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Order Kazusa clone(s) from : |
| Product ID | ORK04592 |
|---|---|
| Accession No | AB020709 |
| Description | connector enhancer of kinase suppressor of Ras 2 |
| Clone name | hk09777 |
| Vector information | |
| cDNA sequence | DNA sequence (4349 bp) Predicted protein sequence (910 aa) |
| Source | Human adult brain |
| Rouge ID |
mKIAA0902
by Kazusa Mouse cDNA Project
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Length: 4349 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | Warning |
Integrity of 3' end
| Length of 3'UTR | 1211 bp |
|---|---|
| Genome contig ID | gi89161218f_21202481 |
| PolyA signal sequence (AATAAA,-27) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (368294 - 368343) |
----+----*----+----*----+----*----+----*----+----* |
Length: 910 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR001660 | 26 | 90 | PF00536 | Sterile alpha motif SAM |
| IPR001478 | 234 | 311 | PF00595 | PDZ/DHR/GLGF | |
| IPR010599 | 349 | 532 | PF06663 | Connector enhancer of kinase suppressor of ras 2 | |
| IPR001849 | 588 | 686 | PF00169 | Pleckstrin-like | |
| HMMSmart | IPR001660 | 25 | 93 | SM00454 | Sterile alpha motif SAM |
| IPR001478 | 242 | 314 | SM00228 | PDZ/DHR/GLGF | |
| IPR001849 | 588 | 688 | SM00233 | Pleckstrin-like | |
| ProfileScan | IPR001660 | 28 | 93 | PS50105 | Sterile alpha motif SAM |
| IPR001478 | 232 | 314 | PS50106 | PDZ/DHR/GLGF | |
| IPR001849 | 587 | 686 | PS50003 | Pleckstrin-like |
RT-PCR-ELISA
|
Experimental conditions| Primer_f | AATCTATAGGCTGTGGGTTTC |
|---|---|
| Primer_r | ATTGATTGAGCCATAGGGGAC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 1
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | AATCTATAGGCTGTGGGTTTC |
| Primer_r | ATTGATTGAGCCATAGGGGAC |
| PCR product length | 113 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |