HMMSmart	IPR003593	518	662	SM00382	AAA+ ATPase
HMMSmart	IPR003593	1285	1465	SM00382	AAA+ ATPase
ProfileScan	IPR003439	490	685	PS50893	ABC transporter related
ProfileScan	IPR003439	1255	1488	PS50893	ABC transporter related

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	39	ESLMEWLNSLLLLLCLYIYPHSH	61	PRIMARY	23
	2	231	ITDLYLFSCIISFSSFIYYASV	252	PRIMARY	22
	3	275	FWLSWGLLYAGFIFIMALFLALV	297	PRIMARY	23
	4	306	LSGFMVVFSLFLLYGLSLVALAF	328	PRIMARY	23
	5	337	SFLTGLVVFLLTVFWGCLGFTSL	359	PRIMARY	23
	6	367	LEWILSLLSPFAFMLGMAQLLHL	389	SECONDARY	23
	7	407	LIVATNFMLAFDTCLYLALAIYF	429	SECONDARY	23
	8	832	ALLALLLILMAGFCPLLVEYTMV	854	PRIMARY	23
	9	985	QDNPIGFLAYIMFWLVLTSSCPP	1007	SECONDARY	23
	10	1037	FGQALVDVSLYFLVFVFIYLMSY	1059	PRIMARY	23
	11	1071	IHIIQIPCAVGYSFSLIFMTYVI	1093	PRIMARY	23
	12	1105	GIWSFCFYVVTVFSVAGFAFSIF	1127	PRIMARY	23
	13	1136	TFLIPPATMIGCLFLSSHLLFSS	1158	SECONDARY	23
	14	1167	VQPFLVFLIPFLHFIIFLFTLRC	1189	PRIMARY	23

Primer_f	TTTCTGTAAGCCAACTGTGTG
Primer_r	TTCTACCTACTCAGTCATGTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TTTCTGTAAGCCAACTGTGTG
Primer_r	TTCTACCTACTCAGTCATGTG
PCR product length	153 bp
PCR conditions	95 °C15 sec62 °C60 sec35 cycles