KIAA1724

Product ID	ORK00903
Accession No	AB051511
Description	ethanolaminephosphotransferase 1
Clone name	pf00943
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (8082 bp) Predicted protein sequence (400 aa)
HaloTag ORF Clone	FHC00903
Flexi ORF Clone	FXC00903
Source	Human brain (hippocampus)
Rouge ID	mKIAA1724 by Kazusa Mouse cDNA Project

Length: 8082 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	6812 bp
Genome contig ID	gi89161199f_26322496
PolyA signal sequence (AATAAA,-34)	+--------+--------+----*----+---- TAATAAAAGTAAAAGAGTAATTTTTAAATCCTTTT
Flanking genome sequence (149761 - 149810)	----+--------+--------+--------+--------+----* AATTGTTTTCAGATTGTCTTCTTTGTTTTCCTTGGTAAGTGCAAAAGGGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	2	f	26422496	26472255	10	99.1	Perfect prediction

Length: 400 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EAX00690	3e-160	100.0	selenoprotein I...	Homo sapiens
Q9C0D9	3.1e-160	100.0	Ethanolaminepho...	Homo sapiens
NP_001129035	4.4e-159	99.2	selenoprotein I...	Macaca mulatta
Q5NV96	1.5e-158	99.2	Ethanolaminepho...	Pongo abelii
XP_001918226	4.5e-155	94.8	similar to Etha...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000462	108	252	PF01066	CDP-alcohol phosphatidyltransferase
ScanRegExp	IPR000462	121	143	PS00379	CDP-alcohol phosphatidyltransferase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	58	FPTWLAPNLITFSGFLLVVFNFL	80	PRIMARY	23
	2	99	VPDWVWIVVGILNFVAYTLDGVD	121	PRIMARY	23
	3	138	FDHGLDSWSCVYFVVTVYSIFGR	160	SECONDARY	23
	4	163	TGVSVFVLYLLLWVVLFSFILSH	185	PRIMARY	23
	5	195	FLPWGYDISQVTISFVYIVTAVV	217	SECONDARY	23
	6	236	FTAMIIGCALCVTLPMSLLNFF	257	PRIMARY	22
	7	269	SVYEAMVPLFSPCLLFILSTAWI	291	PRIMARY	23
	8	305	VFYFMVGTAFANSTCQLIVCQMS	327	SECONDARY	23
	9	341	LFLVVLVVNLGVASYVESILLYT	363	PRIMARY	23

Primer_f	ATGACTTGTTTCCCTATGGTG
Primer_r	GCACTTTCAAGAATGGCAGAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-