KIAA1044

Product ID	ORK00720
Accession No	AB028967
Description	potassium channel, voltage gated Shal related subfamily D, member 2
Clone name	fh03989
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5333 bp) Predicted protein sequence (646 aa)
HaloTag ORF Clone	FHC00720
Flexi ORF Clone	FXC00720
Source	Human fetal brain
Rouge ID	mKIAA1044 by Kazusa Mouse cDNA Project

Length: 5333 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2475 bp
Genome contig ID	gi89161213f_119600958
PolyA signal sequence (AATAAA,-29)	+--------+--------+----*----+---- TACTTAAATAAAACATGTGCATGCTTGAACAGGAC
Flanking genome sequence (576667 - 576716)	----+--------+--------+--------+--------+----* AAAATGTTGACTGTTGCCCTATTTTCTTAGATTTCATTCCTTTCCCAAAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	7	f	119700958	120177623	6	99.5	Perfect prediction

Length: 646 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9NZV8	100.0	Potassium volta...	Homo sapiens
CAH90068	99.7	hypothetical pr...	Pongo abelii
XP_001251585	99.2	similar to pota...	Bos taurus
AAD22053	99.4	potassium chann...	Homo sapiens
AAF65618	99.7	voltage-gated p...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB032970	9.1e-26	30.3	KIAA1144
D79998	0.00026	31.5	KIAA0176

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR004055	46	58	PR01517	Kv4.2 voltage-gated K+ channel
	IPR003975	84	97	PR01497	Shal voltage-gated K+ channel
	IPR003968	98	108	PR01491	Kv channel
	IPR003091	98	117	PR00169	Voltage-dependent potassium channel
	IPR003975	159	175	PR01497	Shal voltage-gated K+ channel
	IPR004055	164	180	PR01517	Kv4.2 voltage-gated K+ channel
	IPR003975	177	191	PR01497	Shal voltage-gated K+ channel
	IPR003091	194	222	PR00169	Voltage-dependent potassium channel
	IPR003975	196	210	PR01497	Shal voltage-gated K+ channel
	IPR003975	236	252	PR01497	Shal voltage-gated K+ channel
	IPR003091	247	270	PR00169	Voltage-dependent potassium channel
	IPR003091	273	293	PR00169	Voltage-dependent potassium channel
	IPR003091	310	336	PR00169	Voltage-dependent potassium channel
	IPR003968	315	323	PR01491	Kv channel
	IPR003975	334	353	PR01497	Shal voltage-gated K+ channel
	IPR003968	339	353	PR01491	Kv channel
	IPR003091	339	362	PR00169	Voltage-dependent potassium channel
	IPR003091	370	392	PR00169	Voltage-dependent potassium channel
	IPR003091	399	425	PR00169	Voltage-dependent potassium channel
	IPR003968	410	421	PR01491	Kv channel
	IPR003975	431	442	PR01497	Shal voltage-gated K+ channel
	IPR003975	445	461	PR01497	Shal voltage-gated K+ channel
	IPR004055	477	489	PR01517	Kv4.2 voltage-gated K+ channel
	IPR003975	491	505	PR01497	Shal voltage-gated K+ channel
	IPR004055	576	589	PR01517	Kv4.2 voltage-gated K+ channel
	IPR004055	596	608	PR01517	Kv4.2 voltage-gated K+ channel
HMMPfam	IPR003131	59	148	PF02214	K+ channel tetramerisation
HMMPfam	IPR005821	247	421	PF00520	Ion transport
HMMSmart	IPR000210	57	156	SM00225	BTB/POZ-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	12	PLQVIMAAGVAAWLPFARAAAIG	34	PRIMARY	23
	2	201	LVFYYVTGFFIAVSVIANVVETV	223	SECONDARY	23
	3	242	AVAFFCLDTACVMIFTVEYLLRL	264	SECONDARY	23
	4	273	FVRSVMSIIDVVAILPYYIGLVM	295	PRIMARY	23
	5	337	ASELGFLLFSLTMAIIIFATVMF	359	PRIMARY	23
	6	369	KFTSIPAAFWYTIVTMTTLGYGD	391	SECONDARY	23
	7	402	FGSICSLSGVLVIALPVPVIVSN	424	PRIMARY	23

Primer_f	CCTAGATACTGTTACAACTGC
Primer_r	CTACGAATGTGGCTCTTACCG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CCTAGATACTGTTACAACTGC
Primer_r	CTACGAATGTGGCTCTTACCG
PCR product length	117 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles